Restriction map for
pF1KA0384
(Linear) (Six-base) MAPSORT of: KIAA0384.seq Check: 7290 from: 1 to: 2817
KIAA0384.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AccI GT'mk_AC
Cuts at: 0 22 756 2817
Size: 22 734 2061
Fragments arranged by size:
2061 734 22
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1427 2817
Size: 1427 1390
AflII C'TTAA_G
Cuts at: 0 2014 2817
Size: 2014 803
AflIII A'CryG_T
Cuts at: 0 126 2817
Size: 126 2691
AhdI GACnn_n'nnGTC
Cuts at: 0 533 2093 2817
Size: 533 1560 724
Fragments arranged by size:
1560 724 533
ApaI G_GGCC'C
Cuts at: 0 776 2817
Size: 776 2041
ApaLI G'TGCA_C
Cuts at: 0 1270 2817
Size: 1270 1547
ApoI r'AATT_y
Cuts at: 0 235 2304 2660 2817
Size: 235 2069 356 157
Fragments arranged by size:
2069 356 235 157
AseI AT'TA_AT
Cuts at: 0 2236 2817
Size: 2236 581
AvaI C'yCGr_G
Cuts at: 0 80 695 1958 2817
Size: 80 615 1263 859
Fragments arranged by size:
1263 859 615 80
AvrII C'CTAG_G
Cuts at: 0 607 2817
Size: 607 2210
BamHI G'GATC_C
Cuts at: 0 345 1911 2817
Size: 345 1566 906
Fragments arranged by size:
1566 906 345
BanI G'GyrC_C
Cuts at: 0 101 747 947 984 2817
Size: 101 646 200 37 1833
Fragments arranged by size:
1833 646 200 101 37
BanII G_rGCy'C
Cuts at: 0 776 1011 1293 1973 2817
Size: 776 235 282 680 844
Fragments arranged by size:
844 776 680 282 235
BbeI G_GCGC'C
Cuts at: 0 105 951 2817
Size: 105 846 1866
Fragments arranged by size:
1866 846 105
BbsI GAAGACnn'nnnn_
Cuts at: 0 442 971 1207 1547 2817
Size: 442 529 236 340 1270
Fragments arranged by size:
1270 529 442 340 236
BbvCI CC'TCA_GC
Cuts at: 0 2049 2817
Size: 2049 768
BclI T'GATC_A
Cuts at: 0 1502 2420 2817
Size: 1502 918 397
Fragments arranged by size:
1502 918 397
BglI GCCn_nnn'nGGC
Cuts at: 0 840 2817
Size: 840 1977
Bme1580I G_kGCm'C
Cuts at: 0 750 776 1274 2817
Size: 750 26 498 1543
Fragments arranged by size:
1543 750 498 26
BmgBI CAC'GTC
Cuts at: 0 108 1209 1315 2817
Size: 108 1101 106 1502
Fragments arranged by size:
1502 1101 108 106
BmrI ACTGGGnnnn_n'
Cuts at: 0 57 932 1014 1225 1841 2817
Size: 57 875 82 211 616 976
Fragments arranged by size:
976 875 616 211 82 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1646 1902 1973 2045 2817
Size: 1646 256 71 72 772
Fragments arranged by size:
1646 772 256 72 71
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1872 2075 2213 2817
Size: 109 1763 203 138 604
Fragments arranged by size:
1763 604 203 138 109
Bpu10I CC'TnA_GC
Cuts at: 0 2049 2817
Size: 2049 768
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1204 1279 2817
Size: 1204 75 1538
Fragments arranged by size:
1538 1204 75
BsaI GGTCTCn'nnnn_
Cuts at: 0 141 322 373 406 2726 2817
Size: 141 181 51 33 2320 91
Fragments arranged by size:
2320 181 141 91 51 33
BsaBI GATnn'nnATC
Cuts at: 0 659 815 2002 2817
Size: 659 156 1187 815
Fragments arranged by size:
1187 815 659 156
BsaHI Gr'CG_yC
Cuts at: 0 102 948 2817
Size: 102 846 1869
Fragments arranged by size:
1869 846 102
BsaWI w'CCGG_w
Cuts at: 0 1417 2621 2817
Size: 1417 1204 196
Fragments arranged by size:
1417 1204 196
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 355 727 994 1635 2286 2744 2817
Size: 106 249 372 267 641 651 458 73
Fragments arranged by size:
651 641 458 372 267 249 106 73
BseYI C'CCAG_C
Cuts at: 0 727 1019 2077 2817
Size: 727 292 1058 740
Fragments arranged by size:
1058 740 727 292
BsiEI CG_ry'CG
Cuts at: 0 26 201 2758 2817
Size: 26 175 2557 59
Fragments arranged by size:
2557 175 59 26
BsiHKAI G_wGCw'C
Cuts at: 0 1274 1293 1580 1973 2199 2817
Size: 1274 19 287 393 226 618
Fragments arranged by size:
1274 618 393 287 226 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 114 2817
Size: 114 2703
Bsp1286I G_dGCh'C
Cuts at: 0 750 776 1011 1274 1293 1580 1973 2199
Size: 750 26 235 263 19 287 393 226
Cuts at: 2199 2817
Size: 618
Fragments arranged by size:
750 618 393 287 263 235 226 26
19
BspHI T'CATG_A
Cuts at: 0 953 2817
Size: 953 1864
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1175 2817
Size: 1175 1642
BsrBI CCG'CTC
Cuts at: 0 717 1527 2108 2441 2817
Size: 717 810 581 333 376
Fragments arranged by size:
810 717 581 376 333
BsrDI GCAATG_nn'
Cuts at: 0 564 1194 2817
Size: 564 630 1623
Fragments arranged by size:
1623 630 564
BsrFI r'CCGG_y
Cuts at: 0 185 200 2817
Size: 185 15 2617
Fragments arranged by size:
2617 185 15
BssSI C'ACGA_G
Cuts at: 0 1388 2817
Size: 1388 1429
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1483 2817
Size: 1483 1334
BstBI TT'CG_AA
Cuts at: 0 1380 2817
Size: 1380 1437
BstEII G'GTnAC_C
Cuts at: 0 699 2817
Size: 699 2118
BstXI CCAn_nnnn'nTGG
Cuts at: 0 36 2817
Size: 36 2781
BstYI r'GATC_y
Cuts at: 0 345 810 1778 1911 2763 2817
Size: 345 465 968 133 852 54
Fragments arranged by size:
968 852 465 345 133 54
Bsu36I CC'TnA_GG
Cuts at: 0 350 1600 2817
Size: 350 1250 1217
Fragments arranged by size:
1250 1217 350
BtgI C'CryG_G
Cuts at: 0 416 2817
Size: 416 2401
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 227 802 2817
Size: 227 575 2015
Fragments arranged by size:
2015 575 227
BtsI GCAGTG_nn'
Cuts at: 0 593 678 908 1477 2817
Size: 593 85 230 569 1340
Fragments arranged by size:
1340 593 569 230 85
ClaI AT'CG_AT
Cuts at: 0 2754 2817
Size: 2754 63
EaeI y'GGCC_r
Cuts at: 0 164 198 2817
Size: 164 34 2619
Fragments arranged by size:
2619 164 34
EagI C'GGCC_G
Cuts at: 0 198 2817
Size: 198 2619
EcoICRI GAG'CTC
Cuts at: 0 1291 1971 2817
Size: 1291 680 846
Fragments arranged by size:
1291 846 680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1427 1872 2075 2213 2817
Size: 109 1318 445 203 138 604
Fragments arranged by size:
1318 604 445 203 138 109
EcoO109I rG'GnC_Cy
Cuts at: 0 565 773 1069 2817
Size: 565 208 296 1748
Fragments arranged by size:
1748 565 296 208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1291 2817
Size: 1291 1526
FspI TGC'GCA
Cuts at: 0 1060 2817
Size: 1060 1757
HaeII r_GCGC'y
Cuts at: 0 88 105 951 2085 2817
Size: 88 17 846 1134 732
Fragments arranged by size:
1134 846 732 88 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 487 519 551 973 1005 1494
Size: 4 32 451 32 32 422 32 489
Cuts at: 1494 1526 1555 1587 2079 2111 2817
Size: 32 29 32 492 32 706
Fragments arranged by size:
706 492 489 451 422 32 32 32
32 32 32 32 29 4
HincII GTy'rAC
Cuts at: 0 23 2817
Size: 23 2794
HindIII A'AGCT_T
Cuts at: 0 1044 1720 2383 2817
Size: 1044 676 663 434
Fragments arranged by size:
1044 676 663 434
KasI G'GCGC_C
Cuts at: 0 101 947 2817
Size: 101 846 1870
Fragments arranged by size:
1870 846 101
MluI A'CGCG_T
Cuts at: 0 126 2817
Size: 126 2691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 2817
Size: 898 1919
MscI TGG'CCA
Cuts at: 0 166 2817
Size: 166 2651
MslI CAynn'nnrTG
Cuts at: 0 2591 2817
Size: 2591 226
MspA1I CmG'CkG
Cuts at: 0 120 1159 1591 2053 2817
Size: 120 1039 432 462 764
Fragments arranged by size:
1039 764 462 432 120
NaeI GCC'GGC
Cuts at: 0 187 2817
Size: 187 2630
NarI GG'CG_CC
Cuts at: 0 102 948 2817
Size: 102 846 1869
Fragments arranged by size:
1869 846 102
NdeI CA'TA_TG
Cuts at: 0 2592 2817
Size: 2592 225
NgoMIV G'CCGG_C
Cuts at: 0 185 2817
Size: 185 2632
NspI r_CATG'y
Cuts at: 0 1486 1843 2251 2817
Size: 1486 357 408 566
Fragments arranged by size:
1486 566 408 357
PflMI CCAn_nnn'nTGG
Cuts at: 0 531 2817
Size: 531 2286
PpuMI rG'GwC_Cy
Cuts at: 0 565 2817
Size: 565 2252
PshAI GACnn'nnGTC
Cuts at: 0 418 941 2817
Size: 418 523 1876
Fragments arranged by size:
1876 523 418
PspOMI G'GGCC_C
Cuts at: 0 772 2817
Size: 772 2045
PspXI vC'TCGA_Gb
Cuts at: 0 1958 2817
Size: 1958 859
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 604 636 2817
Size: 604 32 2181
Fragments arranged by size:
2181 604 32
PvuI CG_AT'CG
Cuts at: 0 2758 2817
Size: 2758 59
PvuII CAG'CTG
Cuts at: 0 120 1159 1591 2053 2817
Size: 120 1039 432 462 764
Fragments arranged by size:
1039 764 462 432 120
SacI G_AGCT'C
Cuts at: 0 1293 1973 2817
Size: 1293 680 844
Fragments arranged by size:
1293 844 680
SalI G'TCGA_C
Cuts at: 0 21 2817
Size: 21 2796
ScaI AGT'ACT
Cuts at: 0 1235 2604 2817
Size: 1235 1369 213
Fragments arranged by size:
1369 1235 213
SexAI A'CCwGG_T
Cuts at: 0 567 2817
Size: 567 2250
SfcI C'TryA_G
Cuts at: 0 365 1904 2817
Size: 365 1539 913
Fragments arranged by size:
1539 913 365
SfoI GGC'GCC
Cuts at: 0 103 949 2817
Size: 103 846 1868
Fragments arranged by size:
1868 846 103
SmaI CCC'GGG
Cuts at: 0 82 697 2817
Size: 82 615 2120
Fragments arranged by size:
2120 615 82
SmlI C'TyrA_G
Cuts at: 0 1219 1294 1958 2014 2817
Size: 1219 75 664 56 803
Fragments arranged by size:
1219 803 664 75 56
SphI G_CATG'C
Cuts at: 0 1486 2817
Size: 1486 1331
StyI C'CwwG_G
Cuts at: 0 607 1275 2817
Size: 607 668 1542
Fragments arranged by size:
1542 668 607
TatI w'GTAC_w
Cuts at: 0 456 1233 2602 2817
Size: 456 777 1369 215
Fragments arranged by size:
1369 777 456 215
Tth111I GACn'n_nGTC
Cuts at: 0 569 2320 2817
Size: 569 1751 497
Fragments arranged by size:
1751 569 497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 159 652 1082 2817
Size: 159 493 430 1735
Fragments arranged by size:
1735 493 430 159
XhoI C'TCGA_G
Cuts at: 0 1958 2817
Size: 1958 859
XmaI C'CCGG_G
Cuts at: 0 80 695 2817
Size: 80 615 2122
Fragments arranged by size:
2122 615 80
XmnI GAAnn'nnTTC
Cuts at: 0 2660 2817
Size: 2660 157
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI ApaI ApaLI ApoI
AseI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BclI BglI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI EaeI EagI EcoICRI Eco57MI
EcoO109I FalI FspI HaeII Hin4I HincII HindIII KasI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PflMI PpuMI PshAI PspOMI PspXI PsrI
PvuI PvuII SacI SalI ScaI SexAI SfcI SfoI
SmaI SmlI SphI StyI TatI Tth111I XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AleI AlfI
AloI AlwNI AscI AsiSI BaeI BcgI BciVI BfrBI
BglII BlpI BmtI BsaAI BsaXI BsgI BsiWI BsmI
BspEI BsrGI BssHII BstZ17I CspCI DraI DraIII DrdI
EarI EciI EcoNI EcoRI EcoRV FseI FspAI HpaI
KpnI MfeI NcoI NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PsiI PstI
RsrII SacII SanDI SapI SbfI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TstI
XbaI ZraI