Restriction map for
pF1KA0399
(Linear) (Six-base) MAPSORT of: KIAA0399.seq Check: 9464 from: 1 to: 8883
KIAA0399.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 936 6141 6557 8883
Size: 936 5205 416 2326
Fragments arranged by size:
5205 2326 936 416
AatII G_ACGT'C
Cuts at: 0 4679 8883
Size: 4679 4204
AccI GT'mk_AC
Cuts at: 0 6455 8883
Size: 6455 2428
Acc65I G'GTAC_C
Cuts at: 0 2102 8883
Size: 2102 6781
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 676 967 1579 1805 1867 2194 2956
Size: 427 249 291 612 226 62 327 762
Cuts at: 2956 3214 3744 4451 4559 4690 4795 6191 6730
Size: 258 530 707 108 131 105 1396 539
Cuts at: 6730 7111 7162 8256 8883
Size: 381 51 1094 627
Fragments arranged by size:
1396 1094 762 707 627 612 539 530
427 381 327 291 258 249 226 131
108 105 62 51
AfeI AGC'GCT
Cuts at: 0 128 2071 8883
Size: 128 1943 6812
Fragments arranged by size:
6812 1943 128
AflIII A'CryG_T
Cuts at: 0 404 629 4520 5300 8883
Size: 404 225 3891 780 3583
Fragments arranged by size:
3891 3583 780 404 225
AgeI A'CCGG_T
Cuts at: 0 5931 8883
Size: 5931 2952
AleI CACnn'nnGTG
Cuts at: 0 23 2502 2504 4715 8883
Size: 23 2479 2 2211 4168
Fragments arranged by size:
4168 2479 2211 23 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1529 4272 6257 7751 8834 8883
Size: 1529 2743 1985 1494 1083 49
Fragments arranged by size:
2743 1985 1529 1494 1083 49
AlwNI CAG_nnn'CTG
Cuts at: 0 848 916 958 1557 2676 3003 3895 6169
Size: 848 68 42 599 1119 327 892 2274
Cuts at: 6169 7698 8808 8883
Size: 1529 1110 75
Fragments arranged by size:
2274 1529 1119 1110 892 848 599 327
75 68 42
ApaI G_GGCC'C
Cuts at: 0 67 2856 6310 6951 8883
Size: 67 2789 3454 641 1932
Fragments arranged by size:
3454 2789 1932 641 67
ApaLI G'TGCA_C
Cuts at: 0 633 1000 1479 2147 2700 8883
Size: 633 367 479 668 553 6183
Fragments arranged by size:
6183 668 633 553 479 367
ApoI r'AATT_y
Cuts at: 0 423 1115 1828 2316 3370 3415 3559 4285
Size: 423 692 713 488 1054 45 144 726
Cuts at: 4285 4928 5205 6119 7211 8185 8477 8883
Size: 643 277 914 1092 974 292 406
Fragments arranged by size:
1092 1054 974 914 726 713 692 643
488 423 406 292 277 144 45
AvaI C'yCGr_G
Cuts at: 0 90 108 2343 2822 4586 5907 6944 7027
Size: 90 18 2235 479 1764 1321 1037 83
Cuts at: 7027 8883
Size: 1856
Fragments arranged by size:
2235 1856 1764 1321 1037 479 90 83
18
AvrII C'CTAG_G
Cuts at: 0 5430 8883
Size: 5430 3453
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 5373 5406 8883
Size: 5373 33 3477
Fragments arranged by size:
5373 3477 33
BamHI G'GATC_C
Cuts at: 0 2721 6099 8883
Size: 2721 3378 2784
Fragments arranged by size:
3378 2784 2721
BanI G'GyrC_C
Cuts at: 0 322 896 1783 2102 4474 4638 8164 8883
Size: 322 574 887 319 2372 164 3526 719
Fragments arranged by size:
3526 2372 887 719 574 322 319 164
BanII G_rGCy'C
Cuts at: 0 67 1474 1892 2856 2872 4547 5914 6310
Size: 67 1407 418 964 16 1675 1367 396
Cuts at: 6310 6951 7385 7523 7928 8705 8883
Size: 641 434 138 405 777 178
Fragments arranged by size:
1675 1407 1367 964 777 641 434 418
405 396 178 138 67 16
BbeI G_GCGC'C
Cuts at: 0 326 8883
Size: 326 8557
BbsI GAAGACnn'nnnn_
Cuts at: 0 35 638 3750 4264 6406 8883
Size: 35 603 3112 514 2142 2477
Fragments arranged by size:
3112 2477 2142 603 514 35
BbvCI CC'TCA_GC
Cuts at: 0 374 8883
Size: 374 8509
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 559 593 5805 5839 8707 8741 8883
Size: 559 34 5212 34 2868 34 142
Fragments arranged by size:
5212 2868 559 142 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3339 7532 7605 8883
Size: 3339 4193 73 1278
Fragments arranged by size:
4193 3339 1278 73
BclI T'GATC_A
Cuts at: 0 3999 5159 5513 6467 6818 8883
Size: 3999 1160 354 954 351 2065
Fragments arranged by size:
3999 2065 1160 954 354 351
BglI GCCn_nnn'nGGC
Cuts at: 0 162 5737 6590 8516 8613 8883
Size: 162 5575 853 1926 97 270
Fragments arranged by size:
5575 1926 853 270 162 97
BglII A'GATC_T
Cuts at: 0 705 2306 2961 5109 8883
Size: 705 1601 655 2148 3774
Fragments arranged by size:
3774 2148 1601 705 655
BlpI GC'TnA_GC
Cuts at: 0 454 5993 6955 7262 7553 8883
Size: 454 5539 962 307 291 1330
Fragments arranged by size:
5539 1330 962 454 307 291
Bme1580I G_kGCm'C
Cuts at: 0 67 97 637 1004 1483 1788 2151 2543
Size: 67 30 540 367 479 305 363 392
Cuts at: 2543 2704 2856 4477 4643 5945 6310 6645 6951
Size: 161 152 1621 166 1302 365 335 306
Cuts at: 6951 8694 8730 8883
Size: 1743 36 153
Fragments arranged by size:
1743 1621 1302 540 479 392 367 365
363 335 306 305 166 161 153 152
67 36 30
BmgBI CAC'GTC
Cuts at: 0 1771 4521 6339 8655 8883
Size: 1771 2750 1818 2316 228
Fragments arranged by size:
2750 2316 1818 1771 228
BmrI ACTGGGnnnn_n'
Cuts at: 0 87 357 1782 3651 3879 6609 7562 8883
Size: 87 270 1425 1869 228 2730 953 1321
Fragments arranged by size:
2730 1869 1425 1321 953 270 228 87
BmtI G_CTAG'C
Cuts at: 0 6146 8883
Size: 6146 2737
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2886 4612 8883
Size: 2886 1726 4271
Fragments arranged by size:
4271 2886 1726
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 172 310 331 570 634 722 1567
Size: 107 65 138 21 239 64 88 845
Cuts at: 1567 2374 2972 4028 4342 6352 7510 7689 7942
Size: 807 598 1056 314 2010 1158 179 253
Cuts at: 7942 8002 8883
Size: 60 881
Fragments arranged by size:
2010 1158 1056 881 845 807 598 314
253 239 179 138 107 88 65 64
60 21
Bpu10I CC'TnA_GC
Cuts at: 0 374 892 1891 3733 6230 8883
Size: 374 518 999 1842 2497 2653
Fragments arranged by size:
2653 2497 1842 999 518 374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 721 3183 3655 7387 8883
Size: 403 318 2462 472 3732 1496
Fragments arranged by size:
3732 2462 1496 472 403 318
BsaI GGTCTCn'nnnn_
Cuts at: 0 93 1129 1170 1205 3238 4448 7265 8285
Size: 93 1036 41 35 2033 1210 2817 1020
Cuts at: 8285 8883
Size: 598
Fragments arranged by size:
2817 2033 1210 1036 1020 598 93 41
35
BsaAI yAC'GTr
Cuts at: 0 2705 3281 7863 7899 8883
Size: 2705 576 4582 36 984
Fragments arranged by size:
4582 2705 984 576 36
BsaBI GATnn'nnATC
Cuts at: 0 2773 8883
Size: 2773 6110
BsaHI Gr'CG_yC
Cuts at: 0 113 323 1540 3645 4676 6458 8883
Size: 113 210 1217 2105 1031 1782 2425
Fragments arranged by size:
2425 2105 1782 1217 1031 210 113
BsaWI w'CCGG_w
Cuts at: 0 1720 3818 5931 5947 8883
Size: 1720 2098 2113 16 2936
Fragments arranged by size:
2936 2113 2098 1720 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2522 2552 5096 5126 8883
Size: 2522 30 2544 30 3757
Fragments arranged by size:
3757 2544 2522 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 364 1552 2185 2188 2452 3613 4639 5104
Size: 364 1188 633 3 264 1161 1026 465
Cuts at: 5104 5974 6935 7186 7224 8092 8224 8883
Size: 870 961 251 38 868 132 659
Fragments arranged by size:
1188 1161 1026 961 870 868 659 633
465 364 264 251 132 38 3
BseYI C'CCAG_C
Cuts at: 0 59 261 1009 4478 5063 5883 6950 6968
Size: 59 202 748 3469 585 820 1067 18
Cuts at: 6968 8040 8883
Size: 1072 843
Fragments arranged by size:
3469 1072 1067 843 820 748 585 202
59 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 1249 2031 2263 3117 4814 5418 5765
Size: 553 696 782 232 854 1697 604 347
Cuts at: 5765 7182 7417 7634 7756 8883
Size: 1417 235 217 122 1127
Fragments arranged by size:
1697 1417 1127 854 782 696 604 553
347 235 232 217 122
BsiEI CG_ry'CG
Cuts at: 0 8387 8883
Size: 8387 496
BsiHKAI G_wGCw'C
Cuts at: 0 620 637 1004 1474 1483 1892 2151 2666
Size: 620 17 367 470 9 409 259 515
Cuts at: 2666 2704 2944 3161 6338 6610 7268 7523 7928
Size: 38 240 217 3177 272 658 255 405
Cuts at: 7928 8018 8705 8883
Size: 90 687 178
Fragments arranged by size:
3177 687 658 620 515 470 409 405
367 272 259 255 240 217 178 90
38 17 9
BsmI GAATG_Cn'
Cuts at: 0 975 2359 8883
Size: 975 1384 6524
Fragments arranged by size:
6524 1384 975
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2364 2814 3633 5453 8883
Size: 2364 450 819 1820 3430
Fragments arranged by size:
3430 2364 1820 819 450
Bsp1286I G_dGCh'C
Cuts at: 0 67 97 620 637 1004 1474 1483 1788
Size: 67 30 523 17 367 470 9 305
Cuts at: 1788 1892 2151 2543 2666 2704 2856 2872 2944
Size: 104 259 392 123 38 152 16 72
Cuts at: 2944 3161 4477 4547 4643 5914 5945 6310 6338
Size: 217 1316 70 96 1271 31 365 28
Cuts at: 6338 6610 6645 6951 7268 7385 7523 7928 8018
Size: 272 35 306 317 117 138 405 90
Cuts at: 8018 8694 8705 8730 8883
Size: 676 11 25 153
Fragments arranged by size:
1316 1271 676 523 470 405 392 367
365 317 306 305 272 259 217 153
152 138 123 117 104 96 90 72
70 67 38 35 31 30 28 25
17 16 11 9
BspHI T'CATG_A
Cuts at: 0 1466 5117 6030 7410 8883
Size: 1466 3651 913 1380 1473
Fragments arranged by size:
3651 1473 1466 1380 913
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 936 1565 2936 4870 6141 6557 8765 8883
Size: 936 629 1371 1934 1271 416 2208 118
Fragments arranged by size:
2208 1934 1371 1271 936 629 416 118
BsrBI CCG'CTC
Cuts at: 0 258 8883
Size: 258 8625
BsrDI GCAATG_nn'
Cuts at: 0 905 1020 5306 5544 6018 6662 8816 8883
Size: 905 115 4286 238 474 644 2154 67
Fragments arranged by size:
4286 2154 905 644 474 238 115 67
BsrFI r'CCGG_y
Cuts at: 0 46 156 325 3499 4571 5657 5931 8883
Size: 46 110 169 3174 1072 1086 274 2952
Fragments arranged by size:
3174 2952 1086 1072 274 169 110 46
BsrGI T'GTAC_A
Cuts at: 0 2492 8883
Size: 2492 6391
BssSI C'ACGA_G
Cuts at: 0 6736 8883
Size: 6736 2147
BstAPI GCAn_nnn'nTGC
Cuts at: 0 916 2676 4444 5423 5543 8013 8883
Size: 916 1760 1768 979 120 2470 870
Fragments arranged by size:
2470 1768 1760 979 916 870 120
BstBI TT'CG_AA
Cuts at: 0 4143 8883
Size: 4143 4740
BstEII G'GTnAC_C
Cuts at: 0 5637 8883
Size: 5637 3246
BstXI CCAn_nnnn'nTGG
Cuts at: 0 912 4465 6533 7608 8055 8737 8832 8883
Size: 912 3553 2068 1075 447 682 95 51
Fragments arranged by size:
3553 2068 1075 912 682 447 95 51
BstYI r'GATC_y
Cuts at: 0 705 1318 2306 2721 2961 5109 5274 5409
Size: 705 613 988 415 240 2148 165 135
Cuts at: 5409 6099 6620 7109 8000 8883
Size: 690 521 489 891 883
Fragments arranged by size:
2148 988 891 883 705 690 613 521
489 415 240 165 135
Bsu36I CC'TnA_GG
Cuts at: 0 2200 3710 4738 5447 8883
Size: 2200 1510 1028 709 3436
Fragments arranged by size:
3436 2200 1510 1028 709
BtgI C'CryG_G
Cuts at: 0 83 140 267 1535 1657 2543 2969 4295
Size: 83 57 127 1268 122 886 426 1326
Cuts at: 4295 4619 5454 6311 6611 8048 8771 8883
Size: 324 835 857 300 1437 723 112
Fragments arranged by size:
1437 1326 1268 886 857 835 723 426
324 300 127 122 112 83 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 211 1112 5561 8577 8883
Size: 211 901 4449 3016 306
Fragments arranged by size:
4449 3016 901 306 211
BtsI GCAGTG_nn'
Cuts at: 0 30 618 849 917 2151 3150 3569 4506
Size: 30 588 231 68 1234 999 419 937
Cuts at: 4506 4815 5401 5499 5796 6470 6681 7667 8250
Size: 309 586 98 297 674 211 986 583
Cuts at: 8250 8883
Size: 633
Fragments arranged by size:
1234 999 986 937 674 633 588 586
583 419 309 297 231 211 98 68
30
ClaI AT'CG_AT
Cuts at: 0 5379 8883
Size: 5379 3504
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4373 4408 8883
Size: 4373 35 4475
Fragments arranged by size:
4475 4373 35
DraI TTT'AAA
Cuts at: 0 869 8883
Size: 869 8014
DraIII CAC_nnn'GTG
Cuts at: 0 3156 7657 8360 8883
Size: 3156 4501 703 523
Fragments arranged by size:
4501 3156 703 523
DrdI GACnn_nn'nnGTC
Cuts at: 0 2806 8883
Size: 2806 6077
EaeI y'GGCC_r
Cuts at: 0 158 2762 5598 5738 6420 7318 8384 8517
Size: 158 2604 2836 140 682 898 1066 133
Cuts at: 8517 8571 8834 8883
Size: 54 263 49
Fragments arranged by size:
2836 2604 1066 898 682 263 158 140
133 54 49
EagI C'GGCC_G
Cuts at: 0 8384 8883
Size: 8384 499
EarI CTCTTCn'nnn_
Cuts at: 0 248 269 1430 1598 2638 2884 2932 4100
Size: 248 21 1161 168 1040 246 48 1168
Cuts at: 4100 4261 4394 4535 6253 6874 7301 7495 7825
Size: 161 133 141 1718 621 427 194 330
Cuts at: 7825 8710 8883
Size: 885 173
Fragments arranged by size:
1718 1168 1161 1040 885 621 427 330
248 246 194 173 168 161 141 133
48 21
EcoICRI GAG'CTC
Cuts at: 0 1472 1890 7521 7926 8703 8883
Size: 1472 418 5631 405 777 180
Fragments arranged by size:
5631 1472 777 418 405 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 172 310 331 427 570 634 676
Size: 107 65 138 21 96 143 64 42
Cuts at: 676 722 967 1567 1579 1805 1867 2194 2374
Size: 46 245 600 12 226 62 327 180
Cuts at: 2374 2956 2972 3214 3744 4028 4342 4451 4559
Size: 582 16 242 530 284 314 109 108
Cuts at: 4559 4690 4795 6191 6352 6730 7111 7162 7510
Size: 131 105 1396 161 378 381 51 348
Cuts at: 7510 7689 7942 8002 8256 8883
Size: 179 253 60 254 627
Fragments arranged by size:
1396 627 600 582 530 381 378 348
327 314 284 254 253 245 242 226
180 179 161 143 138 131 109 108
107 105 96 65 64 62 60 51
46 42 21 16 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1939 4448 7774 8647 8883
Size: 1939 2509 3326 873 236
Fragments arranged by size:
3326 2509 1939 873 236
EcoO109I rG'GnC_Cy
Cuts at: 0 63 414 531 2204 2852 2853 3963 5003
Size: 63 351 117 1673 648 1 1110 1040
Cuts at: 5003 5925 6307 6759 7050 7203 7371 8883
Size: 922 382 452 291 153 168 1512
Fragments arranged by size:
1673 1512 1110 1040 922 648 452 382
351 291 168 153 117 63 1
EcoRI G'AATT_C
Cuts at: 0 423 1115 3370 5205 8883
Size: 423 692 2255 1835 3678
Fragments arranged by size:
3678 2255 1835 692 423
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 5203 8883
Size: 5203 3680
HaeII r_GCGC'y
Cuts at: 0 130 148 326 1285 2073 3670 5732 7601
Size: 130 18 178 959 788 1597 2062 1869
Cuts at: 7601 7749 7763 8883
Size: 148 14 1120
Fragments arranged by size:
2062 1869 1597 1120 959 788 178 148
130 18 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 529 561 1504 1536 2518 2550 4534 4566
Size: 529 32 943 32 982 32 1984 32
Cuts at: 4566 5832 5864 5890 5922 8505 8537 8883
Size: 1266 32 26 32 2583 32 346
Fragments arranged by size:
2583 1984 1266 982 943 529 346 32
32 32 32 32 32 32 26
HincII GTy'rAC
Cuts at: 0 1589 3106 4077 5478 8883
Size: 1589 1517 971 1401 3405
Fragments arranged by size:
3405 1589 1517 1401 971
HindIII A'AGCT_T
Cuts at: 0 1879 8883
Size: 1879 7004
KasI G'GCGC_C
Cuts at: 0 322 8883
Size: 322 8561
KpnI G_GTAC'C
Cuts at: 0 2106 8883
Size: 2106 6777
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 803 3415 3764 4310 4829 8366 8883
Size: 389 414 2612 349 546 519 3537 517
Fragments arranged by size:
3537 2612 546 519 517 414 389 349
MscI TGG'CCA
Cuts at: 0 2764 5740 8519 8836 8883
Size: 2764 2976 2779 317 47
Fragments arranged by size:
2976 2779 2764 317 47
MslI CAynn'nnrTG
Cuts at: 0 23 910 2502 2504 2641 3305 4715 5122
Size: 23 887 1592 2 137 664 1410 407
Cuts at: 5122 5153 6386 8883
Size: 31 1233 2497
Fragments arranged by size:
2497 1592 1410 1233 887 664 407 137
31 23 2
MspA1I CmG'CkG
Cuts at: 0 43 85 142 269 675 2934 4537 5387
Size: 43 42 57 127 406 2259 1603 850
Cuts at: 5387 6696 6708 6779 6906 6954 7557 7843 7974
Size: 1309 12 71 127 48 603 286 131
Cuts at: 7974 8276 8792 8883
Size: 302 516 91
Fragments arranged by size:
2259 1603 1309 850 603 516 406 302
286 131 127 127 91 71 57 48
43 42 12
NaeI GCC'GGC
Cuts at: 0 158 327 4573 8883
Size: 158 169 4246 4310
Fragments arranged by size:
4310 4246 169 158
NarI GG'CG_CC
Cuts at: 0 323 8883
Size: 323 8560
NcoI C'CATG_G
Cuts at: 0 1535 2543 4619 6611 8048 8883
Size: 1535 1008 2076 1992 1437 835
Fragments arranged by size:
2076 1992 1535 1437 1008 835
NdeI CA'TA_TG
Cuts at: 0 7943 8883
Size: 7943 940
NgoMIV G'CCGG_C
Cuts at: 0 156 325 4571 8883
Size: 156 169 4246 4312
Fragments arranged by size:
4312 4246 169 156
NheI G'CTAG_C
Cuts at: 0 6142 8883
Size: 6142 2741
NspI r_CATG'y
Cuts at: 0 408 633 939 1296 1604 3953 5241 5304
Size: 408 225 306 357 308 2349 1288 63
Cuts at: 5304 5820 8040 8066 8883
Size: 516 2220 26 817
Fragments arranged by size:
2349 2220 1288 817 516 408 357 308
306 225 63 26
PasI CC'CwG_GG
Cuts at: 0 2857 3346 6408 8883
Size: 2857 489 3062 2475
Fragments arranged by size:
3062 2857 2475 489
PciI A'CATG_T
Cuts at: 0 404 629 5300 8883
Size: 404 225 4671 3583
Fragments arranged by size:
4671 3583 404 225
PflMI CCAn_nnn'nTGG
Cuts at: 0 2238 3003 3663 5472 5634 8073 8883
Size: 2238 765 660 1809 162 2439 810
Fragments arranged by size:
2439 2238 1809 810 765 660 162
PfoI T'CCnGG_A
Cuts at: 0 299 4763 7199 7487 7808 7919 8310 8883
Size: 299 4464 2436 288 321 111 391 573
Fragments arranged by size:
4464 2436 573 391 321 299 288 111
PmlI CAC'GTG
Cuts at: 0 7863 7899 8883
Size: 7863 36 984
Fragments arranged by size:
7863 984 36
PpuMI rG'GwC_Cy
Cuts at: 0 2204 5003 6759 7050 7203 8883
Size: 2204 2799 1756 291 153 1680
Fragments arranged by size:
2799 2204 1756 1680 291 153
PshAI GACnn'nnGTC
Cuts at: 0 3536 4460 8883
Size: 3536 924 4423
Fragments arranged by size:
4423 3536 924
PspOMI G'GGCC_C
Cuts at: 0 63 2852 6306 6947 8883
Size: 63 2789 3454 641 1936
Fragments arranged by size:
3454 2789 1936 641 63
PspXI vC'TCGA_Gb
Cuts at: 0 5907 8883
Size: 5907 2976
PstI C_TGCA'G
Cuts at: 0 183 2158 2384 2414 2681 2933 4518 5396
Size: 183 1975 226 30 267 252 1585 878
Cuts at: 5396 6138 6507 7463 7727 8009 8750 8813 8883
Size: 742 369 956 264 282 741 63 70
Fragments arranged by size:
1975 1585 956 878 742 741 369 282
267 264 252 226 183 70 63 30
PvuII CAG'CTG
Cuts at: 0 43 675 2934 4537 6696 6708 6906 6954
Size: 43 632 2259 1603 2159 12 198 48
Cuts at: 6954 7843 7974 8883
Size: 889 131 909
Fragments arranged by size:
2259 2159 1603 909 889 632 198 131
48 43 12
SacI G_AGCT'C
Cuts at: 0 1474 1892 7523 7928 8705 8883
Size: 1474 418 5631 405 777 178
Fragments arranged by size:
5631 1474 777 418 405 178
SacII CC_GC'GG
Cuts at: 0 86 143 270 8883
Size: 86 57 127 8613
Fragments arranged by size:
8613 127 86 57
SapI GCTCTTCn'nnn_
Cuts at: 0 248 2884 2932 4394 4535 8710 8883
Size: 248 2636 48 1462 141 4175 173
Fragments arranged by size:
4175 2636 1462 248 173 141 48
ScaI AGT'ACT
Cuts at: 0 6964 8883
Size: 6964 1919
SexAI A'CCwGG_T
Cuts at: 0 2237 8883
Size: 2237 6646
SfcI C'TryA_G
Cuts at: 0 179 475 959 1322 2154 2380 2410 2677
Size: 179 296 484 363 832 226 30 267
Cuts at: 2677 2929 3204 4514 5392 5724 6047 6134 6152
Size: 252 275 1310 878 332 323 87 18
Cuts at: 6152 6503 7459 7723 8005 8367 8746 8809 8883
Size: 351 956 264 282 362 379 63 74
Fragments arranged by size:
1310 956 878 832 484 379 363 362
351 332 323 296 282 275 267 264
252 226 179 87 74 63 30 18
SfoI GGC'GCC
Cuts at: 0 324 8883
Size: 324 8559
SmaI CCC'GGG
Cuts at: 0 110 8883
Size: 110 8773
SmlI C'TyrA_G
Cuts at: 0 418 736 2822 3162 3670 5907 7366 8883
Size: 418 318 2086 340 508 2237 1459 1517
Fragments arranged by size:
2237 2086 1517 1459 508 418 340 318
SphI G_CATG'C
Cuts at: 0 5820 8066 8883
Size: 5820 2246 817
Fragments arranged by size:
5820 2246 817
SrfI GCCC'GGGC
Cuts at: 0 110 8883
Size: 110 8773
SspI AAT'ATT
Cuts at: 0 2026 8883
Size: 2026 6857
StuI AGG'CCT
Cuts at: 0 355 481 5248 5864 8883
Size: 355 126 4767 616 3019
Fragments arranged by size:
4767 3019 616 355 126
StyI C'CwwG_G
Cuts at: 0 1535 1935 2483 2543 2901 4127 4619 4803
Size: 1535 400 548 60 358 1226 492 184
Cuts at: 4803 5430 6611 8048 8883
Size: 627 1181 1437 835
Fragments arranged by size:
1535 1437 1226 1181 835 627 548 492
400 358 184 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3827 4492 4578 5342 5645 5875 6559 7147
Size: 3827 665 86 764 303 230 684 588
Cuts at: 7147 7313 7489 8276 8485 8883
Size: 166 176 787 209 398
Fragments arranged by size:
3827 787 764 684 665 588 398 303
230 209 176 166 86
TatI w'GTAC_w
Cuts at: 0 2421 2492 3044 6962 7229 8883
Size: 2421 71 552 3918 267 1654
Fragments arranged by size:
3918 2421 1654 552 267 71
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2522 2554 2656 2688 6589 6621 8078 8110
Size: 2522 32 102 32 3901 32 1457 32
Cuts at: 8110 8883
Size: 773
Fragments arranged by size:
3901 2522 1457 773 102 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 74 2028 8883
Size: 74 1954 6855
Fragments arranged by size:
6855 1954 74
XhoI C'TCGA_G
Cuts at: 0 2822 5907 8883
Size: 2822 3085 2976
Fragments arranged by size:
3085 2976 2822
XmaI C'CCGG_G
Cuts at: 0 108 8883
Size: 108 8775
ZraI GAC'GTC
Cuts at: 0 4677 8883
Size: 4677 4206
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PstI PvuII SacI SacII SapI
ScaI SexAI SfcI SfoI SmaI SmlI SphI SrfI
SspI StuI StyI TaqII TatI TstI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AclI AflII AhdI AloI AscI AseI AsiSI BfrBI
BsiWI BspEI BssHII BstZ17I EciI EcoRV FseI FspI
FspAI HpaI MfeI MluI NotI NruI NsiI PacI
PmeI PpiI PsiI PsrI PvuI RsrII SalI SanDI
SbfI SfiI SgfI SgrAI SnaBI SpeI SwaI Tth111I
XbaI XmnI