Restriction map for
pF1KSDA0422
(Linear) (Six-base) MAPSORT of: KIAA0422.seq Check: 9357 from: 1 to: 3345
KIAA0422.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 94 360 998 2574 3112 3345
Size: 94 266 638 1576 538 233
Fragments arranged by size:
1576 638 538 266 233 94
AccI GT'mk_AC
Cuts at: 0 737 1355 2593 3345
Size: 737 618 1238 752
Fragments arranged by size:
1238 752 737 618
AflIII A'CryG_T
Cuts at: 0 1616 2279 3345
Size: 1616 663 1066
Fragments arranged by size:
1616 1066 663
AleI CACnn'nnGTG
Cuts at: 0 490 1337 3345
Size: 490 847 2008
Fragments arranged by size:
2008 847 490
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 884 1197 2131 3345
Size: 884 313 934 1214
Fragments arranged by size:
1214 934 884 313
AlwNI CAG_nnn'CTG
Cuts at: 0 1197 1569 3345
Size: 1197 372 1776
Fragments arranged by size:
1776 1197 372
ApaI G_GGCC'C
Cuts at: 0 178 3112 3337 3345
Size: 178 2934 225 8
Fragments arranged by size:
2934 225 178 8
ApaLI G'TGCA_C
Cuts at: 0 1182 1438 3345
Size: 1182 256 1907
Fragments arranged by size:
1907 1182 256
AvaI C'yCGr_G
Cuts at: 0 98 1316 1550 1715 2726 3345
Size: 98 1218 234 165 1011 619
Fragments arranged by size:
1218 1011 619 234 165 98
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 269 302 1549 1582 3345
Size: 269 33 1247 33 1763
Fragments arranged by size:
1763 1247 269 33 33
BamHI G'GATC_C
Cuts at: 0 1995 3345
Size: 1995 1350
BanI G'GyrC_C
Cuts at: 0 86 185 318 330 356 719 868 1660
Size: 86 99 133 12 26 363 149 792
Cuts at: 1660 1837 3345
Size: 177 1508
Fragments arranged by size:
1508 792 363 177 149 133 99 86
26 12
BanII G_rGCy'C
Cuts at: 0 178 581 1220 2381 3112 3138 3337 3345
Size: 178 403 639 1161 731 26 199 8
Fragments arranged by size:
1161 731 639 403 199 178 26 8
BbeI G_GCGC'C
Cuts at: 0 90 322 360 1664 3345
Size: 90 232 38 1304 1681
Fragments arranged by size:
1681 1304 232 90 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 1073 1094 2028 2319 3345
Size: 1073 21 934 291 1026
Fragments arranged by size:
1073 1026 934 291 21
BbvCI CC'TCA_GC
Cuts at: 0 311 527 784 3345
Size: 311 216 257 2561
Fragments arranged by size:
2561 311 257 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 985 1019 1696 1730 3345
Size: 985 34 677 34 1615
Fragments arranged by size:
1615 985 677 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 270 3345
Size: 270 3075
BclI T'GATC_A
Cuts at: 0 1911 3345
Size: 1911 1434
BglI GCCn_nnn'nGGC
Cuts at: 0 541 1532 1604 1696 1805 3345
Size: 541 991 72 92 109 1540
Fragments arranged by size:
1540 991 541 109 92 72
BglII A'GATC_T
Cuts at: 0 1106 1271 3345
Size: 1106 165 2074
Fragments arranged by size:
2074 1106 165
BlpI GC'TnA_GC
Cuts at: 0 2579 3345
Size: 2579 766
Bme1580I G_kGCm'C
Cuts at: 0 178 190 335 724 1186 1442 1840 2028
Size: 178 12 145 389 462 256 398 188
Cuts at: 2028 2220 3112 3337 3345
Size: 192 892 225 8
Fragments arranged by size:
892 462 398 389 256 225 192 188
178 145 12 8
BmgBI CAC'GTC
Cuts at: 0 2706 3345
Size: 2706 639
BmrI ACTGGGnnnn_n'
Cuts at: 0 1173 3106 3345
Size: 1173 1933 239
Fragments arranged by size:
1933 1173 239
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1389 3345
Size: 1389 1956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 2845 3289 3345
Size: 427 2418 444 56
Fragments arranged by size:
2418 444 427 56
Bpu10I CC'TnA_GC
Cuts at: 0 311 527 784 1882 3345
Size: 311 216 257 1098 1463
Fragments arranged by size:
1463 1098 311 257 216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1609 1990 3345
Size: 1609 381 1355
Fragments arranged by size:
1609 1355 381
BsaI GGTCTCn'nnnn_
Cuts at: 0 952 2470 3345
Size: 952 1518 875
Fragments arranged by size:
1518 952 875
BsaAI yAC'GTr
Cuts at: 0 639 1384 3345
Size: 639 745 1961
Fragments arranged by size:
1961 745 639
BsaBI GATnn'nnATC
Cuts at: 0 1270 3345
Size: 1270 2075
BsaHI Gr'CG_yC
Cuts at: 0 87 203 319 357 1448 1661 2450 3345
Size: 87 116 116 38 1091 213 789 895
Fragments arranged by size:
1091 895 789 213 116 116 87 38
BsaWI w'CCGG_w
Cuts at: 0 212 2672 3345
Size: 212 2460 673
Fragments arranged by size:
2460 673 212
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 400 430 2000 2030 3345
Size: 400 30 1570 30 1315
Fragments arranged by size:
1570 1315 400 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 745 1696 2659 2668 2917 3345
Size: 745 951 963 9 249 428
Fragments arranged by size:
963 951 745 428 249 9
BseYI C'CCAG_C
Cuts at: 0 157 243 645 1040 1748 2403 2412 3337
Size: 157 86 402 395 708 655 9 925
Cuts at: 3337 3345
Size: 8
Fragments arranged by size:
925 708 655 402 395 157 86 9
8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 388 685 876 897 2317 3345
Size: 99 289 297 191 21 1420 1028
Fragments arranged by size:
1420 1028 297 289 191 99 21
BsiEI CG_ry'CG
Cuts at: 0 3213 3345
Size: 3213 132
BsiHKAI G_wGCw'C
Cuts at: 0 1186 1220 1442 3068 3345
Size: 1186 34 222 1626 277
Fragments arranged by size:
1626 1186 277 222 34
Bsp1286I G_dGCh'C
Cuts at: 0 178 190 335 581 724 1186 1220 1442
Size: 178 12 145 246 143 462 34 222
Cuts at: 1442 1840 2028 2220 2381 3068 3112 3138 3337
Size: 398 188 192 161 687 44 26 199
Cuts at: 3337 3345
Size: 8
Fragments arranged by size:
687 462 398 246 222 199 192 188
178 161 145 143 44 34 26 12
8
BspEI T'CCGG_A
Cuts at: 0 212 3345
Size: 212 3133
BspHI T'CATG_A
Cuts at: 0 1202 3345
Size: 1202 2143
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 94 360 657 998 2574 3112 3345
Size: 94 266 297 341 1576 538 233
Fragments arranged by size:
1576 538 341 297 266 233 94
BsrBI CCG'CTC
Cuts at: 0 1017 2210 2907 2999 3345
Size: 1017 1193 697 92 346
Fragments arranged by size:
1193 1017 697 346 92
BsrDI GCAATG_nn'
Cuts at: 0 72 2270 2745 2792 3345
Size: 72 2198 475 47 553
Fragments arranged by size:
2198 553 475 72 47
BsrFI r'CCGG_y
Cuts at: 0 596 1937 3345
Size: 596 1341 1408
Fragments arranged by size:
1408 1341 596
BstEII G'GTnAC_C
Cuts at: 0 3222 3345
Size: 3222 123
BstXI CCAn_nnnn'nTGG
Cuts at: 0 627 1377 1512 1728 2706 3345
Size: 627 750 135 216 978 639
Fragments arranged by size:
978 750 639 627 216 135
BstYI r'GATC_y
Cuts at: 0 1106 1271 1965 1995 2096 3345
Size: 1106 165 694 30 101 1249
Fragments arranged by size:
1249 1106 694 165 101 30
Bsu36I CC'TnA_GG
Cuts at: 0 1261 1859 2174 2961 3345
Size: 1261 598 315 787 384
Fragments arranged by size:
1261 787 598 384 315
BtgI C'CryG_G
Cuts at: 0 90 837 963 1052 1599 1727 2454 3227
Size: 90 747 126 89 547 128 727 773
Cuts at: 3227 3345
Size: 118
Fragments arranged by size:
773 747 727 547 128 126 118 90
89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 726 2859 3345
Size: 726 2133 486
Fragments arranged by size:
2133 726 486
BtsI GCAGTG_nn'
Cuts at: 0 263 668 1184 1254 1332 1440 1558 3008
Size: 263 405 516 70 78 108 118 1450
Cuts at: 3008 3345
Size: 337
Fragments arranged by size:
1450 516 405 337 263 118 108 78
70
ClaI AT'CG_AT
Cuts at: 0 1895 3345
Size: 1895 1450
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2406 2441 3345
Size: 2406 35 904
Fragments arranged by size:
2406 904 35
EaeI y'GGCC_r
Cuts at: 0 398 1502 1533 1697 2315 2357 3345
Size: 398 1104 31 164 618 42 988
Fragments arranged by size:
1104 988 618 398 164 42 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 598 1786 3345
Size: 598 1188 1559
Fragments arranged by size:
1559 1188 598
EcoICRI GAG'CTC
Cuts at: 0 1218 3345
Size: 1218 2127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 2845 3289 3345
Size: 427 2418 444 56
Fragments arranged by size:
2418 444 427 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3238 3345
Size: 3238 107
EcoO109I rG'GnC_Cy
Cuts at: 0 175 2178 2407 3204 3333 3334 3345
Size: 175 2003 229 797 129 1 11
Fragments arranged by size:
2003 797 229 175 129 11 1
FspI TGC'GCA
Cuts at: 0 1190 3345
Size: 1190 2155
HaeII r_GCGC'y
Cuts at: 0 90 322 360 414 680 945 1664 2566
Size: 90 232 38 54 266 265 719 902
Cuts at: 2566 2736 3345
Size: 170 609
Fragments arranged by size:
902 719 609 266 265 232 170 90
54 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1468 1500 1950 1982 3269 3301 3345
Size: 1468 32 450 32 1287 32 44
Fragments arranged by size:
1468 1287 450 44 32 32 32
HincII GTy'rAC
Cuts at: 0 2594 3345
Size: 2594 751
KasI G'GCGC_C
Cuts at: 0 86 318 356 1660 3345
Size: 86 232 38 1304 1685
Fragments arranged by size:
1685 1304 232 86 38
MluI A'CGCG_T
Cuts at: 0 1616 3345
Size: 1616 1729
MscI TGG'CCA
Cuts at: 0 1504 1699 2317 2359 3345
Size: 1504 195 618 42 986
Fragments arranged by size:
1504 986 618 195 42
MslI CAynn'nnrTG
Cuts at: 0 490 586 625 766 1057 1127 1179 1337
Size: 490 96 39 141 291 70 52 158
Cuts at: 1337 1414 2086 2950 3059 3345
Size: 77 672 864 109 286
Fragments arranged by size:
864 672 490 291 286 158 141 109
96 77 70 52 39
MspA1I CmG'CkG
Cuts at: 0 298 423 490 788 965 1430 1710 1917
Size: 298 125 67 298 177 465 280 207
Cuts at: 1917 2503 2919 3267 3345
Size: 586 416 348 78
Fragments arranged by size:
586 465 416 348 298 298 280 207
177 125 78 67
NarI GG'CG_CC
Cuts at: 0 87 319 357 1661 3345
Size: 87 232 38 1304 1684
Fragments arranged by size:
1684 1304 232 87 38
NcoI C'CATG_G
Cuts at: 0 1052 1727 2454 3345
Size: 1052 675 727 891
Fragments arranged by size:
1052 891 727 675
NspI r_CATG'y
Cuts at: 0 771 1413 2283 2301 2578 3345
Size: 771 642 870 18 277 767
Fragments arranged by size:
870 771 767 642 277 18
PasI CC'CwG_GG
Cuts at: 0 264 3345
Size: 264 3081
PciI A'CATG_T
Cuts at: 0 2279 3345
Size: 2279 1066
PflMI CCAn_nnn'nTGG
Cuts at: 0 1600 2460 2721 3345
Size: 1600 860 261 624
Fragments arranged by size:
1600 860 624 261
PfoI T'CCnGG_A
Cuts at: 0 140 1771 1984 3345
Size: 140 1631 213 1361
Fragments arranged by size:
1631 1361 213 140
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2893 2925 3345
Size: 2893 32 420
Fragments arranged by size:
2893 420 32
PpuMI rG'GwC_Cy
Cuts at: 0 2407 3204 3345
Size: 2407 797 141
Fragments arranged by size:
2407 797 141
PspOMI G'GGCC_C
Cuts at: 0 174 3108 3333 3345
Size: 174 2934 225 12
Fragments arranged by size:
2934 225 174 12
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1564 1596 3345
Size: 1564 32 1749
Fragments arranged by size:
1749 1564 32
PstI C_TGCA'G
Cuts at: 0 995 1565 1709 2508 3345
Size: 995 570 144 799 837
Fragments arranged by size:
995 837 799 570 144
PvuII CAG'CTG
Cuts at: 0 1917 2503 2919 3267 3345
Size: 1917 586 416 348 78
Fragments arranged by size:
1917 586 416 348 78
SacI G_AGCT'C
Cuts at: 0 1220 3345
Size: 1220 2125
SacII CC_GC'GG
Cuts at: 0 966 3345
Size: 966 2379
SalI G'TCGA_C
Cuts at: 0 2592 3345
Size: 2592 753
SanDI GG'GwC_CC
Cuts at: 0 2407 3204 3345
Size: 2407 797 141
Fragments arranged by size:
2407 797 141
ScaI AGT'ACT
Cuts at: 0 1981 3345
Size: 1981 1364
SexAI A'CCwGG_T
Cuts at: 0 3239 3345
Size: 3239 106
SfcI C'TryA_G
Cuts at: 0 991 1561 1705 2504 2623 2659 3345
Size: 991 570 144 799 119 36 686
Fragments arranged by size:
991 799 686 570 144 119 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1532 1696 3345
Size: 1532 164 1649
Fragments arranged by size:
1649 1532 164
SfoI GGC'GCC
Cuts at: 0 88 320 358 1662 3345
Size: 88 232 38 1304 1683
Fragments arranged by size:
1683 1304 232 88 38
SmaI CCC'GGG
Cuts at: 0 1318 2728 3345
Size: 1318 1410 617
Fragments arranged by size:
1410 1318 617
SmlI C'TyrA_G
Cuts at: 0 1624 1969 3345
Size: 1624 345 1376
Fragments arranged by size:
1624 1376 345
SphI G_CATG'C
Cuts at: 0 771 2578 3345
Size: 771 1807 767
Fragments arranged by size:
1807 771 767
SrfI GCCC'GGGC
Cuts at: 0 1318 3345
Size: 1318 2027
StuI AGG'CCT
Cuts at: 0 949 1792 3345
Size: 949 843 1553
Fragments arranged by size:
1553 949 843
StyI C'CwwG_G
Cuts at: 0 1052 1452 1727 1778 1787 2454 2699 3254
Size: 1052 400 275 51 9 667 245 555
Cuts at: 3254 3345
Size: 91
Fragments arranged by size:
1052 667 555 400 275 245 91 51
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 171 615 1855 3227 3345
Size: 171 444 1240 1372 118
Fragments arranged by size:
1372 1240 444 171 118
TatI w'GTAC_w
Cuts at: 0 1979 2150 2282 3345
Size: 1979 171 132 1063
Fragments arranged by size:
1979 1063 171 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 607 639 906 938 3345
Size: 607 32 267 32 2407
Fragments arranged by size:
2407 607 267 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1129 3345
Size: 1129 2216
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3036 3091 3345
Size: 3036 55 254
Fragments arranged by size:
3036 254 55
XmaI C'CCGG_G
Cuts at: 0 1316 2726 3345
Size: 1316 1410 619
Fragments arranged by size:
1410 1316 619
XmnI GAAnn'nnTTC
Cuts at: 0 388 3345
Size: 388 2957
Enzymes that do cut:
AarI AccI AflIII AleI AlfI AlwNI ApaI ApaLI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I HincII KasI MluI MscI MslI MspA1I NarI
NcoI NspI PasI PciI PflMI PfoI PpiI PpuMI
PspOMI PsrI PstI PvuII SacI SacII SalI SanDI
ScaI SexAI SfcI SfiI SfoI SmaI SmlI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AloI ApoI AscI AseI AsiSI AvrII BfrBI BmtI
BsiWI BsmI BsmBI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I DraI DraIII DrdI EagI EarI EcoRI EcoRV
FalI FseI FspAI HindIII HpaI KpnI MfeI MmeI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PmlI PshAI PsiI PspXI PvuI RsrII SapI
SbfI SgfI SgrAI SnaBI SpeI SspI SwaI XbaI
XhoI ZraI