(Linear) (Six-base) MAPSORT of: KIAA0379.seq Check: 5979 from: 1 to: 3159 KIAA0379.orf With 182 enzymes: SgfI * October 24, 2006 15:12 .. AccI GT'mk_AC Cuts at: 0 1224 3159 Size: 1224 1935 Acc65I G'GTAC_C Cuts at: 0 378 3159 Size: 378 2781 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 389 611 1163 1546 3159 Size: 389 222 552 383 1613 Fragments arranged by size: 1613 552 389 383 222 AflII C'TTAA_G Cuts at: 0 1140 3159 Size: 1140 2019 AflIII A'CryG_T Cuts at: 0 2308 3159 Size: 2308 851 AleI CACnn'nnGTG Cuts at: 0 2195 3121 3159 Size: 2195 926 38 Fragments arranged by size: 2195 926 38 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 851 1349 1438 2813 3159 Size: 851 498 89 1375 346 Fragments arranged by size: 1375 851 498 346 89 AlwNI CAG_nnn'CTG Cuts at: 0 1359 2523 3159 Size: 1359 1164 636 Fragments arranged by size: 1359 1164 636 ApoI r'AATT_y Cuts at: 0 1248 1306 3159 Size: 1248 58 1853 Fragments arranged by size: 1853 1248 58 AseI AT'TA_AT Cuts at: 0 497 1808 1906 3159 Size: 497 1311 98 1253 Fragments arranged by size: 1311 1253 497 98 AvaI C'yCGr_G Cuts at: 0 305 3159 Size: 305 2854 BamHI G'GATC_C Cuts at: 0 1496 3159 Size: 1496 1663 BanI G'GyrC_C Cuts at: 0 378 487 2434 3159 Size: 378 109 1947 725 Fragments arranged by size: 1947 725 378 109 BanII G_rGCy'C Cuts at: 0 2211 3134 3159 Size: 2211 923 25 Fragments arranged by size: 2211 923 25 BbsI GAAGACnn'nnnn_ Cuts at: 0 1552 3159 Size: 1552 1607 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2389 2423 3159 Size: 2389 34 736 Fragments arranged by size: 2389 736 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1507 3159 Size: 1507 1652 BclI T'GATC_A Cuts at: 0 653 1061 3159 Size: 653 408 2098 Fragments arranged by size: 2098 653 408 BfrBI ATG'CAT Cuts at: 0 2110 3159 Size: 2110 1049 BglII A'GATC_T Cuts at: 0 678 1315 1722 1761 3159 Size: 678 637 407 39 1398 Fragments arranged by size: 1398 678 637 407 39 BlpI GC'TnA_GC Cuts at: 0 2527 3159 Size: 2527 632 Bme1580I G_kGCm'C Cuts at: 0 1994 3159 Size: 1994 1165 BmgBI CAC'GTC Cuts at: 0 3123 3159 Size: 3123 36 BmrI ACTGGGnnnn_n' Cuts at: 0 2292 3159 Size: 2292 867 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 209 1262 3159 Size: 209 1053 1897 Fragments arranged by size: 1897 1053 209 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2525 3159 Size: 2525 634 BsaI GGTCTCn'nnnn_ Cuts at: 0 3145 3159 Size: 3145 14 BsaBI GATnn'nnATC Cuts at: 0 1487 2240 3159 Size: 1487 753 919 Fragments arranged by size: 1487 919 753 BsaHI Gr'CG_yC Cuts at: 0 1963 2161 3159 Size: 1963 198 998 Fragments arranged by size: 1963 998 198 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 182 212 578 608 1373 1403 2018 2048 Size: 182 30 366 30 765 30 615 30 Cuts at: 2048 2837 2867 3159 Size: 789 30 292 Fragments arranged by size: 789 765 615 366 292 182 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1574 2465 3159 Size: 1574 891 694 Fragments arranged by size: 1574 891 694 BseYI C'CCAG_C Cuts at: 0 2883 3159 Size: 2883 276 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1304 1309 1398 1408 2660 3159 Size: 1304 5 89 10 1252 499 Fragments arranged by size: 1304 1252 499 89 10 5 BsiHKAI G_wGCw'C Cuts at: 0 78 2901 3134 3159 Size: 78 2823 233 25 Fragments arranged by size: 2823 233 78 25 BsmI GAATG_Cn' Cuts at: 0 285 1897 1935 3159 Size: 285 1612 38 1224 Fragments arranged by size: 1612 1224 285 38 Bsp1286I G_dGCh'C Cuts at: 0 78 1994 2211 2901 3134 3159 Size: 78 1916 217 690 233 25 Fragments arranged by size: 1916 690 233 217 78 25 BspHI T'CATG_A Cuts at: 0 2703 3050 3159 Size: 2703 347 109 Fragments arranged by size: 2703 347 109 BsrDI GCAATG_nn' Cuts at: 0 130 888 2011 2371 3159 Size: 130 758 1123 360 788 Fragments arranged by size: 1123 788 758 360 130 BssSI C'ACGA_G Cuts at: 0 2302 3159 Size: 2302 857 BstEII G'GTnAC_C Cuts at: 0 1546 1681 3159 Size: 1546 135 1478 Fragments arranged by size: 1546 1478 135 BstYI r'GATC_y Cuts at: 0 57 678 1315 1496 1722 1761 3159 Size: 57 621 637 181 226 39 1398 Fragments arranged by size: 1398 637 621 226 181 57 39 Bsu36I CC'TnA_GG Cuts at: 0 1608 3159 Size: 1608 1551 BtgI C'CryG_G Cuts at: 0 945 3159 Size: 945 2214 BtsI GCAGTG_nn' Cuts at: 0 1091 1321 1940 2637 3159 Size: 1091 230 619 697 522 Fragments arranged by size: 1091 697 619 522 230 EaeI y'GGCC_r Cuts at: 0 1047 2939 3159 Size: 1047 1892 220 Fragments arranged by size: 1892 1047 220 EarI CTCTTCn'nnn_ Cuts at: 0 389 632 731 898 1843 3159 Size: 389 243 99 167 945 1316 Fragments arranged by size: 1316 945 389 243 167 99 EcoICRI GAG'CTC Cuts at: 0 3132 3159 Size: 3132 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 209 389 611 1163 1262 1546 3159 Size: 209 180 222 552 99 284 1613 Fragments arranged by size: 1613 552 284 222 209 180 99 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2987 3159 Size: 2987 172 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 13 45 598 630 977 1009 1053 1085 Size: 13 32 553 32 347 32 44 32 Cuts at: 1085 1808 1840 3159 Size: 723 32 1319 Fragments arranged by size: 1319 723 553 347 44 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 0 102 224 3159 Size: 102 122 2935 Fragments arranged by size: 2935 122 102 HindIII A'AGCT_T Cuts at: 0 371 3038 3159 Size: 371 2667 121 Fragments arranged by size: 2667 371 121 HpaI GTT'AAC Cuts at: 0 102 3159 Size: 102 3057 KpnI G_GTAC'C Cuts at: 0 382 3159 Size: 382 2777 MscI TGG'CCA Cuts at: 0 1049 2941 3159 Size: 1049 1892 218 Fragments arranged by size: 1892 1049 218 MslI CAynn'nnrTG Cuts at: 0 2195 2947 3121 3159 Size: 2195 752 174 38 Fragments arranged by size: 2195 752 174 38 MspA1I CmG'CkG Cuts at: 0 1234 1240 1537 1566 2520 3159 Size: 1234 6 297 29 954 639 Fragments arranged by size: 1234 954 639 297 29 6 NsiI A_TGCA'T Cuts at: 0 2112 3159 Size: 2112 1047 NspI r_CATG'y Cuts at: 0 602 1231 1623 2312 2794 3159 Size: 602 629 392 689 482 365 Fragments arranged by size: 689 629 602 482 392 365 PciI A'CATG_T Cuts at: 0 2308 3159 Size: 2308 851 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2636 2668 3159 Size: 2636 32 491 Fragments arranged by size: 2636 491 32 PstI C_TGCA'G Cuts at: 0 294 1165 1239 2592 3159 Size: 294 871 74 1353 567 Fragments arranged by size: 1353 871 567 294 74 PvuII CAG'CTG Cuts at: 0 1234 1240 1537 1566 2520 3159 Size: 1234 6 297 29 954 639 Fragments arranged by size: 1234 954 639 297 29 6 SacI G_AGCT'C Cuts at: 0 3134 3159 Size: 3134 25 ScaI AGT'ACT Cuts at: 0 3109 3159 Size: 3109 50 SfcI C'TryA_G Cuts at: 0 290 1161 1235 2561 2588 3159 Size: 290 871 74 1326 27 571 Fragments arranged by size: 1326 871 571 290 74 27 SmlI C'TyrA_G Cuts at: 0 305 1140 2540 3159 Size: 305 835 1400 619 Fragments arranged by size: 1400 835 619 305 SpeI A'CTAG_T Cuts at: 0 2710 3159 Size: 2710 449 SspI AAT'ATT Cuts at: 0 83 495 3159 Size: 83 412 2664 Fragments arranged by size: 2664 412 83 StyI C'CwwG_G Cuts at: 0 150 3159 Size: 150 3009 TatI w'GTAC_w Cuts at: 0 1800 3107 3159 Size: 1800 1307 52 Fragments arranged by size: 1800 1307 52 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 578 610 1373 1405 2837 2869 3159 Size: 578 32 763 32 1432 32 290 Fragments arranged by size: 1432 763 578 290 32 32 32 XbaI T'CTAG_A Cuts at: 0 481 675 1587 3159 Size: 481 194 912 1572 Fragments arranged by size: 1572 912 481 194 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2910 3159 Size: 2910 249 XhoI C'TCGA_G Cuts at: 0 305 3159 Size: 305 2854 XmnI GAAnn'nnTTC Cuts at: 0 266 3159 Size: 266 2893 Enzymes that do cut: AccI Acc65I AcuI AflII AflIII AleI AlfI AlwNI ApoI AseI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BssSI BstEII BstYI Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI FalI Hin4I HincII HindIII HpaI KpnI MscI MslI MspA1I NsiI NspI PciI PsrI PstI PvuII SacI ScaI SfcI SmlI SpeI SspI StyI TatI TstI XbaI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AgeI AhdI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BbeI BbvCI BglI BmtI BplI Bpu10I BsaAI BsaWI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII KasI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI StuI SwaI TaqII Tth111I XmaI ZraI