Restriction map for
pF1KSDA0341
(Linear) (Six-base) MAPSORT of: KIAA0341.seq Check: 4747 from: 1 to: 1632
KIAA0341.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 840 1632
Size: 840 792
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 273 606 910 1179 1222 1632
Size: 98 175 333 304 269 43 410
Fragments arranged by size:
410 333 304 269 175 98 43
AflIII A'CryG_T
Cuts at: 0 1538 1632
Size: 1538 94
AleI CACnn'nnGTG
Cuts at: 0 850 1632
Size: 850 782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1024 1369 1632
Size: 1024 345 263
Fragments arranged by size:
1024 345 263
AlwNI CAG_nnn'CTG
Cuts at: 0 19 423 513 855 873 1026 1066 1149
Size: 19 404 90 342 18 153 40 83
Cuts at: 1149 1182 1449 1560 1572 1632
Size: 33 267 111 12 60
Fragments arranged by size:
404 342 267 153 111 90 83 60
40 33 19 18 12
ApaI G_GGCC'C
Cuts at: 0 225 485 1047 1632
Size: 225 260 562 585
Fragments arranged by size:
585 562 260 225
ApaLI G'TGCA_C
Cuts at: 0 1076 1632
Size: 1076 556
AvaI C'yCGr_G
Cuts at: 0 107 478 485 500 550 652 724 787
Size: 107 371 7 15 50 102 72 63
Cuts at: 787 1040 1301 1615 1632
Size: 253 261 314 17
Fragments arranged by size:
371 314 261 253 107 102 72 63
50 17 15 7
BanI G'GyrC_C
Cuts at: 0 992 1051 1632
Size: 992 59 581
Fragments arranged by size:
992 581 59
BanII G_rGCy'C
Cuts at: 0 115 134 225 478 485 551 557 661
Size: 115 19 91 253 7 66 6 104
Cuts at: 661 806 1017 1047 1592 1632
Size: 145 211 30 545 40
Fragments arranged by size:
545 253 211 145 115 104 91 66
40 30 19 7 6
BbeI G_GCGC'C
Cuts at: 0 996 1632
Size: 996 636
BbsI GAAGACnn'nnnn_
Cuts at: 0 1123 1210 1632
Size: 1123 87 422
Fragments arranged by size:
1123 422 87
BbvCI CC'TCA_GC
Cuts at: 0 166 523 1632
Size: 166 357 1109
Fragments arranged by size:
1109 357 166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1282 1316 1632
Size: 1282 34 316
Fragments arranged by size:
1282 316 34
BglI GCCn_nnn'nGGC
Cuts at: 0 23 129 496 1632
Size: 23 106 367 1136
Fragments arranged by size:
1136 367 106 23
BglII A'GATC_T
Cuts at: 0 1190 1632
Size: 1190 442
BlpI GC'TnA_GC
Cuts at: 0 712 884 935 977 1016 1394 1409 1632
Size: 712 172 51 42 39 378 15 223
Fragments arranged by size:
712 378 223 172 51 42 39 15
Bme1580I G_kGCm'C
Cuts at: 0 225 485 507 1047 1080 1230 1581 1632
Size: 225 260 22 540 33 150 351 51
Fragments arranged by size:
540 351 260 225 150 51 33 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 1067 1632
Size: 1067 565
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 849 1632
Size: 849 783
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 558 877 940 1582 1623 1632
Size: 558 319 63 642 41 9
Fragments arranged by size:
642 558 319 63 41 9
Bpu10I CC'TnA_GC
Cuts at: 0 166 472 523 545 1241 1632
Size: 166 306 51 22 696 391
Fragments arranged by size:
696 391 306 166 51 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 790 1632
Size: 662 128 842
Fragments arranged by size:
842 662 128
BsaI GGTCTCn'nnnn_
Cuts at: 0 110 784 1632
Size: 110 674 848
Fragments arranged by size:
848 674 110
BsaHI Gr'CG_yC
Cuts at: 0 403 993 1632
Size: 403 590 639
Fragments arranged by size:
639 590 403
BsaWI w'CCGG_w
Cuts at: 0 137 1032 1632
Size: 137 895 600
Fragments arranged by size:
895 600 137
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 733 763 1632
Size: 733 30 869
Fragments arranged by size:
869 733 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 561 618 747 814 874 1102 1498
Size: 124 437 57 129 67 60 228 396
Cuts at: 1498 1632
Size: 134
Fragments arranged by size:
437 396 228 134 129 124 67 60
57
BseYI C'CCAG_C
Cuts at: 0 556 694 1632
Size: 556 138 938
Fragments arranged by size:
938 556 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 430 499 898 1632
Size: 160 270 69 399 734
Fragments arranged by size:
734 399 270 160 69
BsiHKAI G_wGCw'C
Cuts at: 0 205 806 1080 1632
Size: 205 601 274 552
Fragments arranged by size:
601 552 274 205
BsmI GAATG_Cn'
Cuts at: 0 335 1632
Size: 335 1297
Bsp1286I G_dGCh'C
Cuts at: 0 115 134 205 225 478 485 507 551
Size: 115 19 71 20 253 7 22 44
Cuts at: 551 557 661 806 1017 1047 1080 1230 1581
Size: 6 104 145 211 30 33 150 351
Cuts at: 1581 1592 1632
Size: 11 40
Fragments arranged by size:
351 253 211 150 145 115 104 71
44 40 33 30 22 20 19 11
7 6
BspEI T'CCGG_A
Cuts at: 0 137 1032 1632
Size: 137 895 600
Fragments arranged by size:
895 600 137
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 182 840 1632
Size: 182 658 792
Fragments arranged by size:
792 658 182
BsrBI CCG'CTC
Cuts at: 0 909 1467 1632
Size: 909 558 165
Fragments arranged by size:
909 558 165
BsrDI GCAATG_nn'
Cuts at: 0 26 441 1632
Size: 26 415 1191
Fragments arranged by size:
1191 415 26
BssSI C'ACGA_G
Cuts at: 0 916 1632
Size: 916 716
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1149 1449 1572 1632
Size: 1149 300 123 60
Fragments arranged by size:
1149 300 123 60
BstEII G'GTnAC_C
Cuts at: 0 921 1632
Size: 921 711
BstXI CCAn_nnnn'nTGG
Cuts at: 0 127 1632
Size: 127 1505
BstYI r'GATC_y
Cuts at: 0 1190 1517 1632
Size: 1190 327 115
Fragments arranged by size:
1190 327 115
BtgI C'CryG_G
Cuts at: 0 32 85 1632
Size: 32 53 1547
Fragments arranged by size:
1547 53 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 836 1632
Size: 836 796
BtsI GCAGTG_nn'
Cuts at: 0 1579 1632
Size: 1579 53
DraIII CAC_nnn'GTG
Cuts at: 0 921 1632
Size: 921 711
EaeI y'GGCC_r
Cuts at: 0 2 636 1632
Size: 2 634 996
Fragments arranged by size:
996 634 2
EarI CTCTTCn'nnn_
Cuts at: 0 74 1632
Size: 74 1558
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1180 1201 1485 1632
Size: 1180 21 284 147
Fragments arranged by size:
1180 284 147 21
EcoICRI GAG'CTC
Cuts at: 0 804 1632
Size: 804 828
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 273 558 606 877 910 940 1179
Size: 98 175 285 48 271 33 30 239
Cuts at: 1179 1222 1582 1623 1632
Size: 43 360 41 9
Fragments arranged by size:
360 285 271 239 175 98 48 43
41 33 30 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 139 532 1000 1060 1632
Size: 139 393 468 60 572
Fragments arranged by size:
572 468 393 139 60
EcoO109I rG'GnC_Cy
Cuts at: 0 16 222 482 682 813 1023 1044 1296
Size: 16 206 260 200 131 210 21 252
Cuts at: 1296 1350 1632
Size: 54 282
Fragments arranged by size:
282 260 252 210 206 200 131 54
21 16
FspI TGC'GCA
Cuts at: 0 1000 1279 1632
Size: 1000 279 353
Fragments arranged by size:
1000 353 279
HaeII r_GCGC'y
Cuts at: 0 867 897 996 1450 1632
Size: 867 30 99 454 182
Fragments arranged by size:
867 454 182 99 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 658 690 1182 1214 1632
Size: 658 32 492 32 418
Fragments arranged by size:
658 492 418 32 32
KasI G'GCGC_C
Cuts at: 0 992 1632
Size: 992 640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 590 860 1632
Size: 35 555 270 772
Fragments arranged by size:
772 555 270 35
MscI TGG'CCA
Cuts at: 0 4 638 1632
Size: 4 634 994
Fragments arranged by size:
994 634 4
MslI CAynn'nnrTG
Cuts at: 0 31 850 1537 1632
Size: 31 819 687 95
Fragments arranged by size:
819 687 95 31
MspA1I CmG'CkG
Cuts at: 0 87 510 706 870 960 966 990 1149
Size: 87 423 196 164 90 6 24 159
Cuts at: 1149 1322 1401 1512 1632
Size: 173 79 111 120
Fragments arranged by size:
423 196 173 164 159 120 111 90
87 79 24 6
NarI GG'CG_CC
Cuts at: 0 993 1632
Size: 993 639
NcoI C'CATG_G
Cuts at: 0 32 1632
Size: 32 1600
NspI r_CATG'y
Cuts at: 0 1542 1632
Size: 1542 90
PciI A'CATG_T
Cuts at: 0 1538 1632
Size: 1538 94
PflMI CCAn_nnn'nTGG
Cuts at: 0 1182 1560 1601 1632
Size: 1182 378 41 31
Fragments arranged by size:
1182 378 41 31
PpuMI rG'GwC_Cy
Cuts at: 0 682 1632
Size: 682 950
PspOMI G'GGCC_C
Cuts at: 0 221 481 1043 1632
Size: 221 260 562 589
Fragments arranged by size:
589 562 260 221
PspXI vC'TCGA_Gb
Cuts at: 0 1615 1632
Size: 1615 17
PstI C_TGCA'G
Cuts at: 0 965 1289 1421 1632
Size: 965 324 132 211
Fragments arranged by size:
965 324 211 132
PvuII CAG'CTG
Cuts at: 0 510 706 960 966 1149 1322 1401 1632
Size: 510 196 254 6 183 173 79 231
Fragments arranged by size:
510 254 231 196 183 173 79 6
SacI G_AGCT'C
Cuts at: 0 806 1632
Size: 806 826
SacII CC_GC'GG
Cuts at: 0 88 1632
Size: 88 1544
SapI GCTCTTCn'nnn_
Cuts at: 0 74 1632
Size: 74 1558
SbfI CC_TGCA'GG
Cuts at: 0 1289 1632
Size: 1289 343
SfcI C'TryA_G
Cuts at: 0 961 1285 1417 1632
Size: 961 324 132 215
Fragments arranged by size:
961 324 215 132
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 496 1632
Size: 496 1136
SfoI GGC'GCC
Cuts at: 0 994 1632
Size: 994 638
SmaI CCC'GGG
Cuts at: 0 480 487 502 1632
Size: 480 7 15 1130
Fragments arranged by size:
1130 480 15 7
SmlI C'TyrA_G
Cuts at: 0 677 805 1615 1632
Size: 677 128 810 17
Fragments arranged by size:
810 677 128 17
SrfI GCCC'GGGC
Cuts at: 0 502 1632
Size: 502 1130
StyI C'CwwG_G
Cuts at: 0 32 633 686 816 1632
Size: 32 601 53 130 816
Fragments arranged by size:
816 601 130 53 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 39 396 1632
Size: 39 357 1236
Fragments arranged by size:
1236 357 39
XhoI C'TCGA_G
Cuts at: 0 1615 1632
Size: 1615 17
XmaI C'CCGG_G
Cuts at: 0 478 485 500 1632
Size: 478 7 15 1132
Fragments arranged by size:
1132 478 15 7
XmnI GAAnn'nnTTC
Cuts at: 0 379 1632
Size: 379 1253
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BssSI BstAPI
BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NarI NcoI
NspI PciI PflMI PpuMI PspOMI PspXI PstI PvuII
SacI SacII SapI SbfI SfcI SfiI SfoI SmaI
SmlI SrfI StyI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BclI BfrBI BmgBI BmtI BsaAI BsaBI BsiEI
BsiWI BsmBI BspHI BsrFI BsrGI BssHII BstBI BstZ17I
Bsu36I ClaI CspCI DraI DrdI EagI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI RsrII SalI SanDI ScaI SexAI SgfI
SgrAI SnaBI SpeI SphI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI ZraI