Restriction map for
pF1KA0404
(Linear) (Six-base) MAPSORT of: KIAA0404.seq Check: 3027 from: 1 to: 5814
KIAA0404.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 866 2098 4233 5814
Size: 866 1232 2135 1581
Fragments arranged by size:
2135 1581 1232 866
AatII G_ACGT'C
Cuts at: 0 1324 5814
Size: 1324 4490
AccI GT'mk_AC
Cuts at: 0 1160 2486 3938 5814
Size: 1160 1326 1452 1876
Fragments arranged by size:
1876 1452 1326 1160
Acc65I G'GTAC_C
Cuts at: 0 2893 5814
Size: 2893 2921
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1300 2818 2886 3884 4974 5140 5814
Size: 874 426 1518 68 998 1090 166 674
Fragments arranged by size:
1518 1090 998 874 674 426 166 68
AfeI AGC'GCT
Cuts at: 0 4685 5497 5814
Size: 4685 812 317
Fragments arranged by size:
4685 812 317
AflIII A'CryG_T
Cuts at: 0 3131 3654 5814
Size: 3131 523 2160
Fragments arranged by size:
3131 2160 523
AgeI A'CCGG_T
Cuts at: 0 5405 5814
Size: 5405 409
AhdI GACnn_n'nnGTC
Cuts at: 0 1511 5454 5814
Size: 1511 3943 360
Fragments arranged by size:
3943 1511 360
AleI CACnn'nnGTG
Cuts at: 0 523 1480 3145 3665 4252 4634 5814
Size: 523 957 1665 520 587 382 1180
Fragments arranged by size:
1665 1180 957 587 523 520 382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1498 1558 3683 4228 5814
Size: 1498 60 2125 545 1586
Fragments arranged by size:
2125 1586 1498 545 60
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2212 2244 5814
Size: 2212 32 3570
Fragments arranged by size:
3570 2212 32
AlwNI CAG_nnn'CTG
Cuts at: 0 343 447 591 897 999 1011 1030 1689
Size: 343 104 144 306 102 12 19 659
Cuts at: 1689 4510 4530 4575 5383 5814
Size: 2821 20 45 808 431
Fragments arranged by size:
2821 808 659 431 343 306 144 104
102 45 20 19 12
ApaI G_GGCC'C
Cuts at: 0 1397 1797 3969 3997 5248 5814
Size: 1397 400 2172 28 1251 566
Fragments arranged by size:
2172 1397 1251 566 400 28
ApaLI G'TGCA_C
Cuts at: 0 1543 2860 3658 5814
Size: 1543 1317 798 2156
Fragments arranged by size:
2156 1543 1317 798
AvaI C'yCGr_G
Cuts at: 0 870 1406 1583 1916 2577 3099 3241 4121
Size: 870 536 177 333 661 522 142 880
Cuts at: 4121 4250 4864 4871 5734 5814
Size: 129 614 7 863 80
Fragments arranged by size:
880 870 863 661 614 536 522 333
177 142 129 80 7
BanI G'GyrC_C
Cuts at: 0 630 967 1588 2893 4221 4283 5650 5814
Size: 630 337 621 1305 1328 62 1367 164
Fragments arranged by size:
1367 1328 1305 630 621 337 164 62
BanII G_rGCy'C
Cuts at: 0 235 428 716 743 850 1037 1145 1397
Size: 235 193 288 27 107 187 108 252
Cuts at: 1397 1642 1797 1871 2027 2321 3969 3997 4360
Size: 245 155 74 156 294 1648 28 363
Cuts at: 4360 4878 5153 5248 5814
Size: 518 275 95 566
Fragments arranged by size:
1648 566 518 363 294 288 275 252
245 235 193 187 156 155 108 107
95 74 28 27
BbeI G_GCGC'C
Cuts at: 0 634 5654 5814
Size: 634 5020 160
Fragments arranged by size:
5020 634 160
BbsI GAAGACnn'nnnn_
Cuts at: 0 980 2749 5814
Size: 980 1769 3065
Fragments arranged by size:
3065 1769 980
BbvCI CC'TCA_GC
Cuts at: 0 97 1039 1128 1650 4853 5814
Size: 97 942 89 522 3203 961
Fragments arranged by size:
3203 961 942 522 97 89
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1360 1394 3661 3695 5814
Size: 1360 34 2267 34 2119
Fragments arranged by size:
2267 2119 1360 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 5583 5814
Size: 5583 231
BfrBI ATG'CAT
Cuts at: 0 371 5814
Size: 371 5443
BglI GCCn_nnn'nGGC
Cuts at: 0 1993 2597 2746 3110 4001 4755 5716 5814
Size: 1993 604 149 364 891 754 961 98
Fragments arranged by size:
1993 961 891 754 604 364 149 98
BglII A'GATC_T
Cuts at: 0 2541 5814
Size: 2541 3273
BlpI GC'TnA_GC
Cuts at: 0 112 901 1031 1841 1997 2044 3044 4456
Size: 112 789 130 810 156 47 1000 1412
Cuts at: 4456 5814
Size: 1358
Fragments arranged by size:
1412 1358 1000 810 789 156 130 112
47
Bme1580I G_kGCm'C
Cuts at: 0 254 1397 1477 1547 1591 1797 2864 3662
Size: 254 1143 80 70 44 206 1067 798
Cuts at: 3662 3684 3811 3969 3997 4224 4244 4659 5071
Size: 22 127 158 28 227 20 415 412
Cuts at: 5071 5248 5808 5814
Size: 177 560 6
Fragments arranged by size:
1143 1067 798 560 415 412 254 227
206 177 158 127 80 70 44 28
22 20 6
BmgBI CAC'GTC
Cuts at: 0 1782 3282 4654 5009 5049 5613 5723 5814
Size: 1782 1500 1372 355 40 564 110 91
Fragments arranged by size:
1782 1500 1372 564 355 110 91 40
BmrI ACTGGGnnnn_n'
Cuts at: 0 2022 2059 2189 2220 2447 2907 3002 3020
Size: 2022 37 130 31 227 460 95 18
Cuts at: 3020 3255 3379 3808 4231 5814
Size: 235 124 429 423 1583
Fragments arranged by size:
2022 1583 460 429 423 235 227 130
124 95 37 31 18
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4609 5814
Size: 4609 1205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 229 286 469 478 613 719 865
Size: 208 21 57 183 9 135 106 146
Cuts at: 865 1579 1588 1832 2032 2296 2396 2872 3408
Size: 714 9 244 200 264 100 476 536
Cuts at: 3408 3685 4552 4597 5814
Size: 277 867 45 1217
Fragments arranged by size:
1217 867 714 536 476 277 264 244
208 200 183 146 135 106 100 57
45 21 9 9
Bpu10I CC'TnA_GC
Cuts at: 0 97 765 1039 1128 1650 1961 2315 3460
Size: 97 668 274 89 522 311 354 1145
Cuts at: 3460 4008 4853 5814
Size: 548 845 961
Fragments arranged by size:
1145 961 845 668 548 522 354 311
274 97 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4774 5770 5814
Size: 4774 996 44
Fragments arranged by size:
4774 996 44
BsaI GGTCTCn'nnnn_
Cuts at: 0 2372 2818 3403 3508 4275 4580 4837 4912
Size: 2372 446 585 105 767 305 257 75
Cuts at: 4912 5431 5814
Size: 519 383
Fragments arranged by size:
2372 767 585 519 446 383 305 257
105 75
BsaAI yAC'GTr
Cuts at: 0 3657 3708 4264 5814
Size: 3657 51 556 1550
Fragments arranged by size:
3657 1550 556 51
BsaBI GATnn'nnATC
Cuts at: 0 647 2368 5814
Size: 647 1721 3446
Fragments arranged by size:
3446 1721 647
BsaHI Gr'CG_yC
Cuts at: 0 631 1321 3854 3922 5651 5765 5814
Size: 631 690 2533 68 1729 114 49
Fragments arranged by size:
2533 1729 690 631 114 68 49
BsaWI w'CCGG_w
Cuts at: 0 420 1336 2033 5405 5814
Size: 420 916 697 3372 409
Fragments arranged by size:
3372 916 697 420 409
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1153 1177 1183 1207 1561 1591 3334 3364
Size: 1153 24 6 24 354 30 1743 30
Cuts at: 3364 3365 3395 4945 4975 5707 5737 5814
Size: 1 30 1550 30 732 30 77
Fragments arranged by size:
1743 1550 1153 732 354 77 30 30
30 30 30 24 24 6 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 724 1161 2322 2374 2401 4039 4063 4549
Size: 724 437 1161 52 27 1638 24 486
Cuts at: 4549 4590 5074 5814
Size: 41 484 740
Fragments arranged by size:
1638 1161 740 724 486 484 437 52
41 27 24
BseYI C'CCAG_C
Cuts at: 0 359 843 1221 1635 1788 2473 2570 3300
Size: 359 484 378 414 153 685 97 730
Cuts at: 3300 3748 3893 4243 4488 4519 4877 4882 5172
Size: 448 145 350 245 31 358 5 290
Cuts at: 5172 5232 5814
Size: 60 582
Fragments arranged by size:
730 685 582 484 448 414 378 359
358 350 290 245 153 145 97 60
31 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 264 604 652 685 838 1504 1895
Size: 46 218 340 48 33 153 666 391
Cuts at: 1895 3319 3712 4588 5814
Size: 1424 393 876 1226
Fragments arranged by size:
1424 1226 876 666 393 391 340 218
153 48 46 33
BsiEI CG_ry'CG
Cuts at: 0 2987 4195 4199 4543 5814
Size: 2987 1208 4 344 1271
Fragments arranged by size:
2987 1271 1208 344 4
BsiHKAI G_wGCw'C
Cuts at: 0 95 539 716 1145 1547 2179 2773 2864
Size: 95 444 177 429 402 632 594 91
Cuts at: 2864 2891 3598 3662 3718 4360 5814
Size: 27 707 64 56 642 1454
Fragments arranged by size:
1454 707 642 632 594 444 429 402
177 95 91 64 56 27
BsmI GAATG_Cn'
Cuts at: 0 3255 5814
Size: 3255 2559
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2913 3814 4743 5478 5814
Size: 2913 901 929 735 336
Fragments arranged by size:
2913 929 901 735 336
Bsp1286I G_dGCh'C
Cuts at: 0 95 235 254 428 539 716 743 850
Size: 95 140 19 174 111 177 27 107
Cuts at: 850 1037 1145 1397 1477 1547 1591 1642 1797
Size: 187 108 252 80 70 44 51 155
Cuts at: 1797 1871 2027 2179 2321 2773 2864 2891 3598
Size: 74 156 152 142 452 91 27 707
Cuts at: 3598 3662 3684 3718 3811 3969 3997 4224 4244
Size: 64 22 34 93 158 28 227 20
Cuts at: 4244 4360 4659 4878 5071 5153 5248 5808 5814
Size: 116 299 219 193 82 95 560 6
Fragments arranged by size:
707 560 452 299 252 227 219 193
187 177 174 158 156 155 152 142
140 116 111 108 107 95 95 93
91 82 80 74 70 64 51 44
34 28 27 27 22 20 19 6
BspEI T'CCGG_A
Cuts at: 0 1336 5814
Size: 1336 4478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 866 1845 1961 2098 2528 3317 3533 3704
Size: 866 979 116 137 430 789 216 171
Cuts at: 3704 4233 5297 5546 5814
Size: 529 1064 249 268
Fragments arranged by size:
1064 979 866 789 529 430 268 249
216 171 137 116
BsrBI CCG'CTC
Cuts at: 0 42 1965 2983 3090 4641 5480 5700 5814
Size: 42 1923 1018 107 1551 839 220 114
Fragments arranged by size:
1923 1551 1018 839 220 114 107 42
BsrDI GCAATG_nn'
Cuts at: 0 1734 1943 5814
Size: 1734 209 3871
Fragments arranged by size:
3871 1734 209
BsrFI r'CCGG_y
Cuts at: 0 636 1199 1520 1802 1852 1875 3766 4385
Size: 636 563 321 282 50 23 1891 619
Cuts at: 4385 4589 4816 5405 5814
Size: 204 227 589 409
Fragments arranged by size:
1891 636 619 589 563 409 321 282
227 204 50 23
BsrGI T'GTAC_A
Cuts at: 0 125 5814
Size: 125 5689
BssSI C'ACGA_G
Cuts at: 0 925 3804 4477 4632 5814
Size: 925 2879 673 155 1182
Fragments arranged by size:
2879 1182 925 673 155
BstAPI GCAn_nnn'nTGC
Cuts at: 0 675 5814
Size: 675 5139
BstEII G'GTnAC_C
Cuts at: 0 3081 5814
Size: 3081 2733
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 1225 2079 2163 2920 3015 3648 5028
Size: 243 982 854 84 757 95 633 1380
Cuts at: 5028 5814
Size: 786
Fragments arranged by size:
1380 982 854 786 757 633 243 95
84
BstYI r'GATC_y
Cuts at: 0 1613 1685 2541 4448 5814
Size: 1613 72 856 1907 1366
Fragments arranged by size:
1907 1613 1366 856 72
Bsu36I CC'TnA_GG
Cuts at: 0 2384 2595 2744 3917 5814
Size: 2384 211 149 1173 1897
Fragments arranged by size:
2384 1897 1173 211 149
BtgI C'CryG_G
Cuts at: 0 19 1100 2987 3404 3755 4829 5037 5654
Size: 19 1081 1887 417 351 1074 208 617
Cuts at: 5654 5711 5814
Size: 57 103
Fragments arranged by size:
1887 1081 1074 617 417 351 208 103
57 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1894 4070 5603 5814
Size: 1894 2176 1533 211
Fragments arranged by size:
2176 1894 1533 211
BtsI GCAGTG_nn'
Cuts at: 0 276 617 1031 1125 2055 3140 3196 5814
Size: 276 341 414 94 930 1085 56 2618
Fragments arranged by size:
2618 1085 930 414 341 276 94 56
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1103 1138 4695 4730 5814
Size: 1103 35 3557 35 1084
Fragments arranged by size:
3557 1103 1084 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 2243 4482 5814
Size: 2243 2239 1332
Fragments arranged by size:
2243 2239 1332
EaeI y'GGCC_r
Cuts at: 0 16 245 566 1763 1808 1826 2645 2922
Size: 16 229 321 1197 45 18 819 277
Cuts at: 2922 2984 3768 3848 3906 4192 4320 4814 5325
Size: 62 784 80 58 286 128 494 511
Cuts at: 5325 5552 5708 5814
Size: 227 156 106
Fragments arranged by size:
1197 819 784 511 494 321 286 277
229 227 156 128 106 80 62 58
45 18 16
EagI C'GGCC_G
Cuts at: 0 2984 4192 5814
Size: 2984 1208 1622
Fragments arranged by size:
2984 1622 1208
EarI CTCTTCn'nnn_
Cuts at: 0 722 1096 2905 4040 4786 5275 5814
Size: 722 374 1809 1135 746 489 539
Fragments arranged by size:
1809 1135 746 722 539 489 374
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 685 1539 1792 2820 3471 5659 5814
Size: 685 854 253 1028 651 2188 155
Fragments arranged by size:
2188 1028 854 685 651 253 155
EcoICRI GAG'CTC
Cuts at: 0 714 1143 4358 5814
Size: 714 429 3215 1456
Fragments arranged by size:
3215 1456 714 429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 229 286 469 478 613 719 865
Size: 208 21 57 183 9 135 106 146
Cuts at: 865 874 1300 1579 1588 1832 2032 2296 2396
Size: 9 426 279 9 244 200 264 100
Cuts at: 2396 2818 2872 2886 3408 3685 3884 4552 4597
Size: 422 54 14 522 277 199 668 45
Cuts at: 4597 4974 5140 5814
Size: 377 166 674
Fragments arranged by size:
674 668 522 426 422 377 279 277
264 244 208 200 199 183 166 146
135 106 100 57 54 45 21 14
9 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2200 2337 3916 5542 5814
Size: 2200 137 1579 1626 272
Fragments arranged by size:
2200 1626 1579 272 137
EcoO109I rG'GnC_Cy
Cuts at: 0 618 930 996 1394 1442 1793 1794 1968
Size: 618 312 66 398 48 351 1 174
Cuts at: 1968 2748 3124 3965 3966 3993 3994 4348 4507
Size: 780 376 841 1 27 1 354 159
Cuts at: 4507 4938 5016 5245 5286 5814
Size: 431 78 229 41 528
Fragments arranged by size:
841 780 618 528 431 398 376 354
351 312 229 174 159 78 66 48
41 27 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1305 2652 4485 4815 5814
Size: 1305 1347 1833 330 999
Fragments arranged by size:
1833 1347 1305 999 330
FspI TGC'GCA
Cuts at: 0 1180 3151 5515 5746 5814
Size: 1180 1971 2364 231 68
Fragments arranged by size:
2364 1971 1180 231 68
FspAI rTGC'GCAy
Cuts at: 0 3151 5814
Size: 3151 2663
HaeII r_GCGC'y
Cuts at: 0 337 634 908 1722 4687 5499 5654 5796
Size: 337 297 274 814 2965 812 155 142
Cuts at: 5796 5814
Size: 18
Fragments arranged by size:
2965 814 812 337 297 274 155 142
18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1153 1177 1185 1209 1431 1463 1497 1529
Size: 1153 24 8 24 222 32 34 32
Cuts at: 1529 2662 2694 3402 3434 3524 3556 5440 5472
Size: 1133 32 708 32 90 32 1884 32
Cuts at: 5472 5482 5514 5567 5599 5602 5634 5707 5739
Size: 10 32 53 32 3 32 73 32
Cuts at: 5739 5814
Size: 75
Fragments arranged by size:
1884 1153 1133 708 222 90 75 73
53 34 32 32 32 32 32 32
32 32 32 32 24 24 10 8
3
HincII GTy'rAC
Cuts at: 0 3558 3693 5814
Size: 3558 135 2121
Fragments arranged by size:
3558 2121 135
HindIII A'AGCT_T
Cuts at: 0 4467 5814
Size: 4467 1347
KasI G'GCGC_C
Cuts at: 0 630 5650 5814
Size: 630 5020 164
Fragments arranged by size:
5020 630 164
KpnI G_GTAC'C
Cuts at: 0 2897 5814
Size: 2897 2917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 2120 3248 3521 3665 5814
Size: 806 1314 1128 273 144 2149
Fragments arranged by size:
2149 1314 1128 806 273 144
MscI TGG'CCA
Cuts at: 0 18 1765 1828 2647 2924 3908 5554 5710
Size: 18 1747 63 819 277 984 1646 156
Cuts at: 5710 5814
Size: 104
Fragments arranged by size:
1747 1646 984 819 277 156 104 63
18
MslI CAynn'nnrTG
Cuts at: 0 241 523 1480 1540 3145 3173 3436 3665
Size: 241 282 957 60 1605 28 263 229
Cuts at: 3665 4168 4252 4289 4634 5814
Size: 503 84 37 345 1180
Fragments arranged by size:
1605 1180 957 503 345 282 263 241
229 84 60 37 28
MspA1I CmG'CkG
Cuts at: 0 207 390 684 761 843 1011 1225 1488
Size: 207 183 294 77 82 168 214 263
Cuts at: 1488 1557 2429 2737 3003 3048 3406 3665 4149
Size: 69 872 308 266 45 358 259 484
Cuts at: 4149 4370 4421 4455 4492 4545 4852 5220 5434
Size: 221 51 34 37 53 307 368 214
Cuts at: 5434 5676 5814
Size: 242 138
Fragments arranged by size:
872 484 368 358 308 307 294 266
263 259 242 221 214 214 207 183
168 138 82 77 69 53 51 45
37 34
NaeI GCC'GGC
Cuts at: 0 1201 1522 1854 1877 3768 4818 5814
Size: 1201 321 332 23 1891 1050 996
Fragments arranged by size:
1891 1201 1050 996 332 321 23
NarI GG'CG_CC
Cuts at: 0 631 5651 5814
Size: 631 5020 163
Fragments arranged by size:
5020 631 163
NcoI C'CATG_G
Cuts at: 0 19 1100 5037 5814
Size: 19 1081 3937 777
Fragments arranged by size:
3937 1081 777 19
NgoMIV G'CCGG_C
Cuts at: 0 1199 1520 1852 1875 3766 4816 5814
Size: 1199 321 332 23 1891 1050 998
Fragments arranged by size:
1891 1199 1050 998 332 321 23
NsiI A_TGCA'T
Cuts at: 0 373 5814
Size: 373 5441
NspI r_CATG'y
Cuts at: 0 3135 4671 4693 5745 5814
Size: 3135 1536 22 1052 69
Fragments arranged by size:
3135 1536 1052 69 22
PasI CC'CwG_GG
Cuts at: 0 254 1973 4837 5814
Size: 254 1719 2864 977
Fragments arranged by size:
2864 1719 977 254
PciI A'CATG_T
Cuts at: 0 3131 5814
Size: 3131 2683
PflMI CCAn_nnn'nTGG
Cuts at: 0 2208 2935 3231 3267 4530 4948 5814
Size: 2208 727 296 36 1263 418 866
Fragments arranged by size:
2208 1263 866 727 418 296 36
PfoI T'CCnGG_A
Cuts at: 0 512 1250 3860 5282 5814
Size: 512 738 2610 1422 532
Fragments arranged by size:
2610 1422 738 532 512
PmlI CAC'GTG
Cuts at: 0 3657 5814
Size: 3657 2157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2014 2046 5814
Size: 2014 32 3768
Fragments arranged by size:
3768 2014 32
PpuMI rG'GwC_Cy
Cuts at: 0 618 996 1968 3124 4348 5016 5286 5814
Size: 618 378 972 1156 1224 668 270 528
Fragments arranged by size:
1224 1156 972 668 618 528 378 270
PshAI GACnn'nnGTC
Cuts at: 0 4545 5814
Size: 4545 1269
PspOMI G'GGCC_C
Cuts at: 0 1393 1793 3965 3993 5244 5814
Size: 1393 400 2172 28 1251 570
Fragments arranged by size:
2172 1393 1251 570 400 28
PspXI vC'TCGA_Gb
Cuts at: 0 4871 5814
Size: 4871 943
PstI C_TGCA'G
Cuts at: 0 389 683 1016 2434 2541 5201 5348 5489
Size: 389 294 333 1418 107 2660 147 141
Cuts at: 5489 5814
Size: 325
Fragments arranged by size:
2660 1418 389 333 325 294 147 141
107
PvuI CG_AT'CG
Cuts at: 0 4199 5814
Size: 4199 1615
PvuII CAG'CTG
Cuts at: 0 390 684 761 843 1011 1225 1488 1557
Size: 390 294 77 82 168 214 263 69
Cuts at: 1557 3048 3665 4149 4455 5220 5434 5676 5814
Size: 1491 617 484 306 765 214 242 138
Fragments arranged by size:
1491 765 617 484 390 306 294 263
242 214 214 168 138 82 77 69
SacI G_AGCT'C
Cuts at: 0 716 1145 4360 5814
Size: 716 429 3215 1454
Fragments arranged by size:
3215 1454 716 429
SacII CC_GC'GG
Cuts at: 0 3407 5814
Size: 3407 2407
SanDI GG'GwC_CC
Cuts at: 0 618 1968 3124 5814
Size: 618 1350 1156 2690
Fragments arranged by size:
2690 1350 1156 618
SapI GCTCTTCn'nnn_
Cuts at: 0 722 5275 5814
Size: 722 4553 539
Fragments arranged by size:
4553 722 539
SbfI CC_TGCA'GG
Cuts at: 0 5489 5814
Size: 5489 325
ScaI AGT'ACT
Cuts at: 0 598 2191 2917 5814
Size: 598 1593 726 2897
Fragments arranged by size:
2897 1593 726 598
SexAI A'CCwGG_T
Cuts at: 0 5060 5814
Size: 5060 754
SfcI C'TryA_G
Cuts at: 0 385 679 1012 1452 2430 2537 3366 5197
Size: 385 294 333 440 978 107 829 1831
Cuts at: 5197 5344 5485 5814
Size: 147 141 329
Fragments arranged by size:
1831 978 829 440 385 333 329 294
147 141 107
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3110 5716 5814
Size: 3110 2606 98
Fragments arranged by size:
3110 2606 98
SfoI GGC'GCC
Cuts at: 0 632 5652 5814
Size: 632 5020 162
Fragments arranged by size:
5020 632 162
SgrAI Cr'CCGG_yG
Cuts at: 0 636 5814
Size: 636 5178
SmaI CCC'GGG
Cuts at: 0 1585 1918 5814
Size: 1585 333 3896
Fragments arranged by size:
3896 1585 333
SmlI C'TyrA_G
Cuts at: 0 4789 4871 5785 5814
Size: 4789 82 914 29
Fragments arranged by size:
4789 914 82 29
SnaBI TAC'GTA
Cuts at: 0 3708 5814
Size: 3708 2106
SpeI A'CTAG_T
Cuts at: 0 4804 5814
Size: 4804 1010
SphI G_CATG'C
Cuts at: 0 5745 5814
Size: 5745 69
StuI AGG'CCT
Cuts at: 0 808 1648 5560 5814
Size: 808 840 3912 254
Fragments arranged by size:
3912 840 808 254
StyI C'CwwG_G
Cuts at: 0 19 1100 1289 1385 2828 5037 5274 5555
Size: 19 1081 189 96 1443 2209 237 281
Cuts at: 5555 5814
Size: 259
Fragments arranged by size:
2209 1443 1081 281 259 237 189 96
19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1235 2089 3100 4702 5146 5814
Size: 1235 854 1011 1602 444 668
Fragments arranged by size:
1602 1235 1011 854 668 444
TatI w'GTAC_w
Cuts at: 0 125 596 1604 2189 2915 5312 5814
Size: 125 471 1008 585 726 2397 502
Fragments arranged by size:
2397 1008 726 585 502 471 125
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1561 1593 3121 3153 3334 3365 3366 3397
Size: 1561 32 1528 32 181 31 1 31
Cuts at: 3397 4945 4977 5278 5310 5814
Size: 1548 32 301 32 504
Fragments arranged by size:
1561 1548 1528 504 301 181 32 32
32 32 31 31 1
Tth111I GACn'n_nGTC
Cuts at: 0 520 5814
Size: 520 5294
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 828 1248 2932 3901 4251 4527 4707 4890
Size: 828 420 1684 969 350 276 180 183
Cuts at: 4890 5610 5814
Size: 720 204
Fragments arranged by size:
1684 969 828 720 420 350 276 204
183 180
XhoI C'TCGA_G
Cuts at: 0 4871 5814
Size: 4871 943
XmaI C'CCGG_G
Cuts at: 0 1583 1916 5814
Size: 1583 333 3898
Fragments arranged by size:
3898 1583 333
XmnI GAAnn'nnTTC
Cuts at: 0 3184 5814
Size: 3184 2630
ZraI GAC'GTC
Cuts at: 0 1322 5814
Size: 1322 4492
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI FspAI HaeII Hin4I HincII HindIII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI
PvuII SacI SacII SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII ApoI AscI AseI AsiSI AvrII BaeI
BamHI BclI BmtI BsiWI BspHI BssHII BstBI BstZ17I
ClaI DraI DrdI EcoRI EcoRV FseI HpaI MfeI
MluI NdeI NheI NotI NruI PacI PmeI PsiI
PsrI RsrII SalI SgfI SrfI SspI SwaI XbaI