Restriction map for
pF1KA0358
(Linear) (Six-base) MAPSORT of: KIAA0358.seq Check: 6562 from: 1 to: 4743
KIAA0358.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1081 3646 4668 4743
Size: 1081 2565 1022 75
Fragments arranged by size:
2565 1081 1022 75
AatII G_ACGT'C
Cuts at: 0 2262 3505 4743
Size: 2262 1243 1238
Fragments arranged by size:
2262 1243 1238
AccI GT'mk_AC
Cuts at: 0 580 800 4743
Size: 580 220 3943
Fragments arranged by size:
3943 580 220
Acc65I G'GTAC_C
Cuts at: 0 4052 4743
Size: 4052 691
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2565 2708 2854 3440 3605 3845 4672 4743
Size: 2565 143 146 586 165 240 827 71
Fragments arranged by size:
2565 827 586 240 165 146 143 71
AfeI AGC'GCT
Cuts at: 0 4429 4743
Size: 4429 314
AflIII A'CryG_T
Cuts at: 0 1528 2394 4743
Size: 1528 866 2349
Fragments arranged by size:
2349 1528 866
AhdI GACnn_n'nnGTC
Cuts at: 0 1395 4463 4743
Size: 1395 3068 280
Fragments arranged by size:
3068 1395 280
AleI CACnn'nnGTG
Cuts at: 0 874 3598 4743
Size: 874 2724 1145
Fragments arranged by size:
2724 1145 874
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3092 4743
Size: 3092 1651
AlwNI CAG_nnn'CTG
Cuts at: 0 1098 2832 4650 4725 4743
Size: 1098 1734 1818 75 18
Fragments arranged by size:
1818 1734 1098 75 18
ApaLI G'TGCA_C
Cuts at: 0 2845 4743
Size: 2845 1898
ApoI r'AATT_y
Cuts at: 0 1339 1414 1694 3342 4705 4743
Size: 1339 75 280 1648 1363 38
Fragments arranged by size:
1648 1363 1339 280 75 38
AseI AT'TA_AT
Cuts at: 0 1949 4743
Size: 1949 2794
AvaI C'yCGr_G
Cuts at: 0 66 172 470 644 977 2477 2618 2698
Size: 66 106 298 174 333 1500 141 80
Cuts at: 2698 3278 3701 4743
Size: 580 423 1042
Fragments arranged by size:
1500 1042 580 423 333 298 174 141
106 80 66
AvrII C'CTAG_G
Cuts at: 0 3864 4743
Size: 3864 879
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1515 1548 2556 2589 4743
Size: 1515 33 1008 33 2154
Fragments arranged by size:
2154 1515 1008 33 33
BamHI G'GATC_C
Cuts at: 0 1661 3210 3880 4743
Size: 1661 1549 670 863
Fragments arranged by size:
1661 1549 863 670
BanI G'GyrC_C
Cuts at: 0 62 2614 3282 4052 4190 4743
Size: 62 2552 668 770 138 553
Fragments arranged by size:
2552 770 668 553 138 62
BanII G_rGCy'C
Cuts at: 0 406 827 2324 2415 4743
Size: 406 421 1497 91 2328
Fragments arranged by size:
2328 1497 421 406 91
BbeI G_GCGC'C
Cuts at: 0 4194 4743
Size: 4194 549
BbsI GAAGACnn'nnnn_
Cuts at: 0 225 4660 4743
Size: 225 4435 83
Fragments arranged by size:
4435 225 83
BbvCI CC'TCA_GC
Cuts at: 0 1805 3058 4743
Size: 1805 1253 1685
Fragments arranged by size:
1805 1685 1253
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 712 746 4743
Size: 712 34 3997
Fragments arranged by size:
3997 712 34
BclI T'GATC_A
Cuts at: 0 3522 4251 4743
Size: 3522 729 492
Fragments arranged by size:
3522 729 492
BglI GCCn_nnn'nGGC
Cuts at: 0 623 4743
Size: 623 4120
BlpI GC'TnA_GC
Cuts at: 0 538 4743
Size: 538 4205
Bme1580I G_kGCm'C
Cuts at: 0 717 2619 2849 3285 3709 4743
Size: 717 1902 230 436 424 1034
Fragments arranged by size:
1902 1034 717 436 424 230
BmgBI CAC'GTC
Cuts at: 0 524 1594 4743
Size: 524 1070 3149
Fragments arranged by size:
3149 1070 524
BmrI ACTGGGnnnn_n'
Cuts at: 0 643 766 2002 2636 3193 3280 4743
Size: 643 123 1236 634 557 87 1463
Fragments arranged by size:
1463 1236 643 634 557 123 87
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 432 1851 2490 3753 3865 4743
Size: 432 1419 639 1263 112 878
Fragments arranged by size:
1419 1263 878 639 432 112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 811 913 1354 2082 2929 3635 4743
Size: 278 533 102 441 728 847 706 1108
Fragments arranged by size:
1108 847 728 706 533 441 278 102
Bpu10I CC'TnA_GC
Cuts at: 0 184 1805 2938 3058 4743
Size: 184 1621 1133 120 1685
Fragments arranged by size:
1685 1621 1133 184 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 2460 3028 3555 3663 4743
Size: 571 1889 568 527 108 1080
Fragments arranged by size:
1889 1080 571 568 527 108
BsaI GGTCTCn'nnnn_
Cuts at: 0 3613 3724 3910 4504 4743
Size: 3613 111 186 594 239
Fragments arranged by size:
3613 594 239 186 111
BsaBI GATnn'nnATC
Cuts at: 0 4100 4743
Size: 4100 643
BsaHI Gr'CG_yC
Cuts at: 0 920 2201 2259 3502 4191 4743
Size: 920 1281 58 1243 689 552
Fragments arranged by size:
1281 1243 920 689 552 58
BsaWI w'CCGG_w
Cuts at: 0 4076 4743
Size: 4076 667
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1825 1855 3451 3481 4120 4150 4743
Size: 1825 30 1596 30 639 30 593
Fragments arranged by size:
1825 1596 639 593 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 458 715 1896 3031 3472 4743
Size: 458 257 1181 1135 441 1271
Fragments arranged by size:
1271 1181 1135 458 441 257
BseYI C'CCAG_C
Cuts at: 0 1316 1796 2128 2230 3223 4514 4743
Size: 1316 480 332 102 993 1291 229
Fragments arranged by size:
1316 1291 993 480 332 229 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 2980 4108 4663 4743
Size: 874 2106 1128 555 80
Fragments arranged by size:
2106 1128 874 555 80
BsiHKAI G_wGCw'C
Cuts at: 0 406 539 827 959 2849 4743
Size: 406 133 288 132 1890 1894
Fragments arranged by size:
1894 1890 406 288 133 132
BsmI GAATG_Cn'
Cuts at: 0 944 1012 4743
Size: 944 68 3731
Fragments arranged by size:
3731 944 68
BsmBI CGTCTCn'nnnn_
Cuts at: 0 786 2208 2508 4743
Size: 786 1422 300 2235
Fragments arranged by size:
2235 1422 786 300
Bsp1286I G_dGCh'C
Cuts at: 0 406 539 717 827 959 2324 2415 2619
Size: 406 133 178 110 132 1365 91 204
Cuts at: 2619 2849 3285 3709 4743
Size: 230 436 424 1034
Fragments arranged by size:
1365 1034 436 424 406 230 204 178
133 132 110 91
BspHI T'CATG_A
Cuts at: 0 1344 4743
Size: 1344 3399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 949 1081 1400 2169 3646 4668 4743
Size: 949 132 319 769 1477 1022 75
Fragments arranged by size:
1477 1022 949 769 319 132 75
BsrBI CCG'CTC
Cuts at: 0 296 503 1830 4743
Size: 296 207 1327 2913
Fragments arranged by size:
2913 1327 296 207
BsrDI GCAATG_nn'
Cuts at: 0 1028 3657 4743
Size: 1028 2629 1086
Fragments arranged by size:
2629 1086 1028
BsrFI r'CCGG_y
Cuts at: 0 2291 2692 2900 4304 4743
Size: 2291 401 208 1404 439
Fragments arranged by size:
2291 1404 439 401 208
BsrGI T'GTAC_A
Cuts at: 0 3020 3049 4223 4743
Size: 3020 29 1174 520
Fragments arranged by size:
3020 1174 520 29
BssHII G'CGCG_C
Cuts at: 0 4581 4743
Size: 4581 162
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2853 3289 4743
Size: 2853 436 1454
Fragments arranged by size:
2853 1454 436
BstEII G'GTnAC_C
Cuts at: 0 4680 4743
Size: 4680 63
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1077 2010 2872 4743
Size: 1077 933 862 1871
Fragments arranged by size:
1871 1077 933 862
BstYI r'GATC_y
Cuts at: 0 674 749 1191 1358 1661 3210 3880 4743
Size: 674 75 442 167 303 1549 670 863
Fragments arranged by size:
1549 863 674 670 442 303 167 75
BstZ17I GTA'TAC
Cuts at: 0 581 4743
Size: 581 4162
Bsu36I CC'TnA_GG
Cuts at: 0 2342 3308 4743
Size: 2342 966 1435
Fragments arranged by size:
2342 1435 966
BtgI C'CryG_G
Cuts at: 0 348 1475 2142 3596 4743
Size: 348 1127 667 1454 1147
Fragments arranged by size:
1454 1147 1127 667 348
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4397 4743
Size: 4397 346
BtsI GCAGTG_nn'
Cuts at: 0 78 886 1872 2089 2599 3687 4743
Size: 78 808 986 217 510 1088 1056
Fragments arranged by size:
1088 1056 986 808 510 217 78
ClaI AT'CG_AT
Cuts at: 0 756 4743
Size: 756 3987
DraI TTT'AAA
Cuts at: 0 1283 3332 4743
Size: 1283 2049 1411
Fragments arranged by size:
2049 1411 1283
DraIII CAC_nnn'GTG
Cuts at: 0 668 963 2853 3289 4743
Size: 668 295 1890 436 1454
Fragments arranged by size:
1890 1454 668 436 295
DrdI GACnn_nn'nnGTC
Cuts at: 0 259 808 4743
Size: 259 549 3935
Fragments arranged by size:
3935 549 259
EaeI y'GGCC_r
Cuts at: 0 1295 1625 2862 3101 4112 4743
Size: 1295 330 1237 239 1011 631
Fragments arranged by size:
1295 1237 1011 631 330 239
EarI CTCTTCn'nnn_
Cuts at: 0 380 854 1902 2042 3163 4743
Size: 380 474 1048 140 1121 1580
Fragments arranged by size:
1580 1121 1048 474 380 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 687 1485 4743
Size: 687 798 3258
Fragments arranged by size:
3258 798 687
EcoICRI GAG'CTC
Cuts at: 0 404 825 4743
Size: 404 421 3918
Fragments arranged by size:
3918 421 404
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 811 913 1354 2082 2565 2708 2854
Size: 278 533 102 441 728 483 143 146
Cuts at: 2854 2929 3440 3605 3635 3845 4672 4743
Size: 75 511 165 30 210 827 71
Fragments arranged by size:
827 728 533 511 483 441 278 210
165 146 143 102 75 71 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2836 4675 4743
Size: 2836 1839 68
Fragments arranged by size:
2836 1839 68
EcoO109I rG'GnC_Cy
Cuts at: 0 344 810 1809 3234 3274 3304 4030 4647
Size: 344 466 999 1425 40 30 726 617
Cuts at: 4647 4743
Size: 96
Fragments arranged by size:
1425 999 726 617 466 344 96 40
30
EcoRI G'AATT_C
Cuts at: 0 1414 1694 4743
Size: 1414 280 3049
Fragments arranged by size:
3049 1414 280
EcoRV GAT'ATC
Cuts at: 0 4362 4743
Size: 4362 381
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1308 1916 4743
Size: 1308 608 2827
Fragments arranged by size:
2827 1308 608
HaeII r_GCGC'y
Cuts at: 0 594 618 4086 4194 4431 4743
Size: 594 24 3468 108 237 312
Fragments arranged by size:
3468 594 312 237 108 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 260 292 691 723 788 820
Size: 208 32 20 32 399 32 65 32
Cuts at: 820 831 863 1381 1413 1825 1857 1879 1911
Size: 11 32 518 32 412 32 22 32
Cuts at: 1911 2452 2484 2725 2757 2918 2950 3451 3483
Size: 541 32 241 32 161 32 501 32
Cuts at: 3483 3507 3514 3539 3546 3865 3897 4265 4297
Size: 24 7 25 7 319 32 368 32
Cuts at: 4297 4449 4481 4743
Size: 152 32 262
Fragments arranged by size:
541 518 501 412 399 368 319 262
241 208 161 152 65 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 25 24 22 20
11 7 7
HincII GTy'rAC
Cuts at: 0 285 4743
Size: 285 4458
HindIII A'AGCT_T
Cuts at: 0 3398 4743
Size: 3398 1345
HpaI GTT'AAC
Cuts at: 0 285 4743
Size: 285 4458
KasI G'GCGC_C
Cuts at: 0 4190 4743
Size: 4190 553
KpnI G_GTAC'C
Cuts at: 0 4056 4743
Size: 4056 687
MluI A'CGCG_T
Cuts at: 0 1528 4743
Size: 1528 3215
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1132 2531 2992 3202 3980 4743
Size: 1132 1399 461 210 778 763
Fragments arranged by size:
1399 1132 778 763 461 210
MscI TGG'CCA
Cuts at: 0 1297 1627 3103 4114 4743
Size: 1297 330 1476 1011 629
Fragments arranged by size:
1476 1297 1011 629 330
MslI CAynn'nnrTG
Cuts at: 0 874 1003 1075 2399 3541 3598 4375 4453
Size: 874 129 72 1324 1142 57 777 78
Cuts at: 4453 4743
Size: 290
Fragments arranged by size:
1324 1142 874 777 290 129 78 72
57
MspA1I CmG'CkG
Cuts at: 0 198 1062 1095 1758 1800 2922 3553 4257
Size: 198 864 33 663 42 1122 631 704
Cuts at: 4257 4743
Size: 486
Fragments arranged by size:
1122 864 704 663 631 486 198 42
33
NaeI GCC'GGC
Cuts at: 0 2293 2694 2902 4306 4743
Size: 2293 401 208 1404 437
Fragments arranged by size:
2293 1404 437 401 208
NarI GG'CG_CC
Cuts at: 0 4191 4743
Size: 4191 552
NcoI C'CATG_G
Cuts at: 0 1475 4743
Size: 1475 3268
NgoMIV G'CCGG_C
Cuts at: 0 2291 2692 2900 4304 4743
Size: 2291 401 208 1404 439
Fragments arranged by size:
2291 1404 439 401 208
NspI r_CATG'y
Cuts at: 0 1987 2398 4146 4339 4743
Size: 1987 411 1748 193 404
Fragments arranged by size:
1987 1748 411 404 193
PasI CC'CwG_GG
Cuts at: 0 2793 4743
Size: 2793 1950
PciI A'CATG_T
Cuts at: 0 2394 4743
Size: 2394 2349
PfoI T'CCnGG_A
Cuts at: 0 2993 4743
Size: 2993 1750
PpuMI rG'GwC_Cy
Cuts at: 0 344 810 3234 3274 3304 4030 4647 4743
Size: 344 466 2424 40 30 726 617 96
Fragments arranged by size:
2424 726 617 466 344 96 40 30
PshAI GACnn'nnGTC
Cuts at: 0 923 2258 4028 4743
Size: 923 1335 1770 715
Fragments arranged by size:
1770 1335 923 715
PspXI vC'TCGA_Gb
Cuts at: 0 644 4743
Size: 644 4099
PstI C_TGCA'G
Cuts at: 0 428 1397 1511 1763 2030 4679 4743
Size: 428 969 114 252 267 2649 64
Fragments arranged by size:
2649 969 428 267 252 114 64
PvuII CAG'CTG
Cuts at: 0 1095 1758 1800 2922 3553 4257 4743
Size: 1095 663 42 1122 631 704 486
Fragments arranged by size:
1122 1095 704 663 631 486 42
SacI G_AGCT'C
Cuts at: 0 406 827 4743
Size: 406 421 3916
Fragments arranged by size:
3916 421 406
SanDI GG'GwC_CC
Cuts at: 0 344 3234 4030 4743
Size: 344 2890 796 713
Fragments arranged by size:
2890 796 713 344
SapI GCTCTTCn'nnn_
Cuts at: 0 854 1902 2042 4743
Size: 854 1048 140 2701
Fragments arranged by size:
2701 1048 854 140
ScaI AGT'ACT
Cuts at: 0 516 1648 4743
Size: 516 1132 3095
Fragments arranged by size:
3095 1132 516
SexAI A'CCwGG_T
Cuts at: 0 3666 4743
Size: 3666 1077
SfcI C'TryA_G
Cuts at: 0 424 606 970 1393 1507 1759 2026 3144
Size: 424 182 364 423 114 252 267 1118
Cuts at: 3144 4675 4743
Size: 1531 68
Fragments arranged by size:
1531 1118 424 423 364 267 252 182
114 68
SfoI GGC'GCC
Cuts at: 0 4192 4743
Size: 4192 551
SgrAI Cr'CCGG_yG
Cuts at: 0 2291 4743
Size: 2291 2452
SmaI CCC'GGG
Cuts at: 0 2700 4743
Size: 2700 2043
SmlI C'TyrA_G
Cuts at: 0 586 644 2439 3043 3570 3642 4743
Size: 586 58 1795 604 527 72 1101
Fragments arranged by size:
1795 1101 604 586 527 72 58
StuI AGG'CCT
Cuts at: 0 745 1291 1380 3417 4743
Size: 745 546 89 2037 1326
Fragments arranged by size:
2037 1326 745 546 89
StyI C'CwwG_G
Cuts at: 0 116 488 899 1292 1475 1911 3444 3624
Size: 116 372 411 393 183 436 1533 180
Cuts at: 3624 3864 4062 4743
Size: 240 198 681
Fragments arranged by size:
1533 681 436 411 393 372 240 198
183 180 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1330 3082 4615 4743
Size: 1330 1752 1533 128
Fragments arranged by size:
1752 1533 1330 128
TatI w'GTAC_w
Cuts at: 0 514 1646 3020 3049 4223 4553 4743
Size: 514 1132 1374 29 1174 330 190
Fragments arranged by size:
1374 1174 1132 514 330 190 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 120 152 652 684 4743
Size: 120 32 500 32 4059
Fragments arranged by size:
4059 500 120 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1921 3532 4743
Size: 1921 1611 1211
Fragments arranged by size:
1921 1611 1211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1005 1329 1473 2398 4743
Size: 1005 324 144 925 2345
Fragments arranged by size:
2345 1005 925 324 144
XhoI C'TCGA_G
Cuts at: 0 644 4743
Size: 644 4099
XmaI C'CCGG_G
Cuts at: 0 2698 4743
Size: 2698 2045
XmnI GAAnn'nnTTC
Cuts at: 0 16 1380 4743
Size: 16 1364 3363
Fragments arranged by size:
3363 1364 16
ZraI GAC'GTC
Cuts at: 0 2260 3503 4743
Size: 2260 1243 1240
Fragments arranged by size:
2260 1243 1240
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PpuMI PshAI PspXI PstI PvuII SacI SanDI SapI
ScaI SexAI SfcI SfoI SgrAI SmaI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI AloI ApaI AscI AsiSI BciVI
BfrBI BglII BmtI BsaAI BsiEI BsiWI BspEI BssSI
BstBI CspCI EagI FseI FspI FspAI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PmeI PmlI
PpiI PsiI PspOMI PsrI PvuI RsrII SacII SalI
SbfI SfiI SgfI SnaBI SpeI SphI SrfI SspI
SwaI XbaI