Restriction map for
pF1KSDA0355
(Linear) (Six-base) MAPSORT of: KIAA0355.seq Check: 897 from: 1 to: 3210
KIAA0355.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 683 1072 1199 1568 3210
Size: 285 398 389 127 369 1642
Fragments arranged by size:
1642 398 389 369 285 127
AflII C'TTAA_G
Cuts at: 0 1096 3210
Size: 1096 2114
AhdI GACnn_n'nnGTC
Cuts at: 0 1427 3210
Size: 1427 1783
AleI CACnn'nnGTG
Cuts at: 0 1453 2834 2884 3210
Size: 1453 1381 50 326
Fragments arranged by size:
1453 1381 326 50
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 201 2033 3210
Size: 201 1832 1177
Fragments arranged by size:
1832 1177 201
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 743 775 2758 2790 3210
Size: 743 32 1983 32 420
Fragments arranged by size:
1983 743 420 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1242 2794 3042 3210
Size: 1242 1552 248 168
Fragments arranged by size:
1552 1242 248 168
ApoI r'AATT_y
Cuts at: 0 453 472 1824 3210
Size: 453 19 1352 1386
Fragments arranged by size:
1386 1352 453 19
AvaI C'yCGr_G
Cuts at: 0 1959 2249 2618 2668 2736 3210
Size: 1959 290 369 50 68 474
Fragments arranged by size:
1959 474 369 290 68 50
BanI G'GyrC_C
Cuts at: 0 1216 1361 2103 2151 2253 3210
Size: 1216 145 742 48 102 957
Fragments arranged by size:
1216 957 742 145 102 48
BanII G_rGCy'C
Cuts at: 0 1016 1993 2527 3210
Size: 1016 977 534 683
Fragments arranged by size:
1016 977 683 534
BbsI GAAGACnn'nnnn_
Cuts at: 0 258 1379 1426 1606 3210
Size: 258 1121 47 180 1604
Fragments arranged by size:
1604 1121 258 180 47
BbvCI CC'TCA_GC
Cuts at: 0 593 701 2156 2754 3210
Size: 593 108 1455 598 456
Fragments arranged by size:
1455 598 593 456 108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1202 1236 3061 3095 3210
Size: 1202 34 1825 34 115
Fragments arranged by size:
1825 1202 115 34 34
BclI T'GATC_A
Cuts at: 0 1926 3210
Size: 1926 1284
BglI GCCn_nnn'nGGC
Cuts at: 0 2609 3210
Size: 2609 601
BglII A'GATC_T
Cuts at: 0 294 1529 3210
Size: 294 1235 1681
Fragments arranged by size:
1681 1235 294
Bme1580I G_kGCm'C
Cuts at: 0 18 2090 2106 2121 2256 3210
Size: 18 2072 16 15 135 954
Fragments arranged by size:
2072 954 135 18 16 15
BmgBI CAC'GTC
Cuts at: 0 1440 3210
Size: 1440 1770
BmrI ACTGGGnnnn_n'
Cuts at: 0 3046 3198 3210
Size: 3046 152 12
Fragments arranged by size:
3046 152 12
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2769 3210
Size: 2769 441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 1494 1638 2197 3210
Size: 466 1028 144 559 1013
Fragments arranged by size:
1028 1013 559 466 144
Bpu10I CC'TnA_GC
Cuts at: 0 104 593 701 797 870 2156 2717 2754
Size: 104 489 108 96 73 1286 561 37
Cuts at: 2754 3210
Size: 456
Fragments arranged by size:
1286 561 489 456 108 104 96 73
37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2171 3210
Size: 2171 1039
BsaHI Gr'CG_yC
Cuts at: 0 1058 3210
Size: 1058 2152
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2195 2225 2858 2888 3210
Size: 2195 30 633 30 322
Fragments arranged by size:
2195 633 322 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2309 2866 3210
Size: 2309 557 344
Fragments arranged by size:
2309 557 344
BseYI C'CCAG_C
Cuts at: 0 108 442 965 2113 2138 2243 2282 2490
Size: 108 334 523 1148 25 105 39 208
Cuts at: 2490 2591 2962 2974 2980 3113 3210
Size: 101 371 12 6 133 97
Fragments arranged by size:
1148 523 371 334 208 133 108 105
101 97 39 25 12 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 379 806 1051 1255 2680 3121 3210
Size: 97 282 427 245 204 1425 441 89
Fragments arranged by size:
1425 441 427 282 245 204 97 89
BsiEI CG_ry'CG
Cuts at: 0 1079 2601 3210
Size: 1079 1522 609
Fragments arranged by size:
1522 1079 609
BsiHKAI G_wGCw'C
Cuts at: 0 120 129 803 1016 1445 2718 3210
Size: 120 9 674 213 429 1273 492
Fragments arranged by size:
1273 674 492 429 213 120 9
BsmI GAATG_Cn'
Cuts at: 0 2492 3210
Size: 2492 718
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1065 3210
Size: 1065 2145
Bsp1286I G_dGCh'C
Cuts at: 0 18 120 129 803 1016 1445 1993 2090
Size: 18 102 9 674 213 429 548 97
Cuts at: 2090 2106 2121 2256 2527 2718 3210
Size: 16 15 135 271 191 492
Fragments arranged by size:
674 548 492 429 271 213 191 135
102 97 18 16 15 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1565 2299 3210
Size: 1565 734 911
Fragments arranged by size:
1565 911 734
BsrDI GCAATG_nn'
Cuts at: 0 896 3210
Size: 896 2314
BsrFI r'CCGG_y
Cuts at: 0 570 898 2603 3210
Size: 570 328 1705 607
Fragments arranged by size:
1705 607 570 328
BsrGI T'GTAC_A
Cuts at: 0 2363 3210
Size: 2363 847
BstAPI GCAn_nnn'nTGC
Cuts at: 0 75 124 1002 3042 3210
Size: 75 49 878 2040 168
Fragments arranged by size:
2040 878 168 75 49
BstXI CCAn_nnnn'nTGG
Cuts at: 0 249 1358 2031 2221 2473 3210
Size: 249 1109 673 190 252 737
Fragments arranged by size:
1109 737 673 252 249 190
BstYI r'GATC_y
Cuts at: 0 294 1511 1529 3210
Size: 294 1217 18 1681
Fragments arranged by size:
1681 1217 294 18
BtgI C'CryG_G
Cuts at: 0 140 2030 2098 2882 3210
Size: 140 1890 68 784 328
Fragments arranged by size:
1890 784 328 140 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 519 2584 3210
Size: 519 2065 626
Fragments arranged by size:
2065 626 519
BtsI GCAGTG_nn'
Cuts at: 0 18 149 633 1012 2077 2525 2841 3084
Size: 18 131 484 379 1065 448 316 243
Cuts at: 3084 3210
Size: 126
Fragments arranged by size:
1065 484 448 379 316 243 131 126
18
DraIII CAC_nnn'GTG
Cuts at: 0 124 1361 3210
Size: 124 1237 1849
Fragments arranged by size:
1849 1237 124
EaeI y'GGCC_r
Cuts at: 0 143 398 1076 1248 1841 2605 2629 2923
Size: 143 255 678 172 593 764 24 294
Cuts at: 2923 3210
Size: 287
Fragments arranged by size:
764 678 593 294 287 255 172 143
24
EagI C'GGCC_G
Cuts at: 0 1076 3210
Size: 1076 2134
EarI CTCTTCn'nnn_
Cuts at: 0 304 1718 3210
Size: 304 1414 1492
Fragments arranged by size:
1492 1414 304
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 973 993 3210
Size: 973 20 2217
Fragments arranged by size:
2217 973 20
EcoICRI GAG'CTC
Cuts at: 0 1014 3210
Size: 1014 2196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 466 683 1072 1199 1494 1568 1638
Size: 285 181 217 389 127 295 74 70
Cuts at: 1638 2197 3210
Size: 559 1013
Fragments arranged by size:
1013 559 389 295 285 217 181 127
74 70
EcoO109I rG'GnC_Cy
Cuts at: 0 2434 2791 3210
Size: 2434 357 419
Fragments arranged by size:
2434 419 357
EcoRI G'AATT_C
Cuts at: 0 472 1824 3210
Size: 472 1352 1386
Fragments arranged by size:
1386 1352 472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 63 3210
Size: 63 3147
HaeII r_GCGC'y
Cuts at: 0 48 3210
Size: 48 3162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 694 726 1413 1445 1942 1974 2734 2766
Size: 694 32 687 32 497 32 760 32
Cuts at: 2766 3210
Size: 444
Fragments arranged by size:
760 694 687 497 444 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1272 3210
Size: 1272 1938
HindIII A'AGCT_T
Cuts at: 0 867 1493 1637 3210
Size: 867 626 144 1573
Fragments arranged by size:
1573 867 626 144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2283 3210
Size: 2283 927
MscI TGG'CCA
Cuts at: 0 1250 1843 2925 3210
Size: 1250 593 1082 285
Fragments arranged by size:
1250 1082 593 285
MslI CAynn'nnrTG
Cuts at: 0 493 1356 1453 1886 2029 2834 2884 3037
Size: 493 863 97 433 143 805 50 153
Cuts at: 3037 3210
Size: 173
Fragments arranged by size:
863 805 493 433 173 153 143 97
50
MspA1I CmG'CkG
Cuts at: 0 175 185 623 1119 1239 1453 1472 1518
Size: 175 10 438 496 120 214 19 46
Cuts at: 1518 3085 3210
Size: 1567 125
Fragments arranged by size:
1567 496 438 214 175 125 120 46
19 10
NaeI GCC'GGC
Cuts at: 0 900 2605 3210
Size: 900 1705 605
Fragments arranged by size:
1705 900 605
NcoI C'CATG_G
Cuts at: 0 2030 3210
Size: 2030 1180
NgoMIV G'CCGG_C
Cuts at: 0 898 2603 3210
Size: 898 1705 607
Fragments arranged by size:
1705 898 607
NspI r_CATG'y
Cuts at: 0 370 3210
Size: 370 2840
PasI CC'CwG_GG
Cuts at: 0 159 1425 3169 3210
Size: 159 1266 1744 41
Fragments arranged by size:
1744 1266 159 41
PflMI CCAn_nnn'nTGG
Cuts at: 0 573 2921 3210
Size: 573 2348 289
Fragments arranged by size:
2348 573 289
PfoI T'CCnGG_A
Cuts at: 0 482 3210
Size: 482 2728
PpuMI rG'GwC_Cy
Cuts at: 0 2434 3210
Size: 2434 776
PspXI vC'TCGA_Gb
Cuts at: 0 2736 3210
Size: 2736 474
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 310 342 3210
Size: 310 32 2868
Fragments arranged by size:
2868 310 32
PstI C_TGCA'G
Cuts at: 0 13 180 952 1400 1598 2520 3047 3079
Size: 13 167 772 448 198 922 527 32
Cuts at: 3079 3210
Size: 131
Fragments arranged by size:
922 772 527 448 198 167 131 32
13
PvuII CAG'CTG
Cuts at: 0 185 1119 1239 1453 1518 3210
Size: 185 934 120 214 65 1692
Fragments arranged by size:
1692 934 214 185 120 65
SacI G_AGCT'C
Cuts at: 0 1016 3210
Size: 1016 2194
ScaI AGT'ACT
Cuts at: 0 282 3210
Size: 282 2928
SexAI A'CCwGG_T
Cuts at: 0 1190 3210
Size: 1190 2020
SfcI C'TryA_G
Cuts at: 0 9 176 948 1396 1594 1799 2516 3043
Size: 9 167 772 448 198 205 717 527
Cuts at: 3043 3075 3210
Size: 32 135
Fragments arranged by size:
772 717 527 448 205 198 167 135
32 9
SmaI CCC'GGG
Cuts at: 0 2620 3210
Size: 2620 590
SmlI C'TyrA_G
Cuts at: 0 1096 2186 2736 3210
Size: 1096 1090 550 474
Fragments arranged by size:
1096 1090 550 474
SspI AAT'ATT
Cuts at: 0 1749 3210
Size: 1749 1461
StuI AGG'CCT
Cuts at: 0 3163 3210
Size: 3163 47
StyI C'CwwG_G
Cuts at: 0 2030 3149 3210
Size: 2030 1119 61
Fragments arranged by size:
2030 1119 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2943 3162 3210
Size: 2943 219 48
Fragments arranged by size:
2943 219 48
TatI w'GTAC_w
Cuts at: 0 280 2363 3210
Size: 280 2083 847
Fragments arranged by size:
2083 847 280
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1250 1282 2858 2890 3210
Size: 1250 32 1576 32 320
Fragments arranged by size:
1576 1250 320 32 32
XbaI T'CTAG_A
Cuts at: 0 712 1330 3210
Size: 712 618 1880
Fragments arranged by size:
1880 712 618
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 156 426 1836 3210
Size: 156 270 1410 1374
Fragments arranged by size:
1410 1374 270 156
XhoI C'TCGA_G
Cuts at: 0 2736 3210
Size: 2736 474
XmaI C'CCGG_G
Cuts at: 0 2618 3210
Size: 2618 592
XmnI GAAnn'nnTTC
Cuts at: 0 265 472 1603 3210
Size: 265 207 1131 1607
Fragments arranged by size:
1607 1131 265 207
Enzymes that do cut:
AcuI AflII AhdI AleI AlfI AloI AlwNI ApoI
AvaI BanI BanII BbsI BbvCI BcgI BclI BglI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstXI
BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PasI PflMI PfoI PpuMI PspXI PsrI
PstI PvuII SacI ScaI SexAI SfcI SmaI SmlI
SspI StuI StyI TaqII TatI TstI XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BciVI BfrBI BlpI BmtI BsaI BsaAI BsaBI
BsaWI BsiWI BspEI BspHI BsrBI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoNI
EcoRV FseI FspI FspAI HpaI KasI KpnI MfeI
MluI NarI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PvuI
RsrII SacII SalI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I
ZraI