Restriction map for pF1KSDA0414
 (Linear) (Six-base) MAPSORT of: KIAA0414.seq  Check: 9564  from: 1  to: 1401



KIAA0414.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     930    1401 
   Size:        930     471
 
AatII G_ACGT'C

Cuts at:      0     104     276    1401 
   Size:        104     172    1125

  Fragments arranged by size: 

                1125     172     104
 
AccI GT'mk_AC

Cuts at:      0     347    1401 
   Size:        347    1054
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     380    1401 
   Size:        380    1021
 
AfeI AGC'GCT

Cuts at:      0     748    1401 
   Size:        748     653
 
AhdI GACnn_n'nnGTC

Cuts at:      0     420     552    1401 
   Size:        420     132     849

  Fragments arranged by size: 

                 849     420     132
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     793    1401 
   Size:        793     608
 
AlwNI CAG_nnn'CTG

Cuts at:      0      66    1105    1401 
   Size:         66    1039     296

  Fragments arranged by size: 

                1039     296      66
 
ApaLI G'TGCA_C

Cuts at:      0     778    1401 
   Size:        778     623
 
ApoI r'AATT_y

Cuts at:      0      31    1222    1259    1401 
   Size:         31    1191      37     142

  Fragments arranged by size: 

                1191     142      37      31
 
AvaI C'yCGr_G

Cuts at:      0     742    1401 
   Size:        742     659
 
BamHI G'GATC_C

Cuts at:      0     728    1401 
   Size:        728     673
 
BanI G'GyrC_C

Cuts at:      0     652    1401 
   Size:        652     749
 
BanII G_rGCy'C

Cuts at:      0     475    1401 
   Size:        475     926
 
BbeI G_GCGC'C

Cuts at:      0     656    1401 
   Size:        656     745
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1180    1401 
   Size:       1180     221
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1164    1198    1401 
   Size:       1164      34     203

  Fragments arranged by size: 

                1164     203      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1127    1401 
   Size:       1127     274
 
BlpI GC'TnA_GC

Cuts at:      0    1376    1401 
   Size:       1376      25
 
Bme1580I G_kGCm'C

Cuts at:      0     138     782    1010    1401 
   Size:        138     644     228     391

  Fragments arranged by size: 

                 644     391     228     138
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     489    1401 
   Size:        489     912
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275     313    1401 
   Size:        275      38    1088

  Fragments arranged by size: 

                1088     275      38
 
Bpu10I CC'TnA_GC

Cuts at:      0     616    1401 
   Size:        616     785
 
BsaAI yAC'GTr

Cuts at:      0     735    1401 
   Size:        735     666
 
BsaBI GATnn'nnATC

Cuts at:      0     995    1401 
   Size:        995     406
 
BsaHI Gr'CG_yC

Cuts at:      0     101     273     653    1401 
   Size:        101     172     380     748

  Fragments arranged by size: 

                 748     380     172     101
 
BsaWI w'CCGG_w

Cuts at:      0     976    1401 
   Size:        976     425
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1308    1338    1401 
   Size:       1308      30      63

  Fragments arranged by size: 

                1308      63      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     547     661     877    1401 
   Size:        547     114     216     524

  Fragments arranged by size: 

                 547     524     216     114
 
BseYI C'CCAG_C

Cuts at:      0     468     560     586     703    1401 
   Size:        468      92      26     117     698

  Fragments arranged by size: 

                 698     468     117      92      26
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     847     865    1401 
   Size:        847      18     536

  Fragments arranged by size: 

                 847     536      18
 
BsiHKAI G_wGCw'C

Cuts at:      0     541     575     622     782     800     839    1401 
   Size:        541      34      47     160      18      39     562

  Fragments arranged by size: 

                 562     541     160      47      39      34      18
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     108    1401 
   Size:        108    1293
 
Bsp1286I G_dGCh'C

Cuts at:      0     138     475     541     575     622     782     800     839 
   Size:        138     337      66      34      47     160      18      39

Cuts at:    839    1010    1401 
   Size:        171     391

  Fragments arranged by size: 

                 391     337     171     160     138      66      47      39
                  34      18
 
BspEI T'CCGG_A

Cuts at:      0     976    1401 
   Size:        976     425
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     590     930    1024    1401 
   Size:        590     340      94     377

  Fragments arranged by size: 

                 590     377     340      94
 
BsrBI CCG'CTC

Cuts at:      0     289    1401 
   Size:        289    1112
 
BsrFI r'CCGG_y

Cuts at:      0    1331    1401 
   Size:       1331      70
 
BsrGI T'GTAC_A

Cuts at:      0     692    1401 
   Size:        692     709
 
BssSI C'ACGA_G

Cuts at:      0    1243    1401 
   Size:       1243     158
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     843    1401 
   Size:        843     558
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     711    1401 
   Size:        711     690
 
BstYI r'GATC_y

Cuts at:      0     728    1401 
   Size:        728     673
 
BtgI C'CryG_G

Cuts at:      0     962    1401 
   Size:        962     439
 
BtsI GCAGTG_nn'

Cuts at:      0     417    1401 
   Size:        417     984
 
DraIII CAC_nnn'GTG

Cuts at:      0     843    1401 
   Size:        843     558
 
EarI CTCTTCn'nnn_

Cuts at:      0     524     962     963    1401 
   Size:        524     438       1     438

  Fragments arranged by size: 

                 524     438     438       1
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275     313     380    1401 
   Size:        275      38      67    1021

  Fragments arranged by size: 

                1021     275      67      38
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     166    1401 
   Size:        166    1235
 
HaeII r_GCGC'y

Cuts at:      0      81     656     668     750    1401 
   Size:         81     575      12      82     651

  Fragments arranged by size: 

                 651     575      82      81      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     406     438     538     541     570     573    1401 
   Size:        406      32     100       3      29       3     828

  Fragments arranged by size: 

                 828     406     100      32      29       3       3
 
HindIII A'AGCT_T

Cuts at:      0     321    1079    1401 
   Size:        321     758     322

  Fragments arranged by size: 

                 758     322     321
 
KasI G'GCGC_C

Cuts at:      0     652    1401 
   Size:        652     749
 
MslI CAynn'nnrTG

Cuts at:      0     410     625     775    1174    1334    1401 
   Size:        410     215     150     399     160      67

  Fragments arranged by size: 

                 410     399     215     160     150      67
 
MspA1I CmG'CkG

Cuts at:      0     316    1401 
   Size:        316    1085
 
NarI GG'CG_CC

Cuts at:      0     653    1401 
   Size:        653     748
 
NcoI C'CATG_G

Cuts at:      0     962    1401 
   Size:        962     439
 
NspI r_CATG'y

Cuts at:      0     343    1179    1339    1401 
   Size:        343     836     160      62

  Fragments arranged by size: 

                 836     343     160      62
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1245    1401 
   Size:       1245     156
 
PmlI CAC'GTG

Cuts at:      0     735    1401 
   Size:        735     666
 
PsiI TTA'TAA

Cuts at:      0     443    1401 
   Size:        443     958
 
SapI GCTCTTCn'nnn_

Cuts at:      0     963    1401 
   Size:        963     438
 
SexAI A'CCwGG_T

Cuts at:      0     179     800    1401 
   Size:        179     621     601

  Fragments arranged by size: 

                 621     601     179
 
SfcI C'TryA_G

Cuts at:      0      58     380    1401 
   Size:         58     322    1021

  Fragments arranged by size: 

                1021     322      58
 
SfoI GGC'GCC

Cuts at:      0     654    1401 
   Size:        654     747
 
SphI G_CATG'C

Cuts at:      0    1179    1401 
   Size:       1179     222
 
SspI AAT'ATT

Cuts at:      0    1050    1401 
   Size:       1050     351
 
StyI C'CwwG_G

Cuts at:      0     117     962    1401 
   Size:        117     845     439

  Fragments arranged by size: 

                 845     439     117
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1174    1401 
   Size:       1174     227
 
TatI w'GTAC_w

Cuts at:      0     692    1348    1401 
   Size:        692     656      53

  Fragments arranged by size: 

                 692     656      53
 
XmnI GAAnn'nnTTC

Cuts at:      0    1138    1401 
   Size:       1138     263
 
ZraI GAC'GTC

Cuts at:      0     102     274    1401 
   Size:        102     172    1127

  Fragments arranged by size: 

                1127     172     102
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI     AhdI     AlfI    AlwNI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
     BcgI    BciVI     BlpI Bme1580I     BplI     BpmI   Bpu10I    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BsrGI    BssSI
   BstAPI    BstXI    BstYI     BtgI     BtsI   DraIII     EarI  Eco57MI
     FalI    HaeII    Hin4I  HindIII     KasI     MslI   MspA1I     NarI
     NcoI     NspI    PflMI     PmlI     PsiI     SapI    SexAI     SfcI
     SfoI     SphI     SspI     StyI    TaqII     TatI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII   AflIII     AgeI     AleI     AloI     ApaI
     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI     BclI    BfrBI
     BglI    BglII    BmgBI     BmrI     BmtI    BpuEI     BsaI    BsiEI
    BsiWI     BsmI    BspHI    BsrDI   BssHII    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI   HincII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SrfI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI
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