Restriction map for
pF1KSDA0387
(Linear) (Six-base) MAPSORT of: KIAA0387.seq Check: 8598 from: 1 to: 3114
KIAA0387.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 539 1671 3114
Size: 539 1132 1443
Fragments arranged by size:
1443 1132 539
AatII G_ACGT'C
Cuts at: 0 1667 3114
Size: 1667 1447
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 1021 1361 2029 2305 2455 2764 3114
Size: 449 572 340 668 276 150 309 350
Fragments arranged by size:
668 572 449 350 340 309 276 150
AfeI AGC'GCT
Cuts at: 0 2321 3114
Size: 2321 793
AflIII A'CryG_T
Cuts at: 0 662 2126 3114
Size: 662 1464 988
Fragments arranged by size:
1464 988 662
AhdI GACnn_n'nnGTC
Cuts at: 0 1287 3114
Size: 1287 1827
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2680 2712 3114
Size: 2680 32 402
Fragments arranged by size:
2680 402 32
AlwNI CAG_nnn'CTG
Cuts at: 0 162 312 2073 3114
Size: 162 150 1761 1041
Fragments arranged by size:
1761 1041 162 150
ApaI G_GGCC'C
Cuts at: 0 692 1508 2122 3114
Size: 692 816 614 992
Fragments arranged by size:
992 816 692 614
ApaLI G'TGCA_C
Cuts at: 0 878 3114
Size: 878 2236
ApoI r'AATT_y
Cuts at: 0 1832 3114
Size: 1832 1282
AvaI C'yCGr_G
Cuts at: 0 190 685 841 1112 1264 1462 1636 2022
Size: 190 495 156 271 152 198 174 386
Cuts at: 2022 2423 2499 3114
Size: 401 76 615
Fragments arranged by size:
615 495 401 386 271 198 190 174
156 152 76
BanI G'GyrC_C
Cuts at: 0 248 909 1683 2926 3114
Size: 248 661 774 1243 188
Fragments arranged by size:
1243 774 661 248 188
BanII G_rGCy'C
Cuts at: 0 692 755 1311 1463 1487 1508 2122 2345
Size: 692 63 556 152 24 21 614 223
Cuts at: 2345 3114
Size: 769
Fragments arranged by size:
769 692 614 556 223 152 63 24
21
BbsI GAAGACnn'nnnn_
Cuts at: 0 962 1358 1367 1904 3114
Size: 962 396 9 537 1210
Fragments arranged by size:
1210 962 537 396 9
BbvCI CC'TCA_GC
Cuts at: 0 614 1025 1552 3077 3114
Size: 614 411 527 1525 37
Fragments arranged by size:
1525 614 527 411 37
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1680 1714 2026 2060 3114
Size: 1680 34 312 34 1054
Fragments arranged by size:
1680 1054 312 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1043 2335 2548 2924 3114
Size: 1043 1292 213 376 190
Fragments arranged by size:
1292 1043 376 213 190
BglII A'GATC_T
Cuts at: 0 1431 3114
Size: 1431 1683
BlpI GC'TnA_GC
Cuts at: 0 754 1067 3114
Size: 754 313 2047
Fragments arranged by size:
2047 754 313
Bme1580I G_kGCm'C
Cuts at: 0 692 882 1508 1688 2122 2384 3114
Size: 692 190 626 180 434 262 730
Fragments arranged by size:
730 692 626 434 262 190 180
BmgBI CAC'GTC
Cuts at: 0 573 665 1707 2129 3114
Size: 573 92 1042 422 985
Fragments arranged by size:
1042 985 573 422 92
BmrI ACTGGGnnnn_n'
Cuts at: 0 2148 3114
Size: 2148 966
BmtI G_CTAG'C
Cuts at: 0 2480 3114
Size: 2480 634
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1836 2037 3114
Size: 1836 201 1077
Fragments arranged by size:
1836 1077 201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 724 877 1069 1169 1325 1831 2323
Size: 562 162 153 192 100 156 506 492
Cuts at: 2323 3022 3114
Size: 699 92
Fragments arranged by size:
699 562 506 492 192 162 156 153
100 92
Bpu10I CC'TnA_GC
Cuts at: 0 614 1025 1541 1552 3077 3114
Size: 614 411 516 11 1525 37
Fragments arranged by size:
1525 614 516 411 37 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 870 1852 2811 3030 3082 3114
Size: 870 982 959 219 52 32
Fragments arranged by size:
982 959 870 219 52 32
BsaI GGTCTCn'nnnn_
Cuts at: 0 1295 1616 1935 2700 3015 3114
Size: 1295 321 319 765 315 99
Fragments arranged by size:
1295 765 321 319 315 99
BsaAI yAC'GTr
Cuts at: 0 353 3114
Size: 353 2761
BsaBI GATnn'nnATC
Cuts at: 0 2986 3114
Size: 2986 128
BsaHI Gr'CG_yC
Cuts at: 0 423 525 1664 1952 2624 3114
Size: 423 102 1139 288 672 490
Fragments arranged by size:
1139 672 490 423 288 102
BsaWI w'CCGG_w
Cuts at: 0 1843 3114
Size: 1843 1271
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 471 501 535 565 616 646 666 696
Size: 471 30 34 30 51 30 20 30
Cuts at: 696 850 880 2296 2326 2680 2710 3114
Size: 154 30 1416 30 354 30 404
Fragments arranged by size:
1416 471 404 354 154 51 34 30
30 30 30 30 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 208 492 1399 1531 1871 2230 2386 2745
Size: 208 284 907 132 340 359 156 359
Cuts at: 2745 2814 2825 3094 3114
Size: 69 11 269 20
Fragments arranged by size:
907 359 359 340 284 269 208 156
132 69 20 11
BseYI C'CCAG_C
Cuts at: 0 441 561 698 831 1437 1687 2176 3114
Size: 441 120 137 133 606 250 489 938
Fragments arranged by size:
938 606 489 441 250 137 133 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1231 2066 2245 2933 3114
Size: 187 1044 835 179 688 181
Fragments arranged by size:
1044 835 688 187 181 179
BsiHKAI G_wGCw'C
Cuts at: 0 578 755 882 984 1358 1487 1542 2705
Size: 578 177 127 102 374 129 55 1163
Cuts at: 2705 3008 3114
Size: 303 106
Fragments arranged by size:
1163 578 374 303 177 129 127 106
102 55
BsmI GAATG_Cn'
Cuts at: 0 896 1452 3114
Size: 896 556 1662
Fragments arranged by size:
1662 896 556
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2151 2758 3114
Size: 2151 607 356
Fragments arranged by size:
2151 607 356
Bsp1286I G_dGCh'C
Cuts at: 0 578 692 755 882 984 1311 1358 1463
Size: 578 114 63 127 102 327 47 105
Cuts at: 1463 1487 1508 1542 1688 2122 2345 2384 2705
Size: 24 21 34 146 434 223 39 321
Cuts at: 2705 3008 3114
Size: 303 106
Fragments arranged by size:
578 434 327 321 303 223 146 127
114 106 105 102 63 47 39 34
24 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 539 1016 1671 2759 3114
Size: 539 477 655 1088 355
Fragments arranged by size:
1088 655 539 477 355
BsrBI CCG'CTC
Cuts at: 0 1132 2393 2579 2924 3114
Size: 1132 1261 186 345 190
Fragments arranged by size:
1261 1132 345 190 186
BsrDI GCAATG_nn'
Cuts at: 0 272 2898 3114
Size: 272 2626 216
Fragments arranged by size:
2626 272 216
BsrFI r'CCGG_y
Cuts at: 0 1020 2248 2297 3114
Size: 1020 1228 49 817
Fragments arranged by size:
1228 1020 817 49
BssSI C'ACGA_G
Cuts at: 0 706 3114
Size: 706 2408
BstAPI GCAn_nnn'nTGC
Cuts at: 0 312 3114
Size: 312 2802
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1501 3114
Size: 1501 1613
BstYI r'GATC_y
Cuts at: 0 1431 3114
Size: 1431 1683
Bsu36I CC'TnA_GG
Cuts at: 0 418 761 1883 2114 3114
Size: 418 343 1122 231 1000
Fragments arranged by size:
1122 1000 418 343 231
BtgI C'CryG_G
Cuts at: 0 296 721 1101 1182 1284 2546 3071 3114
Size: 296 425 380 81 102 1262 525 43
Fragments arranged by size:
1262 525 425 380 296 102 81 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 989 1899 2502 3114
Size: 989 910 603 612
Fragments arranged by size:
989 910 612 603
BtsI GCAGTG_nn'
Cuts at: 0 22 163 1733 2054 2638 2910 3114
Size: 22 141 1570 321 584 272 204
Fragments arranged by size:
1570 584 321 272 204 141 22
DrdI GACnn_nn'nnGTC
Cuts at: 0 2812 3114
Size: 2812 302
EaeI y'GGCC_r
Cuts at: 0 509 732 2090 2098 2250 2647 2963 3114
Size: 509 223 1358 8 152 397 316 151
Fragments arranged by size:
1358 509 397 316 223 152 151 8
EarI CTCTTCn'nnn_
Cuts at: 0 1376 1586 3114
Size: 1376 210 1528
Fragments arranged by size:
1528 1376 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1174 1903 1975 2353 2455 3114
Size: 1174 729 72 378 102 659
Fragments arranged by size:
1174 729 659 378 102 72
EcoICRI GAG'CTC
Cuts at: 0 753 1485 3114
Size: 753 732 1629
Fragments arranged by size:
1629 753 732
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 562 724 877 1021 1069 1169 1325
Size: 449 113 162 153 144 48 100 156
Cuts at: 1325 1361 1831 2029 2305 2323 2455 2764 3022
Size: 36 470 198 276 18 132 309 258
Cuts at: 3022 3114
Size: 92
Fragments arranged by size:
470 449 309 276 258 198 162 156
153 144 132 113 100 92 48 36
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 84 190 417 553 1000 1276 1320 1678
Size: 84 106 227 136 447 276 44 358
Cuts at: 1678 2434 2826 3114
Size: 756 392 288
Fragments arranged by size:
756 447 392 358 288 276 227 136
106 84 44
EcoO109I rG'GnC_Cy
Cuts at: 0 198 449 546 558 584 689 711 827
Size: 198 251 97 12 26 105 22 116
Cuts at: 827 1005 1035 1269 1325 1505 1548 2118 2427
Size: 178 30 234 56 180 43 570 309
Cuts at: 2427 2831 3102 3114
Size: 404 271 12
Fragments arranged by size:
570 404 309 271 251 234 198 180
178 116 105 97 56 43 30 26
22 12 12
EcoRV GAT'ATC
Cuts at: 0 2988 3114
Size: 2988 126
HaeII r_GCGC'y
Cuts at: 0 312 1748 2201 2323 3114
Size: 312 1436 453 122 791
Fragments arranged by size:
1436 791 453 312 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 471 503 535 567 616 648 666 698
Size: 471 32 32 32 49 32 18 32
Cuts at: 698 1273 1305 2644 2676 2724 2756 3114
Size: 575 32 1339 32 48 32 358
Fragments arranged by size:
1339 575 471 358 49 48 32 32
32 32 32 32 32 32 18
HincII GTy'rAC
Cuts at: 0 1794 3114
Size: 1794 1320
HindIII A'AGCT_T
Cuts at: 0 331 3114
Size: 331 2783
MfeI C'AATT_G
Cuts at: 0 1567 3114
Size: 1567 1547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 546 2255 3114
Size: 546 1709 859
Fragments arranged by size:
1709 859 546
MscI TGG'CCA
Cuts at: 0 511 2092 2965 3114
Size: 511 1581 873 149
Fragments arranged by size:
1581 873 511 149
MslI CAynn'nnrTG
Cuts at: 0 2678 2903 3114
Size: 2678 225 211
Fragments arranged by size:
2678 225 211
MspA1I CmG'CkG
Cuts at: 0 613 1471 1534 2535 3114
Size: 613 858 63 1001 579
Fragments arranged by size:
1001 858 613 579 63
NaeI GCC'GGC
Cuts at: 0 1022 3114
Size: 1022 2092
NcoI C'CATG_G
Cuts at: 0 1101 3114
Size: 1101 2013
NgoMIV G'CCGG_C
Cuts at: 0 1020 3114
Size: 1020 2094
NheI G'CTAG_C
Cuts at: 0 2476 3114
Size: 2476 638
PasI CC'CwG_GG
Cuts at: 0 86 1123 1137 2526 3114
Size: 86 1037 14 1389 588
Fragments arranged by size:
1389 1037 588 86 14
PflMI CCAn_nnn'nTGG
Cuts at: 0 795 2088 3114
Size: 795 1293 1026
Fragments arranged by size:
1293 1026 795
PfoI T'CCnGG_A
Cuts at: 0 539 2837 3114
Size: 539 2298 277
Fragments arranged by size:
2298 539 277
PmlI CAC'GTG
Cuts at: 0 353 3114
Size: 353 2761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2680 2712 3114
Size: 2680 32 402
Fragments arranged by size:
2680 402 32
PpuMI rG'GwC_Cy
Cuts at: 0 584 711 1005 2427 2831 3114
Size: 584 127 294 1422 404 283
Fragments arranged by size:
1422 584 404 294 283 127
PspOMI G'GGCC_C
Cuts at: 0 688 1504 2118 3114
Size: 688 816 614 996
Fragments arranged by size:
996 816 688 614
PspXI vC'TCGA_Gb
Cuts at: 0 190 1112 3114
Size: 190 922 2002
Fragments arranged by size:
2002 922 190
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2497 2529 3114
Size: 2497 32 585
Fragments arranged by size:
2497 585 32
PstI C_TGCA'G
Cuts at: 0 308 1013 1280 1682 2756 3114
Size: 308 705 267 402 1074 358
Fragments arranged by size:
1074 705 402 358 308 267
SacI G_AGCT'C
Cuts at: 0 755 1487 3114
Size: 755 732 1627
Fragments arranged by size:
1627 755 732
SbfI CC_TGCA'GG
Cuts at: 0 1280 1682 3114
Size: 1280 402 1432
Fragments arranged by size:
1432 1280 402
SexAI A'CCwGG_T
Cuts at: 0 2690 3114
Size: 2690 424
SfcI C'TryA_G
Cuts at: 0 304 1009 1276 1678 2752 2868 3114
Size: 304 705 267 402 1074 116 246
Fragments arranged by size:
1074 705 402 304 267 246 116
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1043 3114
Size: 1043 2071
SmaI CCC'GGG
Cuts at: 0 687 843 1464 2425 3114
Size: 687 156 621 961 689
Fragments arranged by size:
961 689 687 621 156
SmlI C'TyrA_G
Cuts at: 0 190 885 1112 1867 2826 3009 3097 3114
Size: 190 695 227 755 959 183 88 17
Fragments arranged by size:
959 755 695 227 190 183 88 17
SspI AAT'ATT
Cuts at: 0 19 3114
Size: 19 3095
StuI AGG'CCT
Cuts at: 0 220 900 3114
Size: 220 680 2214
Fragments arranged by size:
2214 680 220
StyI C'CwwG_G
Cuts at: 0 1101 1169 3114
Size: 1101 68 1945
Fragments arranged by size:
1945 1101 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2123 2539 2822 3114
Size: 2123 416 283 292
Fragments arranged by size:
2123 416 292 283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 136 168 1712 1744 2296 2308 2328 2340
Size: 136 32 1544 32 552 12 20 12
Cuts at: 2340 3114
Size: 774
Fragments arranged by size:
1544 774 552 136 32 32 20 12
12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 903 1434 1773 3114
Size: 903 531 339 1341
Fragments arranged by size:
1341 903 531 339
XhoI C'TCGA_G
Cuts at: 0 190 1112 3114
Size: 190 922 2002
Fragments arranged by size:
2002 922 190
XmaI C'CCGG_G
Cuts at: 0 685 841 1462 2423 3114
Size: 685 156 621 961 691
Fragments arranged by size:
961 691 685 621 156
XmnI GAAnn'nnTTC
Cuts at: 0 61 3114
Size: 61 3053
ZraI GAC'GTC
Cuts at: 0 1665 3114
Size: 1665 1449
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI AloI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbsI BbvCI
BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I
HincII HindIII MfeI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NheI PasI PflMI PfoI PmlI PpiI
PpuMI PspOMI PspXI PsrI PstI SacI SbfI SexAI
SfcI SfiI SmaI SmlI SspI StuI StyI TaqII
TstI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AleI AlfI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI
BfrBI BsiEI BsiWI BspEI BspHI BsrGI BssHII BstBI
BstEII BstZ17I ClaI CspCI DraI DraIII EagI EcoRI
FalI FseI FspI FspAI HpaI KasI KpnI MluI
NarI NdeI NotI NruI NsiI NspI PacI PciI
PmeI PshAI PsiI PvuI PvuII RsrII SacII SalI
SanDI SapI ScaI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SwaI TatI Tth111I XbaI