Restriction map for
pF1KA0356
(Linear) (Six-base) MAPSORT of: KIAA0356.seq Check: 6454 from: 1 to: 3168
KIAA0356.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1071 3168
Size: 1071 2097
AccI GT'mk_AC
Cuts at: 0 2963 3168
Size: 2963 205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 662 707 1297 1361 2284 2680 2794
Size: 424 238 45 590 64 923 396 114
Cuts at: 2794 2839 3168
Size: 45 329
Fragments arranged by size:
923 590 424 396 329 238 114 64
45 45
AflIII A'CryG_T
Cuts at: 0 98 2714 3168
Size: 98 2616 454
Fragments arranged by size:
2616 454 98
AgeI A'CCGG_T
Cuts at: 0 908 3168
Size: 908 2260
AhdI GACnn_n'nnGTC
Cuts at: 0 2022 3168
Size: 2022 1146
AleI CACnn'nnGTG
Cuts at: 0 340 1715 1717 3168
Size: 340 1375 2 1451
Fragments arranged by size:
1451 1375 340 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1063 3168
Size: 1063 2105
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 469 501 3168
Size: 469 32 2667
Fragments arranged by size:
2667 469 32
AlwNI CAG_nnn'CTG
Cuts at: 0 685 738 1093 2090 2442 2658 3168
Size: 685 53 355 997 352 216 510
Fragments arranged by size:
997 685 510 355 352 216 53
ApaI G_GGCC'C
Cuts at: 0 2227 2685 3168
Size: 2227 458 483
Fragments arranged by size:
2227 483 458
ApaLI G'TGCA_C
Cuts at: 0 2979 3168
Size: 2979 189
ApoI r'AATT_y
Cuts at: 0 934 2404 2944 3168
Size: 934 1470 540 224
Fragments arranged by size:
1470 934 540 224
AvaI C'yCGr_G
Cuts at: 0 708 1352 1942 2414 3168
Size: 708 644 590 472 754
Fragments arranged by size:
754 708 644 590 472
BamHI G'GATC_C
Cuts at: 0 70 3168
Size: 70 3098
BanI G'GyrC_C
Cuts at: 0 1059 1137 1615 1947 2812 3168
Size: 1059 78 478 332 865 356
Fragments arranged by size:
1059 865 478 356 332 78
BanII G_rGCy'C
Cuts at: 0 860 1073 1658 1670 1991 2227 2685 2828
Size: 860 213 585 12 321 236 458 143
Cuts at: 2828 3168
Size: 340
Fragments arranged by size:
860 585 458 340 321 236 213 143
12
BbeI G_GCGC'C
Cuts at: 0 1141 1951 2816 3168
Size: 1141 810 865 352
Fragments arranged by size:
1141 865 810 352
BbsI GAAGACnn'nnnn_
Cuts at: 0 134 255 695 1782 3072 3168
Size: 134 121 440 1087 1290 96
Fragments arranged by size:
1290 1087 440 134 121 96
BbvCI CC'TCA_GC
Cuts at: 0 206 473 754 896 1838 1886 3168
Size: 206 267 281 142 942 48 1282
Fragments arranged by size:
1282 942 281 267 206 142 48
BglI GCCn_nnn'nGGC
Cuts at: 0 163 2222 2353 2810 3168
Size: 163 2059 131 457 358
Fragments arranged by size:
2059 457 358 163 131
BglII A'GATC_T
Cuts at: 0 3005 3168
Size: 3005 163
BlpI GC'TnA_GC
Cuts at: 0 2827 3168
Size: 2827 341
Bme1580I G_kGCm'C
Cuts at: 0 237 886 1618 2008 2227 2685 2983 3132
Size: 237 649 732 390 219 458 298 149
Cuts at: 3132 3168
Size: 36
Fragments arranged by size:
732 649 458 390 298 237 219 149
36
BmgBI CAC'GTC
Cuts at: 0 515 3168
Size: 515 2653
BmrI ACTGGGnnnn_n'
Cuts at: 0 2631 3168
Size: 2631 537
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 846 1194 1746 3168
Size: 846 348 552 1422
Fragments arranged by size:
1422 846 552 348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 1387 1394 1699 1984 3168
Size: 181 1206 7 305 285 1184
Fragments arranged by size:
1206 1184 305 285 181 7
Bpu10I CC'TnA_GC
Cuts at: 0 206 473 493 754 896 1696 1838 1886
Size: 206 267 20 261 142 800 142 48
Cuts at: 1886 3168
Size: 1282
Fragments arranged by size:
1282 800 267 261 206 142 142 48
20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1085 1430 2914 3168
Size: 1085 345 1484 254
Fragments arranged by size:
1484 1085 345 254
BsaI GGTCTCn'nnnn_
Cuts at: 0 917 1432 1606 2179 3168
Size: 917 515 174 573 989
Fragments arranged by size:
989 917 573 515 174
BsaAI yAC'GTr
Cuts at: 0 99 1116 3168
Size: 99 1017 2052
Fragments arranged by size:
2052 1017 99
BsaHI Gr'CG_yC
Cuts at: 0 1138 1948 2813 3168
Size: 1138 810 865 355
Fragments arranged by size:
1138 865 810 355
BsaWI w'CCGG_w
Cuts at: 0 908 1565 3168
Size: 908 657 1603
Fragments arranged by size:
1603 908 657
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 469 499 847 877 1885 1915 3168
Size: 469 30 348 30 1008 30 1253
Fragments arranged by size:
1253 1008 469 348 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 490 738 868 1720 1812 2365 2772 3168
Size: 490 248 130 852 92 553 407 396
Fragments arranged by size:
852 553 490 407 396 248 130 92
BseYI C'CCAG_C
Cuts at: 0 254 779 1141 1611 2684 2954 2984 3168
Size: 254 525 362 470 1073 270 30 184
Fragments arranged by size:
1073 525 470 362 270 254 184 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 171 653 1033 1138 1933 3168
Size: 169 2 482 380 105 795 1235
Fragments arranged by size:
1235 795 482 380 169 105 2
BsiHKAI G_wGCw'C
Cuts at: 0 320 1177 1658 1670 1712 2828 2983 3168
Size: 320 857 481 12 42 1116 155 185
Fragments arranged by size:
1116 857 481 320 185 155 42 12
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2723 2754 3168
Size: 2723 31 414
Fragments arranged by size:
2723 414 31
Bsp1286I G_dGCh'C
Cuts at: 0 237 320 860 886 1073 1177 1618 1658
Size: 237 83 540 26 187 104 441 40
Cuts at: 1658 1670 1712 1991 2008 2227 2685 2828 2983
Size: 12 42 279 17 219 458 143 155
Cuts at: 2983 3132 3168
Size: 149 36
Fragments arranged by size:
540 458 441 279 237 219 187 155
149 143 104 83 42 40 36 26
17 12
BspHI T'CATG_A
Cuts at: 0 1260 1604 3168
Size: 1260 344 1564
Fragments arranged by size:
1564 1260 344
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1055 1071 1985 2708 3168
Size: 1055 16 914 723 460
Fragments arranged by size:
1055 914 723 460 16
BsrBI CCG'CTC
Cuts at: 0 1626 2739 3168
Size: 1626 1113 429
Fragments arranged by size:
1626 1113 429
BsrDI GCAATG_nn'
Cuts at: 0 846 1643 3168
Size: 846 797 1525
Fragments arranged by size:
1525 846 797
BsrFI r'CCGG_y
Cuts at: 0 908 1343 3134 3168
Size: 908 435 1791 34
Fragments arranged by size:
1791 908 435 34
BsrGI T'GTAC_A
Cuts at: 0 2075 3168
Size: 2075 1093
BssHII G'CGCG_C
Cuts at: 0 1815 3168
Size: 1815 1353
BstBI TT'CG_AA
Cuts at: 0 527 3168
Size: 527 2641
BstEII G'GTnAC_C
Cuts at: 0 2358 3168
Size: 2358 810
BstXI CCAn_nnnn'nTGG
Cuts at: 0 826 1149 1224 1237 1771 3168
Size: 826 323 75 13 534 1397
Fragments arranged by size:
1397 826 534 323 75 13
BstYI r'GATC_y
Cuts at: 0 70 992 1322 2307 2675 3005 3168
Size: 70 922 330 985 368 330 163
Fragments arranged by size:
985 922 368 330 330 163 70
Bsu36I CC'TnA_GG
Cuts at: 0 1179 1931 3168
Size: 1179 752 1237
Fragments arranged by size:
1237 1179 752
BtgI C'CryG_G
Cuts at: 0 177 1023 1619 1991 2175 2456 3168
Size: 177 846 596 372 184 281 712
Fragments arranged by size:
846 712 596 372 281 184 177
BtsI GCAGTG_nn'
Cuts at: 0 2967 3168
Size: 2967 201
DraIII CAC_nnn'GTG
Cuts at: 0 149 2181 3168
Size: 149 2032 987
Fragments arranged by size:
2032 987 149
DrdI GACnn_nn'nnGTC
Cuts at: 0 115 697 1413 3052 3168
Size: 115 582 716 1639 116
Fragments arranged by size:
1639 716 582 116 115
EaeI y'GGCC_r
Cuts at: 0 767 2249 2603 3168
Size: 767 1482 354 565
Fragments arranged by size:
1482 767 565 354
EarI CTCTTCn'nnn_
Cuts at: 0 1663 3168
Size: 1663 1505
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1675 2016 3168
Size: 1675 341 1152
Fragments arranged by size:
1675 1152 341
EcoICRI GAG'CTC
Cuts at: 0 1656 1668 2826 3168
Size: 1656 12 1158 342
Fragments arranged by size:
1656 1158 342 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 424 662 707 1297 1361 1387 1394
Size: 181 243 238 45 590 64 26 7
Cuts at: 1394 1699 1984 2284 2680 2794 2839 3168
Size: 305 285 300 396 114 45 329
Fragments arranged by size:
590 396 329 305 300 285 243 238
181 114 64 45 45 26 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1246 1502 1873 3168
Size: 1246 256 371 1295
Fragments arranged by size:
1295 1246 371 256
EcoO109I rG'GnC_Cy
Cuts at: 0 507 1866 2087 2223 2625 2655 2934 3168
Size: 507 1359 221 136 402 30 279 234
Fragments arranged by size:
1359 507 402 279 234 221 136 30
EcoRI G'AATT_C
Cuts at: 0 934 3168
Size: 934 2234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1674 3096 3168
Size: 1674 1422 72
Fragments arranged by size:
1674 1422 72
FspI TGC'GCA
Cuts at: 0 2493 3168
Size: 2493 675
HaeII r_GCGC'y
Cuts at: 0 158 1141 1779 1951 2640 2816 3168
Size: 158 983 638 172 689 176 352
Fragments arranged by size:
983 689 638 352 176 172 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 604 636 847 879 1966 1998 2008 2040
Size: 604 32 211 32 1087 32 10 32
Cuts at: 2040 2522 2554 2575 2607 3168
Size: 482 32 21 32 561
Fragments arranged by size:
1087 604 561 482 211 32 32 32
32 32 32 21 10
HincII GTy'rAC
Cuts at: 0 333 3168
Size: 333 2835
KasI G'GCGC_C
Cuts at: 0 1137 1947 2812 3168
Size: 1137 810 865 356
Fragments arranged by size:
1137 865 810 356
MluI A'CGCG_T
Cuts at: 0 2714 3168
Size: 2714 454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 911 1610 2919 3168
Size: 293 618 699 1309 249
Fragments arranged by size:
1309 699 618 293 249
MslI CAynn'nnrTG
Cuts at: 0 340 1715 1717 1769 3168
Size: 340 1375 2 52 1399
Fragments arranged by size:
1399 1375 340 52 2
MspA1I CmG'CkG
Cuts at: 0 350 645 783 1677 1993 2772 3125 3168
Size: 350 295 138 894 316 779 353 43
Fragments arranged by size:
894 779 353 350 316 295 138 43
NaeI GCC'GGC
Cuts at: 0 3136 3168
Size: 3136 32
NarI GG'CG_CC
Cuts at: 0 1138 1948 2813 3168
Size: 1138 810 865 355
Fragments arranged by size:
1138 865 810 355
NcoI C'CATG_G
Cuts at: 0 177 1023 2456 3168
Size: 177 846 1433 712
Fragments arranged by size:
1433 846 712 177
NgoMIV G'CCGG_C
Cuts at: 0 3134 3168
Size: 3134 34
NspI r_CATG'y
Cuts at: 0 2734 2962 3168
Size: 2734 228 206
Fragments arranged by size:
2734 228 206
PasI CC'CwG_GG
Cuts at: 0 1470 1536 1953 2364 3168
Size: 1470 66 417 411 804
Fragments arranged by size:
1470 804 417 411 66
PflMI CCAn_nnn'nTGG
Cuts at: 0 2181 3102 3168
Size: 2181 921 66
Fragments arranged by size:
2181 921 66
PfoI T'CCnGG_A
Cuts at: 0 464 2192 3168
Size: 464 1728 976
Fragments arranged by size:
1728 976 464
PmlI CAC'GTG
Cuts at: 0 1116 3168
Size: 1116 2052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 469 501 3168
Size: 469 32 2667
Fragments arranged by size:
2667 469 32
PpuMI rG'GwC_Cy
Cuts at: 0 2087 2625 3168
Size: 2087 538 543
Fragments arranged by size:
2087 543 538
PshAI GACnn'nnGTC
Cuts at: 0 328 3168
Size: 328 2840
PspOMI G'GGCC_C
Cuts at: 0 2223 2681 3168
Size: 2223 458 487
Fragments arranged by size:
2223 487 458
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1900 1932 3168
Size: 1900 32 1236
Fragments arranged by size:
1900 1236 32
PstI C_TGCA'G
Cuts at: 0 419 506 793 1068 1247 1874 2273 2438
Size: 419 87 287 275 179 627 399 165
Cuts at: 2438 2650 2705 3168
Size: 212 55 463
Fragments arranged by size:
627 463 419 399 287 275 212 179
165 87 55
PvuII CAG'CTG
Cuts at: 0 783 2772 3168
Size: 783 1989 396
Fragments arranged by size:
1989 783 396
SacI G_AGCT'C
Cuts at: 0 1658 1670 2828 3168
Size: 1658 12 1158 340
Fragments arranged by size:
1658 1158 340 12
SacII CC_GC'GG
Cuts at: 0 1994 3168
Size: 1994 1174
SapI GCTCTTCn'nnn_
Cuts at: 0 1663 3168
Size: 1663 1505
SbfI CC_TGCA'GG
Cuts at: 0 506 3168
Size: 506 2662
ScaI AGT'ACT
Cuts at: 0 442 1200 3168
Size: 442 758 1968
Fragments arranged by size:
1968 758 442
SexAI A'CCwGG_T
Cuts at: 0 2441 3168
Size: 2441 727
SfcI C'TryA_G
Cuts at: 0 415 502 640 789 1064 1166 1243 1870
Size: 415 87 138 149 275 102 77 627
Cuts at: 1870 2269 2434 2646 2701 3168
Size: 399 165 212 55 467
Fragments arranged by size:
627 467 415 399 275 212 165 149
138 102 87 77 55
SfoI GGC'GCC
Cuts at: 0 1139 1949 2814 3168
Size: 1139 810 865 354
Fragments arranged by size:
1139 865 810 354
SgrAI Cr'CCGG_yG
Cuts at: 0 3134 3168
Size: 3134 34
SmaI CCC'GGG
Cuts at: 0 1354 2416 3168
Size: 1354 1062 752
Fragments arranged by size:
1354 1062 752
SmlI C'TyrA_G
Cuts at: 0 1100 1445 2929 3168
Size: 1100 345 1484 239
Fragments arranged by size:
1484 1100 345 239
SpeI A'CTAG_T
Cuts at: 0 121 3168
Size: 121 3047
StuI AGG'CCT
Cuts at: 0 82 1009 1422 2468 3168
Size: 82 927 413 1046 700
Fragments arranged by size:
1046 927 700 413 82
StyI C'CwwG_G
Cuts at: 0 177 1023 1230 2117 2217 2252 2456 2478
Size: 177 846 207 887 100 35 204 22
Cuts at: 2478 3168
Size: 690
Fragments arranged by size:
887 846 690 207 204 177 100 35
22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 304 1311 1400 1893 2998 3168
Size: 304 1007 89 493 1105 170
Fragments arranged by size:
1105 1007 493 304 170 89
TatI w'GTAC_w
Cuts at: 0 440 1198 2069 2075 3168
Size: 440 758 871 6 1093
Fragments arranged by size:
1093 871 758 440 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 216 248 1885 1917 3168
Size: 216 32 1637 32 1251
Fragments arranged by size:
1637 1251 216 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1090 3168
Size: 1090 2078
XmaI C'CCGG_G
Cuts at: 0 1352 2414 3168
Size: 1352 1062 754
Fragments arranged by size:
1352 1062 754
XmnI GAAnn'nnTTC
Cuts at: 0 2134 2425 3160 3168
Size: 2134 291 735 8
Fragments arranged by size:
2134 735 291 8
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI MluI
MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI
PsrI PstI PvuII SacI SacII SapI SbfI ScaI
SexAI SfcI SfoI SgrAI SmaI SmlI SpeI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AscI AseI AsiSI
AvrII BaeI BcgI BciVI BclI BfrBI BmtI BsaBI
BsiEI BsiWI BsmI BspEI BssSI BstAPI BstZ17I BtgZI
ClaI CspCI DraI EagI EcoRV FseI FspAI HindIII
HpaI KpnI MfeI MscI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PsiI PspXI PvuI RsrII
SalI SanDI SfiI SgfI SnaBI SphI SrfI SspI
SwaI Tth111I XbaI XhoI ZraI