Restriction map for
pF1KA0405
(Linear) (Six-base) MAPSORT of: KIAA0405.seq Check: 3162 from: 1 to: 1980
KIAA0405.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AatII G_ACGT'C
Cuts at: 0 1600 1980
Size: 1600 380
AccI GT'mk_AC
Cuts at: 0 140 278 1980
Size: 140 138 1702
Fragments arranged by size:
1702 140 138
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 660 925 1980
Size: 67 593 265 1055
Fragments arranged by size:
1055 593 265 67
AleI CACnn'nnGTG
Cuts at: 0 1360 1980
Size: 1360 620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 932 1980
Size: 932 1048
ApaLI G'TGCA_C
Cuts at: 0 268 1980
Size: 268 1712
ApoI r'AATT_y
Cuts at: 0 304 700 1980
Size: 304 396 1280
Fragments arranged by size:
1280 396 304
AseI AT'TA_AT
Cuts at: 0 224 1901 1980
Size: 224 1677 79
Fragments arranged by size:
1677 224 79
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 642 675 1980
Size: 642 33 1305
Fragments arranged by size:
1305 642 33
BamHI G'GATC_C
Cuts at: 0 179 1271 1980
Size: 179 1092 709
Fragments arranged by size:
1092 709 179
BanI G'GyrC_C
Cuts at: 0 670 1774 1980
Size: 670 1104 206
Fragments arranged by size:
1104 670 206
BanII G_rGCy'C
Cuts at: 0 77 1454 1615 1980
Size: 77 1377 161 365
Fragments arranged by size:
1377 365 161 77
BbsI GAAGACnn'nnnn_
Cuts at: 0 458 1520 1980
Size: 458 1062 460
Fragments arranged by size:
1062 460 458
BclI T'GATC_A
Cuts at: 0 761 1980
Size: 761 1219
BglI GCCn_nnn'nGGC
Cuts at: 0 528 1980
Size: 528 1452
BlpI GC'TnA_GC
Cuts at: 0 1675 1980
Size: 1675 305
Bme1580I G_kGCm'C
Cuts at: 0 272 673 1980
Size: 272 401 1307
Fragments arranged by size:
1307 401 272
BmrI ACTGGGnnnn_n'
Cuts at: 0 1239 1980
Size: 1239 741
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1819 1980
Size: 1819 161
Bpu10I CC'TnA_GC
Cuts at: 0 511 1432 1980
Size: 511 921 548
Fragments arranged by size:
921 548 511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 860 1980
Size: 679 181 1120
Fragments arranged by size:
1120 679 181
BsaAI yAC'GTr
Cuts at: 0 715 1980
Size: 715 1265
BsaBI GATnn'nnATC
Cuts at: 0 566 1980
Size: 566 1414
BsaHI Gr'CG_yC
Cuts at: 0 1597 1980
Size: 1597 383
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 191 221 1116 1131 1146 1161 1980
Size: 191 30 895 15 15 15 819
Fragments arranged by size:
895 819 191 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 725 1980
Size: 725 1255
BseYI C'CCAG_C
Cuts at: 0 25 389 1113 1980
Size: 25 364 724 867
Fragments arranged by size:
867 724 364 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 397 1980
Size: 235 162 1583
Fragments arranged by size:
1583 235 162
BsiHKAI G_wGCw'C
Cuts at: 0 272 1970 1980
Size: 272 1698 10
Fragments arranged by size:
1698 272 10
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1833 1980
Size: 1833 147
Bsp1286I G_dGCh'C
Cuts at: 0 77 272 673 1454 1615 1970 1980
Size: 77 195 401 781 161 355 10
Fragments arranged by size:
781 401 355 195 161 77 10
BsrFI r'CCGG_y
Cuts at: 0 1745 1980
Size: 1745 235
BsrGI T'GTAC_A
Cuts at: 0 258 1980
Size: 258 1722
BssSI C'ACGA_G
Cuts at: 0 609 1980
Size: 609 1371
BstXI CCAn_nnnn'nTGG
Cuts at: 0 99 1980
Size: 99 1881
BstYI r'GATC_y
Cuts at: 0 179 1271 1980
Size: 179 1092 709
Fragments arranged by size:
1092 709 179
BtgI C'CryG_G
Cuts at: 0 30 1980
Size: 30 1950
BtsI GCAGTG_nn'
Cuts at: 0 522 914 1968 1980
Size: 522 392 1054 12
Fragments arranged by size:
1054 522 392 12
DraIII CAC_nnn'GTG
Cuts at: 0 20 1980
Size: 20 1960
EaeI y'GGCC_r
Cuts at: 0 1043 1980
Size: 1043 937
EarI CTCTTCn'nnn_
Cuts at: 0 401 1111 1336 1980
Size: 401 710 225 644
Fragments arranged by size:
710 644 401 225
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1764 1980
Size: 1764 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 660 925 1819 1980
Size: 67 593 265 894 161
Fragments arranged by size:
894 593 265 161 67
EcoO109I rG'GnC_Cy
Cuts at: 0 458 1021 1980
Size: 458 563 959
Fragments arranged by size:
959 563 458
EcoRI G'AATT_C
Cuts at: 0 304 1980
Size: 304 1676
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1145 1980
Size: 1145 835
HaeII r_GCGC'y
Cuts at: 0 1713 1980
Size: 1713 267
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 412 444 858 880 890 912 1441 1473
Size: 412 32 414 22 10 22 529 32
Cuts at: 1473 1980
Size: 507
Fragments arranged by size:
529 507 414 412 32 32 22 22
10
HincII GTy'rAC
Cuts at: 0 1443 1980
Size: 1443 537
HindIII A'AGCT_T
Cuts at: 0 156 400 1980
Size: 156 244 1580
Fragments arranged by size:
1580 244 156
HpaI GTT'AAC
Cuts at: 0 1443 1980
Size: 1443 537
MfeI C'AATT_G
Cuts at: 0 707 1980
Size: 707 1273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 875 923 1159 1216 1980
Size: 611 264 48 236 57 764
Fragments arranged by size:
764 611 264 236 57 48
MslI CAynn'nnrTG
Cuts at: 0 419 1339 1360 1980
Size: 419 920 21 620
Fragments arranged by size:
920 620 419 21
MspA1I CmG'CkG
Cuts at: 0 122 1319 1415 1980
Size: 122 1197 96 565
Fragments arranged by size:
1197 565 122 96
NaeI GCC'GGC
Cuts at: 0 1747 1980
Size: 1747 233
NcoI C'CATG_G
Cuts at: 0 30 1980
Size: 30 1950
NdeI CA'TA_TG
Cuts at: 0 1694 1980
Size: 1694 286
NgoMIV G'CCGG_C
Cuts at: 0 1745 1980
Size: 1745 235
NspI r_CATG'y
Cuts at: 0 1935 1980
Size: 1935 45
PasI CC'CwG_GG
Cuts at: 0 69 1980
Size: 69 1911
PflMI CCAn_nnn'nTGG
Cuts at: 0 20 1608 1980
Size: 20 1588 372
Fragments arranged by size:
1588 372 20
PfoI T'CCnGG_A
Cuts at: 0 182 464 1796 1980
Size: 182 282 1332 184
Fragments arranged by size:
1332 282 184 182
PpuMI rG'GwC_Cy
Cuts at: 0 1021 1980
Size: 1021 959
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 124 156 1980
Size: 124 32 1824
Fragments arranged by size:
1824 124 32
PstI C_TGCA'G
Cuts at: 0 246 949 1874 1980
Size: 246 703 925 106
Fragments arranged by size:
925 703 246 106
PvuII CAG'CTG
Cuts at: 0 1319 1980
Size: 1319 661
SapI GCTCTTCn'nnn_
Cuts at: 0 401 1980
Size: 401 1579
SfcI C'TryA_G
Cuts at: 0 7 242 945 1870 1980
Size: 7 235 703 925 110
Fragments arranged by size:
925 703 235 110 7
SmlI C'TyrA_G
Cuts at: 0 694 875 1980
Size: 694 181 1105
Fragments arranged by size:
1105 694 181
SnaBI TAC'GTA
Cuts at: 0 715 1980
Size: 715 1265
SspI AAT'ATT
Cuts at: 0 360 1980
Size: 360 1620
StyI C'CwwG_G
Cuts at: 0 30 931 1017 1980
Size: 30 901 86 963
Fragments arranged by size:
963 901 86 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1350 1555 1980
Size: 1350 205 425
Fragments arranged by size:
1350 425 205
TatI w'GTAC_w
Cuts at: 0 258 1123 1980
Size: 258 865 857
Fragments arranged by size:
865 857 258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1131 1163 1980
Size: 1131 32 817
Fragments arranged by size:
1131 817 32
Tth111I GACn'n_nGTC
Cuts at: 0 1596 1980
Size: 1596 384
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 438 444 1980
Size: 438 6 1536
Fragments arranged by size:
1536 438 6
ZraI GAC'GTC
Cuts at: 0 1598 1980
Size: 1598 382
Enzymes that do cut:
AatII AccI AcuI AleI AlfI ApaLI ApoI AseI
BaeI BamHI BanI BanII BbsI BclI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrFI
BsrGI BssSI BstXI BstYI BtgI BtsI DraIII EaeI
EarI EciI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII HpaI MfeI MmeI MslI MspA1I NaeI
NcoI NdeI NgoMIV NspI PasI PflMI PfoI PpuMI
PsrI PstI PvuII SapI SfcI SmlI SnaBI SspI
StyI TaqII TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaI AscI AsiSI AvaI AvrII BbeI
BbvCI BcgI BciVI BfrBI BglII BmgBI BmtI BplI
BsaI BsaWI BsiEI BsiWI BsmI BspEI BspHI BspMI
BsrBI BsrDI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoNI
EcoRV FseI FspI FspAI KasI KpnI MluI MscI
NarI NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SpeI SphI SrfI StuI
SwaI XbaI XhoI XmaI XmnI