Restriction map for
pF1KA0371
(Linear) (Six-base) MAPSORT of: KIAA0371.seq Check: 6144 from: 1 to: 3594
KIAA0371.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 369 3303 3594
Size: 369 2934 291
Fragments arranged by size:
2934 369 291
Acc65I G'GTAC_C
Cuts at: 0 2461 3594
Size: 2461 1133
AclI AA'CG_TT
Cuts at: 0 1413 3594
Size: 1413 2181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 352 881 1441 2636 3175 3317 3594
Size: 73 279 529 560 1195 539 142 277
Fragments arranged by size:
1195 560 539 529 279 277 142 73
AfeI AGC'GCT
Cuts at: 0 1184 3594
Size: 1184 2410
AflIII A'CryG_T
Cuts at: 0 1597 2540 2731 3594
Size: 1597 943 191 863
Fragments arranged by size:
1597 943 863 191
AgeI A'CCGG_T
Cuts at: 0 1373 3594
Size: 1373 2221
AleI CACnn'nnGTG
Cuts at: 0 1753 2011 3594
Size: 1753 258 1583
Fragments arranged by size:
1753 1583 258
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1120 3594
Size: 1120 2474
AlwNI CAG_nnn'CTG
Cuts at: 0 641 967 1083 2800 3090 3153 3180 3594
Size: 641 326 116 1717 290 63 27 414
Fragments arranged by size:
1717 641 414 326 290 116 63 27
ApaI G_GGCC'C
Cuts at: 0 2579 3594
Size: 2579 1015
ApaLI G'TGCA_C
Cuts at: 0 1095 1769 3594
Size: 1095 674 1825
Fragments arranged by size:
1825 1095 674
ApoI r'AATT_y
Cuts at: 0 1363 3594
Size: 1363 2231
AvaI C'yCGr_G
Cuts at: 0 833 3594
Size: 833 2761
AvrII C'CTAG_G
Cuts at: 0 1995 3594
Size: 1995 1599
BamHI G'GATC_C
Cuts at: 0 1974 3594
Size: 1974 1620
BanI G'GyrC_C
Cuts at: 0 671 1789 2023 2461 2788 3594
Size: 671 1118 234 438 327 806
Fragments arranged by size:
1118 806 671 438 327 234
BanII G_rGCy'C
Cuts at: 0 584 2315 2579 3594
Size: 584 1731 264 1015
Fragments arranged by size:
1731 1015 584 264
BbsI GAAGACnn'nnnn_
Cuts at: 0 2194 3594
Size: 2194 1400
BbvCI CC'TCA_GC
Cuts at: 0 2054 2202 3594
Size: 2054 148 1392
Fragments arranged by size:
2054 1392 148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1750 1784 3594
Size: 1750 34 1810
Fragments arranged by size:
1810 1750 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1961 2125 3594
Size: 1961 164 1469
Fragments arranged by size:
1961 1469 164
BglII A'GATC_T
Cuts at: 0 47 375 2593 3594
Size: 47 328 2218 1001
Fragments arranged by size:
2218 1001 328 47
BlpI GC'TnA_GC
Cuts at: 0 1280 1948 3594
Size: 1280 668 1646
Fragments arranged by size:
1646 1280 668
Bme1580I G_kGCm'C
Cuts at: 0 1099 1773 2579 2793 3594
Size: 1099 674 806 214 801
Fragments arranged by size:
1099 806 801 674 214
BmgBI CAC'GTC
Cuts at: 0 867 3594
Size: 867 2727
BmrI ACTGGGnnnn_n'
Cuts at: 0 1414 2242 2431 3494 3594
Size: 1414 828 189 1063 100
Fragments arranged by size:
1414 1063 828 189 100
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2291 3309 3594
Size: 2291 1018 285
Fragments arranged by size:
2291 1018 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 546 1988 2320 2386 2827 3187 3432 3531
Size: 546 1442 332 66 441 360 245 99
Cuts at: 3531 3594
Size: 63
Fragments arranged by size:
1442 546 441 360 332 245 99 66
63
Bpu10I CC'TnA_GC
Cuts at: 0 1813 2054 2202 3594
Size: 1813 241 148 1392
Fragments arranged by size:
1813 1392 241 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 560 2940 3594
Size: 46 514 2380 654
Fragments arranged by size:
2380 654 514 46
BsaI GGTCTCn'nnnn_
Cuts at: 0 437 848 2500 3594
Size: 437 411 1652 1094
Fragments arranged by size:
1652 1094 437 411
BsaBI GATnn'nnATC
Cuts at: 0 83 752 1211 3594
Size: 83 669 459 2383
Fragments arranged by size:
2383 669 459 83
BsaWI w'CCGG_w
Cuts at: 0 1373 3594
Size: 1373 2221
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3160 3190 3594
Size: 3160 30 404
Fragments arranged by size:
3160 404 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1004 1083 2110 2265 2314 3594
Size: 1004 79 1027 155 49 1280
Fragments arranged by size:
1280 1027 1004 155 79 49
BseYI C'CCAG_C
Cuts at: 0 1247 1779 1903 2644 3305 3478 3594
Size: 1247 532 124 741 661 173 116
Fragments arranged by size:
1247 741 661 532 173 124 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 1534 1739 3594
Size: 471 1063 205 1855
Fragments arranged by size:
1855 1063 471 205
BsiEI CG_ry'CG
Cuts at: 0 799 1222 3594
Size: 799 423 2372
Fragments arranged by size:
2372 799 423
BsiHKAI G_wGCw'C
Cuts at: 0 124 584 1099 1773 1931 3594
Size: 124 460 515 674 158 1663
Fragments arranged by size:
1663 674 515 460 158 124
BsmI GAATG_Cn'
Cuts at: 0 244 1006 1495 3594
Size: 244 762 489 2099
Fragments arranged by size:
2099 762 489 244
Bsp1286I G_dGCh'C
Cuts at: 0 124 584 1099 1773 1931 2315 2579 2793
Size: 124 460 515 674 158 384 264 214
Cuts at: 2793 3594
Size: 801
Fragments arranged by size:
801 674 515 460 384 264 214 158
124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 369 1736 2124 3303 3594
Size: 369 1367 388 1179 291
Fragments arranged by size:
1367 1179 388 369 291
BsrDI GCAATG_nn'
Cuts at: 0 155 687 692 2790 2868 3575 3594
Size: 155 532 5 2098 78 707 19
Fragments arranged by size:
2098 707 532 155 78 19 5
BsrFI r'CCGG_y
Cuts at: 0 1221 1373 1817 1886 1931 3594
Size: 1221 152 444 69 45 1663
Fragments arranged by size:
1663 1221 444 152 69 45
BsrGI T'GTAC_A
Cuts at: 0 3065 3594
Size: 3065 529
BssSI C'ACGA_G
Cuts at: 0 1330 3594
Size: 1330 2264
BstAPI GCAn_nnn'nTGC
Cuts at: 0 278 397 3518 3594
Size: 278 119 3121 76
Fragments arranged by size:
3121 278 119 76
BstBI TT'CG_AA
Cuts at: 0 2388 3594
Size: 2388 1206
BstXI CCAn_nnnn'nTGG
Cuts at: 0 682 1755 3594
Size: 682 1073 1839
Fragments arranged by size:
1839 1073 682
BstYI r'GATC_y
Cuts at: 0 47 375 1110 1974 2593 3594
Size: 47 328 735 864 619 1001
Fragments arranged by size:
1001 864 735 619 328 47
Bsu36I CC'TnA_GG
Cuts at: 0 2195 3594
Size: 2195 1399
BtgI C'CryG_G
Cuts at: 0 2636 3594
Size: 2636 958
BtsI GCAGTG_nn'
Cuts at: 0 322 1233 1438 1727 3369 3417 3594
Size: 322 911 205 289 1642 48 177
Fragments arranged by size:
1642 911 322 289 205 177 48
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 543 578 3594
Size: 543 35 3016
Fragments arranged by size:
3016 543 35
DraI TTT'AAA
Cuts at: 0 483 3594
Size: 483 3111
EaeI y'GGCC_r
Cuts at: 0 1219 1356 1880 3362 3594
Size: 1219 137 524 1482 232
Fragments arranged by size:
1482 1219 524 232 137
EagI C'GGCC_G
Cuts at: 0 1219 3594
Size: 1219 2375
EarI CTCTTCn'nnn_
Cuts at: 0 2 328 385 2390 3151 3594
Size: 2 326 57 2005 761 443
Fragments arranged by size:
2005 761 443 326 57 2
EcoICRI GAG'CTC
Cuts at: 0 582 3594
Size: 582 3012
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 352 546 881 1441 1988 2320 2386
Size: 73 279 194 335 560 547 332 66
Cuts at: 2386 2636 2827 3175 3187 3317 3432 3531 3594
Size: 250 191 348 12 130 115 99 63
Fragments arranged by size:
560 547 348 335 332 279 250 194
191 130 115 99 73 66 63 12
EcoO109I rG'GnC_Cy
Cuts at: 0 638 1326 2157 2340 2370 2576 2675 2810
Size: 638 688 831 183 30 206 99 135
Cuts at: 2810 3594
Size: 784
Fragments arranged by size:
831 784 688 638 206 183 135 99
30
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1516 2443 2791 3594
Size: 1516 927 348 803
Fragments arranged by size:
1516 927 803 348
HaeII r_GCGC'y
Cuts at: 0 1186 1893 1938 3090 3594
Size: 1186 707 45 1152 504
Fragments arranged by size:
1186 1152 707 504 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 167 199 367 399 781 813 937 969
Size: 167 32 168 32 382 32 124 32
Cuts at: 969 1095 1127 2241 2273 3043 3075 3594
Size: 126 32 1114 32 770 32 519
Fragments arranged by size:
1114 770 519 382 168 167 126 124
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 818 3594
Size: 818 2776
HindIII A'AGCT_T
Cuts at: 0 776 931 3594
Size: 776 155 2663
Fragments arranged by size:
2663 776 155
KpnI G_GTAC'C
Cuts at: 0 2465 3594
Size: 2465 1129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 560 945 3105 3594
Size: 378 182 385 2160 489
Fragments arranged by size:
2160 489 385 378 182
MscI TGG'CCA
Cuts at: 0 1358 1882 3594
Size: 1358 524 1712
Fragments arranged by size:
1712 1358 524
MslI CAynn'nnrTG
Cuts at: 0 1102 1548 1753 2011 2472 2545 3061 3200
Size: 1102 446 205 258 461 73 516 139
Cuts at: 3200 3334 3374 3594
Size: 134 40 220
Fragments arranged by size:
1102 516 461 446 258 220 205 139
134 73 40
MspA1I CmG'CkG
Cuts at: 0 66 701 964 1218 1766 2919 3305 3594
Size: 66 635 263 254 548 1153 386 289
Fragments arranged by size:
1153 635 548 386 289 263 254 66
NaeI GCC'GGC
Cuts at: 0 1819 1888 3594
Size: 1819 69 1706
Fragments arranged by size:
1819 1706 69
NcoI C'CATG_G
Cuts at: 0 2636 3594
Size: 2636 958
NgoMIV G'CCGG_C
Cuts at: 0 1817 1886 3594
Size: 1817 69 1708
Fragments arranged by size:
1817 1708 69
NspI r_CATG'y
Cuts at: 0 469 1201 1601 2544 3060 3594
Size: 469 732 400 943 516 534
Fragments arranged by size:
943 732 534 516 469 400
PasI CC'CwG_GG
Cuts at: 0 2348 3594
Size: 2348 1246
PciI A'CATG_T
Cuts at: 0 1597 2540 3594
Size: 1597 943 1054
Fragments arranged by size:
1597 1054 943
PflMI CCAn_nnn'nTGG
Cuts at: 0 1269 3594
Size: 1269 2325
PfoI T'CCnGG_A
Cuts at: 0 71 640 1113 3146 3594
Size: 71 569 473 2033 448
Fragments arranged by size:
2033 569 473 448 71
PpuMI rG'GwC_Cy
Cuts at: 0 638 1326 2157 2340 2370 3594
Size: 638 688 831 183 30 1224
Fragments arranged by size:
1224 831 688 638 183 30
PspOMI G'GGCC_C
Cuts at: 0 2575 3594
Size: 2575 1019
PstI C_TGCA'G
Cuts at: 0 1433 1897 2938 3280 3424 3594
Size: 1433 464 1041 342 144 170
Fragments arranged by size:
1433 1041 464 342 170 144
PvuI CG_AT'CG
Cuts at: 0 799 3594
Size: 799 2795
PvuII CAG'CTG
Cuts at: 0 66 964 1766 2919 3305 3594
Size: 66 898 802 1153 386 289
Fragments arranged by size:
1153 898 802 386 289 66
SacI G_AGCT'C
Cuts at: 0 584 3594
Size: 584 3010
SfcI C'TryA_G
Cuts at: 0 666 1429 1893 2534 2934 3276 3420 3522
Size: 666 763 464 641 400 342 144 102
Cuts at: 3522 3594
Size: 72
Fragments arranged by size:
763 666 641 464 400 342 144 102
72
SgrAI Cr'CCGG_yG
Cuts at: 0 1931 3594
Size: 1931 1663
SmlI C'TyrA_G
Cuts at: 0 25 575 833 2955 3594
Size: 25 550 258 2122 639
Fragments arranged by size:
2122 639 550 258 25
SphI G_CATG'C
Cuts at: 0 1201 3594
Size: 1201 2393
StuI AGG'CCT
Cuts at: 0 2200 3594
Size: 2200 1394
StyI C'CwwG_G
Cuts at: 0 1562 1995 2580 2636 2709 3594
Size: 1562 433 585 56 73 885
Fragments arranged by size:
1562 885 585 433 73 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2224 2874 3594
Size: 2224 650 720
Fragments arranged by size:
2224 720 650
TatI w'GTAC_w
Cuts at: 0 551 1230 3065 3594
Size: 551 679 1835 529
Fragments arranged by size:
1835 679 551 529
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1327 1359 2507 2539 3594
Size: 1327 32 1148 32 1055
Fragments arranged by size:
1327 1148 1055 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 719 3594
Size: 719 2875
XhoI C'TCGA_G
Cuts at: 0 833 3594
Size: 833 2761
XmnI GAAnn'nnTTC
Cuts at: 0 2423 3439 3594
Size: 2423 1016 155
Fragments arranged by size:
2423 1016 155
Enzymes that do cut:
AarI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI
BanI BanII BbsI BbvCI BcgI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtsI CspCI DraI
EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI HaeII
Hin4I HincII HindIII KpnI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PpuMI PspOMI PstI PvuI PvuII SacI SfcI SgrAI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XhoI XmnI
Enzymes that do not cut:
AatII AccI AflII AhdI AloI AscI AseI AsiSI
BaeI BbeI BciVI BclI BfrBI BmtI BsaAI BsaHI
BsiWI BsmBI BspEI BspHI BsrBI BssHII BstEII BstZ17I
BtgZI ClaI DraIII DrdI EciI EcoNI EcoRI EcoRV
FseI FspI FspAI HpaI KasI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XmaI ZraI