Restriction map for
pF1KA0343
(Linear) (Six-base) MAPSORT of: KIAA0343.seq Check: 8535 from: 1 to: 3540
KIAA0343.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1326 3099 3540
Size: 1326 1773 441
Fragments arranged by size:
1773 1326 441
AatII G_ACGT'C
Cuts at: 0 2634 3540
Size: 2634 906
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1708 2057 2266 3335 3503 3540
Size: 1708 349 209 1069 168 37
Fragments arranged by size:
1708 1069 349 209 168 37
AfeI AGC'GCT
Cuts at: 0 31 1827 3540
Size: 31 1796 1713
Fragments arranged by size:
1796 1713 31
AflIII A'CryG_T
Cuts at: 0 1513 1784 1786 3540
Size: 1513 271 2 1754
Fragments arranged by size:
1754 1513 271 2
AleI CACnn'nnGTG
Cuts at: 0 435 813 1789 2197 3540
Size: 435 378 976 408 1343
Fragments arranged by size:
1343 976 435 408 378
AlwNI CAG_nnn'CTG
Cuts at: 0 946 2221 2412 3540
Size: 946 1275 191 1128
Fragments arranged by size:
1275 1128 946 191
ApaI G_GGCC'C
Cuts at: 0 2755 3540
Size: 2755 785
ApaLI G'TGCA_C
Cuts at: 0 83 2533 3540
Size: 83 2450 1007
Fragments arranged by size:
2450 1007 83
ApoI r'AATT_y
Cuts at: 0 1475 1978 2957 2991 2997 3528 3540
Size: 1475 503 979 34 6 531 12
Fragments arranged by size:
1475 979 531 503 34 12 6
AvaI C'yCGr_G
Cuts at: 0 179 2434 3540
Size: 179 2255 1106
Fragments arranged by size:
2255 1106 179
BamHI G'GATC_C
Cuts at: 0 2859 3540
Size: 2859 681
BanI G'GyrC_C
Cuts at: 0 2024 2300 3504 3540
Size: 2024 276 1204 36
Fragments arranged by size:
2024 1204 276 36
BanII G_rGCy'C
Cuts at: 0 2702 2755 3540
Size: 2702 53 785
Fragments arranged by size:
2702 785 53
BbeI G_GCGC'C
Cuts at: 0 2304 3540
Size: 2304 1236
BbsI GAAGACnn'nnnn_
Cuts at: 0 125 647 2474 2608 3320 3540
Size: 125 522 1827 134 712 220
Fragments arranged by size:
1827 712 522 220 134 125
BciVI GTATCCnnnnn_n'
Cuts at: 0 2361 3540
Size: 2361 1179
BclI T'GATC_A
Cuts at: 0 1656 2396 3540
Size: 1656 740 1144
Fragments arranged by size:
1656 1144 740
BfrBI ATG'CAT
Cuts at: 0 976 3540
Size: 976 2564
BlpI GC'TnA_GC
Cuts at: 0 1295 1834 3540
Size: 1295 539 1706
Fragments arranged by size:
1706 1295 539
Bme1580I G_kGCm'C
Cuts at: 0 53 87 2537 2755 3540
Size: 53 34 2450 218 785
Fragments arranged by size:
2450 785 218 53 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 861 1703 2218 2539 2547 2792 3540
Size: 861 842 515 321 8 245 748
Fragments arranged by size:
861 842 748 515 321 245 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 599 2488 3453 3540
Size: 599 1889 965 87
Fragments arranged by size:
1889 965 599 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 832 1844 2483 2768 3540
Size: 743 89 1012 639 285 772
Fragments arranged by size:
1012 772 743 639 285 89
Bpu10I CC'TnA_GC
Cuts at: 0 2523 3540
Size: 2523 1017
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 3540
Size: 569 2971
BsaI GGTCTCn'nnnn_
Cuts at: 0 337 1388 3540
Size: 337 1051 2152
Fragments arranged by size:
2152 1051 337
BsaAI yAC'GTr
Cuts at: 0 1514 1787 2085 2240 3540
Size: 1514 273 298 155 1300
Fragments arranged by size:
1514 1300 298 273 155
BsaHI Gr'CG_yC
Cuts at: 0 2146 2301 2631 3540
Size: 2146 155 330 909
Fragments arranged by size:
2146 909 330 155
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 380 410 1801 1831 1842 1848 1872 1878
Size: 380 30 1391 30 11 6 24 6
Cuts at: 1878 3395 3425 3540
Size: 1517 30 115
Fragments arranged by size:
1517 1391 380 115 30 30 30 24
11 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1376 3540
Size: 1376 2164
BseYI C'CCAG_C
Cuts at: 0 1165 2063 2137 2754 3540
Size: 1165 898 74 617 786
Fragments arranged by size:
1165 898 786 617 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1244 3540
Size: 1244 2296
BsiHKAI G_wGCw'C
Cuts at: 0 87 1431 2537 2805 3540
Size: 87 1344 1106 268 735
Fragments arranged by size:
1344 1106 735 268 87
BsmI GAATG_Cn'
Cuts at: 0 875 1645 3540
Size: 875 770 1895
Fragments arranged by size:
1895 875 770
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1821 3540
Size: 1821 1719
Bsp1286I G_dGCh'C
Cuts at: 0 53 87 1431 2537 2702 2755 2805 3540
Size: 53 34 1344 1106 165 53 50 735
Fragments arranged by size:
1344 1106 735 165 53 53 50 34
BspHI T'CATG_A
Cuts at: 0 323 1434 3540
Size: 323 1111 2106
Fragments arranged by size:
2106 1111 323
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1326 3099 3540
Size: 1326 1773 441
Fragments arranged by size:
1773 1326 441
BsrDI GCAATG_nn'
Cuts at: 0 774 818 1245 1340 2009 3540
Size: 774 44 427 95 669 1531
Fragments arranged by size:
1531 774 669 427 95 44
BsrFI r'CCGG_y
Cuts at: 0 3104 3477 3540
Size: 3104 373 63
Fragments arranged by size:
3104 373 63
BsrGI T'GTAC_A
Cuts at: 0 367 3540
Size: 367 3173
BstAPI GCAn_nnn'nTGC
Cuts at: 0 973 3540
Size: 973 2567
BstBI TT'CG_AA
Cuts at: 0 2261 3540
Size: 2261 1279
BstEII G'GTnAC_C
Cuts at: 0 282 3540
Size: 282 3258
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2021 2199 2311 2878 3540
Size: 2021 178 112 567 662
Fragments arranged by size:
2021 662 567 178 112
BstYI r'GATC_y
Cuts at: 0 2648 2859 3540
Size: 2648 211 681
Fragments arranged by size:
2648 681 211
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1967 3109 3540
Size: 1967 1142 431
Fragments arranged by size:
1967 1142 431
BtsI GCAGTG_nn'
Cuts at: 0 2117 3540
Size: 2117 1423
ClaI AT'CG_AT
Cuts at: 0 266 3540
Size: 266 3274
DraI TTT'AAA
Cuts at: 0 1410 2987 2996 3540
Size: 1410 1577 9 544
Fragments arranged by size:
1577 1410 544 9
DraIII CAC_nnn'GTG
Cuts at: 0 571 3540
Size: 571 2969
DrdI GACnn_nn'nnGTC
Cuts at: 0 280 3540
Size: 280 3260
EaeI y'GGCC_r
Cuts at: 0 1793 3417 3540
Size: 1793 1624 123
Fragments arranged by size:
1793 1624 123
EarI CTCTTCn'nnn_
Cuts at: 0 67 1436 2122 3540
Size: 67 1369 686 1418
Fragments arranged by size:
1418 1369 686 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 406 1809 3540
Size: 406 1403 1731
Fragments arranged by size:
1731 1403 406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 832 1708 1844 2057 2266 2483 2768
Size: 743 89 876 136 213 209 217 285
Cuts at: 2768 3335 3503 3540
Size: 567 168 37
Fragments arranged by size:
876 743 567 285 217 213 209 168
136 89 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 299 625 3540
Size: 299 326 2915
Fragments arranged by size:
2915 326 299
EcoO109I rG'GnC_Cy
Cuts at: 0 600 1076 1778 2409 2541 2751 2938 3540
Size: 600 476 702 631 132 210 187 602
Fragments arranged by size:
702 631 602 600 476 210 187 132
EcoRI G'AATT_C
Cuts at: 0 3528 3540
Size: 3528 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 48 115 469 3540
Size: 48 67 354 3071
Fragments arranged by size:
3071 354 67 48
HaeII r_GCGC'y
Cuts at: 0 33 1829 2304 3540
Size: 33 1796 475 1236
Fragments arranged by size:
1796 1236 475 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1801 1833 2811 2843 3540
Size: 1801 32 978 32 697
Fragments arranged by size:
1801 978 697 32 32
HincII GTy'rAC
Cuts at: 0 1127 1731 3396 3519 3540
Size: 1127 604 1665 123 21
Fragments arranged by size:
1665 1127 604 123 21
HindIII A'AGCT_T
Cuts at: 0 228 3540
Size: 228 3312
HpaI GTT'AAC
Cuts at: 0 1127 3540
Size: 1127 2413
KasI G'GCGC_C
Cuts at: 0 2300 3540
Size: 2300 1240
MfeI C'AATT_G
Cuts at: 0 509 3540
Size: 509 3031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 1879 2854 3540
Size: 160 1719 975 686
Fragments arranged by size:
1719 975 686 160
MscI TGG'CCA
Cuts at: 0 1795 3419 3540
Size: 1795 1624 121
Fragments arranged by size:
1795 1624 121
MslI CAynn'nnrTG
Cuts at: 0 435 813 1460 1789 2197 2876 3540
Size: 435 378 647 329 408 679 664
Fragments arranged by size:
679 664 647 435 408 378 329
MspA1I CmG'CkG
Cuts at: 0 1775 1935 2067 2197 2443 3540
Size: 1775 160 132 130 246 1097
Fragments arranged by size:
1775 1097 246 160 132 130
NaeI GCC'GGC
Cuts at: 0 3106 3479 3540
Size: 3106 373 61
Fragments arranged by size:
3106 373 61
NarI GG'CG_CC
Cuts at: 0 2301 3540
Size: 2301 1239
NgoMIV G'CCGG_C
Cuts at: 0 3104 3477 3540
Size: 3104 373 63
Fragments arranged by size:
3104 373 63
NsiI A_TGCA'T
Cuts at: 0 978 3540
Size: 978 2562
NspI r_CATG'y
Cuts at: 0 2685 3540
Size: 2685 855
PflMI CCAn_nnn'nTGG
Cuts at: 0 1403 3540
Size: 1403 2137
PfoI T'CCnGG_A
Cuts at: 0 235 3540
Size: 235 3305
PmlI CAC'GTG
Cuts at: 0 1787 3540
Size: 1787 1753
PpuMI rG'GwC_Cy
Cuts at: 0 600 1076 1778 2541 3540
Size: 600 476 702 763 999
Fragments arranged by size:
999 763 702 600 476
PsiI TTA'TAA
Cuts at: 0 896 3540
Size: 896 2644
PspOMI G'GGCC_C
Cuts at: 0 2751 3540
Size: 2751 789
PstI C_TGCA'G
Cuts at: 0 396 502 1090 3540
Size: 396 106 588 2450
Fragments arranged by size:
2450 588 396 106
PvuII CAG'CTG
Cuts at: 0 1935 2067 2197 2443 3540
Size: 1935 132 130 246 1097
Fragments arranged by size:
1935 1097 246 132 130
SanDI GG'GwC_CC
Cuts at: 0 2541 3540
Size: 2541 999
SapI GCTCTTCn'nnn_
Cuts at: 0 1436 2122 3540
Size: 1436 686 1418
Fragments arranged by size:
1436 1418 686
ScaI AGT'ACT
Cuts at: 0 491 3540
Size: 491 3049
SexAI A'CCwGG_T
Cuts at: 0 1334 3540
Size: 1334 2206
SfcI C'TryA_G
Cuts at: 0 392 498 1086 2444 3540
Size: 392 106 588 1358 1096
Fragments arranged by size:
1358 1096 588 392 106
SfoI GGC'GCC
Cuts at: 0 2302 3540
Size: 2302 1238
SmlI C'TyrA_G
Cuts at: 0 584 3540
Size: 584 2956
SspI AAT'ATT
Cuts at: 0 189 533 3540
Size: 189 344 3007
Fragments arranged by size:
3007 344 189
StuI AGG'CCT
Cuts at: 0 1353 2279 3540
Size: 1353 926 1261
Fragments arranged by size:
1353 1261 926
StyI C'CwwG_G
Cuts at: 0 2661 3540
Size: 2661 879
SwaI ATTT'AAAT
Cuts at: 0 2996 3540
Size: 2996 544
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 160 3540
Size: 160 3380
TatI w'GTAC_w
Cuts at: 0 367 489 1498 2285 2894 3540
Size: 367 122 1009 787 609 646
Fragments arranged by size:
1009 787 646 609 367 122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1292 1324 1651 1683 2484 2516 2708 2740
Size: 1292 32 327 32 801 32 192 32
Cuts at: 2740 3540
Size: 800
Fragments arranged by size:
1292 801 800 327 192 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1813 2770 3540
Size: 1813 957 770
Fragments arranged by size:
1813 957 770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 233 848 1400 1803 3540
Size: 233 615 552 403 1737
Fragments arranged by size:
1737 615 552 403 233
XmnI GAAnn'nnTTC
Cuts at: 0 2257 3348 3540
Size: 2257 1091 192
Fragments arranged by size:
2257 1091 192
ZraI GAC'GTC
Cuts at: 0 2632 3540
Size: 2632 908
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AleI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BfrBI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrGI BstAPI BstBI BstEII BstXI BstYI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII HpaI KasI MfeI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PflMI PfoI PmlI
PpuMI PsiI PspOMI PstI PvuII SanDI SapI ScaI
SexAI SfcI SfoI SmlI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AlfI AloI
AscI AseI AsiSI AvrII BaeI BbvCI BcgI BglI
BglII BmgBI BmtI BsaBI BsaWI BsiEI BsiWI BspEI
BsrBI BssHII BssSI BstZ17I Bsu36I BtgI CspCI EagI
EcoICRI EcoRV FseI FspI FspAI KpnI MluI NcoI
NdeI NheI NotI NruI PacI PasI PciI PmeI
PpiI PshAI PspXI PsrI PvuI RsrII SacI SacII
SalI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI XbaI XhoI XmaI