Restriction map for
pF1KSDA0380
(Linear) (Six-base) MAPSORT of: KIAA0380.seq Check: 6574 from: 1 to: 4566
KIAA0380.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2255 4566
Size: 2255 2311
AatII G_ACGT'C
Cuts at: 0 3190 4566
Size: 3190 1376
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 970 2255 3034 3048 3944 4064 4566
Size: 970 1285 779 14 896 120 502
Fragments arranged by size:
1285 970 896 779 502 120 14
AflIII A'CryG_T
Cuts at: 0 3353 4566
Size: 3353 1213
AgeI A'CCGG_T
Cuts at: 0 832 4566
Size: 832 3734
AhdI GACnn_n'nnGTC
Cuts at: 0 122 4566
Size: 122 4444
AleI CACnn'nnGTG
Cuts at: 0 286 436 4387 4566
Size: 286 150 3951 179
Fragments arranged by size:
3951 286 179 150
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1379 1751 2501 4566
Size: 1379 372 750 2065
Fragments arranged by size:
2065 1379 750 372
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3470 3502 4566
Size: 3470 32 1064
Fragments arranged by size:
3470 1064 32
AlwNI CAG_nnn'CTG
Cuts at: 0 739 939 1747 3435 4033 4566
Size: 739 200 808 1688 598 533
Fragments arranged by size:
1688 808 739 598 533 200
ApaI G_GGCC'C
Cuts at: 0 2727 3157 3319 4496 4566
Size: 2727 430 162 1177 70
Fragments arranged by size:
2727 1177 430 162 70
ApoI r'AATT_y
Cuts at: 0 3879 4566
Size: 3879 687
AvaI C'yCGr_G
Cuts at: 0 897 2312 2462 2726 3296 3688 4566
Size: 897 1415 150 264 570 392 878
Fragments arranged by size:
1415 897 878 570 392 264 150
BamHI G'GATC_C
Cuts at: 0 510 1451 4566
Size: 510 941 3115
Fragments arranged by size:
3115 941 510
BanI G'GyrC_C
Cuts at: 0 280 334 2695 3248 4134 4378 4566
Size: 280 54 2361 553 886 244 188
Fragments arranged by size:
2361 886 553 280 244 188 54
BanII G_rGCy'C
Cuts at: 0 364 395 423 811 2727 3043 3157 3218
Size: 364 31 28 388 1916 316 114 61
Cuts at: 3218 3319 3590 3689 4147 4457 4496 4566
Size: 101 271 99 458 310 39 70
Fragments arranged by size:
1916 458 388 364 316 310 271 114
101 99 70 61 39 31 28
BbeI G_GCGC'C
Cuts at: 0 338 4566
Size: 338 4228
BbsI GAAGACnn'nnnn_
Cuts at: 0 38 893 1403 1450 3839 4535 4566
Size: 38 855 510 47 2389 696 31
Fragments arranged by size:
2389 855 696 510 47 38 31
BbvCI CC'TCA_GC
Cuts at: 0 556 3977 4151 4187 4193 4566
Size: 556 3421 174 36 6 373
Fragments arranged by size:
3421 556 373 174 36 6
BclI T'GATC_A
Cuts at: 0 323 770 4566
Size: 323 447 3796
Fragments arranged by size:
3796 447 323
BglI GCCn_nnn'nGGC
Cuts at: 0 238 784 1847 4566
Size: 238 546 1063 2719
Fragments arranged by size:
2719 1063 546 238
BglII A'GATC_T
Cuts at: 0 3164 4566
Size: 3164 1402
Bme1580I G_kGCm'C
Cuts at: 0 1892 2727 3157 3319 4496 4566
Size: 1892 835 430 162 1177 70
Fragments arranged by size:
1892 1177 835 430 162 70
BmgBI CAC'GTC
Cuts at: 0 434 3354 4263 4566
Size: 434 2920 909 303
Fragments arranged by size:
2920 909 434 303
BmrI ACTGGGnnnn_n'
Cuts at: 0 1004 1282 1491 2042 3407 3490 4498 4566
Size: 1004 278 209 551 1365 83 1008 68
Fragments arranged by size:
1365 1008 1004 551 278 209 83 68
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2488 2606 4166 4363 4527 4566
Size: 2488 118 1560 197 164 39
Fragments arranged by size:
2488 1560 197 164 118 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 379 961 1126 1813 1953 2140 2458
Size: 242 137 582 165 687 140 187 318
Cuts at: 2458 2686 2854 2940 2986 3410 3829 3884 4455
Size: 228 168 86 46 424 419 55 571
Cuts at: 4455 4566
Size: 111
Fragments arranged by size:
687 582 571 424 419 318 242 228
187 168 165 140 137 111 86 55
46
Bpu10I CC'TnA_GC
Cuts at: 0 556 3449 3977 4151 4187 4193 4448 4566
Size: 556 2893 528 174 36 6 255 118
Fragments arranged by size:
2893 556 528 255 174 118 36 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1196 1828 2011 2734 3061 4098 4285 4300
Size: 1196 632 183 723 327 1037 187 15
Cuts at: 4300 4333 4566
Size: 33 233
Fragments arranged by size:
1196 1037 723 632 327 233 187 183
33 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 130 609 1874 1991 2609 3847 4566
Size: 130 479 1265 117 618 1238 719
Fragments arranged by size:
1265 1238 719 618 479 130 117
BsaAI yAC'GTr
Cuts at: 0 2955 4566
Size: 2955 1611
BsaHI Gr'CG_yC
Cuts at: 0 335 3187 4224 4517 4566
Size: 335 2852 1037 293 49
Fragments arranged by size:
2852 1037 335 293 49
BsaWI w'CCGG_w
Cuts at: 0 260 832 1768 4566
Size: 260 572 936 2798
Fragments arranged by size:
2798 936 572 260
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 338 368 3736 3766 4566
Size: 338 30 3368 30 800
Fragments arranged by size:
3368 800 338 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 449 2332 3033 3394 4229 4566
Size: 449 1883 701 361 835 337
Fragments arranged by size:
1883 835 701 449 361 337
BseYI C'CCAG_C
Cuts at: 0 411 788 1278 1447 2176 2186 3150 3308
Size: 411 377 490 169 729 10 964 158
Cuts at: 3308 3325 3404 3794 4228 4566
Size: 17 79 390 434 338
Fragments arranged by size:
964 729 490 434 411 390 377 338
169 158 79 17 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 226 248 1055 1927 2046 2471 3123
Size: 47 179 22 807 872 119 425 652
Cuts at: 3123 3485 3989 4566
Size: 362 504 577
Fragments arranged by size:
872 807 652 577 504 425 362 179
119 47 22
BsiEI CG_ry'CG
Cuts at: 0 261 1769 4566
Size: 261 1508 2797
Fragments arranged by size:
2797 1508 261
BsiHKAI G_wGCw'C
Cuts at: 0 423 3077 3095 3218 4566
Size: 423 2654 18 123 1348
Fragments arranged by size:
2654 1348 423 123 18
BsmI GAATG_Cn'
Cuts at: 0 3243 4566
Size: 3243 1323
Bsp1286I G_dGCh'C
Cuts at: 0 364 395 423 811 1892 2727 3043 3077
Size: 364 31 28 388 1081 835 316 34
Cuts at: 3077 3095 3157 3218 3319 3590 3689 4147 4457
Size: 18 62 61 101 271 99 458 310
Cuts at: 4457 4496 4566
Size: 39 70
Fragments arranged by size:
1081 835 458 388 364 316 310 271
101 99 70 62 61 39 34 31
28 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1313 1796 2255 2351 3629 4566
Size: 1313 483 459 96 1278 937
Fragments arranged by size:
1313 1278 937 483 459 96
BsrBI CCG'CTC
Cuts at: 0 1832 1895 3101 3601 4235 4566
Size: 1832 63 1206 500 634 331
Fragments arranged by size:
1832 1206 634 500 331 63
BsrDI GCAATG_nn'
Cuts at: 0 1695 4566
Size: 1695 2871
BsrFI r'CCGG_y
Cuts at: 0 241 629 832 2201 4566
Size: 241 388 203 1369 2365
Fragments arranged by size:
2365 1369 388 241 203
BstAPI GCAn_nnn'nTGC
Cuts at: 0 213 4566
Size: 213 4353
BstEII G'GTnAC_C
Cuts at: 0 11 288 835 4566
Size: 11 277 547 3731
Fragments arranged by size:
3731 547 277 11
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2832 3510 3555 4389 4566
Size: 2832 678 45 834 177
Fragments arranged by size:
2832 834 678 177 45
BstYI r'GATC_y
Cuts at: 0 510 539 936 1133 1232 1244 1451 2877
Size: 510 29 397 197 99 12 207 1426
Cuts at: 2877 3164 4566
Size: 287 1402
Fragments arranged by size:
1426 1402 510 397 287 207 197 99
29 12
Bsu36I CC'TnA_GG
Cuts at: 0 544 1781 3377 4255 4502 4566
Size: 544 1237 1596 878 247 64
Fragments arranged by size:
1596 1237 878 544 247 64
BtgI C'CryG_G
Cuts at: 0 284 377 1191 1412 3360 3662 4566
Size: 284 93 814 221 1948 302 904
Fragments arranged by size:
1948 904 814 302 284 221 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 76 4566
Size: 76 4490
BtsI GCAGTG_nn'
Cuts at: 0 1015 1908 3468 4566
Size: 1015 893 1560 1098
Fragments arranged by size:
1560 1098 1015 893
EaeI y'GGCC_r
Cuts at: 0 776 958 3107 3820 4020 4566
Size: 776 182 2149 713 200 546
Fragments arranged by size:
2149 776 713 546 200 182
EarI CTCTTCn'nnn_
Cuts at: 0 369 1543 1641 1850 2338 2569 3218 3467
Size: 369 1174 98 209 488 231 649 249
Cuts at: 3467 4566
Size: 1099
Fragments arranged by size:
1174 1099 649 488 369 249 231 209
98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1018 4070 4566
Size: 1018 3052 496
Fragments arranged by size:
3052 1018 496
EcoICRI GAG'CTC
Cuts at: 0 421 3216 4566
Size: 421 2795 1350
Fragments arranged by size:
2795 1350 421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 379 961 970 1126 1813 1953 2140
Size: 242 137 582 9 156 687 140 187
Cuts at: 2140 2255 2458 2686 2854 2940 2986 3034 3048
Size: 115 203 228 168 86 46 48 14
Cuts at: 3048 3410 3829 3884 3944 4064 4455 4566
Size: 362 419 55 60 120 391 111
Fragments arranged by size:
687 582 419 391 362 242 228 203
187 168 156 140 137 120 115 111
86 60 55 48 46 14 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2089 3439 4566
Size: 2089 1350 1127
Fragments arranged by size:
2089 1350 1127
EcoO109I rG'GnC_Cy
Cuts at: 0 402 493 1323 1785 2262 2407 2908 2970
Size: 402 91 830 462 477 145 501 62
Cuts at: 2970 3292 3315 3316 3401 3432 3444 3568 3744
Size: 322 23 1 85 31 12 124 176
Cuts at: 3744 4102 4492 4493 4566
Size: 358 390 1 73
Fragments arranged by size:
830 501 477 462 402 390 358 322
176 145 124 91 85 73 62 31
23 12 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1596 3078 4566
Size: 1596 1482 1488
Fragments arranged by size:
1596 1488 1482
FspI TGC'GCA
Cuts at: 0 2251 4566
Size: 2251 2315
HaeII r_GCGC'y
Cuts at: 0 338 1341 1409 4566
Size: 338 1003 68 3157
Fragments arranged by size:
3157 1003 338 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 108 140 1118 1150 1366 1398
Size: 61 32 15 32 978 32 216 32
Cuts at: 1398 2546 2578 2606 2638 2862 2894 3617 3649
Size: 1148 32 28 32 224 32 723 32
Cuts at: 3649 3736 3768 4170 4202 4566
Size: 87 32 402 32 364
Fragments arranged by size:
1148 978 723 402 364 224 216 87
61 32 32 32 32 32 32 32
32 32 32 28 15
HincII GTy'rAC
Cuts at: 0 276 1911 4566
Size: 276 1635 2655
Fragments arranged by size:
2655 1635 276
HindIII A'AGCT_T
Cuts at: 0 1074 1773 2237 4566
Size: 1074 699 464 2329
Fragments arranged by size:
2329 1074 699 464
KasI G'GCGC_C
Cuts at: 0 334 4566
Size: 334 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1502 4566
Size: 1502 3064
MscI TGG'CCA
Cuts at: 0 778 3109 4022 4566
Size: 778 2331 913 544
Fragments arranged by size:
2331 913 778 544
MslI CAynn'nnrTG
Cuts at: 0 286 436 3208 4387 4566
Size: 286 150 2772 1179 179
Fragments arranged by size:
2772 1179 286 179 150
MspA1I CmG'CkG
Cuts at: 0 1359 1486 2109 2190 2610 2640 2658 4197
Size: 1359 127 623 81 420 30 18 1539
Cuts at: 4197 4566
Size: 369
Fragments arranged by size:
1539 1359 623 420 369 127 81 30
18
NaeI GCC'GGC
Cuts at: 0 631 4566
Size: 631 3935
NarI GG'CG_CC
Cuts at: 0 335 4566
Size: 335 4231
NcoI C'CATG_G
Cuts at: 0 284 377 1412 3360 3662 4566
Size: 284 93 1035 1948 302 904
Fragments arranged by size:
1948 1035 904 302 284 93
NgoMIV G'CCGG_C
Cuts at: 0 629 4566
Size: 629 3937
NspI r_CATG'y
Cuts at: 0 532 4095 4566
Size: 532 3563 471
Fragments arranged by size:
3563 532 471
PasI CC'CwG_GG
Cuts at: 0 739 3740 3757 3772 4566
Size: 739 3001 17 15 794
Fragments arranged by size:
3001 794 739 17 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 598 939 1747 2481 4018 4488 4566
Size: 598 341 808 734 1537 470 78
Fragments arranged by size:
1537 808 734 598 470 341 78
PfoI T'CCnGG_A
Cuts at: 0 932 1103 2264 2396 4471 4566
Size: 932 171 1161 132 2075 95
Fragments arranged by size:
2075 1161 932 171 132 95
PmlI CAC'GTG
Cuts at: 0 2955 4566
Size: 2955 1611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1788 1820 4566
Size: 1788 32 2746
Fragments arranged by size:
2746 1788 32
PpuMI rG'GwC_Cy
Cuts at: 0 402 493 1323 1785 2262 2908 2970 3292
Size: 402 91 830 462 477 646 62 322
Cuts at: 3292 3401 3432 3444 3744 4102 4566
Size: 109 31 12 300 358 464
Fragments arranged by size:
830 646 477 464 462 402 358 322
300 109 91 62 31 12
PshAI GACnn'nnGTC
Cuts at: 0 1010 3839 4566
Size: 1010 2829 727
Fragments arranged by size:
2829 1010 727
PspOMI G'GGCC_C
Cuts at: 0 2723 3153 3315 4492 4566
Size: 2723 430 162 1177 74
Fragments arranged by size:
2723 1177 430 162 74
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4050 4082 4566
Size: 4050 32 484
Fragments arranged by size:
4050 484 32
PstI C_TGCA'G
Cuts at: 0 509 2609 4173 4202 4566
Size: 509 2100 1564 29 364
Fragments arranged by size:
2100 1564 509 364 29
PvuII CAG'CTG
Cuts at: 0 1359 1486 2109 2610 4197 4566
Size: 1359 127 623 501 1587 369
Fragments arranged by size:
1587 1359 623 501 369 127
SacI G_AGCT'C
Cuts at: 0 423 3218 4566
Size: 423 2795 1348
Fragments arranged by size:
2795 1348 423
SanDI GG'GwC_CC
Cuts at: 0 1323 2908 3401 3744 4566
Size: 1323 1585 493 343 822
Fragments arranged by size:
1585 1323 822 493 343
SapI GCTCTTCn'nnn_
Cuts at: 0 369 1543 1641 2338 2569 4566
Size: 369 1174 98 697 231 1997
Fragments arranged by size:
1997 1174 697 369 231 98
SbfI CC_TGCA'GG
Cuts at: 0 4173 4566
Size: 4173 393
SexAI A'CCwGG_T
Cuts at: 0 1981 3428 4566
Size: 1981 1447 1138
Fragments arranged by size:
1981 1447 1138
SfcI C'TryA_G
Cuts at: 0 505 1147 1254 2605 2986 4169 4198 4566
Size: 505 642 107 1351 381 1183 29 368
Fragments arranged by size:
1351 1183 642 505 381 368 107 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 784 4566
Size: 784 3782
SfoI GGC'GCC
Cuts at: 0 336 4566
Size: 336 4230
SmaI CCC'GGG
Cuts at: 0 899 2314 2728 3298 4566
Size: 899 1415 414 570 1268
Fragments arranged by size:
1415 1268 899 570 414
SmlI C'TyrA_G
Cuts at: 0 1211 1843 1990 2749 3076 4113 4300 4315
Size: 1211 632 147 759 327 1037 187 15
Cuts at: 4315 4348 4566
Size: 33 218
Fragments arranged by size:
1211 1037 759 632 327 218 187 147
33 15
SphI G_CATG'C
Cuts at: 0 4095 4566
Size: 4095 471
SspI AAT'ATT
Cuts at: 0 1098 4566
Size: 1098 3468
StyI C'CwwG_G
Cuts at: 0 284 377 1061 1368 1412 1580 2942 3360
Size: 284 93 684 307 44 168 1362 418
Cuts at: 3360 3417 3662 3868 4356 4566
Size: 57 245 206 488 210
Fragments arranged by size:
1362 684 488 418 307 284 245 210
206 168 93 57 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 550 3339 3472 3720 3808 4152 4566
Size: 550 2789 133 248 88 344 414
Fragments arranged by size:
2789 550 414 344 248 133 88
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 338 370 2246 2278 4566
Size: 338 32 1876 32 2288
Fragments arranged by size:
2288 1876 338 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2850 4030 4566
Size: 2850 1180 536
Fragments arranged by size:
2850 1180 536
XmaI C'CCGG_G
Cuts at: 0 897 2312 2726 3296 4566
Size: 897 1415 414 570 1270
Fragments arranged by size:
1415 1270 897 570 414
XmnI GAAnn'nnTTC
Cuts at: 0 686 1099 4566
Size: 686 413 3467
Fragments arranged by size:
3467 686 413
ZraI GAC'GTC
Cuts at: 0 3188 4566
Size: 3188 1378
Enzymes that do cut:
AarI AatII AcuI AflIII AgeI AhdI AleI AlfI
AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BclI BglI BglII Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HincII HindIII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuII SacI
SanDI SapI SbfI SexAI SfcI SfiI SfoI SmaI
SmlI SphI SspI StyI TaqII TstI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII ApaLI AscI AseI
AsiSI AvrII BaeI BcgI BciVI BfrBI BlpI BmtI
BsaBI BsiWI BsmBI BspEI BspHI BsrGI BssHII BssSI
BstBI BstZ17I ClaI CspCI DraI DraIII DrdI EagI
EcoRI EcoRV FseI FspAI HpaI KpnI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PsiI PspXI PvuI RsrII SacII SalI ScaI SgfI
SgrAI SnaBI SpeI SrfI StuI SwaI TatI Tth111I
XbaI XhoI