Restriction map for
pF1KA0394
(Linear) (Six-base) MAPSORT of: KIAA0394.seq Check: 5421 from: 1 to: 1236
KIAA0394.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 1236
Size: 511 725
AflIII A'CryG_T
Cuts at: 0 1109 1236
Size: 1109 127
AgeI A'CCGG_T
Cuts at: 0 265 1236
Size: 265 971
AleI CACnn'nnGTG
Cuts at: 0 248 1236
Size: 248 988
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 520 1236
Size: 520 716
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 271 303 461 493 1236
Size: 271 32 158 32 743
Fragments arranged by size:
743 271 158 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 238 282 381 384 876 1074 1236
Size: 238 44 99 3 492 198 162
Fragments arranged by size:
492 238 198 162 99 44 3
ApaI G_GGCC'C
Cuts at: 0 636 1236
Size: 636 600
ApoI r'AATT_y
Cuts at: 0 523 1236
Size: 523 713
BamHI G'GATC_C
Cuts at: 0 286 1236
Size: 286 950
BanII G_rGCy'C
Cuts at: 0 178 636 1136 1236
Size: 178 458 500 100
Fragments arranged by size:
500 458 178 100
BbsI GAAGACnn'nnnn_
Cuts at: 0 560 871 1236
Size: 560 311 365
Fragments arranged by size:
560 365 311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 774 808 1236
Size: 774 34 428
Fragments arranged by size:
774 428 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 193 1236
Size: 193 1043
BlpI GC'TnA_GC
Cuts at: 0 575 889 1236
Size: 575 314 347
Fragments arranged by size:
575 347 314
Bme1580I G_kGCm'C
Cuts at: 0 636 1236
Size: 636 600
BmrI ACTGGGnnnn_n'
Cuts at: 0 981 1236
Size: 981 255
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 630 970 1236
Size: 630 340 266
Fragments arranged by size:
630 340 266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 463 877 898 965 1060 1236
Size: 215 248 414 21 67 95 176
Fragments arranged by size:
414 248 215 176 95 67 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 1236
Size: 661 575
BsaI GGTCTCn'nnnn_
Cuts at: 0 106 845 1236
Size: 106 739 391
Fragments arranged by size:
739 391 106
BsaBI GATnn'nnATC
Cuts at: 0 291 1236
Size: 291 945
BsaWI w'CCGG_w
Cuts at: 0 265 1236
Size: 265 971
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 461 491 1236
Size: 461 30 745
Fragments arranged by size:
745 461 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 919 1236
Size: 919 317
BseYI C'CCAG_C
Cuts at: 0 130 219 460 869 1236
Size: 130 89 241 409 367
Fragments arranged by size:
409 367 241 130 89
BsiHKAI G_wGCw'C
Cuts at: 0 178 1199 1236
Size: 178 1021 37
Fragments arranged by size:
1021 178 37
BsmBI CGTCTCn'nnnn_
Cuts at: 0 941 1236
Size: 941 295
Bsp1286I G_dGCh'C
Cuts at: 0 178 636 1136 1199 1236
Size: 178 458 500 63 37
Fragments arranged by size:
500 458 178 63 37
BsrBI CCG'CTC
Cuts at: 0 843 1035 1236
Size: 843 192 201
Fragments arranged by size:
843 201 192
BsrDI GCAATG_nn'
Cuts at: 0 770 1236
Size: 770 466
BsrFI r'CCGG_y
Cuts at: 0 80 265 1236
Size: 80 185 971
Fragments arranged by size:
971 185 80
BstBI TT'CG_AA
Cuts at: 0 1152 1236
Size: 1152 84
BstEII G'GTnAC_C
Cuts at: 0 1011 1236
Size: 1011 225
BstXI CCAn_nnnn'nTGG
Cuts at: 0 53 398 1023 1236
Size: 53 345 625 213
Fragments arranged by size:
625 345 213 53
BstYI r'GATC_y
Cuts at: 0 286 969 1236
Size: 286 683 267
Fragments arranged by size:
683 286 267
Bsu36I CC'TnA_GG
Cuts at: 0 74 1236
Size: 74 1162
BtgI C'CryG_G
Cuts at: 0 1136 1236
Size: 1136 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 278 1236
Size: 278 958
EaeI y'GGCC_r
Cuts at: 0 1166 1236
Size: 1166 70
EarI CTCTTCn'nnn_
Cuts at: 0 640 998 1236
Size: 640 358 238
Fragments arranged by size:
640 358 238
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2 670 1204 1236
Size: 2 668 534 32
Fragments arranged by size:
668 534 32 2
EcoICRI GAG'CTC
Cuts at: 0 176 1236
Size: 176 1060
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 463 511 877 898 965 1060 1236
Size: 215 248 48 366 21 67 95 176
Fragments arranged by size:
366 248 215 176 95 67 48 21
EcoO109I rG'GnC_Cy
Cuts at: 0 215 435 1236
Size: 215 220 801
Fragments arranged by size:
801 220 215
EcoRI G'AATT_C
Cuts at: 0 523 1236
Size: 523 713
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 336 368 1236
Size: 336 32 868
Fragments arranged by size:
868 336 32
HincII GTy'rAC
Cuts at: 0 96 1236
Size: 96 1140
HindIII A'AGCT_T
Cuts at: 0 691 1236
Size: 691 545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 1236
Size: 211 1025
MslI CAynn'nnrTG
Cuts at: 0 248 1236
Size: 248 988
MspA1I CmG'CkG
Cuts at: 0 381 489 876 1092 1146 1236
Size: 381 108 387 216 54 90
Fragments arranged by size:
387 381 216 108 90 54
NaeI GCC'GGC
Cuts at: 0 82 1236
Size: 82 1154
NgoMIV G'CCGG_C
Cuts at: 0 80 1236
Size: 80 1156
NspI r_CATG'y
Cuts at: 0 1113 1236
Size: 1113 123
PciI A'CATG_T
Cuts at: 0 1109 1236
Size: 1109 127
PflMI CCAn_nnn'nTGG
Cuts at: 0 282 876 1236
Size: 282 594 360
Fragments arranged by size:
594 360 282
PfoI T'CCnGG_A
Cuts at: 0 530 1236
Size: 530 706
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 461 493 1236
Size: 461 32 743
Fragments arranged by size:
743 461 32
PpuMI rG'GwC_Cy
Cuts at: 0 215 435 1236
Size: 215 220 801
Fragments arranged by size:
801 220 215
PspOMI G'GGCC_C
Cuts at: 0 632 1236
Size: 632 604
PvuII CAG'CTG
Cuts at: 0 381 489 876 1092 1146 1236
Size: 381 108 387 216 54 90
Fragments arranged by size:
387 381 216 108 90 54
SacI G_AGCT'C
Cuts at: 0 178 1236
Size: 178 1058
SanDI GG'GwC_CC
Cuts at: 0 215 1236
Size: 215 1021
SapI GCTCTTCn'nnn_
Cuts at: 0 640 1236
Size: 640 596
SgrAI Cr'CCGG_yG
Cuts at: 0 265 1236
Size: 265 971
SmlI C'TyrA_G
Cuts at: 0 676 1236
Size: 676 560
StyI C'CwwG_G
Cuts at: 0 441 593 617 1236
Size: 441 152 24 619
Fragments arranged by size:
619 441 152 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 371 1236
Size: 371 865
TatI w'GTAC_w
Cuts at: 0 1082 1236
Size: 1082 154
Enzymes that do cut:
AcuI AflIII AgeI AleI AlfI AloI AlwNI ApaI
ApoI BamHI BanII BbsI BcgI BciVI BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaBI BsaWI BsaXI
BseRI BseYI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI Hin4I HincII
HindIII MmeI MslI MspA1I NaeI NgoMIV NspI PciI
PflMI PfoI PpiI PpuMI PspOMI PvuII SacI SanDI
SapI SgrAI SmlI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI
BbeI BbvCI BclI BfrBI BglI BglII BmgBI BmtI
Bpu10I BsaAI BsaHI BsgI BsiEI BsiWI BsmI BspEI
BspHI BspMI BsrGI BssHII BssSI BstAPI BstZ17I BtsI
ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRV
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI MscI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PmeI PmlI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI