Restriction map for
pF1KSDA0379
(Linear) (Six-base) MAPSORT of: KIAA0379.seq Check: 5979 from: 1 to: 3159
KIAA0379.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AccI GT'mk_AC
Cuts at: 0 1224 3159
Size: 1224 1935
Acc65I G'GTAC_C
Cuts at: 0 378 3159
Size: 378 2781
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 611 1163 1546 3159
Size: 389 222 552 383 1613
Fragments arranged by size:
1613 552 389 383 222
AflII C'TTAA_G
Cuts at: 0 1140 3159
Size: 1140 2019
AflIII A'CryG_T
Cuts at: 0 2308 3159
Size: 2308 851
AleI CACnn'nnGTG
Cuts at: 0 2195 3121 3159
Size: 2195 926 38
Fragments arranged by size:
2195 926 38
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 851 1349 1438 2813 3159
Size: 851 498 89 1375 346
Fragments arranged by size:
1375 851 498 346 89
AlwNI CAG_nnn'CTG
Cuts at: 0 1359 2523 3159
Size: 1359 1164 636
Fragments arranged by size:
1359 1164 636
ApoI r'AATT_y
Cuts at: 0 1248 1306 3159
Size: 1248 58 1853
Fragments arranged by size:
1853 1248 58
AseI AT'TA_AT
Cuts at: 0 497 1808 1906 3159
Size: 497 1311 98 1253
Fragments arranged by size:
1311 1253 497 98
AvaI C'yCGr_G
Cuts at: 0 305 3159
Size: 305 2854
BamHI G'GATC_C
Cuts at: 0 1496 3159
Size: 1496 1663
BanI G'GyrC_C
Cuts at: 0 378 487 2434 3159
Size: 378 109 1947 725
Fragments arranged by size:
1947 725 378 109
BanII G_rGCy'C
Cuts at: 0 2211 3134 3159
Size: 2211 923 25
Fragments arranged by size:
2211 923 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 1552 3159
Size: 1552 1607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2389 2423 3159
Size: 2389 34 736
Fragments arranged by size:
2389 736 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1507 3159
Size: 1507 1652
BclI T'GATC_A
Cuts at: 0 653 1061 3159
Size: 653 408 2098
Fragments arranged by size:
2098 653 408
BfrBI ATG'CAT
Cuts at: 0 2110 3159
Size: 2110 1049
BglII A'GATC_T
Cuts at: 0 678 1315 1722 1761 3159
Size: 678 637 407 39 1398
Fragments arranged by size:
1398 678 637 407 39
BlpI GC'TnA_GC
Cuts at: 0 2527 3159
Size: 2527 632
Bme1580I G_kGCm'C
Cuts at: 0 1994 3159
Size: 1994 1165
BmgBI CAC'GTC
Cuts at: 0 3123 3159
Size: 3123 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 2292 3159
Size: 2292 867
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 1262 3159
Size: 209 1053 1897
Fragments arranged by size:
1897 1053 209
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2525 3159
Size: 2525 634
BsaI GGTCTCn'nnnn_
Cuts at: 0 3145 3159
Size: 3145 14
BsaBI GATnn'nnATC
Cuts at: 0 1487 2240 3159
Size: 1487 753 919
Fragments arranged by size:
1487 919 753
BsaHI Gr'CG_yC
Cuts at: 0 1963 2161 3159
Size: 1963 198 998
Fragments arranged by size:
1963 998 198
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 182 212 578 608 1373 1403 2018 2048
Size: 182 30 366 30 765 30 615 30
Cuts at: 2048 2837 2867 3159
Size: 789 30 292
Fragments arranged by size:
789 765 615 366 292 182 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1574 2465 3159
Size: 1574 891 694
Fragments arranged by size:
1574 891 694
BseYI C'CCAG_C
Cuts at: 0 2883 3159
Size: 2883 276
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1304 1309 1398 1408 2660 3159
Size: 1304 5 89 10 1252 499
Fragments arranged by size:
1304 1252 499 89 10 5
BsiHKAI G_wGCw'C
Cuts at: 0 78 2901 3134 3159
Size: 78 2823 233 25
Fragments arranged by size:
2823 233 78 25
BsmI GAATG_Cn'
Cuts at: 0 285 1897 1935 3159
Size: 285 1612 38 1224
Fragments arranged by size:
1612 1224 285 38
Bsp1286I G_dGCh'C
Cuts at: 0 78 1994 2211 2901 3134 3159
Size: 78 1916 217 690 233 25
Fragments arranged by size:
1916 690 233 217 78 25
BspHI T'CATG_A
Cuts at: 0 2703 3050 3159
Size: 2703 347 109
Fragments arranged by size:
2703 347 109
BsrDI GCAATG_nn'
Cuts at: 0 130 888 2011 2371 3159
Size: 130 758 1123 360 788
Fragments arranged by size:
1123 788 758 360 130
BssSI C'ACGA_G
Cuts at: 0 2302 3159
Size: 2302 857
BstEII G'GTnAC_C
Cuts at: 0 1546 1681 3159
Size: 1546 135 1478
Fragments arranged by size:
1546 1478 135
BstYI r'GATC_y
Cuts at: 0 57 678 1315 1496 1722 1761 3159
Size: 57 621 637 181 226 39 1398
Fragments arranged by size:
1398 637 621 226 181 57 39
Bsu36I CC'TnA_GG
Cuts at: 0 1608 3159
Size: 1608 1551
BtgI C'CryG_G
Cuts at: 0 945 3159
Size: 945 2214
BtsI GCAGTG_nn'
Cuts at: 0 1091 1321 1940 2637 3159
Size: 1091 230 619 697 522
Fragments arranged by size:
1091 697 619 522 230
EaeI y'GGCC_r
Cuts at: 0 1047 2939 3159
Size: 1047 1892 220
Fragments arranged by size:
1892 1047 220
EarI CTCTTCn'nnn_
Cuts at: 0 389 632 731 898 1843 3159
Size: 389 243 99 167 945 1316
Fragments arranged by size:
1316 945 389 243 167 99
EcoICRI GAG'CTC
Cuts at: 0 3132 3159
Size: 3132 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 389 611 1163 1262 1546 3159
Size: 209 180 222 552 99 284 1613
Fragments arranged by size:
1613 552 284 222 209 180 99
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2987 3159
Size: 2987 172
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 598 630 977 1009 1053 1085
Size: 13 32 553 32 347 32 44 32
Cuts at: 1085 1808 1840 3159
Size: 723 32 1319
Fragments arranged by size:
1319 723 553 347 44 32 32 32
32 32 13
HincII GTy'rAC
Cuts at: 0 102 224 3159
Size: 102 122 2935
Fragments arranged by size:
2935 122 102
HindIII A'AGCT_T
Cuts at: 0 371 3038 3159
Size: 371 2667 121
Fragments arranged by size:
2667 371 121
HpaI GTT'AAC
Cuts at: 0 102 3159
Size: 102 3057
KpnI G_GTAC'C
Cuts at: 0 382 3159
Size: 382 2777
MscI TGG'CCA
Cuts at: 0 1049 2941 3159
Size: 1049 1892 218
Fragments arranged by size:
1892 1049 218
MslI CAynn'nnrTG
Cuts at: 0 2195 2947 3121 3159
Size: 2195 752 174 38
Fragments arranged by size:
2195 752 174 38
MspA1I CmG'CkG
Cuts at: 0 1234 1240 1537 1566 2520 3159
Size: 1234 6 297 29 954 639
Fragments arranged by size:
1234 954 639 297 29 6
NsiI A_TGCA'T
Cuts at: 0 2112 3159
Size: 2112 1047
NspI r_CATG'y
Cuts at: 0 602 1231 1623 2312 2794 3159
Size: 602 629 392 689 482 365
Fragments arranged by size:
689 629 602 482 392 365
PciI A'CATG_T
Cuts at: 0 2308 3159
Size: 2308 851
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2636 2668 3159
Size: 2636 32 491
Fragments arranged by size:
2636 491 32
PstI C_TGCA'G
Cuts at: 0 294 1165 1239 2592 3159
Size: 294 871 74 1353 567
Fragments arranged by size:
1353 871 567 294 74
PvuII CAG'CTG
Cuts at: 0 1234 1240 1537 1566 2520 3159
Size: 1234 6 297 29 954 639
Fragments arranged by size:
1234 954 639 297 29 6
SacI G_AGCT'C
Cuts at: 0 3134 3159
Size: 3134 25
ScaI AGT'ACT
Cuts at: 0 3109 3159
Size: 3109 50
SfcI C'TryA_G
Cuts at: 0 290 1161 1235 2561 2588 3159
Size: 290 871 74 1326 27 571
Fragments arranged by size:
1326 871 571 290 74 27
SmlI C'TyrA_G
Cuts at: 0 305 1140 2540 3159
Size: 305 835 1400 619
Fragments arranged by size:
1400 835 619 305
SpeI A'CTAG_T
Cuts at: 0 2710 3159
Size: 2710 449
SspI AAT'ATT
Cuts at: 0 83 495 3159
Size: 83 412 2664
Fragments arranged by size:
2664 412 83
StyI C'CwwG_G
Cuts at: 0 150 3159
Size: 150 3009
TatI w'GTAC_w
Cuts at: 0 1800 3107 3159
Size: 1800 1307 52
Fragments arranged by size:
1800 1307 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 578 610 1373 1405 2837 2869 3159
Size: 578 32 763 32 1432 32 290
Fragments arranged by size:
1432 763 578 290 32 32 32
XbaI T'CTAG_A
Cuts at: 0 481 675 1587 3159
Size: 481 194 912 1572
Fragments arranged by size:
1572 912 481 194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2910 3159
Size: 2910 249
XhoI C'TCGA_G
Cuts at: 0 305 3159
Size: 305 2854
XmnI GAAnn'nnTTC
Cuts at: 0 266 3159
Size: 266 2893
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AleI AlfI AlwNI
ApoI AseI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BpmI BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BssSI BstEII
BstYI Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI
FalI Hin4I HincII HindIII HpaI KpnI MscI MslI
MspA1I NsiI NspI PciI PsrI PstI PvuII SacI
ScaI SfcI SmlI SpeI SspI StyI TatI TstI
XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AloI ApaI
ApaLI AscI AsiSI AvrII BaeI BbeI BbvCI BglI
BmtI BplI Bpu10I BsaAI BsaWI BsiEI BsiWI BsmBI
BspEI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI
BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII KasI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PvuI RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI StuI SwaI TaqII Tth111I XmaI ZraI