Restriction map for
pF1KA0348
(Linear) (Six-base) MAPSORT of: KIAA0348.seq Check: 120 from: 1 to: 4488
KIAA0348.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 882 3177 4043 4122 4452 4488
Size: 882 2295 866 79 330 36
Fragments arranged by size:
2295 882 866 330 79 36
AatII G_ACGT'C
Cuts at: 0 1466 4488
Size: 1466 3022
AccI GT'mk_AC
Cuts at: 0 1466 4488
Size: 1466 3022
Acc65I G'GTAC_C
Cuts at: 0 3993 4488
Size: 3993 495
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 565 775 892 1390 1816 3353 4352
Size: 304 261 210 117 498 426 1537 999
Cuts at: 4352 4488
Size: 136
Fragments arranged by size:
1537 999 498 426 304 261 210 136
117
AflIII A'CryG_T
Cuts at: 0 176 4488
Size: 176 4312
AgeI A'CCGG_T
Cuts at: 0 3343 4488
Size: 3343 1145
AhdI GACnn_n'nnGTC
Cuts at: 0 1556 3024 4353 4488
Size: 1556 1468 1329 135
Fragments arranged by size:
1556 1468 1329 135
AleI CACnn'nnGTG
Cuts at: 0 259 659 4488
Size: 259 400 3829
Fragments arranged by size:
3829 400 259
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 4130 4488
Size: 34 4096 358
Fragments arranged by size:
4096 358 34
AlwNI CAG_nnn'CTG
Cuts at: 0 280 2036 2805 4044 4488
Size: 280 1756 769 1239 444
Fragments arranged by size:
1756 1239 769 444 280
ApaI G_GGCC'C
Cuts at: 0 2659 3800 4488
Size: 2659 1141 688
Fragments arranged by size:
2659 1141 688
ApoI r'AATT_y
Cuts at: 0 269 1567 1575 2254 2291 3073 4488
Size: 269 1298 8 679 37 782 1415
Fragments arranged by size:
1415 1298 782 679 269 37 8
AvaI C'yCGr_G
Cuts at: 0 375 1204 1695 2618 3106 3842 3999 4488
Size: 375 829 491 923 488 736 157 489
Fragments arranged by size:
923 829 736 491 489 488 375 157
BamHI G'GATC_C
Cuts at: 0 1535 1586 4488
Size: 1535 51 2902
Fragments arranged by size:
2902 1535 51
BanI G'GyrC_C
Cuts at: 0 32 1996 2518 3391 3565 3666 3972 3993
Size: 32 1964 522 873 174 101 306 21
Cuts at: 3993 4106 4123 4186 4488
Size: 113 17 63 302
Fragments arranged by size:
1964 873 522 306 302 174 113 101
63 32 21 17
BanII G_rGCy'C
Cuts at: 0 1341 1383 2659 2744 3230 3632 3800 4300
Size: 1341 42 1276 85 486 402 168 500
Cuts at: 4300 4330 4488
Size: 30 158
Fragments arranged by size:
1341 1276 500 486 402 168 158 85
42 30
BbeI G_GCGC'C
Cuts at: 0 36 2000 3670 3976 4488
Size: 36 1964 1670 306 512
Fragments arranged by size:
1964 1670 512 306 36
BbsI GAAGACnn'nnnn_
Cuts at: 0 1091 1969 2099 2205 2902 3053 3062 4122
Size: 1091 878 130 106 697 151 9 1060
Cuts at: 4122 4488
Size: 366
Fragments arranged by size:
1091 1060 878 697 366 151 130 106
9
BbvCI CC'TCA_GC
Cuts at: 0 199 370 3446 4488
Size: 199 171 3076 1042
Fragments arranged by size:
3076 1042 199 171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2596 2630 4488
Size: 2596 34 1858
Fragments arranged by size:
2596 1858 34
BclI T'GATC_A
Cuts at: 0 995 2259 2559 4488
Size: 995 1264 300 1929
Fragments arranged by size:
1929 1264 995 300
BglI GCCn_nnn'nGGC
Cuts at: 0 124 510 980 3523 4488
Size: 124 386 470 2543 965
Fragments arranged by size:
2543 965 470 386 124
BlpI GC'TnA_GC
Cuts at: 0 2355 4488
Size: 2355 2133
Bme1580I G_kGCm'C
Cuts at: 0 506 2523 2659 3800 3989 4109 4126 4488
Size: 506 2017 136 1141 189 120 17 362
Fragments arranged by size:
2017 1141 506 362 189 136 120 17
BmgBI CAC'GTC
Cuts at: 0 221 4488
Size: 221 4267
BmrI ACTGGGnnnn_n'
Cuts at: 0 1089 4345 4431 4488
Size: 1089 3256 86 57
Fragments arranged by size:
3256 1089 86 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3136 3299 4488
Size: 3136 163 1189
Fragments arranged by size:
3136 1189 163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 113 2471 3010 3988 4051 4488
Size: 88 25 2358 539 978 63 437
Fragments arranged by size:
2358 978 539 437 88 63 25
Bpu10I CC'TnA_GC
Cuts at: 0 7 199 370 1312 1648 3446 4488
Size: 7 192 171 942 336 1798 1042
Fragments arranged by size:
1798 1042 942 336 192 171 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 1077 1210 3214 3747 4488
Size: 528 549 133 2004 533 741
Fragments arranged by size:
2004 741 549 533 528 133
BsaI GGTCTCn'nnnn_
Cuts at: 0 416 1042 1298 2516 3096 3287 3321 3649
Size: 416 626 256 1218 580 191 34 328
Cuts at: 3649 4488
Size: 839
Fragments arranged by size:
1218 839 626 580 416 328 256 191
34
BsaAI yAC'GTr
Cuts at: 0 501 4488
Size: 501 3987
BsaHI Gr'CG_yC
Cuts at: 0 33 1463 1892 1997 3667 3973 4488
Size: 33 1430 429 105 1670 306 515
Fragments arranged by size:
1670 1430 515 429 306 105 33
BsaWI w'CCGG_w
Cuts at: 0 1589 1634 3343 3539 3753 4488
Size: 1589 45 1709 196 214 735
Fragments arranged by size:
1709 1589 735 214 196 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 111 141 917 947 2496 2526 3938 3968
Size: 111 30 776 30 1549 30 1412 30
Cuts at: 3968 4488
Size: 520
Fragments arranged by size:
1549 1412 776 520 111 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 352 943 1489 2941 3143 3375 3575 4017
Size: 352 591 546 1452 202 232 200 442
Cuts at: 4017 4488
Size: 471
Fragments arranged by size:
1452 591 546 471 442 352 232 202
200
BseYI C'CCAG_C
Cuts at: 0 27 1688 1728 3157 4488
Size: 27 1661 40 1429 1331
Fragments arranged by size:
1661 1429 1331 40 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 1060 1207 4488
Size: 237 823 147 3281
Fragments arranged by size:
3281 823 237 147
BsiEI CG_ry'CG
Cuts at: 0 81 2063 2582 4488
Size: 81 1982 519 1906
Fragments arranged by size:
1982 1906 519 81
BsiHKAI G_wGCw'C
Cuts at: 0 938 1649 2619 2744 3230 3444 4300 4488
Size: 938 711 970 125 486 214 856 188
Fragments arranged by size:
970 938 856 711 486 214 188 125
BsiWI C'GTAC_G
Cuts at: 0 179 4488
Size: 179 4309
BsmBI CGTCTCn'nnnn_
Cuts at: 0 227 621 3107 4488
Size: 227 394 2486 1381
Fragments arranged by size:
2486 1381 394 227
Bsp1286I G_dGCh'C
Cuts at: 0 506 938 1341 1383 1649 2523 2619 2659
Size: 506 432 403 42 266 874 96 40
Cuts at: 2659 2744 3230 3444 3632 3800 3989 4109 4126
Size: 85 486 214 188 168 189 120 17
Cuts at: 4126 4300 4330 4488
Size: 174 30 158
Fragments arranged by size:
874 506 486 432 403 266 214 189
188 174 168 158 120 96 85 42
40 30 17
BspEI T'CCGG_A
Cuts at: 0 1589 1634 4488
Size: 1589 45 2854
Fragments arranged by size:
2854 1589 45
BspHI T'CATG_A
Cuts at: 0 2151 4398 4488
Size: 2151 2247 90
Fragments arranged by size:
2247 2151 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 882 2738 3177 3269 4029 4043 4122 4452
Size: 882 1856 439 92 760 14 79 330
Cuts at: 4452 4488
Size: 36
Fragments arranged by size:
1856 882 760 439 330 92 79 36
14
BsrBI CCG'CTC
Cuts at: 0 2085 4488
Size: 2085 2403
BsrFI r'CCGG_y
Cuts at: 0 106 3299 3343 4488
Size: 106 3193 44 1145
Fragments arranged by size:
3193 1145 106 44
BssHII G'CGCG_C
Cuts at: 0 73 4488
Size: 73 4415
BstAPI GCAn_nnn'nTGC
Cuts at: 0 864 4044 4488
Size: 864 3180 444
Fragments arranged by size:
3180 864 444
BstEII G'GTnAC_C
Cuts at: 0 564 4488
Size: 564 3924
BstXI CCAn_nnnn'nTGG
Cuts at: 0 661 696 3147 4488
Size: 661 35 2451 1341
Fragments arranged by size:
2451 1341 661 35
BstYI r'GATC_y
Cuts at: 0 1535 1586 4338 4488
Size: 1535 51 2752 150
Fragments arranged by size:
2752 1535 150 51
Bsu36I CC'TnA_GG
Cuts at: 0 4413 4488
Size: 4413 75
BtgI C'CryG_G
Cuts at: 0 660 3396 3590 4118 4488
Size: 660 2736 194 528 370
Fragments arranged by size:
2736 660 528 370 194
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1182 4196 4210 4488
Size: 1182 3014 14 278
Fragments arranged by size:
3014 1182 278 14
BtsI GCAGTG_nn'
Cuts at: 0 296 2885 2969 4095 4456 4488
Size: 296 2589 84 1126 361 32
Fragments arranged by size:
2589 1126 361 296 84 32
DraIII CAC_nnn'GTG
Cuts at: 0 522 864 4425 4488
Size: 522 342 3561 63
Fragments arranged by size:
3561 522 342 63
DrdI GACnn_nn'nnGTC
Cuts at: 0 427 2494 4488
Size: 427 2067 1994
Fragments arranged by size:
2067 1994 427
EaeI y'GGCC_r
Cuts at: 0 78 116 397 678 1302 2060 3307 4488
Size: 78 38 281 281 624 758 1247 1181
Fragments arranged by size:
1247 1181 758 624 281 281 78 38
EagI C'GGCC_G
Cuts at: 0 78 2060 4488
Size: 78 1982 2428
Fragments arranged by size:
2428 1982 78
EarI CTCTTCn'nnn_
Cuts at: 0 320 323 1751 2699 4488
Size: 320 3 1428 948 1789
Fragments arranged by size:
1789 1428 948 320 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 938 1672 3250 4488
Size: 938 734 1578 1238
Fragments arranged by size:
1578 1238 938 734
EcoICRI GAG'CTC
Cuts at: 0 2742 3228 4298 4488
Size: 2742 486 1070 190
Fragments arranged by size:
2742 1070 486 190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 113 304 565 775 892 1390 1816
Size: 88 25 191 261 210 117 498 426
Cuts at: 1816 2471 3010 3353 3988 4051 4352 4488
Size: 655 539 343 635 63 301 136
Fragments arranged by size:
655 635 539 498 426 343 301 261
210 191 136 117 88 63 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1315 3729 3941 4488
Size: 1315 2414 212 547
Fragments arranged by size:
2414 1315 547 212
EcoO109I rG'GnC_Cy
Cuts at: 0 1209 1604 2655 2656 2727 3603 3797 3819
Size: 1209 395 1051 1 71 876 194 22
Cuts at: 3819 4041 4071 4236 4488
Size: 222 30 165 252
Fragments arranged by size:
1209 1051 876 395 252 222 194 165
71 30 22 1
EcoRI G'AATT_C
Cuts at: 0 269 1567 3073 4488
Size: 269 1298 1506 1415
Fragments arranged by size:
1506 1415 1298 269
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3500 3741 4488
Size: 3500 241 747
Fragments arranged by size:
3500 747 241
HaeII r_GCGC'y
Cuts at: 0 36 1519 2000 3670 3976 4367 4488
Size: 36 1483 481 1670 306 391 121
Fragments arranged by size:
1670 1483 481 391 306 121 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 442 474 718 750 1140 1172 1542 1546
Size: 442 32 244 32 390 32 370 4
Cuts at: 1546 1574 1578 2338 2370 2812 2844 2915 2947
Size: 28 4 760 32 442 32 71 32
Cuts at: 2947 3010 3042 3106 3138 3825 3857 4084 4116
Size: 63 32 64 32 687 32 227 32
Cuts at: 4116 4339 4371 4488
Size: 223 32 117
Fragments arranged by size:
760 687 442 442 390 370 244 227
223 117 71 64 63 32 32 32
32 32 32 32 32 32 32 32
28 4 4
HincII GTy'rAC
Cuts at: 0 1260 1871 2487 4488
Size: 1260 611 616 2001
Fragments arranged by size:
2001 1260 616 611
KasI G'GCGC_C
Cuts at: 0 32 1996 3666 3972 4488
Size: 32 1964 1670 306 516
Fragments arranged by size:
1964 1670 516 306 32
KpnI G_GTAC'C
Cuts at: 0 3997 4488
Size: 3997 491
MfeI C'AATT_G
Cuts at: 0 2771 4488
Size: 2771 1717
MluI A'CGCG_T
Cuts at: 0 176 4488
Size: 176 4312
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 1433 3220 3364 4488
Size: 713 720 1787 144 1124
Fragments arranged by size:
1787 1124 720 713 144
MscI TGG'CCA
Cuts at: 0 118 399 1304 4488
Size: 118 281 905 3184
Fragments arranged by size:
3184 905 281 118
MslI CAynn'nnrTG
Cuts at: 0 259 659 991 2156 4488
Size: 259 400 332 1165 2332
Fragments arranged by size:
2332 1165 400 332 259
MspA1I CmG'CkG
Cuts at: 0 492 768 1732 1884 2449 4120 4360 4372
Size: 492 276 964 152 565 1671 240 12
Cuts at: 4372 4488
Size: 116
Fragments arranged by size:
1671 964 565 492 276 240 152 116
12
NaeI GCC'GGC
Cuts at: 0 108 4488
Size: 108 4380
NarI GG'CG_CC
Cuts at: 0 33 1997 3667 3973 4488
Size: 33 1964 1670 306 515
Fragments arranged by size:
1964 1670 515 306 33
NcoI C'CATG_G
Cuts at: 0 3396 4488
Size: 3396 1092
NdeI CA'TA_TG
Cuts at: 0 2044 4488
Size: 2044 2444
NgoMIV G'CCGG_C
Cuts at: 0 106 4488
Size: 106 4382
NotI GC'GGCC_GC
Cuts at: 0 78 4488
Size: 78 4410
NspI r_CATG'y
Cuts at: 0 1500 4488
Size: 1500 2988
PasI CC'CwG_GG
Cuts at: 0 1233 4488
Size: 1233 3255
PflMI CCAn_nnn'nTGG
Cuts at: 0 406 2906 4222 4488
Size: 406 2500 1316 266
Fragments arranged by size:
2500 1316 406 266
PfoI T'CCnGG_A
Cuts at: 0 2633 2843 3548 4488
Size: 2633 210 705 940
Fragments arranged by size:
2633 940 705 210
PmlI CAC'GTG
Cuts at: 0 501 4488
Size: 501 3987
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 746 778 4488
Size: 746 32 3710
Fragments arranged by size:
3710 746 32
PpuMI rG'GwC_Cy
Cuts at: 0 1209 1604 2727 3603 4041 4236 4488
Size: 1209 395 1123 876 438 195 252
Fragments arranged by size:
1209 1123 876 438 395 252 195
PsiI TTA'TAA
Cuts at: 0 3488 4488
Size: 3488 1000
PspOMI G'GGCC_C
Cuts at: 0 2655 3796 4488
Size: 2655 1141 692
Fragments arranged by size:
2655 1141 692
PspXI vC'TCGA_Gb
Cuts at: 0 1204 3842 4488
Size: 1204 2638 646
Fragments arranged by size:
2638 1204 646
PstI C_TGCA'G
Cuts at: 0 1223 2528 3894 4040 4049 4463 4488
Size: 1223 1305 1366 146 9 414 25
Fragments arranged by size:
1366 1305 1223 414 146 25 9
PvuI CG_AT'CG
Cuts at: 0 2582 4488
Size: 2582 1906
PvuII CAG'CTG
Cuts at: 0 492 1732 1884 2449 4360 4372 4488
Size: 492 1240 152 565 1911 12 116
Fragments arranged by size:
1911 1240 565 492 152 116 12
SacI G_AGCT'C
Cuts at: 0 2744 3230 4300 4488
Size: 2744 486 1070 188
Fragments arranged by size:
2744 1070 486 188
SacII CC_GC'GG
Cuts at: 0 4121 4488
Size: 4121 367
SbfI CC_TGCA'GG
Cuts at: 0 4040 4488
Size: 4040 448
ScaI AGT'ACT
Cuts at: 0 1800 4488
Size: 1800 2688
SexAI A'CCwGG_T
Cuts at: 0 518 2506 3218 4488
Size: 518 1988 712 1270
Fragments arranged by size:
1988 1270 712 518
SfcI C'TryA_G
Cuts at: 0 54 1219 2266 2272 2524 3890 4036 4045
Size: 54 1165 1047 6 252 1366 146 9
Cuts at: 4045 4459 4488
Size: 414 29
Fragments arranged by size:
1366 1165 1047 414 252 146 54 29
9 6
SfoI GGC'GCC
Cuts at: 0 34 1998 3668 3974 4488
Size: 34 1964 1670 306 514
Fragments arranged by size:
1964 1670 514 306 34
SmlI C'TyrA_G
Cuts at: 0 507 1056 1204 1225 3229 3726 3842 4488
Size: 507 549 148 21 2004 497 116 646
Fragments arranged by size:
2004 646 549 507 497 148 116 21
SphI G_CATG'C
Cuts at: 0 1500 4488
Size: 1500 2988
SspI AAT'ATT
Cuts at: 0 1785 4488
Size: 1785 2703
StuI AGG'CCT
Cuts at: 0 607 827 4488
Size: 607 220 3661
Fragments arranged by size:
3661 607 220
StyI C'CwwG_G
Cuts at: 0 332 602 1355 2753 2793 2900 3396 3458
Size: 332 270 753 1398 40 107 496 62
Cuts at: 3458 3598 3989 4376 4488
Size: 140 391 387 112
Fragments arranged by size:
1398 753 496 391 387 332 270 140
112 107 62 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1187 2125 2602 2907 3279 3409 3711 4124
Size: 1187 938 477 305 372 130 302 413
Cuts at: 4124 4413 4488
Size: 289 75
Fragments arranged by size:
1187 938 477 413 372 305 302 289
130 75
TatI w'GTAC_w
Cuts at: 0 1798 4488
Size: 1798 2690
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 111 143 917 949 2496 2528 3938 3970
Size: 111 32 774 32 1547 32 1410 32
Cuts at: 3970 4488
Size: 518
Fragments arranged by size:
1547 1410 774 518 111 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 2466 2478 4488
Size: 2466 12 2010
Fragments arranged by size:
2466 2010 12
XhoI C'TCGA_G
Cuts at: 0 1204 3842 4488
Size: 1204 2638 646
Fragments arranged by size:
2638 1204 646
XmnI GAAnn'nnTTC
Cuts at: 0 475 1829 4488
Size: 475 1354 2659
Fragments arranged by size:
2659 1354 475
ZraI GAC'GTC
Cuts at: 0 1464 4488
Size: 1464 3024
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BclI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NspI
PasI PflMI PfoI PmlI PpiI PpuMI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SbfI ScaI
SexAI SfcI SfoI SmlI SphI SspI StuI StyI
TaqII TatI TstI XbaI XhoI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AloI ApaLI AscI AseI AsiSI
AvrII BaeI BciVI BfrBI BglII BmtI BsaBI BsmI
BsrDI BsrGI BssSI BstBI BstZ17I ClaI CspCI DraI
EcoRV FseI FspI FspAI HindIII HpaI NheI NruI
NsiI PacI PciI PmeI PshAI PsrI RsrII SalI
SanDI SapI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SwaI Tth111I XcmI XmaI