Restriction map for
pF1KA0354
(Linear) (Six-base) MAPSORT of: KIAA0354.seq Check: 8781 from: 1 to: 2031
KIAA0354.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 323 1354 2031
Size: 323 1031 677
Fragments arranged by size:
1031 677 323
AccI GT'mk_AC
Cuts at: 0 253 2031
Size: 253 1778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 1463 1951 2031
Size: 653 810 488 80
Fragments arranged by size:
810 653 488 80
AfeI AGC'GCT
Cuts at: 0 424 2031
Size: 424 1607
AleI CACnn'nnGTG
Cuts at: 0 1895 1907 2031
Size: 1895 12 124
Fragments arranged by size:
1895 124 12
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 14 46 2031
Size: 14 32 1985
Fragments arranged by size:
1985 32 14
AlwNI CAG_nnn'CTG
Cuts at: 0 39 444 1354 2031
Size: 39 405 910 677
Fragments arranged by size:
910 677 405 39
ApaI G_GGCC'C
Cuts at: 0 1373 2031
Size: 1373 658
ApoI r'AATT_y
Cuts at: 0 1230 2031
Size: 1230 801
AvaI C'yCGr_G
Cuts at: 0 683 2031
Size: 683 1348
BanI G'GyrC_C
Cuts at: 0 813 2031
Size: 813 1218
BanII G_rGCy'C
Cuts at: 0 153 466 517 1006 1373 1651 1830 2031
Size: 153 313 51 489 367 278 179 201
Fragments arranged by size:
489 367 313 278 201 179 153 51
BbvCI CC'TCA_GC
Cuts at: 0 1108 2031
Size: 1108 923
BclI T'GATC_A
Cuts at: 0 780 897 2031
Size: 780 117 1134
Fragments arranged by size:
1134 780 117
BglI GCCn_nnn'nGGC
Cuts at: 0 488 2031
Size: 488 1543
BlpI GC'TnA_GC
Cuts at: 0 465 2031
Size: 465 1566
Bme1580I G_kGCm'C
Cuts at: 0 818 1373 2031
Size: 818 555 658
Fragments arranged by size:
818 658 555
BmrI ACTGGGnnnn_n'
Cuts at: 0 380 1140 2031
Size: 380 760 891
Fragments arranged by size:
891 760 380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1339 1536 2031
Size: 1339 197 495
Fragments arranged by size:
1339 495 197
Bpu10I CC'TnA_GC
Cuts at: 0 1010 1108 2031
Size: 1010 98 923
Fragments arranged by size:
1010 923 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 2031
Size: 782 1249
BsaHI Gr'CG_yC
Cuts at: 0 564 2031
Size: 564 1467
BsaWI w'CCGG_w
Cuts at: 0 654 2031
Size: 654 1377
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1070 1100 2031
Size: 1070 30 931
Fragments arranged by size:
1070 931 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 508 607 703 1365 1508 1533 1595 2031
Size: 508 99 96 662 143 25 62 436
Fragments arranged by size:
662 508 436 143 99 96 62 25
BseYI C'CCAG_C
Cuts at: 0 270 444 817 1354 1730 2017 2031
Size: 270 174 373 537 376 287 14
Fragments arranged by size:
537 376 373 287 270 174 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 893 1492 2031
Size: 218 675 599 539
Fragments arranged by size:
675 599 539 218
BsiHKAI G_wGCw'C
Cuts at: 0 466 517 971 1006 1651 1805 1830 2031
Size: 466 51 454 35 645 154 25 201
Fragments arranged by size:
645 466 454 201 154 51 35 25
BsmBI CGTCTCn'nnnn_
Cuts at: 0 555 2031
Size: 555 1476
Bsp1286I G_dGCh'C
Cuts at: 0 153 466 517 818 971 1006 1373 1651
Size: 153 313 51 301 153 35 367 278
Cuts at: 1651 1805 1830 2031
Size: 154 25 201
Fragments arranged by size:
367 313 301 278 201 154 153 153
51 35 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 323 1354 1425 1996 2031
Size: 323 1031 71 571 35
Fragments arranged by size:
1031 571 323 71 35
BsrBI CCG'CTC
Cuts at: 0 116 597 2031
Size: 116 481 1434
Fragments arranged by size:
1434 481 116
BsrDI GCAATG_nn'
Cuts at: 0 288 2031
Size: 288 1743
BssSI C'ACGA_G
Cuts at: 0 869 2031
Size: 869 1162
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1850 2031
Size: 1850 181
BstBI TT'CG_AA
Cuts at: 0 1228 2031
Size: 1228 803
BstYI r'GATC_y
Cuts at: 0 1140 1655 2031
Size: 1140 515 376
Fragments arranged by size:
1140 515 376
BstZ17I GTA'TAC
Cuts at: 0 254 2031
Size: 254 1777
Bsu36I CC'TnA_GG
Cuts at: 0 944 1016 2031
Size: 944 72 1015
Fragments arranged by size:
1015 944 72
BtgI C'CryG_G
Cuts at: 0 860 2031
Size: 860 1171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1046 2031
Size: 1046 985
BtsI GCAGTG_nn'
Cuts at: 0 1128 2031
Size: 1128 903
DraI TTT'AAA
Cuts at: 0 105 2031
Size: 105 1926
EaeI y'GGCC_r
Cuts at: 0 60 1719 2031
Size: 60 1659 312
Fragments arranged by size:
1659 312 60
EarI CTCTTCn'nnn_
Cuts at: 0 522 2031
Size: 522 1509
EcoICRI GAG'CTC
Cuts at: 0 464 515 1004 1649 1828 2031
Size: 464 51 489 645 179 203
Fragments arranged by size:
645 489 464 203 179 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 1339 1463 1536 1951 2031
Size: 653 686 124 73 415 80
Fragments arranged by size:
686 653 415 124 80 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1551 2031
Size: 1551 480
EcoO109I rG'GnC_Cy
Cuts at: 0 1329 1370 2031
Size: 1329 41 661
Fragments arranged by size:
1329 661 41
EcoRV GAT'ATC
Cuts at: 0 1173 2031
Size: 1173 858
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1809 2031
Size: 1809 222
FspI TGC'GCA
Cuts at: 0 526 2031
Size: 526 1505
HaeII r_GCGC'y
Cuts at: 0 413 426 612 2031
Size: 413 13 186 1419
Fragments arranged by size:
1419 413 186 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 440 472 1024 1056 1070 1102 1132 1164
Size: 440 32 552 32 14 32 30 32
Cuts at: 1164 1195 1227 1759 1791 1935 1967 1995 2027
Size: 31 32 532 32 144 32 28 32
Cuts at: 2027 2031
Size: 4
Fragments arranged by size:
552 532 440 144 32 32 32 32
32 32 32 32 31 30 28 14
4
HincII GTy'rAC
Cuts at: 0 1751 2031
Size: 1751 280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 639 2031
Size: 639 1392
MscI TGG'CCA
Cuts at: 0 62 1721 2031
Size: 62 1659 310
Fragments arranged by size:
1659 310 62
MslI CAynn'nnrTG
Cuts at: 0 85 187 265 976 1895 1907 2031
Size: 85 102 78 711 919 12 124
Fragments arranged by size:
919 711 124 102 85 78 12
MspA1I CmG'CkG
Cuts at: 0 39 198 444 2031
Size: 39 159 246 1587
Fragments arranged by size:
1587 246 159 39
NcoI C'CATG_G
Cuts at: 0 860 2031
Size: 860 1171
NspI r_CATG'y
Cuts at: 0 134 344 420 2031
Size: 134 210 76 1611
Fragments arranged by size:
1611 210 134 76
PflMI CCAn_nnn'nTGG
Cuts at: 0 1182 1354 2031
Size: 1182 172 677
Fragments arranged by size:
1182 677 172
PspOMI G'GGCC_C
Cuts at: 0 1369 2031
Size: 1369 662
PstI C_TGCA'G
Cuts at: 0 591 1315 1365 1422 1767 2031
Size: 591 724 50 57 345 264
Fragments arranged by size:
724 591 345 264 57 50
PvuII CAG'CTG
Cuts at: 0 39 198 444 2031
Size: 39 159 246 1587
Fragments arranged by size:
1587 246 159 39
SacI G_AGCT'C
Cuts at: 0 466 517 1006 1651 1830 2031
Size: 466 51 489 645 179 201
Fragments arranged by size:
645 489 466 201 179 51
SapI GCTCTTCn'nnn_
Cuts at: 0 522 2031
Size: 522 1509
SbfI CC_TGCA'GG
Cuts at: 0 1365 2031
Size: 1365 666
SfcI C'TryA_G
Cuts at: 0 436 587 1311 1361 1418 1763 2031
Size: 436 151 724 50 57 345 268
Fragments arranged by size:
724 436 345 268 151 57 50
SmlI C'TyrA_G
Cuts at: 0 797 2031
Size: 797 1234
SpeI A'CTAG_T
Cuts at: 0 889 1303 2031
Size: 889 414 728
Fragments arranged by size:
889 728 414
SphI G_CATG'C
Cuts at: 0 134 420 2031
Size: 134 286 1611
Fragments arranged by size:
1611 286 134
SspI AAT'ATT
Cuts at: 0 1290 2031
Size: 1290 741
StuI AGG'CCT
Cuts at: 0 226 1440 1792 2031
Size: 226 1214 352 239
Fragments arranged by size:
1214 352 239 226
StyI C'CwwG_G
Cuts at: 0 860 954 1488 1573 2031
Size: 860 94 534 85 458
Fragments arranged by size:
860 534 458 94 85
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 55 267 1527 2031
Size: 55 212 1260 504
Fragments arranged by size:
1260 504 212 55
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AloI AlwNI ApaI
ApoI AvaI BanI BanII BbvCI BclI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrDI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI FspI HaeII Hin4I HincII MmeI
MscI MslI MspA1I NcoI NspI PflMI PspOMI PstI
PvuII SacI SapI SbfI SfcI SmlI SpeI SphI
SspI StuI StyI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AlfI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BcgI BciVI BfrBI BglII BmgBI BmtI BplI
BsaI BsaAI BsaBI BsiEI BsiWI BsmI BspEI BspHI
BsrFI BsrGI BssHII BstEII BstXI ClaI CspCI DraIII
DrdI EagI EciI EcoRI FseI FspAI HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SrfI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI