Restriction map for pF1KSDA0379
 (Linear) (Six-base) MAPSORT of: KIAA0379.seq  Check: 5979  from: 1  to: 3159



KIAA0379.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AccI GT'mk_AC

Cuts at:      0    1224    3159 
   Size:       1224    1935
 
Acc65I G'GTAC_C

Cuts at:      0     378    3159 
   Size:        378    2781
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     389     611    1163    1546    3159 
   Size:        389     222     552     383    1613

  Fragments arranged by size: 

                1613     552     389     383     222
 
AflII C'TTAA_G

Cuts at:      0    1140    3159 
   Size:       1140    2019
 
AflIII A'CryG_T

Cuts at:      0    2308    3159 
   Size:       2308     851
 
AleI CACnn'nnGTG

Cuts at:      0    2195    3121    3159 
   Size:       2195     926      38

  Fragments arranged by size: 

                2195     926      38
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     851    1349    1438    2813    3159 
   Size:        851     498      89    1375     346

  Fragments arranged by size: 

                1375     851     498     346      89
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1359    2523    3159 
   Size:       1359    1164     636

  Fragments arranged by size: 

                1359    1164     636
 
ApoI r'AATT_y

Cuts at:      0    1248    1306    3159 
   Size:       1248      58    1853

  Fragments arranged by size: 

                1853    1248      58
 
AseI AT'TA_AT

Cuts at:      0     497    1808    1906    3159 
   Size:        497    1311      98    1253

  Fragments arranged by size: 

                1311    1253     497      98
 
AvaI C'yCGr_G

Cuts at:      0     305    3159 
   Size:        305    2854
 
BamHI G'GATC_C

Cuts at:      0    1496    3159 
   Size:       1496    1663
 
BanI G'GyrC_C

Cuts at:      0     378     487    2434    3159 
   Size:        378     109    1947     725

  Fragments arranged by size: 

                1947     725     378     109
 
BanII G_rGCy'C

Cuts at:      0    2211    3134    3159 
   Size:       2211     923      25

  Fragments arranged by size: 

                2211     923      25
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1552    3159 
   Size:       1552    1607
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2389    2423    3159 
   Size:       2389      34     736

  Fragments arranged by size: 

                2389     736      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1507    3159 
   Size:       1507    1652
 
BclI T'GATC_A

Cuts at:      0     653    1061    3159 
   Size:        653     408    2098

  Fragments arranged by size: 

                2098     653     408
 
BfrBI ATG'CAT

Cuts at:      0    2110    3159 
   Size:       2110    1049
 
BglII A'GATC_T

Cuts at:      0     678    1315    1722    1761    3159 
   Size:        678     637     407      39    1398

  Fragments arranged by size: 

                1398     678     637     407      39
 
BlpI GC'TnA_GC

Cuts at:      0    2527    3159 
   Size:       2527     632
 
Bme1580I G_kGCm'C

Cuts at:      0    1994    3159 
   Size:       1994    1165
 
BmgBI CAC'GTC

Cuts at:      0    3123    3159 
   Size:       3123      36
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2292    3159 
   Size:       2292     867
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209    1262    3159 
   Size:        209    1053    1897

  Fragments arranged by size: 

                1897    1053     209
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2525    3159 
   Size:       2525     634
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3145    3159 
   Size:       3145      14
 
BsaBI GATnn'nnATC

Cuts at:      0    1487    2240    3159 
   Size:       1487     753     919

  Fragments arranged by size: 

                1487     919     753
 
BsaHI Gr'CG_yC

Cuts at:      0    1963    2161    3159 
   Size:       1963     198     998

  Fragments arranged by size: 

                1963     998     198
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     182     212     578     608    1373    1403    2018    2048 
   Size:        182      30     366      30     765      30     615      30

Cuts at:   2048    2837    2867    3159 
   Size:        789      30     292

  Fragments arranged by size: 

                 789     765     615     366     292     182      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1574    2465    3159 
   Size:       1574     891     694

  Fragments arranged by size: 

                1574     891     694
 
BseYI C'CCAG_C

Cuts at:      0    2883    3159 
   Size:       2883     276
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1304    1309    1398    1408    2660    3159 
   Size:       1304       5      89      10    1252     499

  Fragments arranged by size: 

                1304    1252     499      89      10       5
 
BsiHKAI G_wGCw'C

Cuts at:      0      78    2901    3134    3159 
   Size:         78    2823     233      25

  Fragments arranged by size: 

                2823     233      78      25
 
BsmI GAATG_Cn'

Cuts at:      0     285    1897    1935    3159 
   Size:        285    1612      38    1224

  Fragments arranged by size: 

                1612    1224     285      38
 
Bsp1286I G_dGCh'C

Cuts at:      0      78    1994    2211    2901    3134    3159 
   Size:         78    1916     217     690     233      25

  Fragments arranged by size: 

                1916     690     233     217      78      25
 
BspHI T'CATG_A

Cuts at:      0    2703    3050    3159 
   Size:       2703     347     109

  Fragments arranged by size: 

                2703     347     109
 
BsrDI GCAATG_nn'

Cuts at:      0     130     888    2011    2371    3159 
   Size:        130     758    1123     360     788

  Fragments arranged by size: 

                1123     788     758     360     130
 
BssSI C'ACGA_G

Cuts at:      0    2302    3159 
   Size:       2302     857
 
BstEII G'GTnAC_C

Cuts at:      0    1546    1681    3159 
   Size:       1546     135    1478

  Fragments arranged by size: 

                1546    1478     135
 
BstYI r'GATC_y

Cuts at:      0      57     678    1315    1496    1722    1761    3159 
   Size:         57     621     637     181     226      39    1398

  Fragments arranged by size: 

                1398     637     621     226     181      57      39
 
Bsu36I CC'TnA_GG

Cuts at:      0    1608    3159 
   Size:       1608    1551
 
BtgI C'CryG_G

Cuts at:      0     945    3159 
   Size:        945    2214
 
BtsI GCAGTG_nn'

Cuts at:      0    1091    1321    1940    2637    3159 
   Size:       1091     230     619     697     522

  Fragments arranged by size: 

                1091     697     619     522     230
 
EaeI y'GGCC_r

Cuts at:      0    1047    2939    3159 
   Size:       1047    1892     220

  Fragments arranged by size: 

                1892    1047     220
 
EarI CTCTTCn'nnn_

Cuts at:      0     389     632     731     898    1843    3159 
   Size:        389     243      99     167     945    1316

  Fragments arranged by size: 

                1316     945     389     243     167      99
 
EcoICRI GAG'CTC

Cuts at:      0    3132    3159 
   Size:       3132      27
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     389     611    1163    1262    1546    3159 
   Size:        209     180     222     552      99     284    1613

  Fragments arranged by size: 

                1613     552     284     222     209     180      99
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2987    3159 
   Size:       2987     172
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      13      45     598     630     977    1009    1053    1085 
   Size:         13      32     553      32     347      32      44      32

Cuts at:   1085    1808    1840    3159 
   Size:        723      32    1319

  Fragments arranged by size: 

                1319     723     553     347      44      32      32      32
                  32      32      13
 
HincII GTy'rAC

Cuts at:      0     102     224    3159 
   Size:        102     122    2935

  Fragments arranged by size: 

                2935     122     102
 
HindIII A'AGCT_T

Cuts at:      0     371    3038    3159 
   Size:        371    2667     121

  Fragments arranged by size: 

                2667     371     121
 
HpaI GTT'AAC

Cuts at:      0     102    3159 
   Size:        102    3057
 
KpnI G_GTAC'C

Cuts at:      0     382    3159 
   Size:        382    2777
 
MscI TGG'CCA

Cuts at:      0    1049    2941    3159 
   Size:       1049    1892     218

  Fragments arranged by size: 

                1892    1049     218
 
MslI CAynn'nnrTG

Cuts at:      0    2195    2947    3121    3159 
   Size:       2195     752     174      38

  Fragments arranged by size: 

                2195     752     174      38
 
MspA1I CmG'CkG

Cuts at:      0    1234    1240    1537    1566    2520    3159 
   Size:       1234       6     297      29     954     639

  Fragments arranged by size: 

                1234     954     639     297      29       6
 
NsiI A_TGCA'T

Cuts at:      0    2112    3159 
   Size:       2112    1047
 
NspI r_CATG'y

Cuts at:      0     602    1231    1623    2312    2794    3159 
   Size:        602     629     392     689     482     365

  Fragments arranged by size: 

                 689     629     602     482     392     365
 
PciI A'CATG_T

Cuts at:      0    2308    3159 
   Size:       2308     851
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2636    2668    3159 
   Size:       2636      32     491

  Fragments arranged by size: 

                2636     491      32
 
PstI C_TGCA'G

Cuts at:      0     294    1165    1239    2592    3159 
   Size:        294     871      74    1353     567

  Fragments arranged by size: 

                1353     871     567     294      74
 
PvuII CAG'CTG

Cuts at:      0    1234    1240    1537    1566    2520    3159 
   Size:       1234       6     297      29     954     639

  Fragments arranged by size: 

                1234     954     639     297      29       6
 
SacI G_AGCT'C

Cuts at:      0    3134    3159 
   Size:       3134      25
 
ScaI AGT'ACT

Cuts at:      0    3109    3159 
   Size:       3109      50
 
SfcI C'TryA_G

Cuts at:      0     290    1161    1235    2561    2588    3159 
   Size:        290     871      74    1326      27     571

  Fragments arranged by size: 

                1326     871     571     290      74      27
 
SmlI C'TyrA_G

Cuts at:      0     305    1140    2540    3159 
   Size:        305     835    1400     619

  Fragments arranged by size: 

                1400     835     619     305
 
SpeI A'CTAG_T

Cuts at:      0    2710    3159 
   Size:       2710     449
 
SspI AAT'ATT

Cuts at:      0      83     495    3159 
   Size:         83     412    2664

  Fragments arranged by size: 

                2664     412      83
 
StyI C'CwwG_G

Cuts at:      0     150    3159 
   Size:        150    3009
 
TatI w'GTAC_w

Cuts at:      0    1800    3107    3159 
   Size:       1800    1307      52

  Fragments arranged by size: 

                1800    1307      52
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     578     610    1373    1405    2837    2869    3159 
   Size:        578      32     763      32    1432      32     290

  Fragments arranged by size: 

                1432     763     578     290      32      32      32
 
XbaI T'CTAG_A

Cuts at:      0     481     675    1587    3159 
   Size:        481     194     912    1572

  Fragments arranged by size: 

                1572     912     481     194
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2910    3159 
   Size:       2910     249
 
XhoI C'TCGA_G

Cuts at:      0     305    3159 
   Size:        305    2854
 
XmnI GAAnn'nnTTC

Cuts at:      0     266    3159 
   Size:        266    2893
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AflII   AflIII     AleI     AlfI    AlwNI
     ApoI     AseI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BpmI    BpuEI     BsaI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI    BssSI   BstEII
    BstYI   Bsu36I     BtgI     BtsI     EaeI     EarI  EcoICRI  Eco57MI
     FalI    Hin4I   HincII  HindIII     HpaI     KpnI     MscI     MslI
   MspA1I     NsiI     NspI     PciI     PsrI     PstI    PvuII     SacI
     ScaI     SfcI     SmlI     SpeI     SspI     StyI     TatI     TstI
     XbaI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI     AgeI     AhdI     AloI     ApaI
    ApaLI     AscI    AsiSI    AvrII     BaeI     BbeI    BbvCI     BglI
     BmtI     BplI   Bpu10I    BsaAI    BsaWI    BsiEI    BsiWI    BsmBI
    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII     KasI     MfeI     MluI     MmeI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SphI     SrfI     StuI     SwaI    TaqII  Tth111I     XmaI     ZraI
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