Restriction map for
pF1KA0411
(Linear) (Six-base) MAPSORT of: KIAA0411.seq Check: 8783 from: 1 to: 3225
KIAA0411.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AccI GT'mk_AC
Cuts at: 0 2743 3225
Size: 2743 482
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 562 1533 2941 3225
Size: 481 81 971 1408 284
Fragments arranged by size:
1408 971 481 284 81
AfeI AGC'GCT
Cuts at: 0 217 1038 2685 3225
Size: 217 821 1647 540
Fragments arranged by size:
1647 821 540 217
AflIII A'CryG_T
Cuts at: 0 503 959 3225
Size: 503 456 2266
Fragments arranged by size:
2266 503 456
AgeI A'CCGG_T
Cuts at: 0 3125 3225
Size: 3125 100
AhdI GACnn_n'nnGTC
Cuts at: 0 1944 2433 2784 3225
Size: 1944 489 351 441
Fragments arranged by size:
1944 489 441 351
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 619 2857 3225
Size: 619 2238 368
Fragments arranged by size:
2238 619 368
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 238 270 556 588 3225
Size: 238 32 286 32 2637
Fragments arranged by size:
2637 286 238 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1398 1657 3225
Size: 1398 259 1568
Fragments arranged by size:
1568 1398 259
ApaI G_GGCC'C
Cuts at: 0 2569 3225
Size: 2569 656
ApaLI G'TGCA_C
Cuts at: 0 1765 3225
Size: 1765 1460
ApoI r'AATT_y
Cuts at: 0 1324 1590 3225
Size: 1324 266 1635
Fragments arranged by size:
1635 1324 266
AvaI C'yCGr_G
Cuts at: 0 181 242 2018 2555 2588 2633 2860 3147
Size: 181 61 1776 537 33 45 227 287
Cuts at: 3147 3225
Size: 78
Fragments arranged by size:
1776 537 287 227 181 78 61 45
33
AvrII C'CTAG_G
Cuts at: 0 2772 3225
Size: 2772 453
BamHI G'GATC_C
Cuts at: 0 1878 3225
Size: 1878 1347
BanI G'GyrC_C
Cuts at: 0 1426 2886 3073 3217 3225
Size: 1426 1460 187 144 8
Fragments arranged by size:
1460 1426 187 144 8
BanII G_rGCy'C
Cuts at: 0 182 458 479 2569 2653 2918 2954 3225
Size: 182 276 21 2090 84 265 36 271
Fragments arranged by size:
2090 276 271 265 182 84 36 21
BbeI G_GCGC'C
Cuts at: 0 2890 3077 3225
Size: 2890 187 148
Fragments arranged by size:
2890 187 148
BbsI GAAGACnn'nnnn_
Cuts at: 0 966 1616 2819 2830 3225
Size: 966 650 1203 11 395
Fragments arranged by size:
1203 966 650 395 11
BbvCI CC'TCA_GC
Cuts at: 0 592 3225
Size: 592 2633
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2158 2192 2974 3008 3225
Size: 2158 34 782 34 217
Fragments arranged by size:
2158 782 217 34 34
BfrBI ATG'CAT
Cuts at: 0 2051 3225
Size: 2051 1174
BglI GCCn_nnn'nGGC
Cuts at: 0 829 3067 3071 3108 3225
Size: 829 2238 4 37 117
Fragments arranged by size:
2238 829 117 37 4
BlpI GC'TnA_GC
Cuts at: 0 212 1040 3201 3225
Size: 212 828 2161 24
Fragments arranged by size:
2161 828 212 24
Bme1580I G_kGCm'C
Cuts at: 0 1769 2569 2732 3220 3225
Size: 1769 800 163 488 5
Fragments arranged by size:
1769 800 488 163 5
BmrI ACTGGGnnnn_n'
Cuts at: 0 1848 3225
Size: 1848 1377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 237 2469 2855 3225
Size: 237 2232 386 370
Fragments arranged by size:
2232 386 370 237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 130 220 892 1516 1765 2078 2416
Size: 124 6 90 672 624 249 313 338
Cuts at: 2416 2812 2932 3225
Size: 396 120 293
Fragments arranged by size:
672 624 396 338 313 293 249 124
120 90 6
Bpu10I CC'TnA_GC
Cuts at: 0 592 2359 3225
Size: 592 1767 866
Fragments arranged by size:
1767 866 592
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 973 1090 1372 3225
Size: 973 117 282 1853
Fragments arranged by size:
1853 973 282 117
BsaI GGTCTCn'nnnn_
Cuts at: 0 326 581 868 1243 3225
Size: 326 255 287 375 1982
Fragments arranged by size:
1982 375 326 287 255
BsaHI Gr'CG_yC
Cuts at: 0 2523 2887 3074 3172 3225
Size: 2523 364 187 98 53
Fragments arranged by size:
2523 364 187 98 53
BsaWI w'CCGG_w
Cuts at: 0 842 3125 3225
Size: 842 2283 100
Fragments arranged by size:
2283 842 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 238 268 556 586 1834 1864 2254 2284
Size: 238 30 288 30 1248 30 390 30
Cuts at: 2284 3225
Size: 941
Fragments arranged by size:
1248 941 390 288 238 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 213 265 466 577 1135 1296 3009 3225
Size: 213 52 201 111 558 161 1713 216
Fragments arranged by size:
1713 558 216 213 201 161 111 52
BseYI C'CCAG_C
Cuts at: 0 626 1752 2011 2569 2605 3077 3225
Size: 626 1126 259 558 36 472 148
Fragments arranged by size:
1126 626 558 472 259 148 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2827 3225
Size: 2827 398
BsiEI CG_ry'CG
Cuts at: 0 1219 3112 3167 3225
Size: 1219 1893 55 58
Fragments arranged by size:
1893 1219 58 55
BsiHKAI G_wGCw'C
Cuts at: 0 182 251 458 479 1769 2450 2836 3225
Size: 182 69 207 21 1290 681 386 389
Fragments arranged by size:
1290 681 389 386 207 182 69 21
BsmI GAATG_Cn'
Cuts at: 0 915 1426 3225
Size: 915 511 1799
Fragments arranged by size:
1799 915 511
Bsp1286I G_dGCh'C
Cuts at: 0 182 251 458 479 1769 2450 2569 2653
Size: 182 69 207 21 1290 681 119 84
Cuts at: 2653 2732 2836 2918 2954 3220 3225
Size: 79 104 82 36 266 5
Fragments arranged by size:
1290 681 266 207 182 119 104 84
82 79 69 36 21 5
BspEI T'CCGG_A
Cuts at: 0 842 3225
Size: 842 2383
BspHI T'CATG_A
Cuts at: 0 359 488 1817 1995 3225
Size: 359 129 1329 178 1230
Fragments arranged by size:
1329 1230 359 178 129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 611 2755 3225
Size: 611 2144 470
Fragments arranged by size:
2144 611 470
BsrDI GCAATG_nn'
Cuts at: 0 38 1271 2395 3225
Size: 38 1233 1124 830
Fragments arranged by size:
1233 1124 830 38
BsrFI r'CCGG_y
Cuts at: 0 620 2279 3111 3125 3225
Size: 620 1659 832 14 100
Fragments arranged by size:
1659 832 620 100 14
BsrGI T'GTAC_A
Cuts at: 0 2325 3225
Size: 2325 900
BssSI C'ACGA_G
Cuts at: 0 448 613 2845 3225
Size: 448 165 2232 380
Fragments arranged by size:
2232 448 380 165
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2850 3225
Size: 2850 375
BstEII G'GTnAC_C
Cuts at: 0 465 3225
Size: 465 2760
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2125 3225
Size: 2125 1100
BstYI r'GATC_y
Cuts at: 0 68 1544 1561 1772 1878 3225
Size: 68 1476 17 211 106 1347
Fragments arranged by size:
1476 1347 211 106 68 17
Bsu36I CC'TnA_GG
Cuts at: 0 1706 3225
Size: 1706 1519
BtgI C'CryG_G
Cuts at: 0 2441 3225
Size: 2441 784
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2162 3225
Size: 2162 1063
BtsI GCAGTG_nn'
Cuts at: 0 920 2394 3225
Size: 920 1474 831
Fragments arranged by size:
1474 920 831
ClaI AT'CG_AT
Cuts at: 0 797 2105 3225
Size: 797 1308 1120
Fragments arranged by size:
1308 1120 797
DraI TTT'AAA
Cuts at: 0 1329 1667 3225
Size: 1329 338 1558
Fragments arranged by size:
1558 1329 338
DrdI GACnn_nn'nnGTC
Cuts at: 0 1189 3225
Size: 1189 2036
EaeI y'GGCC_r
Cuts at: 0 1097 1892 3109 3164 3225
Size: 1097 795 1217 55 61
Fragments arranged by size:
1217 1097 795 61 55
EagI C'GGCC_G
Cuts at: 0 3109 3164 3225
Size: 3109 55 61
Fragments arranged by size:
3109 61 55
EarI CTCTTCn'nnn_
Cuts at: 0 418 664 1834 2421 3225
Size: 418 246 1170 587 804
Fragments arranged by size:
1170 804 587 418 246
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 148 367 3048 3073 3225
Size: 148 219 2681 25 152
Fragments arranged by size:
2681 219 152 148 25
EcoICRI GAG'CTC
Cuts at: 0 180 456 477 3225
Size: 180 276 21 2748
Fragments arranged by size:
2748 276 180 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 130 220 481 562 892 1516 1533
Size: 124 6 90 261 81 330 624 17
Cuts at: 1533 1765 2078 2416 2812 2932 2941 3225
Size: 232 313 338 396 120 9 284
Fragments arranged by size:
624 396 338 330 313 284 261 232
124 120 90 81 17 9 6
EcoO109I rG'GnC_Cy
Cuts at: 0 1938 2032 2231 2559 2566 2802 2935 3225
Size: 1938 94 199 328 7 236 133 290
Fragments arranged by size:
1938 328 290 236 199 133 94 7
EcoRV GAT'ATC
Cuts at: 0 1644 1730 3225
Size: 1644 86 1495
Fragments arranged by size:
1644 1495 86
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1633 3225
Size: 1633 1592
HaeII r_GCGC'y
Cuts at: 0 219 633 1040 2687 2719 2890 3077 3225
Size: 219 414 407 1647 32 171 187 148
Fragments arranged by size:
1647 414 407 219 187 171 148 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 1714 1746 1930 1962 2332 2364
Size: 97 32 1585 32 184 32 370 32
Cuts at: 2364 2398 2419 2430 2451 2498 2530 2770 2802
Size: 34 21 11 21 47 32 240 32
Cuts at: 2802 3225
Size: 423
Fragments arranged by size:
1585 423 370 240 184 97 47 34
32 32 32 32 32 32 21 21
11
HincII GTy'rAC
Cuts at: 0 2115 2744 3225
Size: 2115 629 481
Fragments arranged by size:
2115 629 481
KasI G'GCGC_C
Cuts at: 0 2886 3073 3225
Size: 2886 187 152
Fragments arranged by size:
2886 187 152
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1230 1800 2375 3225
Size: 1230 570 575 850
Fragments arranged by size:
1230 850 575 570
MscI TGG'CCA
Cuts at: 0 1099 1894 3225
Size: 1099 795 1331
Fragments arranged by size:
1331 1099 795
MslI CAynn'nnrTG
Cuts at: 0 358 487 952 958 1964 2123 3028 3225
Size: 358 129 465 6 1006 159 905 197
Fragments arranged by size:
1006 905 465 358 197 159 129 6
MspA1I CmG'CkG
Cuts at: 0 81 132 859 1083 3205 3225
Size: 81 51 727 224 2122 20
Fragments arranged by size:
2122 727 224 81 51 20
NaeI GCC'GGC
Cuts at: 0 2281 3225
Size: 2281 944
NarI GG'CG_CC
Cuts at: 0 2887 3074 3225
Size: 2887 187 151
Fragments arranged by size:
2887 187 151
NgoMIV G'CCGG_C
Cuts at: 0 2279 3225
Size: 2279 946
NruI TCG'CGA
Cuts at: 0 123 3225
Size: 123 3102
NsiI A_TGCA'T
Cuts at: 0 2053 3225
Size: 2053 1172
NspI r_CATG'y
Cuts at: 0 507 963 1170 3225
Size: 507 456 207 2055
Fragments arranged by size:
2055 507 456 207
PasI CC'CwG_GG
Cuts at: 0 1398 3225
Size: 1398 1827
PciI A'CATG_T
Cuts at: 0 503 959 3225
Size: 503 456 2266
Fragments arranged by size:
2266 503 456
PfoI T'CCnGG_A
Cuts at: 0 146 3225
Size: 146 3079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 556 588 3172 3204 3225
Size: 238 32 286 32 2584 32 21
Fragments arranged by size:
2584 286 238 32 32 32 21
PpuMI rG'GwC_Cy
Cuts at: 0 1938 2032 3225
Size: 1938 94 1193
Fragments arranged by size:
1938 1193 94
PspOMI G'GGCC_C
Cuts at: 0 2565 3225
Size: 2565 660
PspXI vC'TCGA_Gb
Cuts at: 0 181 3225
Size: 181 3044
PstI C_TGCA'G
Cuts at: 0 131 443 1088 1370 3225
Size: 131 312 645 282 1855
Fragments arranged by size:
1855 645 312 282 131
PvuI CG_AT'CG
Cuts at: 0 1219 3225
Size: 1219 2006
PvuII CAG'CTG
Cuts at: 0 81 132 859 1083 3225
Size: 81 51 727 224 2142
Fragments arranged by size:
2142 727 224 81 51
SacI G_AGCT'C
Cuts at: 0 182 458 479 3225
Size: 182 276 21 2746
Fragments arranged by size:
2746 276 182 21
SalI G'TCGA_C
Cuts at: 0 2742 3225
Size: 2742 483
ScaI AGT'ACT
Cuts at: 0 187 685 3225
Size: 187 498 2540
Fragments arranged by size:
2540 498 187
SfcI C'TryA_G
Cuts at: 0 127 439 1084 1366 3225
Size: 127 312 645 282 1859
Fragments arranged by size:
1859 645 312 282 127
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3108 3225
Size: 3108 117
SfoI GGC'GCC
Cuts at: 0 2888 3075 3225
Size: 2888 187 150
Fragments arranged by size:
2888 187 150
SmaI CCC'GGG
Cuts at: 0 244 2020 2557 2590 2635 3225
Size: 244 1776 537 33 45 590
Fragments arranged by size:
1776 590 537 244 45 33
SmlI C'TyrA_G
Cuts at: 0 181 988 1105 1387 2860 3225
Size: 181 807 117 282 1473 365
Fragments arranged by size:
1473 807 365 282 181 117
SspI AAT'ATT
Cuts at: 0 2071 3225
Size: 2071 1154
StuI AGG'CCT
Cuts at: 0 2413 3225
Size: 2413 812
StyI C'CwwG_G
Cuts at: 0 2772 3225
Size: 2772 453
TatI w'GTAC_w
Cuts at: 0 185 683 2315 2325 3225
Size: 185 498 1632 10 900
Fragments arranged by size:
1632 900 498 185 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1553 1585 2254 2286 3225
Size: 1553 32 669 32 939
Fragments arranged by size:
1553 939 669 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1224 3225
Size: 1224 2001
XhoI C'TCGA_G
Cuts at: 0 181 2860 3225
Size: 181 2679 365
Fragments arranged by size:
2679 365 181
XmaI C'CCGG_G
Cuts at: 0 242 2018 2555 2588 2633 3225
Size: 242 1776 537 33 45 592
Fragments arranged by size:
1776 592 537 242 45 33
XmnI GAAnn'nnTTC
Cuts at: 0 571 1715 3187 3225
Size: 571 1144 1472 38
Fragments arranged by size:
1472 1144 571 38
Enzymes that do cut:
AccI AcuI AfeI AflIII AgeI AhdI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BfrBI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NruI
NsiI NspI PasI PciI PfoI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII SacI SalI ScaI SfcI
SfiI SfoI SmaI SmlI SspI StuI StyI TatI
TstI Tth111I XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AleI AscI AseI
AsiSI BaeI BciVI BclI BglII BmgBI BmtI BsaAI
BsaBI BsiWI BsmBI BsrBI BssHII BstBI BstZ17I CspCI
DraIII EcoNI EcoRI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI NcoI NdeI NheI NotI PacI
PflMI PmeI PmlI PshAI PsiI PsrI RsrII SacII
SanDI SapI SbfI SexAI SgfI SgrAI SnaBI SpeI
SphI SrfI SwaI TaqII XbaI XcmI ZraI