Restriction map for
pF1KA0407
(Linear) (Six-base) MAPSORT of: KIAA0407.seq Check: 8713 from: 1 to: 6405
KIAA0407.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1508 2947 4026 6405
Size: 1508 1439 1079 2379
Fragments arranged by size:
2379 1508 1439 1079
AccI GT'mk_AC
Cuts at: 0 1292 3245 3742 5086 6405
Size: 1292 1953 497 1344 1319
Fragments arranged by size:
1953 1344 1319 1292 497
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1444 2316 6405
Size: 1444 872 4089
Fragments arranged by size:
4089 1444 872
AhdI GACnn_n'nnGTC
Cuts at: 0 3451 6175 6405
Size: 3451 2724 230
Fragments arranged by size:
3451 2724 230
AleI CACnn'nnGTG
Cuts at: 0 1683 3424 6405
Size: 1683 1741 2981
Fragments arranged by size:
2981 1741 1683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 118 1367 2751 2833 6405
Size: 118 1249 1384 82 3572
Fragments arranged by size:
3572 1384 1249 118 82
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5791 5823 6405
Size: 5791 32 582
Fragments arranged by size:
5791 582 32
AlwNI CAG_nnn'CTG
Cuts at: 0 111 233 390 1455 1943 1974 2204 2224
Size: 111 122 157 1065 488 31 230 20
Cuts at: 2224 2413 3092 3828 4332 4554 5041 5484 6379
Size: 189 679 736 504 222 487 443 895
Cuts at: 6379 6405
Size: 26
Fragments arranged by size:
1065 895 736 679 504 488 487 443
230 222 189 157 122 111 31 26
20
ApaI G_GGCC'C
Cuts at: 0 16 321 564 1303 1558 2009 2641 6405
Size: 16 305 243 739 255 451 632 3764
Fragments arranged by size:
3764 739 632 451 305 255 243 16
ApaLI G'TGCA_C
Cuts at: 0 340 1091 1977 6405
Size: 340 751 886 4428
Fragments arranged by size:
4428 886 751 340
ApoI r'AATT_y
Cuts at: 0 4060 4627 6405
Size: 4060 567 1778
Fragments arranged by size:
4060 1778 567
AvaI C'yCGr_G
Cuts at: 0 379 557 746 1025 1542 2419 2548 2692
Size: 379 178 189 279 517 877 129 144
Cuts at: 2692 2875 2894 3158 3870 4892 6244 6405
Size: 183 19 264 712 1022 1352 161
Fragments arranged by size:
1352 1022 877 712 517 379 279 264
189 183 178 161 144 129 19
BamHI G'GATC_C
Cuts at: 0 3480 6405
Size: 3480 2925
BanI G'GyrC_C
Cuts at: 0 133 365 3253 3415 3432 3529 4295 4340
Size: 133 232 2888 162 17 97 766 45
Cuts at: 4340 4359 4486 4736 5438 5492 5651 5779 6405
Size: 19 127 250 702 54 159 128 626
Fragments arranged by size:
2888 766 702 626 250 232 162 159
133 128 127 97 54 45 19 17
BanII G_rGCy'C
Cuts at: 0 16 178 313 321 491 564 1303 1558
Size: 16 162 135 8 170 73 739 255
Cuts at: 1558 1835 2009 2032 2145 2276 2500 2641 2699
Size: 277 174 23 113 131 224 141 58
Cuts at: 2699 2764 2984 3277 4316 4444 6405
Size: 65 220 293 1039 128 1961
Fragments arranged by size:
1961 1039 739 293 277 255 224 220
174 170 162 141 135 131 128 113
73 65 58 23 16 8
BbeI G_GCGC'C
Cuts at: 0 369 3419 5496 6405
Size: 369 3050 2077 909
Fragments arranged by size:
3050 2077 909 369
BbsI GAAGACnn'nnnn_
Cuts at: 0 4288 5935 6405
Size: 4288 1647 470
Fragments arranged by size:
4288 1647 470
BbvCI CC'TCA_GC
Cuts at: 0 1040 2340 3035 3179 6405
Size: 1040 1300 695 144 3226
Fragments arranged by size:
3226 1300 1040 695 144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 279 313 998 1032 3861 3895 6405
Size: 279 34 685 34 2829 34 2510
Fragments arranged by size:
2829 2510 685 279 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 505 1827 6405
Size: 505 1322 4578
Fragments arranged by size:
4578 1322 505
BglI GCCn_nnn'nGGC
Cuts at: 0 405 616 4176 4909 5688 5711 6405
Size: 405 211 3560 733 779 23 694
Fragments arranged by size:
3560 779 733 694 405 211 23
BglII A'GATC_T
Cuts at: 0 5747 6399 6405
Size: 5747 652 6
Fragments arranged by size:
5747 652 6
BlpI GC'TnA_GC
Cuts at: 0 172 1448 2197 2956 6405
Size: 172 1276 749 759 3449
Fragments arranged by size:
3449 1276 759 749 172
Bme1580I G_kGCm'C
Cuts at: 0 16 265 321 344 564 1095 1303 1558
Size: 16 249 56 23 220 531 208 255
Cuts at: 1558 1765 1981 2009 2402 2444 2641 3202 3335
Size: 207 216 28 393 42 197 561 133
Cuts at: 3335 3532 4489 4739 5303 5441 5782 6405
Size: 197 957 250 564 138 341 623
Fragments arranged by size:
957 623 564 561 531 393 341 255
250 249 220 216 208 207 197 197
138 133 56 42 28 23 16
BmgBI CAC'GTC
Cuts at: 0 848 3448 3802 3834 5994 6405
Size: 848 2600 354 32 2160 411
Fragments arranged by size:
2600 2160 848 411 354 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 1783 2919 3105 3189 3245 3575 4989 6405
Size: 1783 1136 186 84 56 330 1414 1416
Fragments arranged by size:
1783 1416 1414 1136 330 186 84 56
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 772 2292 5395 6219 6405
Size: 772 1520 3103 824 186
Fragments arranged by size:
3103 1520 824 772 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 43 211 968 1213 2887 2900 3347
Size: 10 33 168 757 245 1674 13 447
Cuts at: 3347 3351 3595 4606 4615 4888 5618 5638 6337
Size: 4 244 1011 9 273 730 20 699
Cuts at: 6337 6346 6405
Size: 9 59
Fragments arranged by size:
1674 1011 757 730 699 447 273 245
244 168 59 33 20 13 10 9
9 4
Bpu10I CC'TnA_GC
Cuts at: 0 1040 1586 2340 3035 3179 6405
Size: 1040 546 754 695 144 3226
Fragments arranged by size:
3226 1040 754 695 546 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2653 4966 6405
Size: 2653 2313 1439
Fragments arranged by size:
2653 2313 1439
BsaI GGTCTCn'nnnn_
Cuts at: 0 1358 3369 3593 3646 3873 6233 6405
Size: 1358 2011 224 53 227 2360 172
Fragments arranged by size:
2360 2011 1358 227 224 172 53
BsaAI yAC'GTr
Cuts at: 0 98 670 1815 4007 4422 4908 6405
Size: 98 572 1145 2192 415 486 1497
Fragments arranged by size:
2192 1497 1145 572 486 415 98
BsaBI GATnn'nnATC
Cuts at: 0 1121 5074 6405
Size: 1121 3953 1331
Fragments arranged by size:
3953 1331 1121
BsaHI Gr'CG_yC
Cuts at: 0 366 3416 3844 3909 5493 6053 6405
Size: 366 3050 428 65 1584 560 352
Fragments arranged by size:
3050 1584 560 428 366 352 65
BsaWI w'CCGG_w
Cuts at: 0 6235 6405
Size: 6235 170
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 760 790 1309 1339 2097 2127 3349 3379
Size: 760 30 519 30 758 30 1222 30
Cuts at: 3379 3616 3646 4007 4037 5104 5134 5314 5344
Size: 237 30 361 30 1067 30 180 30
Cuts at: 5344 5522 5552 6405
Size: 178 30 853
Fragments arranged by size:
1222 1067 853 760 758 519 361 237
180 178 30 30 30 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 616 882 1654 1817 2284 2593 3418 3981
Size: 616 266 772 163 467 309 825 563
Cuts at: 3981 3985 4255 6334 6405
Size: 4 270 2079 71
Fragments arranged by size:
2079 825 772 616 563 467 309 270
266 163 71 4
BseYI C'CCAG_C
Cuts at: 0 15 272 937 1147 1590 1943 2002 2068
Size: 15 257 665 210 443 353 59 66
Cuts at: 2068 2284 3201 3306 3523 3886 3995 4806 5384
Size: 216 917 105 217 363 109 811 578
Cuts at: 5384 5885 6078 6180 6348 6405
Size: 501 193 102 168 57
Fragments arranged by size:
917 811 665 578 501 443 363 353
257 217 216 210 193 168 109 105
102 66 59 57 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 884 1267 1542 2803 3316 4408 4444 6042
Size: 884 383 275 1261 513 1092 36 1598
Cuts at: 6042 6405
Size: 363
Fragments arranged by size:
1598 1261 1092 884 513 383 363 275
36
BsiEI CG_ry'CG
Cuts at: 0 3015 6405
Size: 3015 3390
BsiHKAI G_wGCw'C
Cuts at: 0 344 878 1095 1394 1417 1449 1511 1543
Size: 344 534 217 299 23 32 62 32
Cuts at: 1543 1835 1981 2984 5573 6026 6405
Size: 292 146 1003 2589 453 379
Fragments arranged by size:
2589 1003 534 453 379 344 299 292
217 146 62 32 32 23
BsmI GAATG_Cn'
Cuts at: 0 79 539 3881 5259 6405
Size: 79 460 3342 1378 1146
Fragments arranged by size:
3342 1378 1146 460 79
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1636 3541 3919 6405
Size: 1636 1905 378 2486
Fragments arranged by size:
2486 1905 1636 378
Bsp1286I G_dGCh'C
Cuts at: 0 16 178 265 313 321 344 491 564
Size: 16 162 87 48 8 23 147 73
Cuts at: 564 878 1095 1303 1394 1417 1449 1511 1543
Size: 314 217 208 91 23 32 62 32
Cuts at: 1543 1558 1765 1835 1981 2009 2032 2145 2276
Size: 15 207 70 146 28 23 113 131
Cuts at: 2276 2402 2444 2500 2641 2699 2764 2984 3202
Size: 126 42 56 141 58 65 220 218
Cuts at: 3202 3277 3335 3532 4316 4444 4489 4739 5303
Size: 75 58 197 784 128 45 250 564
Cuts at: 5303 5441 5573 5782 6026 6405
Size: 138 132 209 244 379
Fragments arranged by size:
784 564 379 314 250 244 220 218
217 209 208 207 197 162 147 146
141 138 132 131 128 126 113 91
87 75 73 70 65 62 58 58
56 48 45 42 32 32 28 23
23 23 16 15 8
BspEI T'CCGG_A
Cuts at: 0 6235 6405
Size: 6235 170
BspHI T'CATG_A
Cuts at: 0 1403 4640 6405
Size: 1403 3237 1765
Fragments arranged by size:
3237 1765 1403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 716 1508 1682 2209 2459 2825 2947 4026
Size: 716 792 174 527 250 366 122 1079
Cuts at: 4026 4832 5876 6405
Size: 806 1044 529
Fragments arranged by size:
1079 1044 806 792 716 529 527 366
250 174 122
BsrBI CCG'CTC
Cuts at: 0 405 2790 3714 5604 5844 6405
Size: 405 2385 924 1890 240 561
Fragments arranged by size:
2385 1890 924 561 405 240
BsrDI GCAATG_nn'
Cuts at: 0 4235 6032 6405
Size: 4235 1797 373
Fragments arranged by size:
4235 1797 373
BsrFI r'CCGG_y
Cuts at: 0 208 917 989 2775 3010 3412 6405
Size: 208 709 72 1786 235 402 2993
Fragments arranged by size:
2993 1786 709 402 235 208 72
BsrGI T'GTAC_A
Cuts at: 0 4529 5120 6405
Size: 4529 591 1285
Fragments arranged by size:
4529 1285 591
BssHII G'CGCG_C
Cuts at: 0 5727 6405
Size: 5727 678
BssSI C'ACGA_G
Cuts at: 0 1992 2526 3376 6405
Size: 1992 534 850 3029
Fragments arranged by size:
3029 1992 850 534
BstAPI GCAn_nnn'nTGC
Cuts at: 0 233 390 3092 3303 4332 6405
Size: 233 157 2702 211 1029 2073
Fragments arranged by size:
2702 2073 1029 233 211 157
BstEII G'GTnAC_C
Cuts at: 0 3858 6405
Size: 3858 2547
BstXI CCAn_nnnn'nTGG
Cuts at: 0 858 1155 1565 1685 2100 3426 3898 5893
Size: 858 297 410 120 415 1326 472 1995
Cuts at: 5893 6405
Size: 512
Fragments arranged by size:
1995 1326 858 512 472 415 410 297
120
BstYI r'GATC_y
Cuts at: 0 3480 4577 4721 5747 6399 6405
Size: 3480 1097 144 1026 652 6
Fragments arranged by size:
3480 1097 1026 652 144 6
BstZ17I GTA'TAC
Cuts at: 0 3743 5087 6405
Size: 3743 1344 1318
Fragments arranged by size:
3743 1344 1318
Bsu36I CC'TnA_GG
Cuts at: 0 129 2351 4037 4154 4555 6405
Size: 129 2222 1686 117 401 1850
Fragments arranged by size:
2222 1850 1686 401 129 117
BtgI C'CryG_G
Cuts at: 0 1154 1232 2009 2129 2212 2492 3068 3510
Size: 1154 78 777 120 83 280 576 442
Cuts at: 3510 3707 3816 3863 4202 4319 4336 4925 6405
Size: 197 109 47 339 117 17 589 1480
Fragments arranged by size:
1480 1154 777 589 576 442 339 280
197 120 117 109 83 78 47 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1236 1325 6405
Size: 1236 89 5080
Fragments arranged by size:
5080 1236 89
BtsI GCAGTG_nn'
Cuts at: 0 626 1361 1570 1924 2402 4608 4930 5503
Size: 626 735 209 354 478 2206 322 573
Cuts at: 5503 6308 6405
Size: 805 97
Fragments arranged by size:
2206 805 735 626 573 478 354 322
209 97
DraIII CAC_nnn'GTG
Cuts at: 0 654 3151 3197 3222 5151 5658 6405
Size: 654 2497 46 25 1929 507 747
Fragments arranged by size:
2497 1929 747 654 507 46 25
DrdI GACnn_nn'nnGTC
Cuts at: 0 3908 4048 4078 5461 6405
Size: 3908 140 30 1383 944
Fragments arranged by size:
3908 1383 944 140 30
EaeI y'GGCC_r
Cuts at: 0 397 568 810 842 1157 1198 1314 1687
Size: 397 171 242 32 315 41 116 373
Cuts at: 1687 2102 3012 3188 3410 3819 5003 5389 5432
Size: 415 910 176 222 409 1184 386 43
Cuts at: 5432 6065 6405
Size: 633 340
Fragments arranged by size:
1184 910 633 415 409 397 386 373
340 315 242 222 176 171 116 43
41 32
EagI C'GGCC_G
Cuts at: 0 3012 6405
Size: 3012 3393
EarI CTCTTCn'nnn_
Cuts at: 0 26 2432 2705 3435 4499 4534 4846 6405
Size: 26 2406 273 730 1064 35 312 1559
Fragments arranged by size:
2406 1559 1064 730 312 273 35 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1894 2569 6405
Size: 1894 675 3836
Fragments arranged by size:
3836 1894 675
EcoICRI GAG'CTC
Cuts at: 0 1833 2982 6405
Size: 1833 1149 3423
Fragments arranged by size:
3423 1833 1149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 43 211 968 1213 1444 2316 2887
Size: 10 33 168 757 245 231 872 571
Cuts at: 2887 2900 3347 3351 3595 4606 4615 4888 5618
Size: 13 447 4 244 1011 9 273 730
Cuts at: 5618 5638 6337 6346 6405
Size: 20 699 9 59
Fragments arranged by size:
1011 872 757 730 699 571 447 273
245 244 231 168 59 33 20 13
10 9 9 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 979 2057 2827 4036 4354 5094 5279 5809
Size: 979 1078 770 1209 318 740 185 530
Cuts at: 5809 6319 6405
Size: 510 86
Fragments arranged by size:
1209 1078 979 770 740 530 510 318
185 86
EcoO109I rG'GnC_Cy
Cuts at: 0 48 120 317 318 561 705 1167 1382
Size: 48 72 197 1 243 144 462 215
Cuts at: 1382 1485 1554 1785 2355 2637 2638 2871 4041
Size: 103 69 231 570 282 1 233 1170
Cuts at: 4041 4158 4551 4761 5272 5310 5520 6405
Size: 117 393 210 511 38 210 885
Fragments arranged by size:
1170 885 570 511 462 393 282 243
233 231 215 210 210 197 144 117
103 72 69 48 38 1 1
EcoRI G'AATT_C
Cuts at: 0 4627 6405
Size: 4627 1778
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3762 5877 6405
Size: 3762 2115 528
Fragments arranged by size:
3762 2115 528
FseI GG_CCGG'CC
Cuts at: 0 921 3014 6405
Size: 921 2093 3391
Fragments arranged by size:
3391 2093 921
FspI TGC'GCA
Cuts at: 0 1916 5029 6405
Size: 1916 3113 1376
Fragments arranged by size:
3113 1916 1376
HaeII r_GCGC'y
Cuts at: 0 369 683 1847 3208 3419 5496 6405
Size: 369 314 1164 1361 211 2077 909
Fragments arranged by size:
2077 1361 1164 909 369 314 211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 1235 1267 1450 1482 1801 1833
Size: 112 32 1091 32 183 32 319 32
Cuts at: 1833 2605 2637 3437 3469 3616 3648 4641 4673
Size: 772 32 800 32 147 32 993 32
Cuts at: 4673 5314 5346 5407 5439 6161 6193 6405
Size: 641 32 61 32 722 32 212
Fragments arranged by size:
1091 993 800 772 722 641 319 212
183 147 112 61 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3987 6405
Size: 3987 2418
HindIII A'AGCT_T
Cuts at: 0 4942 6405
Size: 4942 1463
KasI G'GCGC_C
Cuts at: 0 365 3415 5492 6405
Size: 365 3050 2077 913
Fragments arranged by size:
3050 2077 913 365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3302 3576 5927 6405
Size: 3302 274 2351 478
Fragments arranged by size:
3302 2351 478 274
MscI TGG'CCA
Cuts at: 0 844 1159 1200 1316 1689 2104 3190 3821
Size: 844 315 41 116 373 415 1086 631
Cuts at: 3821 5005 6405
Size: 1184 1400
Fragments arranged by size:
1400 1184 1086 844 631 415 373 315
116 41
MslI CAynn'nnrTG
Cuts at: 0 1397 1683 2770 3424 3586 6405
Size: 1397 286 1087 654 162 2819
Fragments arranged by size:
2819 1397 1087 654 286 162
MspA1I CmG'CkG
Cuts at: 0 171 189 297 363 387 838 1098 1173
Size: 171 18 108 66 24 451 260 75
Cuts at: 1173 1219 1281 1943 2311 2373 3092 3424 3512
Size: 46 62 662 368 62 719 332 88
Cuts at: 3512 4584 4616 4682 5388 6348 6376 6405
Size: 1072 32 66 706 960 28 29
Fragments arranged by size:
1072 960 719 706 662 451 368 332
260 171 108 88 75 66 66 62
62 46 32 29 28 24 18
NaeI GCC'GGC
Cuts at: 0 919 3012 3414 6405
Size: 919 2093 402 2991
Fragments arranged by size:
2991 2093 919 402
NarI GG'CG_CC
Cuts at: 0 366 3416 5493 6405
Size: 366 3050 2077 912
Fragments arranged by size:
3050 2077 912 366
NcoI C'CATG_G
Cuts at: 0 1154 1232 2009 2212 2492 6405
Size: 1154 78 777 203 280 3913
Fragments arranged by size:
3913 1154 777 280 203 78
NgoMIV G'CCGG_C
Cuts at: 0 917 3010 3412 6405
Size: 917 2093 402 2993
Fragments arranged by size:
2993 2093 917 402
NspI r_CATG'y
Cuts at: 0 3049 3295 3935 5127 5569 6405
Size: 3049 246 640 1192 442 836
Fragments arranged by size:
3049 1192 836 640 442 246
PasI CC'CwG_GG
Cuts at: 0 178 313 471 1303 2204 2485 4046 4413
Size: 178 135 158 832 901 281 1561 367
Cuts at: 4413 4444 4732 5292 6405
Size: 31 288 560 1113
Fragments arranged by size:
1561 1113 901 832 560 367 288 281
178 158 135 31
PflMI CCAn_nnn'nTGG
Cuts at: 0 477 1495 3222 3235 3828 4866 5001 6078
Size: 477 1018 1727 13 593 1038 135 1077
Cuts at: 6078 6405
Size: 327
Fragments arranged by size:
1727 1077 1038 1018 593 477 327 135
13
PfoI T'CCnGG_A
Cuts at: 0 758 2828 3920 5573 6223 6405
Size: 758 2070 1092 1653 650 182
Fragments arranged by size:
2070 1653 1092 758 650 182
PmlI CAC'GTG
Cuts at: 0 670 4007 4422 6405
Size: 670 3337 415 1983
Fragments arranged by size:
3337 1983 670 415
PpuMI rG'GwC_Cy
Cuts at: 0 48 120 705 1167 1382 1485 2355 4041
Size: 48 72 585 462 215 103 870 1686
Cuts at: 4041 4158 4761 5310 5520 6405
Size: 117 603 549 210 885
Fragments arranged by size:
1686 885 870 603 585 549 462 215
210 117 103 72 48
PshAI GACnn'nnGTC
Cuts at: 0 3865 6405
Size: 3865 2540
PsiI TTA'TAA
Cuts at: 0 5366 6405
Size: 5366 1039
PspOMI G'GGCC_C
Cuts at: 0 12 317 560 1299 1554 2005 2637 6405
Size: 12 305 243 739 255 451 632 3768
Fragments arranged by size:
3768 739 632 451 305 255 243 12
PspXI vC'TCGA_Gb
Cuts at: 0 379 1542 2875 6405
Size: 379 1163 1333 3530
Fragments arranged by size:
3530 1333 1163 379
PstI C_TGCA'G
Cuts at: 0 107 188 713 837 1356 2409 2975 3088
Size: 107 81 525 124 519 1053 566 113
Cuts at: 3088 3884 3955 4142 5510 5789 6405
Size: 796 71 187 1368 279 616
Fragments arranged by size:
1368 1053 796 616 566 525 519 279
187 124 113 107 81 71
PvuII CAG'CTG
Cuts at: 0 171 189 297 387 838 1098 1219 1281
Size: 171 18 108 90 451 260 121 62
Cuts at: 1281 4584 6348 6376 6405
Size: 3303 1764 28 29
Fragments arranged by size:
3303 1764 451 260 171 121 108 90
62 29 28 18
SacI G_AGCT'C
Cuts at: 0 1835 2984 6405
Size: 1835 1149 3421
Fragments arranged by size:
3421 1835 1149
SacII CC_GC'GG
Cuts at: 0 3513 6405
Size: 3513 2892
SanDI GG'GwC_CC
Cuts at: 0 120 1167 1485 6405
Size: 120 1047 318 4920
Fragments arranged by size:
4920 1047 318 120
SapI GCTCTTCn'nnn_
Cuts at: 0 26 2432 4534 4846 6405
Size: 26 2406 2102 312 1559
Fragments arranged by size:
2406 2102 1559 312 26
SbfI CC_TGCA'GG
Cuts at: 0 713 6405
Size: 713 5692
ScaI AGT'ACT
Cuts at: 0 5857 6292 6405
Size: 5857 435 113
Fragments arranged by size:
5857 435 113
SexAI A'CCwGG_T
Cuts at: 0 4076 4985 5654 6405
Size: 4076 909 669 751
Fragments arranged by size:
4076 909 751 669
SfcI C'TryA_G
Cuts at: 0 103 184 709 833 1352 2405 2971 3084
Size: 103 81 525 124 519 1053 566 113
Cuts at: 3084 3242 3880 3951 4138 5506 5785 6405
Size: 158 638 71 187 1368 279 620
Fragments arranged by size:
1368 1053 638 620 566 525 519 279
187 158 124 113 103 81 71
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 405 6405
Size: 405 6000
SfoI GGC'GCC
Cuts at: 0 367 3417 5494 6405
Size: 367 3050 2077 911
Fragments arranged by size:
3050 2077 911 367
SmaI CCC'GGG
Cuts at: 0 559 1027 2694 2896 3160 6405
Size: 559 468 1667 202 264 3245
Fragments arranged by size:
3245 1667 559 468 264 202
SmlI C'TyrA_G
Cuts at: 0 379 746 1542 2632 2875 3870 4945 6405
Size: 379 367 796 1090 243 995 1075 1460
Fragments arranged by size:
1460 1090 1075 995 796 379 367 243
SnaBI TAC'GTA
Cuts at: 0 1815 6405
Size: 1815 4590
StuI AGG'CCT
Cuts at: 0 1927 1990 3166 4031 5691 6405
Size: 1927 63 1176 865 1660 714
Fragments arranged by size:
1927 1660 1176 865 714 63
StyI C'CwwG_G
Cuts at: 0 1154 1232 1512 2009 2212 2492 2720 3746
Size: 1154 78 280 497 203 280 228 1026
Cuts at: 3746 3939 4235 5732 6405
Size: 193 296 1497 673
Fragments arranged by size:
1497 1154 1026 673 497 296 280 280
228 203 193 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 561 1066 1252 1334 1487 1996 2097 3208
Size: 561 505 186 82 153 509 101 1111
Cuts at: 3208 4495 4669 4756 5398 6405
Size: 1287 174 87 642 1007
Fragments arranged by size:
1287 1111 1007 642 561 509 505 186
174 153 101 87 82
TatI w'GTAC_w
Cuts at: 0 638 4304 4529 5120 5855 6278 6290 6405
Size: 638 3666 225 591 735 423 12 115
Fragments arranged by size:
3666 735 638 591 423 225 115 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 760 792 1309 1322 1341 1354 2097 2129
Size: 760 32 517 13 19 13 743 32
Cuts at: 2129 2556 2588 4007 4039 5522 5554 6405
Size: 427 32 1419 32 1483 32 851
Fragments arranged by size:
1483 1419 851 760 743 517 427 32
32 32 32 32 19 13 13
XbaI T'CTAG_A
Cuts at: 0 721 6405
Size: 721 5684
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 175 525 618 1188 1193 1257 1496 2299
Size: 175 350 93 570 5 64 239 803
Cuts at: 2299 2442 3684 4242 4671 6405
Size: 143 1242 558 429 1734
Fragments arranged by size:
1734 1242 803 570 558 429 350 239
175 143 93 64 5
XhoI C'TCGA_G
Cuts at: 0 379 746 1542 2875 3870 6405
Size: 379 367 796 1333 995 2535
Fragments arranged by size:
2535 1333 995 796 379 367
XmaI C'CCGG_G
Cuts at: 0 557 1025 2692 2894 3158 6405
Size: 557 468 1667 202 264 3247
Fragments arranged by size:
3247 1667 557 468 264 202
XmnI GAAnn'nnTTC
Cuts at: 0 4627 5857 6405
Size: 4627 1230 548
Fragments arranged by size:
4627 1230 548
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuII SacI SacII SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SmaI
SmlI SnaBI StuI StyI TaqII TatI TstI XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AscI
AseI AsiSI AvrII BaeI BclI BfrBI BmtI BsiWI
BstBI ClaI CspCI DraI EcoRV FspAI HpaI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PpiI PsrI PvuI RsrII SalI SgfI
SgrAI SpeI SphI SrfI SspI SwaI Tth111I ZraI