Restriction map for
pF1KSDA0414
(Linear) (Six-base) MAPSORT of: KIAA0414.seq Check: 9564 from: 1 to: 1401
KIAA0414.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 930 1401
Size: 930 471
AatII G_ACGT'C
Cuts at: 0 104 276 1401
Size: 104 172 1125
Fragments arranged by size:
1125 172 104
AccI GT'mk_AC
Cuts at: 0 347 1401
Size: 347 1054
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1401
Size: 380 1021
AfeI AGC'GCT
Cuts at: 0 748 1401
Size: 748 653
AhdI GACnn_n'nnGTC
Cuts at: 0 420 552 1401
Size: 420 132 849
Fragments arranged by size:
849 420 132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 793 1401
Size: 793 608
AlwNI CAG_nnn'CTG
Cuts at: 0 66 1105 1401
Size: 66 1039 296
Fragments arranged by size:
1039 296 66
ApaLI G'TGCA_C
Cuts at: 0 778 1401
Size: 778 623
ApoI r'AATT_y
Cuts at: 0 31 1222 1259 1401
Size: 31 1191 37 142
Fragments arranged by size:
1191 142 37 31
AvaI C'yCGr_G
Cuts at: 0 742 1401
Size: 742 659
BamHI G'GATC_C
Cuts at: 0 728 1401
Size: 728 673
BanI G'GyrC_C
Cuts at: 0 652 1401
Size: 652 749
BanII G_rGCy'C
Cuts at: 0 475 1401
Size: 475 926
BbeI G_GCGC'C
Cuts at: 0 656 1401
Size: 656 745
BbsI GAAGACnn'nnnn_
Cuts at: 0 1180 1401
Size: 1180 221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1164 1198 1401
Size: 1164 34 203
Fragments arranged by size:
1164 203 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1127 1401
Size: 1127 274
BlpI GC'TnA_GC
Cuts at: 0 1376 1401
Size: 1376 25
Bme1580I G_kGCm'C
Cuts at: 0 138 782 1010 1401
Size: 138 644 228 391
Fragments arranged by size:
644 391 228 138
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 489 1401
Size: 489 912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 313 1401
Size: 275 38 1088
Fragments arranged by size:
1088 275 38
Bpu10I CC'TnA_GC
Cuts at: 0 616 1401
Size: 616 785
BsaAI yAC'GTr
Cuts at: 0 735 1401
Size: 735 666
BsaBI GATnn'nnATC
Cuts at: 0 995 1401
Size: 995 406
BsaHI Gr'CG_yC
Cuts at: 0 101 273 653 1401
Size: 101 172 380 748
Fragments arranged by size:
748 380 172 101
BsaWI w'CCGG_w
Cuts at: 0 976 1401
Size: 976 425
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1308 1338 1401
Size: 1308 30 63
Fragments arranged by size:
1308 63 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 547 661 877 1401
Size: 547 114 216 524
Fragments arranged by size:
547 524 216 114
BseYI C'CCAG_C
Cuts at: 0 468 560 586 703 1401
Size: 468 92 26 117 698
Fragments arranged by size:
698 468 117 92 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 865 1401
Size: 847 18 536
Fragments arranged by size:
847 536 18
BsiHKAI G_wGCw'C
Cuts at: 0 541 575 622 782 800 839 1401
Size: 541 34 47 160 18 39 562
Fragments arranged by size:
562 541 160 47 39 34 18
BsmBI CGTCTCn'nnnn_
Cuts at: 0 108 1401
Size: 108 1293
Bsp1286I G_dGCh'C
Cuts at: 0 138 475 541 575 622 782 800 839
Size: 138 337 66 34 47 160 18 39
Cuts at: 839 1010 1401
Size: 171 391
Fragments arranged by size:
391 337 171 160 138 66 47 39
34 18
BspEI T'CCGG_A
Cuts at: 0 976 1401
Size: 976 425
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 590 930 1024 1401
Size: 590 340 94 377
Fragments arranged by size:
590 377 340 94
BsrBI CCG'CTC
Cuts at: 0 289 1401
Size: 289 1112
BsrFI r'CCGG_y
Cuts at: 0 1331 1401
Size: 1331 70
BsrGI T'GTAC_A
Cuts at: 0 692 1401
Size: 692 709
BssSI C'ACGA_G
Cuts at: 0 1243 1401
Size: 1243 158
BstAPI GCAn_nnn'nTGC
Cuts at: 0 843 1401
Size: 843 558
BstXI CCAn_nnnn'nTGG
Cuts at: 0 711 1401
Size: 711 690
BstYI r'GATC_y
Cuts at: 0 728 1401
Size: 728 673
BtgI C'CryG_G
Cuts at: 0 962 1401
Size: 962 439
BtsI GCAGTG_nn'
Cuts at: 0 417 1401
Size: 417 984
DraIII CAC_nnn'GTG
Cuts at: 0 843 1401
Size: 843 558
EarI CTCTTCn'nnn_
Cuts at: 0 524 962 963 1401
Size: 524 438 1 438
Fragments arranged by size:
524 438 438 1
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 313 380 1401
Size: 275 38 67 1021
Fragments arranged by size:
1021 275 67 38
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 166 1401
Size: 166 1235
HaeII r_GCGC'y
Cuts at: 0 81 656 668 750 1401
Size: 81 575 12 82 651
Fragments arranged by size:
651 575 82 81 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 406 438 538 541 570 573 1401
Size: 406 32 100 3 29 3 828
Fragments arranged by size:
828 406 100 32 29 3 3
HindIII A'AGCT_T
Cuts at: 0 321 1079 1401
Size: 321 758 322
Fragments arranged by size:
758 322 321
KasI G'GCGC_C
Cuts at: 0 652 1401
Size: 652 749
MslI CAynn'nnrTG
Cuts at: 0 410 625 775 1174 1334 1401
Size: 410 215 150 399 160 67
Fragments arranged by size:
410 399 215 160 150 67
MspA1I CmG'CkG
Cuts at: 0 316 1401
Size: 316 1085
NarI GG'CG_CC
Cuts at: 0 653 1401
Size: 653 748
NcoI C'CATG_G
Cuts at: 0 962 1401
Size: 962 439
NspI r_CATG'y
Cuts at: 0 343 1179 1339 1401
Size: 343 836 160 62
Fragments arranged by size:
836 343 160 62
PflMI CCAn_nnn'nTGG
Cuts at: 0 1245 1401
Size: 1245 156
PmlI CAC'GTG
Cuts at: 0 735 1401
Size: 735 666
PsiI TTA'TAA
Cuts at: 0 443 1401
Size: 443 958
SapI GCTCTTCn'nnn_
Cuts at: 0 963 1401
Size: 963 438
SexAI A'CCwGG_T
Cuts at: 0 179 800 1401
Size: 179 621 601
Fragments arranged by size:
621 601 179
SfcI C'TryA_G
Cuts at: 0 58 380 1401
Size: 58 322 1021
Fragments arranged by size:
1021 322 58
SfoI GGC'GCC
Cuts at: 0 654 1401
Size: 654 747
SphI G_CATG'C
Cuts at: 0 1179 1401
Size: 1179 222
SspI AAT'ATT
Cuts at: 0 1050 1401
Size: 1050 351
StyI C'CwwG_G
Cuts at: 0 117 962 1401
Size: 117 845 439
Fragments arranged by size:
845 439 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1174 1401
Size: 1174 227
TatI w'GTAC_w
Cuts at: 0 692 1348 1401
Size: 692 656 53
Fragments arranged by size:
692 656 53
XmnI GAAnn'nnTTC
Cuts at: 0 1138 1401
Size: 1138 263
ZraI GAC'GTC
Cuts at: 0 102 274 1401
Size: 102 172 1127
Fragments arranged by size:
1127 172 102
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AhdI AlfI AlwNI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BlpI Bme1580I BplI BpmI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssSI
BstAPI BstXI BstYI BtgI BtsI DraIII EarI Eco57MI
FalI HaeII Hin4I HindIII KasI MslI MspA1I NarI
NcoI NspI PflMI PmlI PsiI SapI SexAI SfcI
SfoI SphI SspI StyI TaqII TatI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AleI AloI ApaI
AscI AseI AsiSI AvrII BaeI BbvCI BclI BfrBI
BglI BglII BmgBI BmrI BmtI BpuEI BsaI BsiEI
BsiWI BsmI BspHI BsrDI BssHII BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EaeI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HincII HpaI KpnI MfeI MluI MmeI MscI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI