Restriction map for
pF1KA0374
(Linear) (Six-base) MAPSORT of: KIAA0374.seq Check: 606 from: 1 to: 1614
KIAA0374.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AccI GT'mk_AC
Cuts at: 0 156 1614
Size: 156 1458
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 481 556 1181 1614
Size: 430 51 75 625 433
Fragments arranged by size:
625 433 430 75 51
AhdI GACnn_n'nnGTC
Cuts at: 0 1158 1614
Size: 1158 456
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1082 1614
Size: 1082 532
AlwNI CAG_nnn'CTG
Cuts at: 0 357 1156 1614
Size: 357 799 458
Fragments arranged by size:
799 458 357
ApaI G_GGCC'C
Cuts at: 0 61 985 1205 1614
Size: 61 924 220 409
Fragments arranged by size:
924 409 220 61
AvaI C'yCGr_G
Cuts at: 0 88 1614
Size: 88 1526
BanI G'GyrC_C
Cuts at: 0 220 392 943 1172 1297 1431 1475 1614
Size: 220 172 551 229 125 134 44 139
Fragments arranged by size:
551 229 220 172 139 134 125 44
BanII G_rGCy'C
Cuts at: 0 61 985 1205 1265 1382 1614
Size: 61 924 220 60 117 232
Fragments arranged by size:
924 232 220 117 61 60
BbeI G_GCGC'C
Cuts at: 0 1479 1614
Size: 1479 135
BbsI GAAGACnn'nnnn_
Cuts at: 0 469 1614
Size: 469 1145
BbvCI CC'TCA_GC
Cuts at: 0 81 842 1614
Size: 81 761 772
Fragments arranged by size:
772 761 81
BciVI GTATCCnnnnn_n'
Cuts at: 0 86 1614
Size: 86 1528
BglI GCCn_nnn'nGGC
Cuts at: 0 529 1595 1614
Size: 529 1066 19
Fragments arranged by size:
1066 529 19
BlpI GC'TnA_GC
Cuts at: 0 232 1570 1614
Size: 232 1338 44
Fragments arranged by size:
1338 232 44
Bme1580I G_kGCm'C
Cuts at: 0 61 985 1177 1205 1339 1445 1614
Size: 61 924 192 28 134 106 169
Fragments arranged by size:
924 192 169 134 106 61 28
BmgBI CAC'GTC
Cuts at: 0 32 1614
Size: 32 1582
BmrI ACTGGGnnnn_n'
Cuts at: 0 731 1614
Size: 731 883
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 972 1614
Size: 972 642
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 682 813 1138 1413 1614
Size: 415 267 131 325 275 201
Fragments arranged by size:
415 325 275 267 201 131
Bpu10I CC'TnA_GC
Cuts at: 0 81 842 1614
Size: 81 761 772
Fragments arranged by size:
772 761 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 1614
Size: 550 1064
BsaI GGTCTCn'nnnn_
Cuts at: 0 946 1202 1614
Size: 946 256 412
Fragments arranged by size:
946 412 256
BsaHI Gr'CG_yC
Cuts at: 0 1476 1614
Size: 1476 138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1489 1519 1614
Size: 1489 30 95
Fragments arranged by size:
1489 95 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 69 514 517 964 1363 1366 1369 1614
Size: 69 445 3 447 399 3 3 245
Fragments arranged by size:
447 445 399 245 69 3 3 3
BseYI C'CCAG_C
Cuts at: 0 16 524 1469 1585 1590 1607 1614
Size: 16 508 945 116 5 17 7
Fragments arranged by size:
945 508 116 17 16 7 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 333 658 946 1063 1108 1509 1614
Size: 333 325 288 117 45 401 105
Fragments arranged by size:
401 333 325 288 117 105 45
BsiEI CG_ry'CG
Cuts at: 0 1439 1614
Size: 1439 175
BsmBI CGTCTCn'nnnn_
Cuts at: 0 658 1614
Size: 658 956
Bsp1286I G_dGCh'C
Cuts at: 0 61 985 1177 1205 1265 1339 1382 1445
Size: 61 924 192 28 60 74 43 63
Cuts at: 1445 1614
Size: 169
Fragments arranged by size:
924 192 169 74 63 61 60 43
28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 561 1614
Size: 561 1053
BsrBI CCG'CTC
Cuts at: 0 752 1466 1565 1614
Size: 752 714 99 49
Fragments arranged by size:
752 714 99 49
BsrFI r'CCGG_y
Cuts at: 0 425 733 1434 1614
Size: 425 308 701 180
Fragments arranged by size:
701 425 308 180
BstEII G'GTnAC_C
Cuts at: 0 805 1614
Size: 805 809
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1144 1614
Size: 1144 470
BstYI r'GATC_y
Cuts at: 0 1413 1614
Size: 1413 201
BtgI C'CryG_G
Cuts at: 0 324 1272 1340 1364 1445 1614
Size: 324 948 68 24 81 169
Fragments arranged by size:
948 324 169 81 68 24
BtsI GCAGTG_nn'
Cuts at: 0 273 321 1229 1614
Size: 273 48 908 385
Fragments arranged by size:
908 385 273 48
DraIII CAC_nnn'GTG
Cuts at: 0 311 516 1614
Size: 311 205 1098
Fragments arranged by size:
1098 311 205
DrdI GACnn_nn'nnGTC
Cuts at: 0 471 796 1614
Size: 471 325 818
Fragments arranged by size:
818 471 325
EaeI y'GGCC_r
Cuts at: 0 134 704 1314 1436 1614
Size: 134 570 610 122 178
Fragments arranged by size:
610 570 178 134 122
EagI C'GGCC_G
Cuts at: 0 1436 1614
Size: 1436 178
EarI CTCTTCn'nnn_
Cuts at: 0 231 1340 1614
Size: 231 1109 274
Fragments arranged by size:
1109 274 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 430 481 556 682 813 1138 1181
Size: 415 15 51 75 126 131 325 43
Cuts at: 1181 1413 1614
Size: 232 201
Fragments arranged by size:
415 325 232 201 131 126 75 51
43 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 538 1160 1614
Size: 538 622 454
Fragments arranged by size:
622 538 454
EcoO109I rG'GnC_Cy
Cuts at: 0 58 740 982 1201 1254 1614
Size: 58 682 242 219 53 360
Fragments arranged by size:
682 360 242 219 58 53
HaeII r_GCGC'y
Cuts at: 0 294 1479 1614
Size: 294 1185 135
Fragments arranged by size:
1185 294 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1144 1176 1201 1233 1614
Size: 1144 32 25 32 381
Fragments arranged by size:
1144 381 32 32 25
KasI G'GCGC_C
Cuts at: 0 1475 1614
Size: 1475 139
MscI TGG'CCA
Cuts at: 0 136 706 1316 1614
Size: 136 570 610 298
Fragments arranged by size:
610 570 298 136
MslI CAynn'nnrTG
Cuts at: 0 136 685 1614
Size: 136 549 929
Fragments arranged by size:
929 549 136
MspA1I CmG'CkG
Cuts at: 0 11 471 528 833 1052 1290 1473 1559
Size: 11 460 57 305 219 238 183 86
Cuts at: 1559 1594 1614
Size: 35 20
Fragments arranged by size:
460 305 238 219 183 86 57 35
20 11
NaeI GCC'GGC
Cuts at: 0 1436 1614
Size: 1436 178
NarI GG'CG_CC
Cuts at: 0 1476 1614
Size: 1476 138
NcoI C'CATG_G
Cuts at: 0 324 1340 1614
Size: 324 1016 274
Fragments arranged by size:
1016 324 274
NgoMIV G'CCGG_C
Cuts at: 0 1434 1614
Size: 1434 180
NspI r_CATG'y
Cuts at: 0 483 1059 1614
Size: 483 576 555
Fragments arranged by size:
576 555 483
PflMI CCAn_nnn'nTGG
Cuts at: 0 516 1614
Size: 516 1098
PfoI T'CCnGG_A
Cuts at: 0 431 1115 1614
Size: 431 684 499
Fragments arranged by size:
684 499 431
PpuMI rG'GwC_Cy
Cuts at: 0 740 1614
Size: 740 874
PshAI GACnn'nnGTC
Cuts at: 0 275 1614
Size: 275 1339
PspOMI G'GGCC_C
Cuts at: 0 57 981 1201 1614
Size: 57 924 220 413
Fragments arranged by size:
924 413 220 57
PstI C_TGCA'G
Cuts at: 0 371 623 1614
Size: 371 252 991
Fragments arranged by size:
991 371 252
PvuII CAG'CTG
Cuts at: 0 471 528 1052 1559 1614
Size: 471 57 524 507 55
Fragments arranged by size:
524 507 471 57 55
SanDI GG'GwC_CC
Cuts at: 0 740 1614
Size: 740 874
ScaI AGT'ACT
Cuts at: 0 628 1614
Size: 628 986
SfcI C'TryA_G
Cuts at: 0 367 619 1614
Size: 367 252 995
Fragments arranged by size:
995 367 252
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 529 1614
Size: 529 1085
SfoI GGC'GCC
Cuts at: 0 1477 1614
Size: 1477 137
SmaI CCC'GGG
Cuts at: 0 90 1614
Size: 90 1524
SmlI C'TyrA_G
Cuts at: 0 565 1614
Size: 565 1049
SphI G_CATG'C
Cuts at: 0 483 1059 1614
Size: 483 576 555
Fragments arranged by size:
576 555 483
StyI C'CwwG_G
Cuts at: 0 286 324 707 1340 1614
Size: 286 38 383 633 274
Fragments arranged by size:
633 383 286 274 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1228 1614
Size: 1228 386
TatI w'GTAC_w
Cuts at: 0 626 1614
Size: 626 988
Tth111I GACn'n_nGTC
Cuts at: 0 583 1614
Size: 583 1031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1221 1614
Size: 1221 393
XmaI C'CCGG_G
Cuts at: 0 88 1614
Size: 88 1526
XmnI GAAnn'nnTTC
Cuts at: 0 628 1614
Size: 628 986
Enzymes that do cut:
AccI AcuI AhdI AlfI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BbvCI BciVI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspMI
BsrBI BsrFI BstEII BstXI BstYI BtgI BtsI DraIII
DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I HaeII
Hin4I KasI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PflMI PfoI PpuMI PshAI PspOMI PstI
PvuII SanDI ScaI SfcI SfiI SfoI SmaI SmlI
SphI StyI TaqII TatI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BclI BfrBI BglII BmtI BsaAI
BsaBI BsaWI BsiHKAI BsiWI BsmI BspEI BspHI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI NdeI NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI SexAI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
TstI XbaI XhoI ZraI