Restriction map for
pF1KSDA0383
(Linear) (Six-base) MAPSORT of: KIAA0383.seq Check: 5699 from: 1 to: 5343
KIAA0383.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2238 5343
Size: 2238 3105
AccI GT'mk_AC
Cuts at: 0 3560 4112 5343
Size: 3560 552 1231
Fragments arranged by size:
3560 1231 552
Acc65I G'GTAC_C
Cuts at: 0 1277 5343
Size: 1277 4066
AclI AA'CG_TT
Cuts at: 0 1570 5343
Size: 1570 3773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 397 1211 2138 2993 3632 4551 5343
Size: 104 293 814 927 855 639 919 792
Fragments arranged by size:
927 919 855 814 792 639 293 104
AflII C'TTAA_G
Cuts at: 0 783 5343
Size: 783 4560
AflIII A'CryG_T
Cuts at: 0 3109 5343
Size: 3109 2234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2246 4342 5003 5343
Size: 2246 2096 661 340
Fragments arranged by size:
2246 2096 661 340
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2180 2212 4219 4251 5343
Size: 2180 32 2007 32 1092
Fragments arranged by size:
2180 2007 1092 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 142 453 821 2116 2307 4205 4419 4930
Size: 142 311 368 1295 191 1898 214 511
Cuts at: 4930 5343
Size: 413
Fragments arranged by size:
1898 1295 511 413 368 311 214 191
142
ApoI r'AATT_y
Cuts at: 0 748 1294 1378 1463 1517 2937 5343
Size: 748 546 84 85 54 1420 2406
Fragments arranged by size:
2406 1420 748 546 85 84 54
AvaI C'yCGr_G
Cuts at: 0 4477 5343
Size: 4477 866
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 541 574 5343
Size: 541 33 4769
Fragments arranged by size:
4769 541 33
BamHI G'GATC_C
Cuts at: 0 1081 5343
Size: 1081 4262
BanI G'GyrC_C
Cuts at: 0 549 1277 5209 5343
Size: 549 728 3932 134
Fragments arranged by size:
3932 728 549 134
BanII G_rGCy'C
Cuts at: 0 2600 3590 5343
Size: 2600 990 1753
Fragments arranged by size:
2600 1753 990
BbsI GAAGACnn'nnnn_
Cuts at: 0 139 1271 2926 3329 5343
Size: 139 1132 1655 403 2014
Fragments arranged by size:
2014 1655 1132 403 139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3672 3706 5343
Size: 3672 34 1637
Fragments arranged by size:
3672 1637 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 569 1639 5343
Size: 569 1070 3704
Fragments arranged by size:
3704 1070 569
BclI T'GATC_A
Cuts at: 0 1700 3342 4608 5343
Size: 1700 1642 1266 735
Fragments arranged by size:
1700 1642 1266 735
BfrBI ATG'CAT
Cuts at: 0 1093 5343
Size: 1093 4250
BlpI GC'TnA_GC
Cuts at: 0 2147 5343
Size: 2147 3196
Bme1580I G_kGCm'C
Cuts at: 0 552 1927 3117 5212 5343
Size: 552 1375 1190 2095 131
Fragments arranged by size:
2095 1375 1190 552 131
BmrI ACTGGGnnnn_n'
Cuts at: 0 469 557 3023 3074 3913 5343
Size: 469 88 2466 51 839 1430
Fragments arranged by size:
2466 1430 839 469 88 51
BmtI G_CTAG'C
Cuts at: 0 2171 5343
Size: 2171 3172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1436 2329 2659 4239 5343
Size: 1436 893 330 1580 1104
Fragments arranged by size:
1580 1436 1104 893 330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 3068 3803 4361 5343
Size: 373 2695 735 558 982
Fragments arranged by size:
2695 982 735 558 373
BsaI GGTCTCn'nnnn_
Cuts at: 0 1793 2557 5343
Size: 1793 764 2786
Fragments arranged by size:
2786 1793 764
BsaHI Gr'CG_yC
Cuts at: 0 3557 5343
Size: 3557 1786
BsaWI w'CCGG_w
Cuts at: 0 2923 5343
Size: 2923 2420
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3239 3269 4219 4237 4249 4267 4767 4797
Size: 3239 30 950 18 12 18 500 30
Cuts at: 4797 5343
Size: 546
Fragments arranged by size:
3239 950 546 500 30 30 18 18
12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 360 370 691 1317 2356 2359 2362 2371
Size: 360 10 321 626 1039 3 3 9
Cuts at: 2371 2374 2386 2413 3061 3064 3202 3205 3208
Size: 3 12 27 648 3 138 3 3
Cuts at: 3208 3211 3260 3367 3370 3649 4364 4769 4772
Size: 3 49 107 3 279 715 405 3
Cuts at: 4772 5343
Size: 571
Fragments arranged by size:
1039 715 648 626 571 405 360 321
279 138 107 49 27 12 10 9
3 3 3 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 435 1709 2174 3601 3717 4289 4537 5117
Size: 435 1274 465 1427 116 572 248 580
Cuts at: 5117 5213 5225 5343
Size: 96 12 118
Fragments arranged by size:
1427 1274 580 572 465 435 248 118
116 96 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 731 977 1172 3314 3535 3875 4449 4973
Size: 731 246 195 2142 221 340 574 524
Cuts at: 4973 5343
Size: 370
Fragments arranged by size:
2142 731 574 524 370 340 246 221
195
BsiEI CG_ry'CG
Cuts at: 0 1033 5343
Size: 1033 4310
BsiHKAI G_wGCw'C
Cuts at: 0 4150 5343
Size: 4150 1193
BsiWI C'GTAC_G
Cuts at: 0 3954 5343
Size: 3954 1389
BsmI GAATG_Cn'
Cuts at: 0 568 808 898 923 1093 1095 2262 2698
Size: 568 240 90 25 170 2 1167 436
Cuts at: 2698 2775 5343
Size: 77 2568
Fragments arranged by size:
2568 1167 568 436 240 170 90 77
25 2
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1825 2569 4474 4919 5343
Size: 1825 744 1905 445 424
Fragments arranged by size:
1905 1825 744 445 424
Bsp1286I G_dGCh'C
Cuts at: 0 552 1927 2600 3117 3590 4150 5212 5343
Size: 552 1375 673 517 473 560 1062 131
Fragments arranged by size:
1375 1062 673 560 552 517 473 131
BspHI T'CATG_A
Cuts at: 0 981 5343
Size: 981 4362
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2238 2970 4366 5343
Size: 2238 732 1396 977
Fragments arranged by size:
2238 1396 977 732
BsrBI CCG'CTC
Cuts at: 0 2151 5343
Size: 2151 3192
BsrDI GCAATG_nn'
Cuts at: 0 315 1934 2757 4615 5343
Size: 315 1619 823 1858 728
Fragments arranged by size:
1858 1619 823 728 315
BsrFI r'CCGG_y
Cuts at: 0 1274 2276 5343
Size: 1274 1002 3067
Fragments arranged by size:
3067 1274 1002
BsrGI T'GTAC_A
Cuts at: 0 2942 5264 5343
Size: 2942 2322 79
Fragments arranged by size:
2942 2322 79
BstAPI GCAn_nnn'nTGC
Cuts at: 0 453 803 812 4660 5235 5343
Size: 453 350 9 3848 575 108
Fragments arranged by size:
3848 575 453 350 108 9
BstEII G'GTnAC_C
Cuts at: 0 1109 5343
Size: 1109 4234
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4464 5343
Size: 4464 879
BstYI r'GATC_y
Cuts at: 0 1081 3264 5343
Size: 1081 2183 2079
Fragments arranged by size:
2183 2079 1081
Bsu36I CC'TnA_GG
Cuts at: 0 3173 5343
Size: 3173 2170
BtgI C'CryG_G
Cuts at: 0 117 2963 3311 3725 5250 5343
Size: 117 2846 348 414 1525 93
Fragments arranged by size:
2846 1525 414 348 117 93
BtsI GCAGTG_nn'
Cuts at: 0 799 822 2117 2334 2556 5343
Size: 799 23 1295 217 222 2787
Fragments arranged by size:
2787 1295 799 222 217 23
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1416 1451 5343
Size: 1416 35 3892
Fragments arranged by size:
3892 1416 35
DraI TTT'AAA
Cuts at: 0 2931 5343
Size: 2931 2412
DraIII CAC_nnn'GTG
Cuts at: 0 3142 5343
Size: 3142 2201
DrdI GACnn_nn'nnGTC
Cuts at: 0 3556 5343
Size: 3556 1787
EaeI y'GGCC_r
Cuts at: 0 1272 2278 2866 3945 5343
Size: 1272 1006 588 1079 1398
Fragments arranged by size:
1398 1272 1079 1006 588
EarI CTCTTCn'nnn_
Cuts at: 0 80 1187 1558 1764 1837 2117 2333 2363
Size: 80 1107 371 206 73 280 216 30
Cuts at: 2363 2375 2381 2390 2402 2612 2732 3173 3179
Size: 12 6 9 12 210 120 441 6
Cuts at: 3179 3197 3206 3215 3344 3356 3365 3443 4224
Size: 18 9 9 129 12 9 78 781
Cuts at: 4224 5343
Size: 1119
Fragments arranged by size:
1119 1107 781 441 371 280 216 210
206 129 120 80 78 73 30 18
12 12 12 9 9 9 9 6
6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 397 1211 1436 2138 2329 2659 2993
Size: 104 293 814 225 702 191 330 334
Cuts at: 2993 3632 4239 4551 5343
Size: 639 607 312 792
Fragments arranged by size:
814 792 702 639 607 334 330 312
293 225 191 104
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1654 5343
Size: 1654 3689
EcoO109I rG'GnC_Cy
Cuts at: 0 77 216 3606 4735 4984 5343
Size: 77 139 3390 1129 249 359
Fragments arranged by size:
3390 1129 359 249 139 77
EcoRI G'AATT_C
Cuts at: 0 1378 5343
Size: 1378 3965
HaeII r_GCGC'y
Cuts at: 0 237 4962 5047 5343
Size: 237 4725 85 296
Fragments arranged by size:
4725 296 237 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1066 1098 1724 1756 1976 2008 4066 4098
Size: 1066 32 626 32 220 32 2058 32
Cuts at: 4098 4187 4219 4767 4799 5343
Size: 89 32 548 32 544
Fragments arranged by size:
2058 1066 626 548 544 220 89 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2204 3561 3966 4592 5061 5343
Size: 2204 1357 405 626 469 282
Fragments arranged by size:
2204 1357 626 469 405 282
HindIII A'AGCT_T
Cuts at: 0 1363 1663 3465 5343
Size: 1363 300 1802 1878
Fragments arranged by size:
1878 1802 1363 300
HpaI GTT'AAC
Cuts at: 0 4592 5343
Size: 4592 751
KpnI G_GTAC'C
Cuts at: 0 1281 5343
Size: 1281 4062
MfeI C'AATT_G
Cuts at: 0 341 5343
Size: 341 5002
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 1623 3884 4325 4378 5343
Size: 104 1519 2261 441 53 965
Fragments arranged by size:
2261 1519 965 441 104 53
MscI TGG'CCA
Cuts at: 0 2868 3947 5343
Size: 2868 1079 1396
Fragments arranged by size:
2868 1396 1079
MslI CAynn'nnrTG
Cuts at: 0 1874 4033 4894 5020 5026 5077 5302 5343
Size: 1874 2159 861 126 6 51 225 41
Fragments arranged by size:
2159 1874 861 225 126 51 41 6
MspA1I CmG'CkG
Cuts at: 0 150 450 2304 2637 4232 4277 4310 4419
Size: 150 300 1854 333 1595 45 33 109
Cuts at: 4419 4464 4636 4930 5343
Size: 45 172 294 413
Fragments arranged by size:
1854 1595 413 333 300 294 172 150
109 45 45 33
NaeI GCC'GGC
Cuts at: 0 2278 5343
Size: 2278 3065
NcoI C'CATG_G
Cuts at: 0 117 2963 3725 5343
Size: 117 2846 762 1618
Fragments arranged by size:
2846 1618 762 117
NdeI CA'TA_TG
Cuts at: 0 887 5220 5343
Size: 887 4333 123
Fragments arranged by size:
4333 887 123
NgoMIV G'CCGG_C
Cuts at: 0 2276 5343
Size: 2276 3067
NheI G'CTAG_C
Cuts at: 0 2167 5343
Size: 2167 3176
NsiI A_TGCA'T
Cuts at: 0 1095 5343
Size: 1095 4248
NspI r_CATG'y
Cuts at: 0 815 1923 3113 4326 5115 5307 5343
Size: 815 1108 1190 1213 789 192 36
Fragments arranged by size:
1213 1190 1108 815 789 192 36
PasI CC'CwG_GG
Cuts at: 0 229 4729 5343
Size: 229 4500 614
Fragments arranged by size:
4500 614 229
PciI A'CATG_T
Cuts at: 0 3109 5343
Size: 3109 2234
PflMI CCAn_nnn'nTGG
Cuts at: 0 123 4419 5343
Size: 123 4296 924
Fragments arranged by size:
4296 924 123
PfoI T'CCnGG_A
Cuts at: 0 1556 5343
Size: 1556 3787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4219 4251 5343
Size: 4219 32 1092
Fragments arranged by size:
4219 1092 32
PpuMI rG'GwC_Cy
Cuts at: 0 216 3606 4735 4984 5343
Size: 216 3390 1129 249 359
Fragments arranged by size:
3390 1129 359 249 216
PshAI GACnn'nnGTC
Cuts at: 0 3863 5343
Size: 3863 1480
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 5122 5154 5343
Size: 5122 32 189
Fragments arranged by size:
5122 189 32
PstI C_TGCA'G
Cuts at: 0 952 1952 2112 4049 4321 4331 4363 4586
Size: 952 1000 160 1937 272 10 32 223
Cuts at: 4586 5343
Size: 757
Fragments arranged by size:
1937 1000 952 757 272 223 160 32
10
PvuII CAG'CTG
Cuts at: 0 150 2304 2637 4232 4277 4419 4464 5343
Size: 150 2154 333 1595 45 142 45 879
Fragments arranged by size:
2154 1595 879 333 150 142 45 45
RsrII CG'GwC_CG
Cuts at: 0 505 5343
Size: 505 4838
SalI G'TCGA_C
Cuts at: 0 3559 5343
Size: 3559 1784
SanDI GG'GwC_CC
Cuts at: 0 3606 4735 5343
Size: 3606 1129 608
Fragments arranged by size:
3606 1129 608
SapI GCTCTTCn'nnn_
Cuts at: 0 1558 1837 2117 3365 3443 4224 5343
Size: 1558 279 280 1248 78 781 1119
Fragments arranged by size:
1558 1248 1119 781 280 279 78
ScaI AGT'ACT
Cuts at: 0 111 2588 3479 5343
Size: 111 2477 891 1864
Fragments arranged by size:
2477 1864 891 111
SexAI A'CCwGG_T
Cuts at: 0 1318 3138 5343
Size: 1318 1820 2205
Fragments arranged by size:
2205 1820 1318
SfcI C'TryA_G
Cuts at: 0 822 948 1948 2108 3828 3838 4045 4239
Size: 822 126 1000 160 1720 10 207 194
Cuts at: 4239 4317 4327 4359 4582 4970 5343
Size: 78 10 32 223 388 373
Fragments arranged by size:
1720 1000 822 388 373 223 207 194
160 126 78 32 10 10
SmlI C'TyrA_G
Cuts at: 0 352 783 3083 3818 4376 5343
Size: 352 431 2300 735 558 967
Fragments arranged by size:
2300 967 735 558 431 352
SphI G_CATG'C
Cuts at: 0 4326 5343
Size: 4326 1017
SspI AAT'ATT
Cuts at: 0 1410 2257 2439 4836 5343
Size: 1410 847 182 2397 507
Fragments arranged by size:
2397 1410 847 507 182
StuI AGG'CCT
Cuts at: 0 200 781 2499 3826 4398 5343
Size: 200 581 1718 1327 572 945
Fragments arranged by size:
1718 1327 945 581 572 200
StyI C'CwwG_G
Cuts at: 0 117 275 831 2963 3590 3725 5343
Size: 117 158 556 2132 627 135 1618
Fragments arranged by size:
2132 1618 627 556 158 135 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 749 1047 2976 4303 4470 4951 5227 5343
Size: 749 298 1929 1327 167 481 276 116
Fragments arranged by size:
1929 1327 749 481 298 276 167 116
TatI w'GTAC_w
Cuts at: 0 109 512 2238 2505 2586 2942 3477 5264
Size: 109 403 1726 267 81 356 535 1787
Cuts at: 5264 5343
Size: 79
Fragments arranged by size:
1787 1726 535 403 356 267 109 81
79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4709 4741 5343
Size: 4709 32 602
Fragments arranged by size:
4709 602 32
XbaI T'CTAG_A
Cuts at: 0 283 1765 5343
Size: 283 1482 3578
Fragments arranged by size:
3578 1482 283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 929 1554 2710 3940 4068 5149 5343
Size: 929 625 1156 1230 128 1081 194
Fragments arranged by size:
1230 1156 1081 929 625 194 128
XmnI GAAnn'nnTTC
Cuts at: 0 1093 2438 5343
Size: 1093 1345 2905
Fragments arranged by size:
2905 1345 1093
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AlfI
AloI AlwNI ApoI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BlpI Bme1580I BmrI
BmtI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI CspCI DraI DraIII
DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NsiI
NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI
PsrI PstI PvuII RsrII SalI SanDI SapI ScaI
SexAI SfcI SmlI SphI SspI StuI StyI TaqII
TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AleI ApaI ApaLI AscI
AseI AsiSI AvrII BbeI BbvCI BglI BglII BmgBI
BplI Bpu10I BsaAI BsaBI BspEI BssHII BssSI BstBI
BstZ17I BtgZI ClaI EagI EciI EcoICRI EcoRV FalI
FseI FspI FspAI KasI MluI NarI NotI NruI
PacI PmeI PmlI PsiI PspOMI PspXI PvuI SacI
SacII SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SwaI Tth111I XhoI XmaI ZraI