Restriction map for
pF1KA0351
(Linear) (Six-base) MAPSORT of: KIAA0351.seq Check: 5415 from: 1 to: 1671
KIAA0351.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
Acc65I G'GTAC_C
Cuts at: 0 1644 1671
Size: 1644 27
AclI AA'CG_TT
Cuts at: 0 269 1671
Size: 269 1402
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 601 805 1217 1671
Size: 444 157 204 412 454
Fragments arranged by size:
454 444 412 204 157
AlwNI CAG_nnn'CTG
Cuts at: 0 1373 1671
Size: 1373 298
ApoI r'AATT_y
Cuts at: 0 323 1671
Size: 323 1348
AseI AT'TA_AT
Cuts at: 0 178 1671
Size: 178 1493
AvaI C'yCGr_G
Cuts at: 0 1510 1671
Size: 1510 161
AvrII C'CTAG_G
Cuts at: 0 1026 1191 1671
Size: 1026 165 480
Fragments arranged by size:
1026 480 165
BamHI G'GATC_C
Cuts at: 0 1390 1671
Size: 1390 281
BanI G'GyrC_C
Cuts at: 0 1100 1644 1671
Size: 1100 544 27
Fragments arranged by size:
1100 544 27
BanII G_rGCy'C
Cuts at: 0 483 812 1210 1671
Size: 483 329 398 461
Fragments arranged by size:
483 461 398 329
BbsI GAAGACnn'nnnn_
Cuts at: 0 120 833 1223 1671
Size: 120 713 390 448
Fragments arranged by size:
713 448 390 120
BbvCI CC'TCA_GC
Cuts at: 0 364 1671
Size: 364 1307
BclI T'GATC_A
Cuts at: 0 747 1671
Size: 747 924
BfrBI ATG'CAT
Cuts at: 0 1621 1671
Size: 1621 50
BglI GCCn_nnn'nGGC
Cuts at: 0 1253 1671
Size: 1253 418
BglII A'GATC_T
Cuts at: 0 900 1671
Size: 900 771
Bme1580I G_kGCm'C
Cuts at: 0 1295 1432 1671
Size: 1295 137 239
Fragments arranged by size:
1295 239 137
BmrI ACTGGGnnnn_n'
Cuts at: 0 1383 1671
Size: 1383 288
BmtI G_CTAG'C
Cuts at: 0 29 167 1671
Size: 29 138 1504
Fragments arranged by size:
1504 138 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 796 980 1671
Size: 100 696 184 691
Fragments arranged by size:
696 691 184 100
Bpu10I CC'TnA_GC
Cuts at: 0 364 1671
Size: 364 1307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1315 1671
Size: 1315 356
BsaWI w'CCGG_w
Cuts at: 0 925 1671
Size: 925 746
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 1152 1155 1671
Size: 169 983 3 516
Fragments arranged by size:
983 516 169 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1513 1671
Size: 793 720 158
Fragments arranged by size:
793 720 158
BsiHKAI G_wGCw'C
Cuts at: 0 453 812 1210 1517 1671
Size: 453 359 398 307 154
Fragments arranged by size:
453 398 359 307 154
BsiWI C'GTAC_G
Cuts at: 0 792 1671
Size: 792 879
Bsp1286I G_dGCh'C
Cuts at: 0 453 483 812 1210 1295 1432 1517 1671
Size: 453 30 329 398 85 137 85 154
Fragments arranged by size:
453 398 329 154 137 85 85 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 898 1656 1671
Size: 898 758 15
Fragments arranged by size:
898 758 15
BsrDI GCAATG_nn'
Cuts at: 0 1560 1671
Size: 1560 111
BsrFI r'CCGG_y
Cuts at: 0 1252 1671
Size: 1252 419
BsrGI T'GTAC_A
Cuts at: 0 2 1671
Size: 2 1669
BssSI C'ACGA_G
Cuts at: 0 1359 1671
Size: 1359 312
BstAPI GCAn_nnn'nTGC
Cuts at: 0 652 1671
Size: 652 1019
BstBI TT'CG_AA
Cuts at: 0 711 1671
Size: 711 960
BstEII G'GTnAC_C
Cuts at: 0 36 1671
Size: 36 1635
BstYI r'GATC_y
Cuts at: 0 685 900 1390 1671
Size: 685 215 490 281
Fragments arranged by size:
685 490 281 215
Bsu36I CC'TnA_GG
Cuts at: 0 657 1640 1671
Size: 657 983 31
Fragments arranged by size:
983 657 31
BtgI C'CryG_G
Cuts at: 0 1298 1671
Size: 1298 373
DraI TTT'AAA
Cuts at: 0 198 344 488 1671
Size: 198 146 144 1183
Fragments arranged by size:
1183 198 146 144
DrdI GACnn_nn'nnGTC
Cuts at: 0 1117 1671
Size: 1117 554
EarI CTCTTCn'nnn_
Cuts at: 0 800 894 1009 1217 1671
Size: 800 94 115 208 454
Fragments arranged by size:
800 454 208 115 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1356 1671
Size: 1356 315
EcoICRI GAG'CTC
Cuts at: 0 810 1208 1671
Size: 810 398 463
Fragments arranged by size:
810 463 398
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 444 601 796 805 980 1217 1671
Size: 100 344 157 195 9 175 237 454
Fragments arranged by size:
454 344 237 195 175 157 100 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 656 1330 1547 1671
Size: 656 674 217 124
Fragments arranged by size:
674 656 217 124
EcoO109I rG'GnC_Cy
Cuts at: 0 910 1146 1306 1671
Size: 910 236 160 365
Fragments arranged by size:
910 365 236 160
EcoRV GAT'ATC
Cuts at: 0 1524 1671
Size: 1524 147
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 525 1671
Size: 525 1146
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 32 1382 1414 1671
Size: 32 1350 32 257
Fragments arranged by size:
1350 257 32 32
KpnI G_GTAC'C
Cuts at: 0 1648 1671
Size: 1648 23
MslI CAynn'nnrTG
Cuts at: 0 427 1613 1671
Size: 427 1186 58
Fragments arranged by size:
1186 427 58
MspA1I CmG'CkG
Cuts at: 0 227 1288 1497 1533 1671
Size: 227 1061 209 36 138
Fragments arranged by size:
1061 227 209 138 36
NcoI C'CATG_G
Cuts at: 0 1298 1671
Size: 1298 373
NheI G'CTAG_C
Cuts at: 0 25 163 1671
Size: 25 138 1508
Fragments arranged by size:
1508 138 25
NsiI A_TGCA'T
Cuts at: 0 1623 1671
Size: 1623 48
NspI r_CATG'y
Cuts at: 0 1625 1671
Size: 1625 46
PflMI CCAn_nnn'nTGG
Cuts at: 0 1373 1671
Size: 1373 298
PpuMI rG'GwC_Cy
Cuts at: 0 910 1671
Size: 910 761
PstI C_TGCA'G
Cuts at: 0 742 1671
Size: 742 929
PvuII CAG'CTG
Cuts at: 0 227 1288 1497 1533 1671
Size: 227 1061 209 36 138
Fragments arranged by size:
1061 227 209 138 36
SacI G_AGCT'C
Cuts at: 0 812 1210 1671
Size: 812 398 461
Fragments arranged by size:
812 461 398
SapI GCTCTTCn'nnn_
Cuts at: 0 800 1009 1671
Size: 800 209 662
Fragments arranged by size:
800 662 209
SexAI A'CCwGG_T
Cuts at: 0 296 1366 1671
Size: 296 1070 305
Fragments arranged by size:
1070 305 296
SfcI C'TryA_G
Cuts at: 0 738 1671
Size: 738 933
SmlI C'TyrA_G
Cuts at: 0 1330 1671
Size: 1330 341
SpeI A'CTAG_T
Cuts at: 0 882 1671
Size: 882 789
SspI AAT'ATT
Cuts at: 0 705 1671
Size: 705 966
StuI AGG'CCT
Cuts at: 0 1638 1671
Size: 1638 33
StyI C'CwwG_G
Cuts at: 0 893 1026 1191 1298 1671
Size: 893 133 165 107 373
Fragments arranged by size:
893 373 165 133 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1532 1671
Size: 1532 139
TatI w'GTAC_w
Cuts at: 0 2 1403 1671
Size: 2 1401 268
Fragments arranged by size:
1401 268 2
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 983 1015 1435 1467 1671
Size: 983 32 420 32 204
Fragments arranged by size:
983 420 204 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1117 1671
Size: 1117 554
XbaI T'CTAG_A
Cuts at: 0 396 1671
Size: 396 1275
Enzymes that do cut:
Acc65I AclI AcuI AlwNI ApoI AseI AvaI AvrII
BamHI BanI BanII BbsI BbvCI BclI BfrBI BglI
BglII Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaWI
BseRI BsgI BsiHKAI BsiWI Bsp1286I BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
DraI DrdI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI Hin4I KpnI MslI MspA1I NcoI NheI
NsiI NspI PflMI PpuMI PstI PvuII SacI SapI
SexAI SfcI SmlI SpeI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI BaeI
BbeI BcgI BciVI BlpI BmgBI BplI BsaI BsaAI
BsaBI BsaHI BsaXI BseYI BsiEI BsmI BsmBI BspEI
BspHI BsrBI BssHII BstXI BstZ17I BtgZI BtsI ClaI
CspCI DraIII EaeI EagI EcoRI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI MfeI MluI MmeI
MscI NaeI NarI NdeI NgoMIV NotI NruI PacI
PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SwaI XcmI XhoI XmaI XmnI ZraI