Restriction map for
pF1KA0397
(Linear) (Six-base) MAPSORT of: KIAA0397.seq Check: 3893 from: 1 to: 3018
KIAA0397.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1085 1770 1803 3018
Size: 1085 685 33 1215
Fragments arranged by size:
1215 1085 685 33
AatII G_ACGT'C
Cuts at: 0 1724 2465 3018
Size: 1724 741 553
Fragments arranged by size:
1724 741 553
Acc65I G'GTAC_C
Cuts at: 0 1805 3018
Size: 1805 1213
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 948 2033 2562 3018
Size: 892 56 1085 529 456
Fragments arranged by size:
1085 892 529 456 56
AflIII A'CryG_T
Cuts at: 0 384 1622 2477 3018
Size: 384 1238 855 541
Fragments arranged by size:
1238 855 541 384
AgeI A'CCGG_T
Cuts at: 0 1439 3018
Size: 1439 1579
AleI CACnn'nnGTG
Cuts at: 0 220 1015 2068 3018
Size: 220 795 1053 950
Fragments arranged by size:
1053 950 795 220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 869 1033 2966 3018
Size: 869 164 1933 52
Fragments arranged by size:
1933 869 164 52
AlwNI CAG_nnn'CTG
Cuts at: 0 273 493 1421 2670 3018
Size: 273 220 928 1249 348
Fragments arranged by size:
1249 928 348 273 220
ApaI G_GGCC'C
Cuts at: 0 1325 2234 3018
Size: 1325 909 784
Fragments arranged by size:
1325 909 784
ApaLI G'TGCA_C
Cuts at: 0 1561 3018
Size: 1561 1457
AvaI C'yCGr_G
Cuts at: 0 1118 1490 1593 2050 2148 2446 2975 3018
Size: 1118 372 103 457 98 298 529 43
Fragments arranged by size:
1118 529 457 372 298 103 98 43
BamHI G'GATC_C
Cuts at: 0 671 2268 3018
Size: 671 1597 750
Fragments arranged by size:
1597 750 671
BanI G'GyrC_C
Cuts at: 0 611 957 1340 1805 2062 2529 2623 3018
Size: 611 346 383 465 257 467 94 395
Fragments arranged by size:
611 467 465 395 383 346 257 94
BanII G_rGCy'C
Cuts at: 0 340 1136 1325 1352 1369 2003 2234 2264
Size: 340 796 189 27 17 634 231 30
Cuts at: 2264 3018
Size: 754
Fragments arranged by size:
796 754 634 340 231 189 30 27
17
BbeI G_GCGC'C
Cuts at: 0 2533 3018
Size: 2533 485
BbsI GAAGACnn'nnnn_
Cuts at: 0 110 235 3018
Size: 110 125 2783
Fragments arranged by size:
2783 125 110
BbvCI CC'TCA_GC
Cuts at: 0 364 3018
Size: 364 2654
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1893 1927 3018
Size: 1893 34 1091
Fragments arranged by size:
1893 1091 34
BclI T'GATC_A
Cuts at: 0 2556 3018
Size: 2556 462
BfrBI ATG'CAT
Cuts at: 0 2697 3018
Size: 2697 321
BglI GCCn_nnn'nGGC
Cuts at: 0 1034 1338 1452 3018
Size: 1034 304 114 1566
Fragments arranged by size:
1566 1034 304 114
BlpI GC'TnA_GC
Cuts at: 0 339 2939 3018
Size: 339 2600 79
Fragments arranged by size:
2600 339 79
Bme1580I G_kGCm'C
Cuts at: 0 238 729 962 1228 1325 1565 2065 2234
Size: 238 491 233 266 97 240 500 169
Cuts at: 2234 3018
Size: 784
Fragments arranged by size:
784 500 491 266 240 238 233 169
97
BmgBI CAC'GTC
Cuts at: 0 233 1623 2496 3018
Size: 233 1390 873 522
Fragments arranged by size:
1390 873 522 233
BmrI ACTGGGnnnn_n'
Cuts at: 0 232 948 3018
Size: 232 716 2070
Fragments arranged by size:
2070 716 232
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 884 2118 2238 3018
Size: 884 1234 120 780
Fragments arranged by size:
1234 884 780 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1120 1366 1374 1687 2090 2208 2323 3018
Size: 1120 246 8 313 403 118 115 695
Fragments arranged by size:
1120 695 403 313 246 118 115 8
Bpu10I CC'TnA_GC
Cuts at: 0 364 1450 2033 3018
Size: 364 1086 583 985
Fragments arranged by size:
1086 985 583 364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 672 3018
Size: 541 131 2346
Fragments arranged by size:
2346 541 131
BsaAI yAC'GTr
Cuts at: 0 387 792 1923 2478 3018
Size: 387 405 1131 555 540
Fragments arranged by size:
1131 555 540 405 387
BsaHI Gr'CG_yC
Cuts at: 0 168 1721 2462 2530 3018
Size: 168 1553 741 68 488
Fragments arranged by size:
1553 741 488 168 68
BsaWI w'CCGG_w
Cuts at: 0 674 1253 1439 3018
Size: 674 579 186 1579
Fragments arranged by size:
1579 674 579 186
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1339 1369 1930 1960 2638 2668 3018
Size: 1339 30 561 30 678 30 350
Fragments arranged by size:
1339 678 561 350 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 1231 1359 1377 1887 2115 2212 2329
Size: 88 1143 128 18 510 228 97 117
Cuts at: 2329 3018
Size: 689
Fragments arranged by size:
1143 689 510 228 128 117 97 88
18
BseYI C'CCAG_C
Cuts at: 0 660 1148 1324 1414 1875 2272 3018
Size: 660 488 176 90 461 397 746
Fragments arranged by size:
746 660 488 461 397 176 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 733 820 829 1377 1945 2365 2422
Size: 155 578 87 9 548 568 420 57
Cuts at: 2422 2490 2527 3016 3018
Size: 68 37 489 2
Fragments arranged by size:
578 568 548 489 420 155 87 68
57 37 9 2
BsiEI CG_ry'CG
Cuts at: 0 1440 3018
Size: 1440 1578
BsiHKAI G_wGCw'C
Cuts at: 0 1286 1565 1706 2489 2834 3018
Size: 1286 279 141 783 345 184
Fragments arranged by size:
1286 783 345 279 184 141
BsmI GAATG_Cn'
Cuts at: 0 1196 2085 3018
Size: 1196 889 933
Fragments arranged by size:
1196 933 889
BsmBI CGTCTCn'nnnn_
Cuts at: 0 159 2453 3018
Size: 159 2294 565
Fragments arranged by size:
2294 565 159
Bsp1286I G_dGCh'C
Cuts at: 0 238 340 729 962 1136 1228 1286 1325
Size: 238 102 389 233 174 92 58 39
Cuts at: 1325 1352 1369 1565 1706 2003 2065 2234 2264
Size: 27 17 196 141 297 62 169 30
Cuts at: 2264 2489 2834 3018
Size: 225 345 184
Fragments arranged by size:
389 345 297 238 233 225 196 184
174 169 141 102 92 62 58 39
30 27 17
BspEI T'CCGG_A
Cuts at: 0 674 1253 3018
Size: 674 579 1765
Fragments arranged by size:
1765 674 579
BspHI T'CATG_A
Cuts at: 0 893 2588 3018
Size: 893 1695 430
Fragments arranged by size:
1695 893 430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1085 1770 1803 2387 3018
Size: 1085 685 33 584 631
Fragments arranged by size:
1085 685 631 584 33
BsrBI CCG'CTC
Cuts at: 0 1004 1449 1863 3018
Size: 1004 445 414 1155
Fragments arranged by size:
1155 1004 445 414
BsrDI GCAATG_nn'
Cuts at: 0 119 3018
Size: 119 2899
BsrFI r'CCGG_y
Cuts at: 0 1439 1586 3018
Size: 1439 147 1432
Fragments arranged by size:
1439 1432 147
BssSI C'ACGA_G
Cuts at: 0 952 1279 1705 3018
Size: 952 327 426 1313
Fragments arranged by size:
1313 952 426 327
BstAPI GCAn_nnn'nTGC
Cuts at: 0 986 3018
Size: 986 2032
BstBI TT'CG_AA
Cuts at: 0 2192 3018
Size: 2192 826
BstEII G'GTnAC_C
Cuts at: 0 2541 3018
Size: 2541 477
BstYI r'GATC_y
Cuts at: 0 671 1709 2268 2666 2960 3018
Size: 671 1038 559 398 294 58
Fragments arranged by size:
1038 671 559 398 294 58
Bsu36I CC'TnA_GG
Cuts at: 0 303 371 1899 2288 3018
Size: 303 68 1528 389 730
Fragments arranged by size:
1528 730 389 303 68
BtgI C'CryG_G
Cuts at: 0 1205 1532 1604 2069 2090 2141 2627 3018
Size: 1205 327 72 465 21 51 486 391
Fragments arranged by size:
1205 486 465 391 327 72 51 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2832 3018
Size: 2832 186
BtsI GCAGTG_nn'
Cuts at: 0 143 198 478 984 1515 1796 3018
Size: 143 55 280 506 531 281 1222
Fragments arranged by size:
1222 531 506 281 280 143 55
DraIII CAC_nnn'GTG
Cuts at: 0 2994 3018
Size: 2994 24
DrdI GACnn_nn'nnGTC
Cuts at: 0 1548 3018
Size: 1548 1470
EaeI y'GGCC_r
Cuts at: 0 1269 1740 3018
Size: 1269 471 1278
Fragments arranged by size:
1278 1269 471
EarI CTCTTCn'nnn_
Cuts at: 0 524 1468 2189 2587 3018
Size: 524 944 721 398 431
Fragments arranged by size:
944 721 524 431 398
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 460 3018
Size: 460 2558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 948 1120 1366 1374 1687 2033 2090
Size: 892 56 172 246 8 313 346 57
Cuts at: 2090 2208 2323 2562 3018
Size: 118 115 239 456
Fragments arranged by size:
892 456 346 313 246 239 172 118
115 57 56 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1615 2170 2863 3018
Size: 1615 555 693 155
Fragments arranged by size:
1615 693 555 155
EcoO109I rG'GnC_Cy
Cuts at: 0 321 354 529 1598 2007 2047 2979 3018
Size: 321 33 175 1069 409 40 932 39
Fragments arranged by size:
1069 932 409 321 175 40 39 33
FspI TGC'GCA
Cuts at: 0 190 3018
Size: 190 2828
HaeII r_GCGC'y
Cuts at: 0 406 660 1558 1932 2533 3018
Size: 406 254 898 374 601 485
Fragments arranged by size:
898 601 485 406 374 254
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 552 584 1620 1652 2867 2899 3018
Size: 552 32 1036 32 1215 32 119
Fragments arranged by size:
1215 1036 552 119 32 32 32
KasI G'GCGC_C
Cuts at: 0 2529 3018
Size: 2529 489
KpnI G_GTAC'C
Cuts at: 0 1809 3018
Size: 1809 1209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 3018
Size: 683 2335
MscI TGG'CCA
Cuts at: 0 1742 3018
Size: 1742 1276
MslI CAynn'nnrTG
Cuts at: 0 220 1015 2068 2702 2948 3018
Size: 220 795 1053 634 246 70
Fragments arranged by size:
1053 795 634 246 220 70
MspA1I CmG'CkG
Cuts at: 0 162 178 347 609 1053 1140 1606 1857
Size: 162 16 169 262 444 87 466 251
Cuts at: 1857 2562 2573 3018
Size: 705 11 445
Fragments arranged by size:
705 466 445 444 262 251 169 162
87 16 11
NarI GG'CG_CC
Cuts at: 0 2530 3018
Size: 2530 488
NcoI C'CATG_G
Cuts at: 0 2627 3018
Size: 2627 391
NsiI A_TGCA'T
Cuts at: 0 2699 3018
Size: 2699 319
NspI r_CATG'y
Cuts at: 0 1408 2517 2652 3018
Size: 1408 1109 135 366
Fragments arranged by size:
1408 1109 366 135
PasI CC'CwG_GG
Cuts at: 0 666 3018
Size: 666 2352
PflMI CCAn_nnn'nTGG
Cuts at: 0 795 2670 3018
Size: 795 1875 348
Fragments arranged by size:
1875 795 348
PfoI T'CCnGG_A
Cuts at: 0 2669 3018
Size: 2669 349
PmlI CAC'GTG
Cuts at: 0 792 1923 3018
Size: 792 1131 1095
Fragments arranged by size:
1131 1095 792
PpuMI rG'GwC_Cy
Cuts at: 0 529 2007 2047 2979 3018
Size: 529 1478 40 932 39
Fragments arranged by size:
1478 932 529 40 39
PspOMI G'GGCC_C
Cuts at: 0 1321 2230 3018
Size: 1321 909 788
Fragments arranged by size:
1321 909 788
PstI C_TGCA'G
Cuts at: 0 278 457 858 2244 3018
Size: 278 179 401 1386 774
Fragments arranged by size:
1386 774 401 278 179
PvuII CAG'CTG
Cuts at: 0 162 2562 2573 3018
Size: 162 2400 11 445
Fragments arranged by size:
2400 445 162 11
SacII CC_GC'GG
Cuts at: 0 1607 3018
Size: 1607 1411
SanDI GG'GwC_CC
Cuts at: 0 529 3018
Size: 529 2489
ScaI AGT'ACT
Cuts at: 0 463 3018
Size: 463 2555
SfcI C'TryA_G
Cuts at: 0 274 453 854 1422 2240 2348 2568 3018
Size: 274 179 401 568 818 108 220 450
Fragments arranged by size:
818 568 450 401 274 220 179 108
SfoI GGC'GCC
Cuts at: 0 2531 3018
Size: 2531 487
SmaI CCC'GGG
Cuts at: 0 1492 1595 2052 2977 3018
Size: 1492 103 457 925 41
Fragments arranged by size:
1492 925 457 103 41
SmlI C'TyrA_G
Cuts at: 0 556 687 2446 3018
Size: 556 131 1759 572
Fragments arranged by size:
1759 572 556 131
StuI AGG'CCT
Cuts at: 0 376 1834 2869 3018
Size: 376 1458 1035 149
Fragments arranged by size:
1458 1035 376 149
StyI C'CwwG_G
Cuts at: 0 218 539 1616 2627 3018
Size: 218 321 1077 1011 391
Fragments arranged by size:
1077 1011 391 321 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1889 2650 2731 3018
Size: 1889 761 81 287
Fragments arranged by size:
1889 761 287 81
TatI w'GTAC_w
Cuts at: 0 461 3018
Size: 461 2557
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1066 1098 1339 1371 1930 1962 3018
Size: 1066 32 241 32 559 32 1056
Fragments arranged by size:
1066 1056 559 241 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 19 427 2983 3018
Size: 19 408 2556 35
Fragments arranged by size:
2556 408 35 19
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 895 3018
Size: 895 2123
XhoI C'TCGA_G
Cuts at: 0 2446 3018
Size: 2446 572
XmaI C'CCGG_G
Cuts at: 0 1490 1593 2050 2975 3018
Size: 1490 103 457 925 43
Fragments arranged by size:
1490 925 457 103 43
XmnI GAAnn'nnTTC
Cuts at: 0 1168 3018
Size: 1168 1850
ZraI GAC'GTC
Cuts at: 0 1722 2463 3018
Size: 1722 741 555
Fragments arranged by size:
1722 741 555
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I KasI KpnI MmeI MscI
MslI MspA1I NarI NcoI NsiI NspI PasI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII SacII SanDI
ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AhdI AloI ApoI AscI
AseI AsiSI AvrII BaeI BciVI BglII BmtI BsaI
BsaBI BsiWI BsrGI BssHII BstXI BstZ17I ClaI CspCI
DraI EagI EcoICRI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI PacI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SapI
SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI XbaI