Restriction map for
pF1KSDA0360
(Linear) (Six-base) MAPSORT of: KIAA0360.seq Check: 4925 from: 1 to: 3021
KIAA0360.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 606 3021
Size: 606 2415
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 708 776 1063 2198 3021
Size: 347 361 68 287 1135 823
Fragments arranged by size:
1135 823 361 347 287 68
AflIII A'CryG_T
Cuts at: 0 1284 3021
Size: 1284 1737
AleI CACnn'nnGTG
Cuts at: 0 1187 2659 3021
Size: 1187 1472 362
Fragments arranged by size:
1472 1187 362
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 634 1363 3021
Size: 634 729 1658
Fragments arranged by size:
1658 729 634
AlwNI CAG_nnn'CTG
Cuts at: 0 304 400 621 691 1782 3021
Size: 304 96 221 70 1091 1239
Fragments arranged by size:
1239 1091 304 221 96 70
ApaI G_GGCC'C
Cuts at: 0 340 1935 3021
Size: 340 1595 1086
Fragments arranged by size:
1595 1086 340
ApaLI G'TGCA_C
Cuts at: 0 2024 3021
Size: 2024 997
ApoI r'AATT_y
Cuts at: 0 142 2070 3021
Size: 142 1928 951
Fragments arranged by size:
1928 951 142
AvaI C'yCGr_G
Cuts at: 0 1007 1928 2060 3021
Size: 1007 921 132 961
Fragments arranged by size:
1007 961 921 132
BamHI G'GATC_C
Cuts at: 0 324 3021
Size: 324 2697
BanI G'GyrC_C
Cuts at: 0 652 3021
Size: 652 2369
BanII G_rGCy'C
Cuts at: 0 340 1040 1600 1742 1842 1935 2984 3021
Size: 340 700 560 142 100 93 1049 37
Fragments arranged by size:
1049 700 560 340 142 100 93 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 353 2495 2797 3021
Size: 353 2142 302 224
Fragments arranged by size:
2142 353 302 224
BbvCI CC'TCA_GC
Cuts at: 0 1824 1872 2546 3021
Size: 1824 48 674 475
Fragments arranged by size:
1824 674 475 48
BlpI GC'TnA_GC
Cuts at: 0 1069 1599 1918 2680 3021
Size: 1069 530 319 762 341
Fragments arranged by size:
1069 762 530 341 319
Bme1580I G_kGCm'C
Cuts at: 0 320 340 657 1161 1683 1935 2028 2067
Size: 320 20 317 504 522 252 93 39
Cuts at: 2067 3021
Size: 954
Fragments arranged by size:
954 522 504 320 317 252 93 39
20
BmrI ACTGGGnnnn_n'
Cuts at: 0 850 1300 3021
Size: 850 450 1721
Fragments arranged by size:
1721 850 450
BmtI G_CTAG'C
Cuts at: 0 83 3021
Size: 83 2938
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1487 2223 2631 3021
Size: 1487 736 408 390
Fragments arranged by size:
1487 736 408 390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 887 1385 1490 2797 3021
Size: 443 444 498 105 1307 224
Fragments arranged by size:
1307 498 444 443 224 105
Bpu10I CC'TnA_GC
Cuts at: 0 1824 1872 2195 2546 3021
Size: 1824 48 323 351 475
Fragments arranged by size:
1824 475 351 323 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1036 2601 2848 2869 3021
Size: 1036 1565 247 21 152
Fragments arranged by size:
1565 1036 247 152 21
BsaWI w'CCGG_w
Cuts at: 0 2129 2271 2604 3021
Size: 2129 142 333 417
Fragments arranged by size:
2129 417 333 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 162 192 3021
Size: 162 30 2829
Fragments arranged by size:
2829 162 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 222 360 365 370 473 476 762
Size: 100 122 138 5 5 103 3 286
Cuts at: 762 765 768 771 1399 1402 2201 2293 2305
Size: 3 3 3 628 3 799 92 12
Cuts at: 2305 2308 2317 2440 2443 2689 2794 3021
Size: 3 9 123 3 246 105 227
Fragments arranged by size:
799 628 286 246 227 138 123 122
105 103 100 92 12 9 5 5
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 55 400 438 909 1676 2246 2258 2461
Size: 55 345 38 471 767 570 12 203
Cuts at: 2461 3021
Size: 560
Fragments arranged by size:
767 570 560 471 345 203 55 38
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 1309 1508 1844 2153 3021
Size: 458 851 199 336 309 868
Fragments arranged by size:
868 851 458 336 309 199
BsiHKAI G_wGCw'C
Cuts at: 0 1319 1538 2028 3021
Size: 1319 219 490 993
Fragments arranged by size:
1319 993 490 219
BsmI GAATG_Cn'
Cuts at: 0 958 3021
Size: 958 2063
Bsp1286I G_dGCh'C
Cuts at: 0 320 340 657 1040 1161 1319 1538 1600
Size: 320 20 317 383 121 158 219 62
Cuts at: 1600 1683 1742 1842 1935 2028 2067 2984 3021
Size: 83 59 100 93 93 39 917 37
Fragments arranged by size:
917 383 320 317 219 158 121 100
93 93 83 62 59 39 37 20
BspEI T'CCGG_A
Cuts at: 0 2129 3021
Size: 2129 892
BspHI T'CATG_A
Cuts at: 0 1538 3021
Size: 1538 1483
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 606 3021
Size: 606 2415
BsrBI CCG'CTC
Cuts at: 0 2814 2853 3021
Size: 2814 39 168
Fragments arranged by size:
2814 168 39
BsrDI GCAATG_nn'
Cuts at: 0 1215 2702 3021
Size: 1215 1487 319
Fragments arranged by size:
1487 1215 319
BsrFI r'CCGG_y
Cuts at: 0 1262 1799 3021
Size: 1262 537 1222
Fragments arranged by size:
1262 1222 537
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1453 3021
Size: 1453 1568
BstEII G'GTnAC_C
Cuts at: 0 1818 3021
Size: 1818 1203
BstYI r'GATC_y
Cuts at: 0 324 578 1166 2150 3021
Size: 324 254 588 984 871
Fragments arranged by size:
984 871 588 324 254
Bsu36I CC'TnA_GG
Cuts at: 0 248 266 636 1833 3021
Size: 248 18 370 1197 1188
Fragments arranged by size:
1197 1188 370 248 18
BtgI C'CryG_G
Cuts at: 0 320 971 1236 1499 2913 3021
Size: 320 651 265 263 1414 108
Fragments arranged by size:
1414 651 320 265 263 108
BtsI GCAGTG_nn'
Cuts at: 0 525 1022 1155 1347 1553 1681 2574 3021
Size: 525 497 133 192 206 128 893 447
Fragments arranged by size:
893 525 497 447 206 192 133 128
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1197 1232 1971 2006 3021
Size: 1197 35 739 35 1015
Fragments arranged by size:
1197 1015 739 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1453 3021
Size: 1453 1568
EaeI y'GGCC_r
Cuts at: 0 425 522 1950 2748 3021
Size: 425 97 1428 798 273
Fragments arranged by size:
1428 798 425 273 97
EarI CTCTTCn'nnn_
Cuts at: 0 228 545 702 727 789 795 1884 2270
Size: 228 317 157 25 62 6 1089 386
Cuts at: 2270 2294 2312 2402 2585 2821 3021
Size: 24 18 90 183 236 200
Fragments arranged by size:
1089 386 317 236 228 200 183 157
90 62 25 24 18 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 491 3021
Size: 491 2530
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 443 708 776 887 1063 1385 1490
Size: 347 96 265 68 111 176 322 105
Cuts at: 1490 2198 2797 3021
Size: 708 599 224
Fragments arranged by size:
708 599 347 322 265 224 176 111
105 96 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 683 2689 3021
Size: 683 2006 332
Fragments arranged by size:
2006 683 332
EcoO109I rG'GnC_Cy
Cuts at: 0 52 308 336 414 1090 1196 1748 1932
Size: 52 256 28 78 676 106 552 184
Cuts at: 1932 1970 3021
Size: 38 1051
Fragments arranged by size:
1051 676 552 256 184 106 78 52
38 28
EcoRI G'AATT_C
Cuts at: 0 142 2070 3021
Size: 142 1928 951
Fragments arranged by size:
1928 951 142
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 834 3021
Size: 834 2187
FspI TGC'GCA
Cuts at: 0 120 3021
Size: 120 2901
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 324 2269 2301 3021
Size: 292 32 1945 32 720
Fragments arranged by size:
1945 720 292 32 32
MscI TGG'CCA
Cuts at: 0 427 524 1952 2750 3021
Size: 427 97 1428 798 271
Fragments arranged by size:
1428 798 427 271 97
MslI CAynn'nnrTG
Cuts at: 0 1187 1961 2659 3021
Size: 1187 774 698 362
Fragments arranged by size:
1187 774 698 362
MspA1I CmG'CkG
Cuts at: 0 18 397 573 936 1828 2056 2461 3021
Size: 18 379 176 363 892 228 405 560
Fragments arranged by size:
892 560 405 379 363 228 176 18
NcoI C'CATG_G
Cuts at: 0 2913 3021
Size: 2913 108
NdeI CA'TA_TG
Cuts at: 0 2873 3021
Size: 2873 148
NheI G'CTAG_C
Cuts at: 0 79 3021
Size: 79 2942
NspI r_CATG'y
Cuts at: 0 167 1288 1644 2128 3021
Size: 167 1121 356 484 893
Fragments arranged by size:
1121 893 484 356 167
PasI CC'CwG_GG
Cuts at: 0 508 1033 1585 3021
Size: 508 525 552 1436
Fragments arranged by size:
1436 552 525 508
PciI A'CATG_T
Cuts at: 0 1284 3021
Size: 1284 1737
PflMI CCAn_nnn'nTGG
Cuts at: 0 304 648 840 1054 3021
Size: 304 344 192 214 1967
Fragments arranged by size:
1967 344 304 214 192
PfoI T'CCnGG_A
Cuts at: 0 1506 3021
Size: 1506 1515
PpuMI rG'GwC_Cy
Cuts at: 0 52 308 1090 3021
Size: 52 256 782 1931
Fragments arranged by size:
1931 782 256 52
PspOMI G'GGCC_C
Cuts at: 0 336 1931 3021
Size: 336 1595 1090
Fragments arranged by size:
1595 1090 336
PstI C_TGCA'G
Cuts at: 0 569 613 1166 1778 2120 2690 2836 3021
Size: 569 44 553 612 342 570 146 185
Fragments arranged by size:
612 570 569 553 342 185 146 44
PvuII CAG'CTG
Cuts at: 0 936 1828 2056 2461 3021
Size: 936 892 228 405 560
Fragments arranged by size:
936 892 560 405 228
SanDI GG'GwC_CC
Cuts at: 0 1090 3021
Size: 1090 1931
SapI GCTCTTCn'nnn_
Cuts at: 0 545 2821 3021
Size: 545 2276 200
Fragments arranged by size:
2276 545 200
SbfI CC_TGCA'GG
Cuts at: 0 2690 3021
Size: 2690 331
SexAI A'CCwGG_T
Cuts at: 0 2891 3021
Size: 2891 130
SfcI C'TryA_G
Cuts at: 0 565 609 1162 1774 2116 2219 2686 2832
Size: 565 44 553 612 342 103 467 146
Cuts at: 2832 3021
Size: 189
Fragments arranged by size:
612 565 553 467 342 189 146 103
44
SmaI CCC'GGG
Cuts at: 0 2062 3021
Size: 2062 959
SmlI C'TyrA_G
Cuts at: 0 1015 2616 2848 2863 3021
Size: 1015 1601 232 15 158
Fragments arranged by size:
1601 1015 232 158 15
SphI G_CATG'C
Cuts at: 0 1644 3021
Size: 1644 1377
SrfI GCCC'GGGC
Cuts at: 0 2062 3021
Size: 2062 959
SspI AAT'ATT
Cuts at: 0 2928 3021
Size: 2928 93
StuI AGG'CCT
Cuts at: 0 817 1013 2734 2755 3021
Size: 817 196 1721 21 266
Fragments arranged by size:
1721 817 266 196 21
StyI C'CwwG_G
Cuts at: 0 1094 1742 2666 2801 2913 3021
Size: 1094 648 924 135 112 108
Fragments arranged by size:
1094 924 648 135 112 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 640 851 1319 3021
Size: 640 211 468 1702
Fragments arranged by size:
1702 640 468 211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1162 1194 1687 1719 3021
Size: 1162 32 493 32 1302
Fragments arranged by size:
1302 1162 493 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1327 3021
Size: 1327 1694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 435 756 978 1611 2664 3021
Size: 435 321 222 633 1053 357
Fragments arranged by size:
1053 633 435 357 321 222
XmaI C'CCGG_G
Cuts at: 0 2060 3021
Size: 2060 961
XmnI GAAnn'nnTTC
Cuts at: 0 2095 3021
Size: 2095 926
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbsI BbvCI BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstYI
Bsu36I BtgI BtsI CspCI DraIII EaeI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI Hin4I MscI
MslI MspA1I NcoI NdeI NheI NspI PasI PciI
PflMI PfoI PpuMI PspOMI PstI PvuII SanDI SapI
SbfI SexAI SfcI SmaI SmlI SphI SrfI SspI
StuI StyI TaqII TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BciVI BclI BfrBI BglI BglII BmgBI BsaI BsaAI
BsaBI BsaHI BsiEI BsiWI BsmBI BsrGI BssHII BssSI
BstBI BstXI BstZ17I BtgZI ClaI DraI DrdI EagI
EcoICRI EcoRV FseI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NgoMIV
NotI NruI NsiI PacI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SwaI
TatI XbaI XhoI ZraI