(Linear) (Six-base) MAPSORT of: KIAA0414.seq Check: 9564 from: 1 to: 1401 KIAA0414.orf With 182 enzymes: SgfI * October 24, 2006 15:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 930 1401 Size: 930 471 AatII G_ACGT'C Cuts at: 0 104 276 1401 Size: 104 172 1125 Fragments arranged by size: 1125 172 104 AccI GT'mk_AC Cuts at: 0 347 1401 Size: 347 1054 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 380 1401 Size: 380 1021 AfeI AGC'GCT Cuts at: 0 748 1401 Size: 748 653 AhdI GACnn_n'nnGTC Cuts at: 0 420 552 1401 Size: 420 132 849 Fragments arranged by size: 849 420 132 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 793 1401 Size: 793 608 AlwNI CAG_nnn'CTG Cuts at: 0 66 1105 1401 Size: 66 1039 296 Fragments arranged by size: 1039 296 66 ApaLI G'TGCA_C Cuts at: 0 778 1401 Size: 778 623 ApoI r'AATT_y Cuts at: 0 31 1222 1259 1401 Size: 31 1191 37 142 Fragments arranged by size: 1191 142 37 31 AvaI C'yCGr_G Cuts at: 0 742 1401 Size: 742 659 BamHI G'GATC_C Cuts at: 0 728 1401 Size: 728 673 BanI G'GyrC_C Cuts at: 0 652 1401 Size: 652 749 BanII G_rGCy'C Cuts at: 0 475 1401 Size: 475 926 BbeI G_GCGC'C Cuts at: 0 656 1401 Size: 656 745 BbsI GAAGACnn'nnnn_ Cuts at: 0 1180 1401 Size: 1180 221 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1164 1198 1401 Size: 1164 34 203 Fragments arranged by size: 1164 203 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1127 1401 Size: 1127 274 BlpI GC'TnA_GC Cuts at: 0 1376 1401 Size: 1376 25 Bme1580I G_kGCm'C Cuts at: 0 138 782 1010 1401 Size: 138 644 228 391 Fragments arranged by size: 644 391 228 138 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 489 1401 Size: 489 912 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 275 313 1401 Size: 275 38 1088 Fragments arranged by size: 1088 275 38 Bpu10I CC'TnA_GC Cuts at: 0 616 1401 Size: 616 785 BsaAI yAC'GTr Cuts at: 0 735 1401 Size: 735 666 BsaBI GATnn'nnATC Cuts at: 0 995 1401 Size: 995 406 BsaHI Gr'CG_yC Cuts at: 0 101 273 653 1401 Size: 101 172 380 748 Fragments arranged by size: 748 380 172 101 BsaWI w'CCGG_w Cuts at: 0 976 1401 Size: 976 425 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1308 1338 1401 Size: 1308 30 63 Fragments arranged by size: 1308 63 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 547 661 877 1401 Size: 547 114 216 524 Fragments arranged by size: 547 524 216 114 BseYI C'CCAG_C Cuts at: 0 468 560 586 703 1401 Size: 468 92 26 117 698 Fragments arranged by size: 698 468 117 92 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 847 865 1401 Size: 847 18 536 Fragments arranged by size: 847 536 18 BsiHKAI G_wGCw'C Cuts at: 0 541 575 622 782 800 839 1401 Size: 541 34 47 160 18 39 562 Fragments arranged by size: 562 541 160 47 39 34 18 BsmBI CGTCTCn'nnnn_ Cuts at: 0 108 1401 Size: 108 1293 Bsp1286I G_dGCh'C Cuts at: 0 138 475 541 575 622 782 800 839 Size: 138 337 66 34 47 160 18 39 Cuts at: 839 1010 1401 Size: 171 391 Fragments arranged by size: 391 337 171 160 138 66 47 39 34 18 BspEI T'CCGG_A Cuts at: 0 976 1401 Size: 976 425 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 590 930 1024 1401 Size: 590 340 94 377 Fragments arranged by size: 590 377 340 94 BsrBI CCG'CTC Cuts at: 0 289 1401 Size: 289 1112 BsrFI r'CCGG_y Cuts at: 0 1331 1401 Size: 1331 70 BsrGI T'GTAC_A Cuts at: 0 692 1401 Size: 692 709 BssSI C'ACGA_G Cuts at: 0 1243 1401 Size: 1243 158 BstAPI GCAn_nnn'nTGC Cuts at: 0 843 1401 Size: 843 558 BstXI CCAn_nnnn'nTGG Cuts at: 0 711 1401 Size: 711 690 BstYI r'GATC_y Cuts at: 0 728 1401 Size: 728 673 BtgI C'CryG_G Cuts at: 0 962 1401 Size: 962 439 BtsI GCAGTG_nn' Cuts at: 0 417 1401 Size: 417 984 DraIII CAC_nnn'GTG Cuts at: 0 843 1401 Size: 843 558 EarI CTCTTCn'nnn_ Cuts at: 0 524 962 963 1401 Size: 524 438 1 438 Fragments arranged by size: 524 438 438 1 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 275 313 380 1401 Size: 275 38 67 1021 Fragments arranged by size: 1021 275 67 38 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 166 1401 Size: 166 1235 HaeII r_GCGC'y Cuts at: 0 81 656 668 750 1401 Size: 81 575 12 82 651 Fragments arranged by size: 651 575 82 81 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 406 438 538 541 570 573 1401 Size: 406 32 100 3 29 3 828 Fragments arranged by size: 828 406 100 32 29 3 3 HindIII A'AGCT_T Cuts at: 0 321 1079 1401 Size: 321 758 322 Fragments arranged by size: 758 322 321 KasI G'GCGC_C Cuts at: 0 652 1401 Size: 652 749 MslI CAynn'nnrTG Cuts at: 0 410 625 775 1174 1334 1401 Size: 410 215 150 399 160 67 Fragments arranged by size: 410 399 215 160 150 67 MspA1I CmG'CkG Cuts at: 0 316 1401 Size: 316 1085 NarI GG'CG_CC Cuts at: 0 653 1401 Size: 653 748 NcoI C'CATG_G Cuts at: 0 962 1401 Size: 962 439 NspI r_CATG'y Cuts at: 0 343 1179 1339 1401 Size: 343 836 160 62 Fragments arranged by size: 836 343 160 62 PflMI CCAn_nnn'nTGG Cuts at: 0 1245 1401 Size: 1245 156 PmlI CAC'GTG Cuts at: 0 735 1401 Size: 735 666 PsiI TTA'TAA Cuts at: 0 443 1401 Size: 443 958 SapI GCTCTTCn'nnn_ Cuts at: 0 963 1401 Size: 963 438 SexAI A'CCwGG_T Cuts at: 0 179 800 1401 Size: 179 621 601 Fragments arranged by size: 621 601 179 SfcI C'TryA_G Cuts at: 0 58 380 1401 Size: 58 322 1021 Fragments arranged by size: 1021 322 58 SfoI GGC'GCC Cuts at: 0 654 1401 Size: 654 747 SphI G_CATG'C Cuts at: 0 1179 1401 Size: 1179 222 SspI AAT'ATT Cuts at: 0 1050 1401 Size: 1050 351 StyI C'CwwG_G Cuts at: 0 117 962 1401 Size: 117 845 439 Fragments arranged by size: 845 439 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1174 1401 Size: 1174 227 TatI w'GTAC_w Cuts at: 0 692 1348 1401 Size: 692 656 53 Fragments arranged by size: 692 656 53 XmnI GAAnn'nnTTC Cuts at: 0 1138 1401 Size: 1138 263 ZraI GAC'GTC Cuts at: 0 102 274 1401 Size: 102 172 1127 Fragments arranged by size: 1127 172 102 Enzymes that do cut: AarI AatII AccI AcuI AfeI AhdI AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BlpI Bme1580I BplI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI BtsI DraIII EarI Eco57MI FalI HaeII Hin4I HindIII KasI MslI MspA1I NarI NcoI NspI PflMI PmlI PsiI SapI SexAI SfcI SfoI SphI SspI StyI TaqII TatI XmnI ZraI Enzymes that do not cut: Acc65I AclI AflII AflIII AgeI AleI AloI ApaI AscI AseI AsiSI AvrII BaeI BbvCI BclI BfrBI BglI BglII BmgBI BmrI BmtI BpuEI BsaI BsiEI BsiWI BsmI BspHI BsrDI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HincII HpaI KpnI MfeI MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI