Restriction map for
pF1KA0365
(Linear) (Six-base) MAPSORT of: KIAA0365.seq Check: 6098 from: 1 to: 3246
KIAA0365.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1539 2817 3246
Size: 1539 1278 429
Fragments arranged by size:
1539 1278 429
AatII G_ACGT'C
Cuts at: 0 170 3246
Size: 170 3076
Acc65I G'GTAC_C
Cuts at: 0 2833 3246
Size: 2833 413
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 252 931 1330 1612 1915 2318 3246
Size: 88 164 679 399 282 303 403 928
Fragments arranged by size:
928 679 403 399 303 282 164 88
AflII C'TTAA_G
Cuts at: 0 126 3246
Size: 126 3120
AflIII A'CryG_T
Cuts at: 0 1785 3246
Size: 1785 1461
AleI CACnn'nnGTG
Cuts at: 0 2555 3246
Size: 2555 691
AlwNI CAG_nnn'CTG
Cuts at: 0 699 3024 3054 3246
Size: 699 2325 30 192
Fragments arranged by size:
2325 699 192 30
ApoI r'AATT_y
Cuts at: 0 334 409 1036 1156 1694 2482 3246
Size: 334 75 627 120 538 788 764
Fragments arranged by size:
788 764 627 538 334 120 75
AvaI C'yCGr_G
Cuts at: 0 353 1991 2963 3246
Size: 353 1638 972 283
Fragments arranged by size:
1638 972 353 283
AvrII C'CTAG_G
Cuts at: 0 675 3246
Size: 675 2571
BanI G'GyrC_C
Cuts at: 0 1762 2119 2833 3246
Size: 1762 357 714 413
Fragments arranged by size:
1762 714 413 357
BanII G_rGCy'C
Cuts at: 0 645 2045 2063 2590 2633 2662 3094 3246
Size: 645 1400 18 527 43 29 432 152
Fragments arranged by size:
1400 645 527 432 152 43 29 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 1243 1375 1559 2353 2630 3188 3246
Size: 1243 132 184 794 277 558 58
Fragments arranged by size:
1243 794 558 277 184 132 58
BbvCI CC'TCA_GC
Cuts at: 0 1956 3246
Size: 1956 1290
BglI GCCn_nnn'nGGC
Cuts at: 0 2774 3246
Size: 2774 472
BglII A'GATC_T
Cuts at: 0 120 3246
Size: 120 3126
BlpI GC'TnA_GC
Cuts at: 0 2648 3246
Size: 2648 598
Bme1580I G_kGCm'C
Cuts at: 0 802 1765 3246
Size: 802 963 1481
Fragments arranged by size:
1481 963 802
BmgBI CAC'GTC
Cuts at: 0 576 2531 3246
Size: 576 1955 715
Fragments arranged by size:
1955 715 576
BmrI ACTGGGnnnn_n'
Cuts at: 0 2215 3246
Size: 2215 1031
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 615 2926 3246
Size: 615 2311 320
Fragments arranged by size:
2311 615 320
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1609 1894 2135 2674 2747 2822 3246
Size: 1609 285 241 539 73 75 424
Fragments arranged by size:
1609 539 424 285 241 75 73
Bpu10I CC'TnA_GC
Cuts at: 0 607 710 1956 2387 3246
Size: 607 103 1246 431 859
Fragments arranged by size:
1246 859 607 431 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 443 1993 2039 3246
Size: 101 342 1550 46 1207
Fragments arranged by size:
1550 1207 342 101 46
BsaHI Gr'CG_yC
Cuts at: 0 167 3246
Size: 167 3079
BsaWI w'CCGG_w
Cuts at: 0 374 3246
Size: 374 2872
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2301 2331 2520 2550 3246
Size: 2301 30 189 30 696
Fragments arranged by size:
2301 696 189 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1675 1678 1949 2035 2094 2225 2692 2695
Size: 1675 3 271 86 59 131 467 3
Cuts at: 2695 2725 2817 3246
Size: 30 92 429
Fragments arranged by size:
1675 467 429 271 131 92 86 59
30 3 3
BseYI C'CCAG_C
Cuts at: 0 1736 2245 3014 3246
Size: 1736 509 769 232
Fragments arranged by size:
1736 769 509 232
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1988 3246
Size: 1988 1258
BsiEI CG_ry'CG
Cuts at: 0 2142 3246
Size: 2142 1104
BsiHKAI G_wGCw'C
Cuts at: 0 645 754 1130 2055 2808 3176 3246
Size: 645 109 376 925 753 368 70
Fragments arranged by size:
925 753 645 376 368 109 70
Bsp1286I G_dGCh'C
Cuts at: 0 645 754 802 1130 1765 2045 2055 2063
Size: 645 109 48 328 635 280 10 8
Cuts at: 2063 2590 2633 2662 2808 3094 3176 3246
Size: 527 43 29 146 286 82 70
Fragments arranged by size:
645 635 527 328 286 280 146 109
82 70 48 43 29 10 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1539 2817 2845 3246
Size: 1539 1278 28 401
Fragments arranged by size:
1539 1278 401 28
BsrBI CCG'CTC
Cuts at: 0 2722 3246
Size: 2722 524
BsrDI GCAATG_nn'
Cuts at: 0 1347 3246
Size: 1347 1899
BsrFI r'CCGG_y
Cuts at: 0 1301 2141 3117 3246
Size: 1301 840 976 129
Fragments arranged by size:
1301 976 840 129
BsrGI T'GTAC_A
Cuts at: 0 3221 3246
Size: 3221 25
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1966 3246
Size: 1966 1280
BstBI TT'CG_AA
Cuts at: 0 1550 2943 3246
Size: 1550 1393 303
Fragments arranged by size:
1550 1393 303
BstXI CCAn_nnnn'nTGG
Cuts at: 0 178 367 3003 3246
Size: 178 189 2636 243
Fragments arranged by size:
2636 243 189 178
BstYI r'GATC_y
Cuts at: 0 120 142 199 327 520 890 906 3246
Size: 120 22 57 128 193 370 16 2340
Fragments arranged by size:
2340 370 193 128 120 57 22 16
Bsu36I CC'TnA_GG
Cuts at: 0 2115 2237 2259 2675 2829 3246
Size: 2115 122 22 416 154 417
Fragments arranged by size:
2115 417 416 154 122 22
BtgI C'CryG_G
Cuts at: 0 420 2026 2136 2570 2591 2638 3081 3246
Size: 420 1606 110 434 21 47 443 165
Fragments arranged by size:
1606 443 434 420 165 110 47 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 191 3246
Size: 191 3055
BtsI GCAGTG_nn'
Cuts at: 0 1976 3246
Size: 1976 1270
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 300 335 1548 1583 3246
Size: 300 35 1213 35 1663
Fragments arranged by size:
1663 1213 300 35 35
DraI TTT'AAA
Cuts at: 0 111 1163 1208 3246
Size: 111 1052 45 2038
Fragments arranged by size:
2038 1052 111 45
EaeI y'GGCC_r
Cuts at: 0 321 369 444 2139 3246
Size: 321 48 75 1695 1107
Fragments arranged by size:
1695 1107 321 75 48
EagI C'GGCC_G
Cuts at: 0 2139 3246
Size: 2139 1107
EarI CTCTTCn'nnn_
Cuts at: 0 107 222 1306 1476 1829 2319 2845 3246
Size: 107 115 1084 170 353 490 526 401
Fragments arranged by size:
1084 526 490 401 353 170 115 107
EcoICRI GAG'CTC
Cuts at: 0 643 3246
Size: 643 2603
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 252 931 1330 1609 1612 1894 1915
Size: 88 164 679 399 279 3 282 21
Cuts at: 1915 2135 2318 2674 2747 2822 3246
Size: 220 183 356 73 75 424
Fragments arranged by size:
679 424 399 356 282 279 220 183
164 88 75 73 21 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 679 2161 2955 3100 3246
Size: 679 1482 794 145 146
Fragments arranged by size:
1482 794 679 146 145
EcoO109I rG'GnC_Cy
Cuts at: 0 259 696 1836 2096 2241 2715 3246
Size: 259 437 1140 260 145 474 531
Fragments arranged by size:
1140 531 474 437 260 259 145
EcoRV GAT'ATC
Cuts at: 0 64 870 3246
Size: 64 806 2376
Fragments arranged by size:
2376 806 64
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 299 960 1321 3246
Size: 299 661 361 1925
Fragments arranged by size:
1925 661 361 299
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 176 208 505 537 1607 1639 2276 2301
Size: 176 32 297 32 1070 32 637 25
Cuts at: 2301 2308 2333 2387 2419 2877 2892 2909 2924
Size: 7 25 54 32 458 15 17 15
Cuts at: 2924 3246
Size: 322
Fragments arranged by size:
1070 637 458 322 297 176 54 32
32 32 32 25 25 17 15 15
7
HincII GTy'rAC
Cuts at: 0 657 3246
Size: 657 2589
KpnI G_GTAC'C
Cuts at: 0 2837 3246
Size: 2837 409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2205 3225 3246
Size: 2205 1020 21
Fragments arranged by size:
2205 1020 21
MscI TGG'CCA
Cuts at: 0 371 446 3246
Size: 371 75 2800
Fragments arranged by size:
2800 371 75
MslI CAynn'nnrTG
Cuts at: 0 71 176 1107 2341 2555 3246
Size: 71 105 931 1234 214 691
Fragments arranged by size:
1234 931 691 214 105 71
MspA1I CmG'CkG
Cuts at: 0 1613 1914 2034 2640 3246
Size: 1613 301 120 606 606
Fragments arranged by size:
1613 606 606 301 120
NaeI GCC'GGC
Cuts at: 0 2143 3246
Size: 2143 1103
NcoI C'CATG_G
Cuts at: 0 3081 3246
Size: 3081 165
NgoMIV G'CCGG_C
Cuts at: 0 2141 3246
Size: 2141 1105
NspI r_CATG'y
Cuts at: 0 1215 3246
Size: 1215 2031
PasI CC'CwG_GG
Cuts at: 0 843 856 3246
Size: 843 13 2390
Fragments arranged by size:
2390 843 13
PflMI CCAn_nnn'nTGG
Cuts at: 0 3087 3246
Size: 3087 159
PfoI T'CCnGG_A
Cuts at: 0 2724 2915 3246
Size: 2724 191 331
Fragments arranged by size:
2724 331 191
PpuMI rG'GwC_Cy
Cuts at: 0 1836 2096 2241 3246
Size: 1836 260 145 1005
Fragments arranged by size:
1836 1005 260 145
PstI C_TGCA'G
Cuts at: 0 504 2364 3059 3246
Size: 504 1860 695 187
Fragments arranged by size:
1860 695 504 187
SacI G_AGCT'C
Cuts at: 0 645 3246
Size: 645 2601
SacII CC_GC'GG
Cuts at: 0 2641 3246
Size: 2641 605
SanDI GG'GwC_CC
Cuts at: 0 2096 3246
Size: 2096 1150
SexAI A'CCwGG_T
Cuts at: 0 359 3246
Size: 359 2887
SfcI C'TryA_G
Cuts at: 0 203 500 2360 3055 3246
Size: 203 297 1860 695 191
Fragments arranged by size:
1860 695 297 203 191
SmaI CCC'GGG
Cuts at: 0 1993 3246
Size: 1993 1253
SmlI C'TyrA_G
Cuts at: 0 116 126 458 2008 2054 3246
Size: 116 10 332 1550 46 1192
Fragments arranged by size:
1550 1192 332 116 46 10
SspI AAT'ATT
Cuts at: 0 1885 3246
Size: 1885 1361
StuI AGG'CCT
Cuts at: 0 236 1597 2156 2824 2961 3246
Size: 236 1361 559 668 137 285
Fragments arranged by size:
1361 668 559 285 236 137
StyI C'CwwG_G
Cuts at: 0 675 1463 1703 2201 2996 3081 3246
Size: 675 788 240 498 795 85 165
Fragments arranged by size:
795 788 675 498 240 165 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1200 3043 3145 3246
Size: 1200 1843 102 101
Fragments arranged by size:
1843 1200 102 101
TatI w'GTAC_w
Cuts at: 0 1280 1577 1985 3221 3246
Size: 1280 297 408 1236 25
Fragments arranged by size:
1280 1236 408 297 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 813 845 2520 2552 3246
Size: 813 32 1675 32 694
Fragments arranged by size:
1675 813 694 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 361 3246
Size: 361 2885
XbaI T'CTAG_A
Cuts at: 0 214 3246
Size: 214 3032
XmaI C'CCGG_G
Cuts at: 0 1991 3246
Size: 1991 1255
XmnI GAAnn'nnTTC
Cuts at: 0 101 2939 3246
Size: 101 2838 307
Fragments arranged by size:
2838 307 101
ZraI GAC'GTC
Cuts at: 0 168 3246
Size: 168 3078
Enzymes that do cut:
AarI AatII Acc65I AcuI AflII AflIII AleI AlwNI
ApoI AvaI AvrII BanI BanII BbsI BbvCI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI Hin4I HincII KpnI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI
PpuMI PstI SacI SacII SanDI SexAI SfcI SmaI
SmlI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AgeI AhdI AlfI AloI ApaI
ApaLI AscI AseI AsiSI BaeI BamHI BbeI BcgI
BciVI BclI BfrBI BmtI BsaI BsaAI BsaBI BsiWI
BsmI BsmBI BspEI BspHI BssHII BssSI BstEII BstZ17I
ClaI DraIII DrdI EciI EcoRI FseI FspI FspAI
HaeII HindIII HpaI KasI MfeI MluI NarI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SalI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SwaI XcmI XhoI