Restriction map for
pF1KA0370
(Linear) (Six-base) MAPSORT of: KIAA0370.seq Check: 1633 from: 1 to: 3375
KIAA0370.orf
With 182 enzymes: SgfI *
July 23, 2007 10:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2679 3375
Size: 2679 696
AatII G_ACGT'C
Cuts at: 0 357 3375
Size: 357 3018
AccI GT'mk_AC
Cuts at: 0 3167 3375
Size: 3167 208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 443 753 1531 1580 2314 2698 3375
Size: 29 414 310 778 49 734 384 677
Fragments arranged by size:
778 734 677 414 384 310 49 29
AflIII A'CryG_T
Cuts at: 0 2600 3375
Size: 2600 775
AleI CACnn'nnGTG
Cuts at: 0 1366 1756 2059 3375
Size: 1366 390 303 1316
Fragments arranged by size:
1366 1316 390 303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1929 3375
Size: 1929 1446
AlwNI CAG_nnn'CTG
Cuts at: 0 1872 2439 3033 3099 3338 3375
Size: 1872 567 594 66 239 37
Fragments arranged by size:
1872 594 567 239 66 37
ApaLI G'TGCA_C
Cuts at: 0 3301 3375
Size: 3301 74
ApoI r'AATT_y
Cuts at: 0 49 982 3258 3375
Size: 49 933 2276 117
Fragments arranged by size:
2276 933 117 49
AvaI C'yCGr_G
Cuts at: 0 900 2276 2397 3375
Size: 900 1376 121 978
Fragments arranged by size:
1376 978 900 121
BanII G_rGCy'C
Cuts at: 0 1088 1508 1675 2175 2822 2924 3375
Size: 1088 420 167 500 647 102 451
Fragments arranged by size:
1088 647 500 451 420 167 102
BbsI GAAGACnn'nnnn_
Cuts at: 0 457 3375
Size: 457 2918
BbvCI CC'TCA_GC
Cuts at: 0 2900 3375
Size: 2900 475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2408 2442 3375
Size: 2408 34 933
Fragments arranged by size:
2408 933 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2777 3375
Size: 2777 598
BclI T'GATC_A
Cuts at: 0 212 440 2078 3375
Size: 212 228 1638 1297
Fragments arranged by size:
1638 1297 228 212
BglII A'GATC_T
Cuts at: 0 3047 3375
Size: 3047 328
BlpI GC'TnA_GC
Cuts at: 0 2515 3375
Size: 2515 860
Bme1580I G_kGCm'C
Cuts at: 0 3305 3375
Size: 3305 70
BmrI ACTGGGnnnn_n'
Cuts at: 0 668 1164 2294 2919 3375
Size: 668 496 1130 625 456
Fragments arranged by size:
1130 668 625 496 456
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1182 2412 2805 3375
Size: 1182 1230 393 570
Fragments arranged by size:
1230 1182 570 393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 153 1429 2868 2950 3076 3375
Size: 141 12 1276 1439 82 126 299
Fragments arranged by size:
1439 1276 299 141 126 82 12
Bpu10I CC'TnA_GC
Cuts at: 0 2900 3375
Size: 2900 475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1689 3375
Size: 1689 1686
BsaI GGTCTCn'nnnn_
Cuts at: 0 2049 2463 3375
Size: 2049 414 912
Fragments arranged by size:
2049 912 414
BsaBI GATnn'nnATC
Cuts at: 0 256 1439 2083 3139 3375
Size: 256 1183 644 1056 236
Fragments arranged by size:
1183 1056 644 256 236
BsaHI Gr'CG_yC
Cuts at: 0 354 868 3375
Size: 354 514 2507
Fragments arranged by size:
2507 514 354
BsaWI w'CCGG_w
Cuts at: 0 317 2828 3053 3375
Size: 317 2511 225 322
Fragments arranged by size:
2511 322 317 225
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 139 169 712 742 1510 1540 1657 1687
Size: 139 30 543 30 768 30 117 30
Cuts at: 1687 2749 2779 3375
Size: 1062 30 596
Fragments arranged by size:
1062 768 596 543 139 117 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 523 1003 1426 2917 3145 3375
Size: 109 414 480 423 1491 228 230
Fragments arranged by size:
1491 480 423 414 230 228 109
BseYI C'CCAG_C
Cuts at: 0 116 921 1403 2734 3249 3375
Size: 116 805 482 1331 515 126
Fragments arranged by size:
1331 805 515 482 126 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 1633 1715 2116 2252 3375
Size: 559 1074 82 401 136 1123
Fragments arranged by size:
1123 1074 559 401 136 82
BsiEI CG_ry'CG
Cuts at: 0 918 3375
Size: 918 2457
BsiHKAI G_wGCw'C
Cuts at: 0 1088 1355 1508 1675 2822 3305 3375
Size: 1088 267 153 167 1147 483 70
Fragments arranged by size:
1147 1088 483 267 167 153 70
Bsp1286I G_dGCh'C
Cuts at: 0 1088 1355 1508 1675 2175 2822 2924 3305
Size: 1088 267 153 167 500 647 102 381
Cuts at: 3305 3375
Size: 70
Fragments arranged by size:
1088 647 500 381 267 167 153 102
70
BspEI T'CCGG_A
Cuts at: 0 317 2828 3053 3375
Size: 317 2511 225 322
Fragments arranged by size:
2511 322 317 225
BspHI T'CATG_A
Cuts at: 0 1230 3375
Size: 1230 2145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2679 3287 3329 3375
Size: 2679 608 42 46
Fragments arranged by size:
2679 608 46 42
BsrDI GCAATG_nn'
Cuts at: 0 2774 3375
Size: 2774 601
BsrFI r'CCGG_y
Cuts at: 0 917 1640 1928 3375
Size: 917 723 288 1447
Fragments arranged by size:
1447 917 723 288
BsrGI T'GTAC_A
Cuts at: 0 64 185 1805 3375
Size: 64 121 1620 1570
Fragments arranged by size:
1620 1570 121 64
BssSI C'ACGA_G
Cuts at: 0 2638 3375
Size: 2638 737
BstAPI GCAn_nnn'nTGC
Cuts at: 0 546 2654 3375
Size: 546 2108 721
Fragments arranged by size:
2108 721 546
BstXI CCAn_nnnn'nTGG
Cuts at: 0 864 1500 2874 3375
Size: 864 636 1374 501
Fragments arranged by size:
1374 864 636 501
BstYI r'GATC_y
Cuts at: 0 1789 2681 3047 3375
Size: 1789 892 366 328
Fragments arranged by size:
1789 892 366 328
Bsu36I CC'TnA_GG
Cuts at: 0 630 3375
Size: 630 2745
BtgI C'CryG_G
Cuts at: 0 1688 1970 2197 3375
Size: 1688 282 227 1178
Fragments arranged by size:
1688 1178 282 227
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2727 3126 3375
Size: 2727 399 249
Fragments arranged by size:
2727 399 249
BtsI GCAGTG_nn'
Cuts at: 0 1650 1923 2099 2233 3375
Size: 1650 273 176 134 1142
Fragments arranged by size:
1650 1142 273 176 134
DraI TTT'AAA
Cuts at: 0 2988 3375
Size: 2988 387
DraIII CAC_nnn'GTG
Cuts at: 0 1065 3375
Size: 1065 2310
EaeI y'GGCC_r
Cuts at: 0 915 932 1490 1700 2051 2200 2876 3375
Size: 915 17 558 210 351 149 676 499
Fragments arranged by size:
915 676 558 499 351 210 149 17
EagI C'GGCC_G
Cuts at: 0 915 3375
Size: 915 2460
EarI CTCTTCn'nnn_
Cuts at: 0 458 772 1063 1076 3004 3375
Size: 458 314 291 13 1928 371
Fragments arranged by size:
1928 458 371 314 291 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1642 3270 3375
Size: 1642 1628 105
Fragments arranged by size:
1642 1628 105
EcoICRI GAG'CTC
Cuts at: 0 1086 1506 1673 2820 3375
Size: 1086 420 167 1147 555
Fragments arranged by size:
1147 1086 555 420 167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 141 153 443 753 1429 1531 1580
Size: 29 112 12 290 310 676 102 49
Cuts at: 1580 2314 2698 2868 2950 3076 3375
Size: 734 384 170 82 126 299
Fragments arranged by size:
734 676 384 310 299 290 170 126
112 102 82 49 29 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 21 1681 2638 3375
Size: 21 1660 957 737
Fragments arranged by size:
1660 957 737 21
EcoO109I rG'GnC_Cy
Cuts at: 0 234 486 927 3096 3276 3375
Size: 234 252 441 2169 180 99
Fragments arranged by size:
2169 441 252 234 180 99
EcoRV GAT'ATC
Cuts at: 0 1257 3375
Size: 1257 2118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3066 3375
Size: 3066 309
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1657 1689 2823 2855 3268 3300 3375
Size: 1657 32 1134 32 413 32 75
Fragments arranged by size:
1657 1134 413 75 32 32 32
HincII GTy'rAC
Cuts at: 0 876 3168 3315 3375
Size: 876 2292 147 60
Fragments arranged by size:
2292 876 147 60
HpaI GTT'AAC
Cuts at: 0 876 3375
Size: 876 2499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 1397 3375
Size: 754 643 1978
Fragments arranged by size:
1978 754 643
MscI TGG'CCA
Cuts at: 0 934 1492 2053 2202 2878 3375
Size: 934 558 561 149 676 497
Fragments arranged by size:
934 676 561 558 497 149
MslI CAynn'nnrTG
Cuts at: 0 1366 1756 1855 2059 3375
Size: 1366 390 99 204 1316
Fragments arranged by size:
1366 1316 390 204 99
MspA1I CmG'CkG
Cuts at: 0 1407 1461 3215 3375
Size: 1407 54 1754 160
Fragments arranged by size:
1754 1407 160 54
NaeI GCC'GGC
Cuts at: 0 919 1642 3375
Size: 919 723 1733
Fragments arranged by size:
1733 919 723
NcoI C'CATG_G
Cuts at: 0 1970 3375
Size: 1970 1405
NgoMIV G'CCGG_C
Cuts at: 0 917 1640 3375
Size: 917 723 1735
Fragments arranged by size:
1735 917 723
NspI r_CATG'y
Cuts at: 0 1864 2604 3375
Size: 1864 740 771
Fragments arranged by size:
1864 771 740
PciI A'CATG_T
Cuts at: 0 2600 3375
Size: 2600 775
PflMI CCAn_nnn'nTGG
Cuts at: 0 2040 2160 3099 3375
Size: 2040 120 939 276
Fragments arranged by size:
2040 939 276 120
PfoI T'CCnGG_A
Cuts at: 0 2699 3375
Size: 2699 676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 660 692 883 915 2911 2943 3375
Size: 660 32 191 32 1996 32 432
Fragments arranged by size:
1996 660 432 191 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 234 486 927 3096 3276 3375
Size: 234 252 441 2169 180 99
Fragments arranged by size:
2169 441 252 234 180 99
PstI C_TGCA'G
Cuts at: 0 1034 1685 1886 2094 2444 2690 3128 3375
Size: 1034 651 201 208 350 246 438 247
Fragments arranged by size:
1034 651 438 350 247 246 208 201
PvuII CAG'CTG
Cuts at: 0 1407 3215 3375
Size: 1407 1808 160
Fragments arranged by size:
1808 1407 160
SacI G_AGCT'C
Cuts at: 0 1088 1508 1675 2822 3375
Size: 1088 420 167 1147 553
Fragments arranged by size:
1147 1088 553 420 167
SalI G'TCGA_C
Cuts at: 0 3166 3375
Size: 3166 209
SanDI GG'GwC_CC
Cuts at: 0 234 3375
Size: 234 3141
SapI GCTCTTCn'nnn_
Cuts at: 0 458 1063 1076 3375
Size: 458 605 13 2299
Fragments arranged by size:
2299 605 458 13
SbfI CC_TGCA'GG
Cuts at: 0 2690 3375
Size: 2690 685
ScaI AGT'ACT
Cuts at: 0 1708 3375
Size: 1708 1667
SexAI A'CCwGG_T
Cuts at: 0 533 3375
Size: 533 2842
SfcI C'TryA_G
Cuts at: 0 1030 1681 1882 2090 2440 2686 3124 3330
Size: 1030 651 201 208 350 246 438 206
Cuts at: 3330 3375
Size: 45
Fragments arranged by size:
1030 651 438 350 246 208 206 201
45
SmaI CCC'GGG
Cuts at: 0 2278 3375
Size: 2278 1097
SmlI C'TyrA_G
Cuts at: 0 1668 3375
Size: 1668 1707
SphI G_CATG'C
Cuts at: 0 1864 3375
Size: 1864 1511
StuI AGG'CCT
Cuts at: 0 2543 3375
Size: 2543 832
StyI C'CwwG_G
Cuts at: 0 432 1043 1970 2000 3375
Size: 432 611 927 30 1375
Fragments arranged by size:
1375 927 611 432 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2548 3307 3375
Size: 2548 759 68
Fragments arranged by size:
2548 759 68
TatI w'GTAC_w
Cuts at: 0 64 146 185 1208 1706 1805 2550 3375
Size: 64 82 39 1023 498 99 745 825
Fragments arranged by size:
1023 825 745 498 99 82 64 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1510 1542 3375
Size: 1510 32 1833
Fragments arranged by size:
1833 1510 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 282 714 3153 3375
Size: 282 432 2439 222
Fragments arranged by size:
2439 432 282 222
XmaI C'CCGG_G
Cuts at: 0 2276 3375
Size: 2276 1099
XmnI GAAnn'nnTTC
Cuts at: 0 53 1322 2104 2450 3049 3375
Size: 53 1269 782 346 599 326
Fragments arranged by size:
1269 782 599 346 326 53
ZraI GAC'GTC
Cuts at: 0 355 3375
Size: 355 3020
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AvaI BanII BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I HincII
HpaI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PciI PflMI PfoI PpiI PpuMI PstI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AloI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BfrBI BglI BmgBI BmtI BsaAI BsiWI BsmI BsmBI
BsrBI BssHII BstBI BstEII BstZ17I ClaI CspCI DrdI
EcoRI FseI FspI FspAI HaeII HindIII KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
PacI PasI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI