Restriction map for
pF1KSDA0385
(Linear) (Six-base) MAPSORT of: KIAA0385.seq Check: 9115 from: 1 to: 4110
KIAA0385.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AccI GT'mk_AC
Cuts at: 0 77 1697 3032 3076 3605 4110
Size: 77 1620 1335 44 529 505
Fragments arranged by size:
1620 1335 529 505 77 44
Acc65I G'GTAC_C
Cuts at: 0 369 4110
Size: 369 3741
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 1776 2776 4110
Size: 563 1213 1000 1334
Fragments arranged by size:
1334 1213 1000 563
AflIII A'CryG_T
Cuts at: 0 1477 1479 1536 4110
Size: 1477 2 57 2574
Fragments arranged by size:
2574 1477 57 2
AhdI GACnn_n'nnGTC
Cuts at: 0 1388 4110
Size: 1388 2722
AleI CACnn'nnGTG
Cuts at: 0 1480 2204 3703 3751 4110
Size: 1480 724 1499 48 359
Fragments arranged by size:
1499 1480 724 359 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1003 2146 4110
Size: 1003 1143 1964
Fragments arranged by size:
1964 1143 1003
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1816 1848 4110
Size: 1816 32 2262
Fragments arranged by size:
2262 1816 32
AlwNI CAG_nnn'CTG
Cuts at: 0 388 1643 1721 2093 2824 3325 4110
Size: 388 1255 78 372 731 501 785
Fragments arranged by size:
1255 785 731 501 388 372 78
ApaI G_GGCC'C
Cuts at: 0 138 2297 4110
Size: 138 2159 1813
Fragments arranged by size:
2159 1813 138
ApaLI G'TGCA_C
Cuts at: 0 932 4110
Size: 932 3178
ApoI r'AATT_y
Cuts at: 0 85 396 1315 2400 3901 4110
Size: 85 311 919 1085 1501 209
Fragments arranged by size:
1501 1085 919 311 209 85
AseI AT'TA_AT
Cuts at: 0 3020 4110
Size: 3020 1090
AvaI C'yCGr_G
Cuts at: 0 125 159 1041 2296 2921 3146 3560 3737
Size: 125 34 882 1255 625 225 414 177
Cuts at: 3737 4110
Size: 373
Fragments arranged by size:
1255 882 625 414 373 225 177 125
34
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1468 1501 4110
Size: 1468 33 2609
Fragments arranged by size:
2609 1468 33
BamHI G'GATC_C
Cuts at: 0 1203 4110
Size: 1203 2907
BanI G'GyrC_C
Cuts at: 0 369 916 2156 2661 2685 3742 4110
Size: 369 547 1240 505 24 1057 368
Fragments arranged by size:
1240 1057 547 505 369 368 24
BanII G_rGCy'C
Cuts at: 0 138 168 514 559 637 1406 1616 2297
Size: 138 30 346 45 78 769 210 681
Cuts at: 2297 3185 3332 4110
Size: 888 147 778
Fragments arranged by size:
888 778 769 681 346 210 147 138
78 45 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 1246 2233 2428 2903 4106 4110
Size: 1246 987 195 475 1203 4
Fragments arranged by size:
1246 1203 987 475 195 4
BbvCI CC'TCA_GC
Cuts at: 0 992 2825 3674 4110
Size: 992 1833 849 436
Fragments arranged by size:
1833 992 849 436
BciVI GTATCCnnnnn_n'
Cuts at: 0 2274 4110
Size: 2274 1836
BfrBI ATG'CAT
Cuts at: 0 2637 4110
Size: 2637 1473
BglI GCCn_nnn'nGGC
Cuts at: 0 846 4110
Size: 846 3264
BglII A'GATC_T
Cuts at: 0 1076 4110
Size: 1076 3034
BlpI GC'TnA_GC
Cuts at: 0 3318 3326 4110
Size: 3318 8 784
Fragments arranged by size:
3318 784 8
Bme1580I G_kGCm'C
Cuts at: 0 138 919 936 2297 2615 2696 3745 4110
Size: 138 781 17 1361 318 81 1049 365
Fragments arranged by size:
1361 1049 781 365 318 138 81 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 2 14 1188 1887 2602 3020 3342 3607
Size: 2 12 1174 699 715 418 322 265
Cuts at: 3607 3772 4090 4110
Size: 165 318 20
Fragments arranged by size:
1174 715 699 418 322 318 265 165
20 12 2
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 504 4110
Size: 504 3606
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 230 419 1139 1265 2209 4110
Size: 83 147 189 720 126 944 1901
Fragments arranged by size:
1901 944 720 189 147 126 83
Bpu10I CC'TnA_GC
Cuts at: 0 992 1400 1765 2825 3674 4110
Size: 992 408 365 1060 849 436
Fragments arranged by size:
1060 992 849 436 408 365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 864 4110
Size: 864 3246
BsaI GGTCTCn'nnnn_
Cuts at: 0 59 475 2185 2734 4110
Size: 59 416 1710 549 1376
Fragments arranged by size:
1710 1376 549 416 59
BsaAI yAC'GTr
Cuts at: 0 1480 4110
Size: 1480 2630
BsaBI GATnn'nnATC
Cuts at: 0 2677 4110
Size: 2677 1433
BsaHI Gr'CG_yC
Cuts at: 0 2528 4110
Size: 2528 1582
BsaWI w'CCGG_w
Cuts at: 0 3449 3932 4110
Size: 3449 483 178
Fragments arranged by size:
3449 483 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1608 1638 2536 2566 4110
Size: 1608 30 898 30 1544
Fragments arranged by size:
1608 1544 898 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 490 493 541 544 851 1396 2764 2842
Size: 490 3 48 3 307 545 1368 78
Cuts at: 2842 3526 3583 3586 3613 4110
Size: 684 57 3 27 497
Fragments arranged by size:
1368 684 545 497 490 307 78 57
48 27 3 3 3
BseYI C'CCAG_C
Cuts at: 0 237 243 388 1172 1714 2013 2469 3218
Size: 237 6 145 784 542 299 456 749
Cuts at: 3218 4110
Size: 892
Fragments arranged by size:
892 784 749 542 456 299 237 145
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 1125 1240 2900 3244 4110
Size: 632 493 115 1660 344 866
Fragments arranged by size:
1660 866 632 493 344 115
BsiHKAI G_wGCw'C
Cuts at: 0 936 1406 1904 2725 3332 3578 4110
Size: 936 470 498 821 607 246 532
Fragments arranged by size:
936 821 607 532 498 470 246
BsmI GAATG_Cn'
Cuts at: 0 776 1757 4110
Size: 776 981 2353
Fragments arranged by size:
2353 981 776
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2535 4110
Size: 2535 1575
Bsp1286I G_dGCh'C
Cuts at: 0 138 168 514 559 637 919 936 1406
Size: 138 30 346 45 78 282 17 470
Cuts at: 1406 1616 1904 2297 2615 2696 2725 3185 3332
Size: 210 288 393 318 81 29 460 147
Cuts at: 3332 3578 3745 4110
Size: 246 167 365
Fragments arranged by size:
470 460 393 365 346 318 288 282
246 210 167 147 138 81 78 45
30 29 17
BspEI T'CCGG_A
Cuts at: 0 3932 4110
Size: 3932 178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 182 1456 2062 2138 3965 4110
Size: 182 1274 606 76 1827 145
Fragments arranged by size:
1827 1274 606 182 145 76
BsrDI GCAATG_nn'
Cuts at: 0 936 1275 4110
Size: 936 339 2835
Fragments arranged by size:
2835 936 339
BsrFI r'CCGG_y
Cuts at: 0 1286 4110
Size: 1286 2824
BsrGI T'GTAC_A
Cuts at: 0 1988 4110
Size: 1988 2122
BssSI C'ACGA_G
Cuts at: 0 1258 1414 4110
Size: 1258 156 2696
Fragments arranged by size:
2696 1258 156
BstAPI GCAn_nnn'nTGC
Cuts at: 0 316 1232 1643 3435 4110
Size: 316 916 411 1792 675
Fragments arranged by size:
1792 916 675 411 316
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1243 1496 2206 2715 2781 2964 3753 4110
Size: 1243 253 710 509 66 183 789 357
Fragments arranged by size:
1243 789 710 509 357 253 183 66
BstYI r'GATC_y
Cuts at: 0 96 1076 1203 2213 2411 2765 4110
Size: 96 980 127 1010 198 354 1345
Fragments arranged by size:
1345 1010 980 354 198 127 96
Bsu36I CC'TnA_GG
Cuts at: 0 296 362 410 752 1748 1805 3065 4110
Size: 296 66 48 342 996 57 1260 1045
Fragments arranged by size:
1260 1045 996 342 296 66 57 48
BtgI C'CryG_G
Cuts at: 0 288 806 2939 4013 4110
Size: 288 518 2133 1074 97
Fragments arranged by size:
2133 1074 518 288 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3963 4110
Size: 3963 147
BtsI GCAGTG_nn'
Cuts at: 0 451 917 949 2112 2242 2438 4110
Size: 451 466 32 1163 130 196 1672
Fragments arranged by size:
1672 1163 466 451 196 130 32
DrdI GACnn_nn'nnGTC
Cuts at: 0 211 1693 4110
Size: 211 1482 2417
Fragments arranged by size:
2417 1482 211
EaeI y'GGCC_r
Cuts at: 0 2936 2954 4110
Size: 2936 18 1156
Fragments arranged by size:
2936 1156 18
EarI CTCTTCn'nnn_
Cuts at: 0 467 706 1003 1414 1795 2567 3817 4110
Size: 467 239 297 411 381 772 1250 293
Fragments arranged by size:
1250 772 467 411 381 297 293 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3772 4110
Size: 3772 338
EcoICRI GAG'CTC
Cuts at: 0 1404 3330 4110
Size: 1404 1926 780
Fragments arranged by size:
1926 1404 780
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 230 419 563 1139 1265 1776 2209
Size: 83 147 189 144 576 126 511 433
Cuts at: 2209 2776 4110
Size: 567 1334
Fragments arranged by size:
1334 576 567 511 433 189 147 144
126 83
EcoNI CCTnn'n_nnAGG
Cuts at: 0 344 380 1165 1258 4110
Size: 344 36 785 93 2852
Fragments arranged by size:
2852 785 344 93 36
EcoO109I rG'GnC_Cy
Cuts at: 0 135 1251 2381 3061 3808 4110
Size: 135 1116 1130 680 747 302
Fragments arranged by size:
1130 1116 747 680 302 135
EcoRI G'AATT_C
Cuts at: 0 3901 4110
Size: 3901 209
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1578 2034 4110
Size: 1578 456 2076
Fragments arranged by size:
2076 1578 456
HaeII r_GCGC'y
Cuts at: 0 858 906 1185 2175 4110
Size: 858 48 279 990 1935
Fragments arranged by size:
1935 990 858 279 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 957 989 1374 1406 1516 1548 2331 2363
Size: 957 32 385 32 110 32 783 32
Cuts at: 2363 2505 2536 2537 2568 2827 2859 2915 2947
Size: 142 31 1 31 259 32 56 32
Cuts at: 2947 3058 3090 3096 3128 4110
Size: 111 32 6 32 982
Fragments arranged by size:
982 957 783 385 259 142 111 110
56 32 32 32 32 32 32 32
32 31 31 6 1
HincII GTy'rAC
Cuts at: 0 1686 4110
Size: 1686 2424
HindIII A'AGCT_T
Cuts at: 0 1959 4110
Size: 1959 2151
KpnI G_GTAC'C
Cuts at: 0 373 4110
Size: 373 3737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2798 4010 4110
Size: 2798 1212 100
Fragments arranged by size:
2798 1212 100
MscI TGG'CCA
Cuts at: 0 2938 2956 4110
Size: 2938 18 1154
Fragments arranged by size:
2938 1154 18
MslI CAynn'nnrTG
Cuts at: 0 781 922 1480 2204 2608 2620 3634 3703
Size: 781 141 558 724 404 12 1014 69
Cuts at: 3703 3751 4110
Size: 48 359
Fragments arranged by size:
1014 781 724 558 404 359 141 69
48 12
MspA1I CmG'CkG
Cuts at: 0 1718 1946 3322 3673 3881 4110
Size: 1718 228 1376 351 208 229
Fragments arranged by size:
1718 1376 351 229 228 208
NcoI C'CATG_G
Cuts at: 0 806 2939 4013 4110
Size: 806 2133 1074 97
Fragments arranged by size:
2133 1074 806 97
NdeI CA'TA_TG
Cuts at: 0 1231 3195 4110
Size: 1231 1964 915
Fragments arranged by size:
1964 1231 915
NsiI A_TGCA'T
Cuts at: 0 2639 4110
Size: 2639 1471
NspI r_CATG'y
Cuts at: 0 870 1540 3687 4029 4041 4110
Size: 870 670 2147 342 12 69
Fragments arranged by size:
2147 870 670 342 69 12
PasI CC'CwG_GG
Cuts at: 0 517 4110
Size: 517 3593
PciI A'CATG_T
Cuts at: 0 1536 4110
Size: 1536 2574
PflMI CCAn_nnn'nTGG
Cuts at: 0 388 670 1721 2201 4110
Size: 388 282 1051 480 1909
Fragments arranged by size:
1909 1051 480 388 282
PfoI T'CCnGG_A
Cuts at: 0 215 2891 3810 4110
Size: 215 2676 919 300
Fragments arranged by size:
2676 919 300 215
PmlI CAC'GTG
Cuts at: 0 1480 4110
Size: 1480 2630
PpuMI rG'GwC_Cy
Cuts at: 0 1251 2381 3808 4110
Size: 1251 1130 1427 302
Fragments arranged by size:
1427 1251 1130 302
PshAI GACnn'nnGTC
Cuts at: 0 290 3806 4110
Size: 290 3516 304
Fragments arranged by size:
3516 304 290
PspOMI G'GGCC_C
Cuts at: 0 134 2293 4110
Size: 134 2159 1817
Fragments arranged by size:
2159 1817 134
PspXI vC'TCGA_Gb
Cuts at: 0 125 4110
Size: 125 3985
PstI C_TGCA'G
Cuts at: 0 458 912 956 1228 1297 1639 1711 1969
Size: 458 454 44 272 69 342 72 258
Cuts at: 1969 2107 3668 4110
Size: 138 1561 442
Fragments arranged by size:
1561 458 454 442 342 272 258 138
72 69 44
PvuII CAG'CTG
Cuts at: 0 1718 3322 3673 4110
Size: 1718 1604 351 437
Fragments arranged by size:
1718 1604 437 351
SacI G_AGCT'C
Cuts at: 0 1406 3332 4110
Size: 1406 1926 778
Fragments arranged by size:
1926 1406 778
SapI GCTCTTCn'nnn_
Cuts at: 0 1003 4110
Size: 1003 3107
ScaI AGT'ACT
Cuts at: 0 3433 4110
Size: 3433 677
SfcI C'TryA_G
Cuts at: 0 454 908 952 1224 1293 1635 1707 1965
Size: 454 454 44 272 69 342 72 258
Cuts at: 1965 2103 2253 3664 4110
Size: 138 150 1411 446
Fragments arranged by size:
1411 454 454 446 342 272 258 150
138 72 69 44
SmaI CCC'GGG
Cuts at: 0 3148 4110
Size: 3148 962
SmlI C'TyrA_G
Cuts at: 0 125 879 2921 3560 4110
Size: 125 754 2042 639 550
Fragments arranged by size:
2042 754 639 550 125
SphI G_CATG'C
Cuts at: 0 4041 4110
Size: 4041 69
StuI AGG'CCT
Cuts at: 0 2842 4110
Size: 2842 1268
StyI C'CwwG_G
Cuts at: 0 540 806 1088 2714 2780 2939 3749 4013
Size: 540 266 282 1626 66 159 810 264
Cuts at: 4013 4110
Size: 97
Fragments arranged by size:
1626 810 540 282 266 264 159 97
66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3210 4110
Size: 3210 900
TatI w'GTAC_w
Cuts at: 0 1988 2035 2642 3431 4110
Size: 1988 47 607 789 679
Fragments arranged by size:
1988 789 679 607 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1608 1640 4110
Size: 1608 32 2470
Fragments arranged by size:
2470 1608 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 128 234 4110
Size: 128 106 3876
Fragments arranged by size:
3876 128 106
XhoI C'TCGA_G
Cuts at: 0 125 2921 3560 4110
Size: 125 2796 639 550
Fragments arranged by size:
2796 639 550 125
XmaI C'CCGG_G
Cuts at: 0 3146 4110
Size: 3146 964
XmnI GAAnn'nnTTC
Cuts at: 0 1960 3463 4110
Size: 1960 1503 647
Fragments arranged by size:
1960 1503 647
Enzymes that do cut:
AccI Acc65I AcuI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AvaI BaeI BamHI
BanI BanII BbsI BbvCI BciVI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII KpnI
MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI
PasI PciI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PstI PvuII SacI SapI ScaI SfcI SmaI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AscI AsiSI
AvrII BbeI BcgI BclI BmgBI BmtI BsiEI BsiWI
BspHI BsrBI BssHII BstBI BstEII BstZ17I ClaI CspCI
DraI DraIII EagI EcoRV FseI FspI FspAI HpaI
KasI MfeI MluI NaeI NarI NgoMIV NheI NotI
NruI PacI PmeI PpiI PsiI PsrI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
ZraI