Restriction map for
pF1KA0381
(Linear) (Six-base) MAPSORT of: KIAA0381.seq Check: 6772 from: 1 to: 3204
KIAA0381.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AatII G_ACGT'C
Cuts at: 0 1772 3204
Size: 1772 1432
AccI GT'mk_AC
Cuts at: 0 924 3204
Size: 924 2280
Acc65I G'GTAC_C
Cuts at: 0 797 3204
Size: 797 2407
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1120 1834 1991 2564 2748 3004 3204
Size: 418 702 714 157 573 184 256 200
Fragments arranged by size:
714 702 573 418 256 200 184 157
AflIII A'CryG_T
Cuts at: 0 347 576 3204
Size: 347 229 2628
Fragments arranged by size:
2628 347 229
AhdI GACnn_n'nnGTC
Cuts at: 0 2493 3078 3204
Size: 2493 585 126
Fragments arranged by size:
2493 585 126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1750 3204
Size: 1750 1454
AlwNI CAG_nnn'CTG
Cuts at: 0 342 765 1200 1488 1552 2274 2605 3162
Size: 342 423 435 288 64 722 331 557
Cuts at: 3162 3204
Size: 42
Fragments arranged by size:
722 557 435 423 342 331 288 64
42
ApaI G_GGCC'C
Cuts at: 0 1582 2034 3204
Size: 1582 452 1170
Fragments arranged by size:
1582 1170 452
ApoI r'AATT_y
Cuts at: 0 474 907 2299 2866 3204
Size: 474 433 1392 567 338
Fragments arranged by size:
1392 567 474 433 338
AvaI C'yCGr_G
Cuts at: 0 1472 1499 1684 2357 3173 3204
Size: 1472 27 185 673 816 31
Fragments arranged by size:
1472 816 673 185 31 27
BamHI G'GATC_C
Cuts at: 0 1898 3204
Size: 1898 1306
BanI G'GyrC_C
Cuts at: 0 797 1708 1858 1946 2446 2696 3204
Size: 797 911 150 88 500 250 508
Fragments arranged by size:
911 797 508 500 250 150 88
BanII G_rGCy'C
Cuts at: 0 143 1582 1966 2034 2714 3204
Size: 143 1439 384 68 680 490
Fragments arranged by size:
1439 680 490 384 143 68
BbeI G_GCGC'C
Cuts at: 0 2450 2700 3204
Size: 2450 250 504
Fragments arranged by size:
2450 504 250
BbsI GAAGACnn'nnnn_
Cuts at: 0 398 406 1586 1979 3096 3204
Size: 398 8 1180 393 1117 108
Fragments arranged by size:
1180 1117 398 393 108 8
BbvCI CC'TCA_GC
Cuts at: 0 2318 3204
Size: 2318 886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1876 1910 3026 3060 3204
Size: 1876 34 1116 34 144
Fragments arranged by size:
1876 1116 144 34 34
BclI T'GATC_A
Cuts at: 0 2534 3204
Size: 2534 670
BglI GCCn_nnn'nGGC
Cuts at: 0 26 3204
Size: 26 3178
BglII A'GATC_T
Cuts at: 0 236 3204
Size: 236 2968
Bme1580I G_kGCm'C
Cuts at: 0 576 1582 1713 2034 3204
Size: 576 1006 131 321 1170
Fragments arranged by size:
1170 1006 576 321 131
BmrI ACTGGGnnnn_n'
Cuts at: 0 1836 2708 3204
Size: 1836 872 496
Fragments arranged by size:
1836 872 496
BmtI G_CTAG'C
Cuts at: 0 1027 3204
Size: 1027 2177
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1980 3204
Size: 1980 1224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 472 679 881 887 901 1039 1198
Size: 99 373 207 202 6 14 138 159
Cuts at: 1198 1213 1405 2158 2206 2566 2650 2704 2800
Size: 15 192 753 48 360 84 54 96
Cuts at: 2800 3070 3204
Size: 270 134
Fragments arranged by size:
753 373 360 270 207 202 192 159
138 134 99 96 84 54 48 15
14 6
Bpu10I CC'TnA_GC
Cuts at: 0 2126 2318 3204
Size: 2126 192 886
Fragments arranged by size:
2126 886 192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2134 3112 3204
Size: 2134 978 92
Fragments arranged by size:
2134 978 92
BsaI GGTCTCn'nnnn_
Cuts at: 0 1328 3204
Size: 1328 1876
BsaBI GATnn'nnATC
Cuts at: 0 2539 3204
Size: 2539 665
BsaHI Gr'CG_yC
Cuts at: 0 1769 2447 2697 3204
Size: 1769 678 250 507
Fragments arranged by size:
1769 678 507 250
BsaWI w'CCGG_w
Cuts at: 0 410 482 1352 3204
Size: 410 72 870 1852
Fragments arranged by size:
1852 870 410 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2671 2701 3204
Size: 2671 30 503
Fragments arranged by size:
2671 503 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 30 151 373 376 1622 1625 1858 2092
Size: 30 121 222 3 1246 3 233 234
Cuts at: 2092 2137 2203 2949 2952 2962 2965 2968 3067
Size: 45 66 746 3 10 3 3 99
Cuts at: 3067 3074 3204
Size: 7 130
Fragments arranged by size:
1246 746 234 233 222 130 121 99
66 45 30 10 7 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 918 1526 1759 1796 1959 3204
Size: 918 608 233 37 163 1245
Fragments arranged by size:
1245 918 608 233 163 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1123 3204
Size: 706 417 2081
Fragments arranged by size:
2081 706 417
BsiEI CG_ry'CG
Cuts at: 0 312 3204
Size: 312 2892
BsiHKAI G_wGCw'C
Cuts at: 0 143 2195 3204
Size: 143 2052 1009
Fragments arranged by size:
2052 1009 143
BsmI GAATG_Cn'
Cuts at: 0 1408 3204
Size: 1408 1796
BsmBI CGTCTCn'nnnn_
Cuts at: 0 358 3204
Size: 358 2846
Bsp1286I G_dGCh'C
Cuts at: 0 143 576 1582 1713 1966 2034 2195 2714
Size: 143 433 1006 131 253 68 161 519
Cuts at: 2714 3204
Size: 490
Fragments arranged by size:
1006 519 490 433 253 161 143 131
68
BspEI T'CCGG_A
Cuts at: 0 410 482 1352 3204
Size: 410 72 870 1852
Fragments arranged by size:
1852 870 410 72
BspHI T'CATG_A
Cuts at: 0 2423 2537 2735 3204
Size: 2423 114 198 469
Fragments arranged by size:
2423 469 198 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1625 1878 3204
Size: 1625 253 1326
Fragments arranged by size:
1625 1326 253
BsrBI CCG'CTC
Cuts at: 0 1239 1398 2211 2310 2668 2958 3006 3180
Size: 1239 159 813 99 358 290 48 174
Cuts at: 3180 3204
Size: 24
Fragments arranged by size:
1239 813 358 290 174 159 99 48
24
BsrFI r'CCGG_y
Cuts at: 0 794 3191 3204
Size: 794 2397 13
Fragments arranged by size:
2397 794 13
BssHII G'CGCG_C
Cuts at: 0 2965 3204
Size: 2965 239
BssSI C'ACGA_G
Cuts at: 0 682 3204
Size: 682 2522
BstAPI GCAn_nnn'nTGC
Cuts at: 0 717 2274 3204
Size: 717 1557 930
Fragments arranged by size:
1557 930 717
BstXI CCAn_nnnn'nTGG
Cuts at: 0 279 2349 3204
Size: 279 2070 855
Fragments arranged by size:
2070 855 279
BstYI r'GATC_y
Cuts at: 0 177 236 662 1898 2081 2919 3204
Size: 177 59 426 1236 183 838 285
Fragments arranged by size:
1236 838 426 285 183 177 59
BstZ17I GTA'TAC
Cuts at: 0 925 3204
Size: 925 2279
Bsu36I CC'TnA_GG
Cuts at: 0 593 1745 2650 3204
Size: 593 1152 905 554
Fragments arranged by size:
1152 905 593 554
BtgI C'CryG_G
Cuts at: 0 24 323 3204
Size: 24 299 2881
Fragments arranged by size:
2881 299 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 346 3204
Size: 346 2858
BtsI GCAGTG_nn'
Cuts at: 0 63 211 1143 1195 1770 3204
Size: 63 148 932 52 575 1434
Fragments arranged by size:
1434 932 575 148 63 52
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1571 1606 3204
Size: 1571 35 1598
Fragments arranged by size:
1598 1571 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2725 3204
Size: 2725 479
EaeI y'GGCC_r
Cuts at: 0 693 2022 2465 3204
Size: 693 1329 443 739
Fragments arranged by size:
1329 739 693 443
EarI CTCTTCn'nnn_
Cuts at: 0 1424 2161 2290 3204
Size: 1424 737 129 914
Fragments arranged by size:
1424 914 737 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 877 3204
Size: 877 2327
EcoICRI GAG'CTC
Cuts at: 0 141 3204
Size: 141 3063
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 418 472 679 881 887 901 1039
Size: 99 319 54 207 202 6 14 138
Cuts at: 1039 1120 1198 1213 1405 1834 1991 2158 2206
Size: 81 78 15 192 429 157 167 48
Cuts at: 2206 2564 2566 2650 2704 2748 2800 3004 3070
Size: 358 2 84 54 44 52 204 66
Cuts at: 3070 3204
Size: 134
Fragments arranged by size:
429 358 319 207 204 202 192 167
157 138 134 99 84 81 78 66
54 54 52 48 44 15 14 6
2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1744 1941 2125 2185 3204
Size: 1744 197 184 60 1019
Fragments arranged by size:
1744 1019 197 184 60
EcoO109I rG'GnC_Cy
Cuts at: 0 267 1549 1578 1690 1749 2030 2118 2658
Size: 267 1282 29 112 59 281 88 540
Cuts at: 2658 3027 3159 3204
Size: 369 132 45
Fragments arranged by size:
1282 540 369 281 267 132 112 88
59 45 29
EcoRI G'AATT_C
Cuts at: 0 907 2866 3204
Size: 907 1959 338
Fragments arranged by size:
1959 907 338
HaeII r_GCGC'y
Cuts at: 0 2450 2700 3204
Size: 2450 250 504
Fragments arranged by size:
2450 504 250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 324 356 2479 2511 3064 3096 3204
Size: 324 32 2123 32 553 32 108
Fragments arranged by size:
2123 553 324 108 32 32 32
HincII GTy'rAC
Cuts at: 0 1296 2616 3204
Size: 1296 1320 588
Fragments arranged by size:
1320 1296 588
HindIII A'AGCT_T
Cuts at: 0 2065 3204
Size: 2065 1139
KasI G'GCGC_C
Cuts at: 0 2446 2696 3204
Size: 2446 250 508
Fragments arranged by size:
2446 508 250
KpnI G_GTAC'C
Cuts at: 0 801 3204
Size: 801 2403
MluI A'CGCG_T
Cuts at: 0 347 3204
Size: 347 2857
MscI TGG'CCA
Cuts at: 0 695 2467 3204
Size: 695 1772 737
Fragments arranged by size:
1772 737 695
MslI CAynn'nnrTG
Cuts at: 0 959 2198 2752 3204
Size: 959 1239 554 452
Fragments arranged by size:
1239 959 554 452
MspA1I CmG'CkG
Cuts at: 0 290 3015 3021 3204
Size: 290 2725 6 183
Fragments arranged by size:
2725 290 183 6
NarI GG'CG_CC
Cuts at: 0 2447 2697 3204
Size: 2447 250 507
Fragments arranged by size:
2447 507 250
NcoI C'CATG_G
Cuts at: 0 24 323 3204
Size: 24 299 2881
Fragments arranged by size:
2881 299 24
NheI G'CTAG_C
Cuts at: 0 1023 3204
Size: 1023 2181
NspI r_CATG'y
Cuts at: 0 580 1302 1887 2136 2577 3204
Size: 580 722 585 249 441 627
Fragments arranged by size:
722 627 585 580 441 249
PasI CC'CwG_GG
Cuts at: 0 564 1573 3204
Size: 564 1009 1631
Fragments arranged by size:
1631 1009 564
PciI A'CATG_T
Cuts at: 0 576 3204
Size: 576 2628
PflMI CCAn_nnn'nTGG
Cuts at: 0 30 387 1573 3162 3204
Size: 30 357 1186 1589 42
Fragments arranged by size:
1589 1186 357 42 30
PfoI T'CCnGG_A
Cuts at: 0 80 968 1199 1229 1901 2183 2303 2543
Size: 80 888 231 30 672 282 120 240
Cuts at: 2543 3204
Size: 661
Fragments arranged by size:
888 672 661 282 240 231 120 80
30
PpuMI rG'GwC_Cy
Cuts at: 0 267 1690 1749 2118 3027 3204
Size: 267 1423 59 369 909 177
Fragments arranged by size:
1423 909 369 267 177 59
PshAI GACnn'nnGTC
Cuts at: 0 785 3204
Size: 785 2419
PspOMI G'GGCC_C
Cuts at: 0 1578 2030 3204
Size: 1578 452 1174
Fragments arranged by size:
1578 1174 452
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1162 1194 3204
Size: 1162 32 2010
Fragments arranged by size:
2010 1162 32
PstI C_TGCA'G
Cuts at: 0 101 247 713 1154 3039 3204
Size: 101 146 466 441 1885 165
Fragments arranged by size:
1885 466 441 165 146 101
PvuII CAG'CTG
Cuts at: 0 290 3204
Size: 290 2914
SacI G_AGCT'C
Cuts at: 0 143 3204
Size: 143 3061
SanDI GG'GwC_CC
Cuts at: 0 1690 3204
Size: 1690 1514
SexAI A'CCwGG_T
Cuts at: 0 728 3074 3204
Size: 728 2346 130
Fragments arranged by size:
2346 728 130
SfcI C'TryA_G
Cuts at: 0 97 243 709 1150 3035 3204
Size: 97 146 466 441 1885 169
Fragments arranged by size:
1885 466 441 169 146 97
SfoI GGC'GCC
Cuts at: 0 2448 2698 3204
Size: 2448 250 506
Fragments arranged by size:
2448 506 250
SmaI CCC'GGG
Cuts at: 0 1474 1686 2359 3175 3204
Size: 1474 212 673 816 29
Fragments arranged by size:
1474 816 673 212 29
SmlI C'TyrA_G
Cuts at: 0 2149 3127 3204
Size: 2149 978 77
Fragments arranged by size:
2149 978 77
StuI AGG'CCT
Cuts at: 0 1114 2821 2899 3204
Size: 1114 1707 78 305
Fragments arranged by size:
1707 1114 305 78
StyI C'CwwG_G
Cuts at: 0 24 323 644 1265 2816 3204
Size: 24 299 321 621 1551 388
Fragments arranged by size:
1551 621 388 321 299 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 658 797 1818 1823 2001 3204
Size: 658 139 1021 5 178 1203
Fragments arranged by size:
1203 1021 658 178 139 5
TatI w'GTAC_w
Cuts at: 0 1103 3204
Size: 1103 2101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1773 1805 2205 2237 3204
Size: 1773 32 400 32 967
Fragments arranged by size:
1773 967 400 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1042 3204
Size: 1042 2162
XbaI T'CTAG_A
Cuts at: 0 2922 3204
Size: 2922 282
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2100 3204
Size: 2100 1104
XmaI C'CCGG_G
Cuts at: 0 1472 1684 2357 3173 3204
Size: 1472 212 673 816 31
Fragments arranged by size:
1472 816 673 212 31
XmnI GAAnn'nnTTC
Cuts at: 0 1819 2299 3204
Size: 1819 480 905
Fragments arranged by size:
1819 905 480
ZraI GAC'GTC
Cuts at: 0 1770 3204
Size: 1770 1434
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AhdI AlfI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BclI BglI BglII Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI HaeII Hin4I HincII HindIII KasI KpnI
MluI MscI MslI MspA1I NarI NcoI NheI NspI
PasI PciI PflMI PfoI PpuMI PshAI PspOMI PsrI
PstI PvuII SacI SanDI SexAI SfcI SfoI SmaI
SmlI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AleI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BciVI BfrBI BlpI
BmgBI BsaAI BsiWI BsrDI BsrGI BstBI BstEII ClaI
DraI DraIII EagI EcoRV FalI FseI FspI FspAI
HpaI MfeI MmeI NaeI NdeI NgoMIV NotI NruI
NsiI PacI PmeI PmlI PpiI PsiI PspXI PvuI
RsrII SacII SalI SapI SbfI ScaI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI XhoI