Restriction map for
pF1KA0350
(Linear) (Six-base) MAPSORT of: KIAA0350.seq Check: 8266 from: 1 to: 3159
KIAA0350.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 788 854 1417 2265 2558 3159
Size: 788 66 563 848 293 601
Fragments arranged by size:
848 788 601 563 293 66
AclI AA'CG_TT
Cuts at: 0 1184 3159
Size: 1184 1975
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 1726 2525 3159
Size: 1022 704 799 634
Fragments arranged by size:
1022 799 704 634
AfeI AGC'GCT
Cuts at: 0 2608 3159
Size: 2608 551
AflIII A'CryG_T
Cuts at: 0 275 875 1835 3159
Size: 275 600 960 1324
Fragments arranged by size:
1324 960 600 275
AleI CACnn'nnGTG
Cuts at: 0 1360 3159
Size: 1360 1799
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1378 2473 3159
Size: 1378 1095 686
Fragments arranged by size:
1378 1095 686
AlwNI CAG_nnn'CTG
Cuts at: 0 291 2421 2550 2747 3159
Size: 291 2130 129 197 412
Fragments arranged by size:
2130 412 291 197 129
ApaI G_GGCC'C
Cuts at: 0 1217 3159
Size: 1217 1942
ApaLI G'TGCA_C
Cuts at: 0 1750 3159
Size: 1750 1409
ApoI r'AATT_y
Cuts at: 0 369 388 3159
Size: 369 19 2771
Fragments arranged by size:
2771 369 19
AvaI C'yCGr_G
Cuts at: 0 1114 2072 3159
Size: 1114 958 1087
Fragments arranged by size:
1114 1087 958
BamHI G'GATC_C
Cuts at: 0 1542 3159
Size: 1542 1617
BanI G'GyrC_C
Cuts at: 0 2576 2634 2761 3145 3159
Size: 2576 58 127 384 14
Fragments arranged by size:
2576 384 127 58 14
BanII G_rGCy'C
Cuts at: 0 1217 2722 3110 3159
Size: 1217 1505 388 49
Fragments arranged by size:
1505 1217 388 49
BbeI G_GCGC'C
Cuts at: 0 2580 3159
Size: 2580 579
BbsI GAAGACnn'nnnn_
Cuts at: 0 951 1241 1600 1826 1990 3159
Size: 951 290 359 226 164 1169
Fragments arranged by size:
1169 951 359 290 226 164
BbvCI CC'TCA_GC
Cuts at: 0 3152 3159
Size: 3152 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 718 752 1932 1966 2113 2147 2284 2318
Size: 718 34 1180 34 147 34 137 34
Cuts at: 2318 3106 3140 3159
Size: 788 34 19
Fragments arranged by size:
1180 788 718 147 137 34 34 34
34 34 19
BclI T'GATC_A
Cuts at: 0 803 1316 2087 2144 3159
Size: 803 513 771 57 1015
Fragments arranged by size:
1015 803 771 513 57
BglI GCCn_nnn'nGGC
Cuts at: 0 1246 2726 3065 3159
Size: 1246 1480 339 94
Fragments arranged by size:
1480 1246 339 94
BlpI GC'TnA_GC
Cuts at: 0 1690 3004 3134 3159
Size: 1690 1314 130 25
Fragments arranged by size:
1690 1314 130 25
Bme1580I G_kGCm'C
Cuts at: 0 1217 1754 2307 3148 3159
Size: 1217 537 553 841 11
Fragments arranged by size:
1217 841 553 537 11
BmgBI CAC'GTC
Cuts at: 0 1749 3159
Size: 1749 1410
BmrI ACTGGGnnnn_n'
Cuts at: 0 2649 3028 3159
Size: 2649 379 131
Fragments arranged by size:
2649 379 131
BmtI G_CTAG'C
Cuts at: 0 1618 3159
Size: 1618 1541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 907 1705 1786 2350 2777 3159
Size: 238 669 798 81 564 427 382
Fragments arranged by size:
798 669 564 427 382 238 81
Bpu10I CC'TnA_GC
Cuts at: 0 2831 3152 3159
Size: 2831 321 7
Fragments arranged by size:
2831 321 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 1147 3159
Size: 61 1086 2012
Fragments arranged by size:
2012 1086 61
BsaI GGTCTCn'nnnn_
Cuts at: 0 139 325 1161 3159
Size: 139 186 836 1998
Fragments arranged by size:
1998 836 186 139
BsaAI yAC'GTr
Cuts at: 0 276 366 876 3159
Size: 276 90 510 2283
Fragments arranged by size:
2283 510 276 90
BsaBI GATnn'nnATC
Cuts at: 0 172 1315 3159
Size: 172 1143 1844
Fragments arranged by size:
1844 1143 172
BsaHI Gr'CG_yC
Cuts at: 0 1238 1901 2577 3159
Size: 1238 663 676 582
Fragments arranged by size:
1238 676 663 582
BsaWI w'CCGG_w
Cuts at: 0 128 3159
Size: 128 3031
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 46 76 3159
Size: 46 30 3083
Fragments arranged by size:
3083 46 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 421 739 920 1219 1402 1501 2092 2520
Size: 421 318 181 299 183 99 591 428
Cuts at: 2520 3159
Size: 639
Fragments arranged by size:
639 591 428 421 318 299 183 181
99
BseYI C'CCAG_C
Cuts at: 0 20 1078 1652 2234 2722 2890 2941 3159
Size: 20 1058 574 582 488 168 51 218
Fragments arranged by size:
1058 582 574 488 218 168 51 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 733 766 1384 2623 3084 3159
Size: 607 126 33 618 1239 461 75
Fragments arranged by size:
1239 618 607 461 126 75 33
BsiEI CG_ry'CG
Cuts at: 0 916 1589 3159
Size: 916 673 1570
Fragments arranged by size:
1570 916 673
BsiHKAI G_wGCw'C
Cuts at: 0 494 770 836 1423 1505 1754 2740 3159
Size: 494 276 66 587 82 249 986 419
Fragments arranged by size:
986 587 494 419 276 249 82 66
BsmI GAATG_Cn'
Cuts at: 0 1082 3159
Size: 1082 2077
Bsp1286I G_dGCh'C
Cuts at: 0 494 770 836 1217 1423 1505 1754 2307
Size: 494 276 66 381 206 82 249 553
Cuts at: 2307 2722 2740 3110 3148 3159
Size: 415 18 370 38 11
Fragments arranged by size:
553 494 415 381 370 276 249 206
82 66 38 18 11
BspHI T'CATG_A
Cuts at: 0 1637 1739 1892 3159
Size: 1637 102 153 1267
Fragments arranged by size:
1637 1267 153 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 143 788 854 1417 2265 2558 3159
Size: 143 645 66 563 848 293 601
Fragments arranged by size:
848 645 601 563 293 143 66
BsrBI CCG'CTC
Cuts at: 0 11 1119 3159
Size: 11 1108 2040
Fragments arranged by size:
2040 1108 11
BsrDI GCAATG_nn'
Cuts at: 0 1432 3159
Size: 1432 1727
BsrFI r'CCGG_y
Cuts at: 0 930 3064 3087 3159
Size: 930 2134 23 72
Fragments arranged by size:
2134 930 72 23
BsrGI T'GTAC_A
Cuts at: 0 512 2137 3159
Size: 512 1625 1022
Fragments arranged by size:
1625 1022 512
BssSI C'ACGA_G
Cuts at: 0 328 3159
Size: 328 2831
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1088 2271 3159
Size: 1088 1183 888
Fragments arranged by size:
1183 1088 888
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1660 1881 3159
Size: 1660 221 1278
Fragments arranged by size:
1660 1278 221
BstYI r'GATC_y
Cuts at: 0 167 1542 1667 2109 3159
Size: 167 1375 125 442 1050
Fragments arranged by size:
1375 1050 442 167 125
BtgI C'CryG_G
Cuts at: 0 36 1379 2648 2918 3110 3159
Size: 36 1343 1269 270 192 49
Fragments arranged by size:
1343 1269 270 192 49 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1988 2391 3159
Size: 1988 403 768
Fragments arranged by size:
1988 768 403
BtsI GCAGTG_nn'
Cuts at: 0 2029 2745 3159
Size: 2029 716 414
Fragments arranged by size:
2029 716 414
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1269 1304 3159
Size: 1269 35 1855
Fragments arranged by size:
1855 1269 35
EaeI y'GGCC_r
Cuts at: 0 6 913 1346 1586 2423 2903 3159
Size: 6 907 433 240 837 480 256
Fragments arranged by size:
907 837 480 433 256 240 6
EagI C'GGCC_G
Cuts at: 0 913 1586 3159
Size: 913 673 1573
Fragments arranged by size:
1573 913 673
EarI CTCTTCn'nnn_
Cuts at: 0 865 1295 3113 3159
Size: 865 430 1818 46
Fragments arranged by size:
1818 865 430 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2697 3159
Size: 2697 462
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 907 1022 1705 1726 1786 2350 2525
Size: 238 669 115 683 21 60 564 175
Cuts at: 2525 2777 3159
Size: 252 382
Fragments arranged by size:
683 669 564 382 252 238 175 115
60 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2263 3159
Size: 2263 896
EcoO109I rG'GnC_Cy
Cuts at: 0 1213 2082 3159
Size: 1213 869 1077
Fragments arranged by size:
1213 1077 869
HaeII r_GCGC'y
Cuts at: 0 1400 2421 2580 2610 3159
Size: 1400 1021 159 30 549
Fragments arranged by size:
1400 1021 549 159 30
HincII GTy'rAC
Cuts at: 0 2865 3039 3159
Size: 2865 174 120
Fragments arranged by size:
2865 174 120
KasI G'GCGC_C
Cuts at: 0 2576 3159
Size: 2576 583
MfeI C'AATT_G
Cuts at: 0 2035 3159
Size: 2035 1124
MscI TGG'CCA
Cuts at: 0 2425 2905 3159
Size: 2425 480 254
Fragments arranged by size:
2425 480 254
MslI CAynn'nnrTG
Cuts at: 0 497 1360 1864 1879 2002 2275 2455 3067
Size: 497 863 504 15 123 273 180 612
Cuts at: 3067 3159
Size: 92
Fragments arranged by size:
863 612 504 497 273 180 123 92
15
MspA1I CmG'CkG
Cuts at: 0 288 984 2067 2399 2714 2726 2732 3078
Size: 288 696 1083 332 315 12 6 346
Cuts at: 3078 3151 3159
Size: 73 8
Fragments arranged by size:
1083 696 346 332 315 288 73 12
8 6
NaeI GCC'GGC
Cuts at: 0 3089 3159
Size: 3089 70
NarI GG'CG_CC
Cuts at: 0 2577 3159
Size: 2577 582
NcoI C'CATG_G
Cuts at: 0 36 3159
Size: 36 3123
NgoMIV G'CCGG_C
Cuts at: 0 3087 3159
Size: 3087 72
NheI G'CTAG_C
Cuts at: 0 1614 3159
Size: 1614 1545
NspI r_CATG'y
Cuts at: 0 1839 2138 2274 3072 3131 3159
Size: 1839 299 136 798 59 28
Fragments arranged by size:
1839 798 299 136 59 28
PciI A'CATG_T
Cuts at: 0 1835 3159
Size: 1835 1324
PflMI CCAn_nnn'nTGG
Cuts at: 0 66 1464 3159
Size: 66 1398 1695
Fragments arranged by size:
1695 1398 66
PfoI T'CCnGG_A
Cuts at: 0 215 1442 2105 2477 3159
Size: 215 1227 663 372 682
Fragments arranged by size:
1227 682 663 372 215
PpuMI rG'GwC_Cy
Cuts at: 0 2082 3159
Size: 2082 1077
PshAI GACnn'nnGTC
Cuts at: 0 3025 3159
Size: 3025 134
PsiI TTA'TAA
Cuts at: 0 485 1811 3159
Size: 485 1326 1348
Fragments arranged by size:
1348 1326 485
PspOMI G'GGCC_C
Cuts at: 0 1213 3159
Size: 1213 1946
PspXI vC'TCGA_Gb
Cuts at: 0 1114 3159
Size: 1114 2045
PstI C_TGCA'G
Cuts at: 0 296 3159
Size: 296 2863
PvuII CAG'CTG
Cuts at: 0 288 3159
Size: 288 2871
SapI GCTCTTCn'nnn_
Cuts at: 0 865 3159
Size: 865 2294
SfcI C'TryA_G
Cuts at: 0 292 3159
Size: 292 2867
SfoI GGC'GCC
Cuts at: 0 2578 3159
Size: 2578 581
SmlI C'TyrA_G
Cuts at: 0 76 1114 1126 3159
Size: 76 1038 12 2033
Fragments arranged by size:
2033 1038 76 12
SnaBI TAC'GTA
Cuts at: 0 366 3159
Size: 366 2793
SphI G_CATG'C
Cuts at: 0 3072 3159
Size: 3072 87
StuI AGG'CCT
Cuts at: 0 2258 3159
Size: 2258 901
StyI C'CwwG_G
Cuts at: 0 36 65 2150 3159
Size: 36 29 2085 1009
Fragments arranged by size:
2085 1009 36 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 12 3159
Size: 12 3147
TatI w'GTAC_w
Cuts at: 0 512 878 2137 3159
Size: 512 366 1259 1022
Fragments arranged by size:
1259 1022 512 366
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2418 2508 2752 2898 3159
Size: 2418 90 244 146 261
Fragments arranged by size:
2418 261 244 146 90
XhoI C'TCGA_G
Cuts at: 0 1114 3159
Size: 1114 2045
XmnI GAAnn'nnTTC
Cuts at: 0 1007 1787 3159
Size: 1007 780 1372
Fragments arranged by size:
1372 1007 780
Enzymes that do cut:
AarI AclI AcuI AfeI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII
HincII KasI MfeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PflMI PfoI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuII SapI SfcI
SfoI SmlI SnaBI SphI StuI StyI TaqII TatI
XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AflII AgeI AhdI AloI AscI
AseI AsiSI AvrII BaeI BciVI BfrBI BglII BplI
BsiWI BsmBI BspEI BssHII BstBI BstEII BstZ17I Bsu36I
ClaI DraI DraIII DrdI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HindIII HpaI KpnI MluI
MmeI NdeI NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SpeI SrfI SspI SwaI TstI Tth111I XbaI XmaI
ZraI