Restriction map for
pF1KA0425
(Linear) (Six-base) MAPSORT of: KIAA0425.seq Check: 8069 from: 1 to: 3672
KIAA0425.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 165 736 3672
Size: 165 571 2936
Fragments arranged by size:
2936 571 165
AccI GT'mk_AC
Cuts at: 0 106 822 3672
Size: 106 716 2850
Fragments arranged by size:
2850 716 106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 520 881 1625 2860 3289 3665 3672
Size: 2 518 361 744 1235 429 376 7
Fragments arranged by size:
1235 744 518 429 376 361 7 2
AfeI AGC'GCT
Cuts at: 0 3517 3672
Size: 3517 155
AflIII A'CryG_T
Cuts at: 0 2648 3092 3592 3672
Size: 2648 444 500 80
Fragments arranged by size:
2648 500 444 80
AhdI GACnn_n'nnGTC
Cuts at: 0 2853 3672
Size: 2853 819
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 134 166 2036 2068 3672
Size: 134 32 1870 32 1604
Fragments arranged by size:
1870 1604 134 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1267 2439 2979 3672
Size: 1267 1172 540 693
Fragments arranged by size:
1267 1172 693 540
ApoI r'AATT_y
Cuts at: 0 864 891 1734 2045 2966 3672
Size: 864 27 843 311 921 706
Fragments arranged by size:
921 864 843 706 311 27
BanI G'GyrC_C
Cuts at: 0 1971 3672
Size: 1971 1701
BanII G_rGCy'C
Cuts at: 0 2588 3020 3672
Size: 2588 432 652
Fragments arranged by size:
2588 652 432
BbsI GAAGACnn'nnnn_
Cuts at: 0 688 1946 2129 2215 2852 3653 3672
Size: 688 1258 183 86 637 801 19
Fragments arranged by size:
1258 801 688 637 183 86 19
BbvCI CC'TCA_GC
Cuts at: 0 645 780 3672
Size: 645 135 2892
Fragments arranged by size:
2892 645 135
BclI T'GATC_A
Cuts at: 0 233 3672
Size: 233 3439
BfrBI ATG'CAT
Cuts at: 0 1433 3672
Size: 1433 2239
BlpI GC'TnA_GC
Cuts at: 0 1721 3672
Size: 1721 1951
Bme1580I G_kGCm'C
Cuts at: 0 243 908 1713 3672
Size: 243 665 805 1959
Fragments arranged by size:
1959 805 665 243
BmrI ACTGGGnnnn_n'
Cuts at: 0 237 1035 2685 3427 3672
Size: 237 798 1650 742 245
Fragments arranged by size:
1650 798 742 245 237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 1009 1661 2015 3672
Size: 55 954 652 354 1657
Fragments arranged by size:
1657 954 652 354 55
Bpu10I CC'TnA_GC
Cuts at: 0 645 780 3512 3672
Size: 645 135 2732 160
Fragments arranged by size:
2732 645 160 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2191 2762 2780 3672
Size: 2191 571 18 892
Fragments arranged by size:
2191 892 571 18
BsaI GGTCTCn'nnnn_
Cuts at: 0 1027 2907 3672
Size: 1027 1880 765
Fragments arranged by size:
1880 1027 765
BsaAI yAC'GTr
Cuts at: 0 3595 3672
Size: 3595 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1391 1421 3672
Size: 1391 30 2251
Fragments arranged by size:
2251 1391 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 164 980 3672
Size: 164 816 2692
Fragments arranged by size:
2692 816 164
BseYI C'CCAG_C
Cuts at: 0 1046 1328 2339 3138 3672
Size: 1046 282 1011 799 534
Fragments arranged by size:
1046 1011 799 534 282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1371 1778 2844 3672
Size: 1371 407 1066 828
Fragments arranged by size:
1371 1066 828 407
BsiHKAI G_wGCw'C
Cuts at: 0 3224 3672
Size: 3224 448
BsmI GAATG_Cn'
Cuts at: 0 381 1435 1933 3672
Size: 381 1054 498 1739
Fragments arranged by size:
1739 1054 498 381
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1036 3246 3672
Size: 1036 2210 426
Fragments arranged by size:
2210 1036 426
Bsp1286I G_dGCh'C
Cuts at: 0 243 908 1713 2588 3020 3224 3672
Size: 243 665 805 875 432 204 448
Fragments arranged by size:
875 805 665 448 432 243 204
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 165 736 1556 3672
Size: 165 571 820 2116
Fragments arranged by size:
2116 820 571 165
BsrBI CCG'CTC
Cuts at: 0 2828 3672
Size: 2828 844
BsrDI GCAATG_nn'
Cuts at: 0 1214 3672
Size: 1214 2458
BsrGI T'GTAC_A
Cuts at: 0 750 3672
Size: 750 2922
BstAPI GCAn_nnn'nTGC
Cuts at: 0 833 3672
Size: 833 2839
BstXI CCAn_nnnn'nTGG
Cuts at: 0 675 1054 2339 3672
Size: 675 379 1285 1333
Fragments arranged by size:
1333 1285 675 379
BstYI r'GATC_y
Cuts at: 0 640 2712 3672
Size: 640 2072 960
Fragments arranged by size:
2072 960 640
BstZ17I GTA'TAC
Cuts at: 0 823 3672
Size: 823 2849
Bsu36I CC'TnA_GG
Cuts at: 0 2766 3672
Size: 2766 906
BtgI C'CryG_G
Cuts at: 0 1625 3672
Size: 1625 2047
BtsI GCAGTG_nn'
Cuts at: 0 435 3672
Size: 435 3237
DraI TTT'AAA
Cuts at: 0 218 3167 3672
Size: 218 2949 505
Fragments arranged by size:
2949 505 218
DraIII CAC_nnn'GTG
Cuts at: 0 131 619 1069 3672
Size: 131 488 450 2603
Fragments arranged by size:
2603 488 450 131
EaeI y'GGCC_r
Cuts at: 0 1466 3672
Size: 1466 2206
EarI CTCTTCn'nnn_
Cuts at: 0 1406 2012 2195 2342 2547 2696 3107 3672
Size: 1406 606 183 147 205 149 411 565
Fragments arranged by size:
1406 606 565 411 205 183 149 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 55 520 881 1009 1625 1661 2015
Size: 2 53 465 361 128 616 36 354
Cuts at: 2015 2860 3289 3665 3672
Size: 845 429 376 7
Fragments arranged by size:
845 616 465 429 376 361 354 128
53 36 7 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 73 3672
Size: 73 3599
EcoO109I rG'GnC_Cy
Cuts at: 0 1648 2436 3672
Size: 1648 788 1236
Fragments arranged by size:
1648 1236 788
EcoRI G'AATT_C
Cuts at: 0 891 1734 2045 2966 3672
Size: 891 843 311 921 706
Fragments arranged by size:
921 891 843 706 311
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2155 3252 3672
Size: 2155 1097 420
Fragments arranged by size:
2155 1097 420
HaeII r_GCGC'y
Cuts at: 0 3146 3519 3672
Size: 3146 373 153
Fragments arranged by size:
3146 373 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 535 567 2202 2234 2296 2308 2328 2340
Size: 535 32 1635 32 62 12 20 12
Cuts at: 2340 2440 2472 2477 2509 2839 2871 3249 3276
Size: 100 32 5 32 330 32 378 27
Cuts at: 3276 3281 3308 3419 3451 3672
Size: 5 27 111 32 221
Fragments arranged by size:
1635 535 378 330 221 111 100 62
32 32 32 32 32 32 27 27
20 12 12 5 5
HincII GTy'rAC
Cuts at: 0 296 1090 3590 3672
Size: 296 794 2500 82
Fragments arranged by size:
2500 794 296 82
HindIII A'AGCT_T
Cuts at: 0 1859 2137 3229 3672
Size: 1859 278 1092 443
Fragments arranged by size:
1859 1092 443 278
HpaI GTT'AAC
Cuts at: 0 3590 3672
Size: 3590 82
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1789 3225 3462 3672
Size: 1789 1436 237 210
Fragments arranged by size:
1789 1436 237 210
MscI TGG'CCA
Cuts at: 0 1468 3672
Size: 1468 2204
MslI CAynn'nnrTG
Cuts at: 0 2528 3426 3672
Size: 2528 898 246
Fragments arranged by size:
2528 898 246
MspA1I CmG'CkG
Cuts at: 0 34 845 1018 3138 3672
Size: 34 811 173 2120 534
Fragments arranged by size:
2120 811 534 173 34
NcoI C'CATG_G
Cuts at: 0 1625 3672
Size: 1625 2047
NdeI CA'TA_TG
Cuts at: 0 300 3672
Size: 300 3372
NsiI A_TGCA'T
Cuts at: 0 1435 3672
Size: 1435 2237
NspI r_CATG'y
Cuts at: 0 366 534 1054 1437 2652 3096 3672
Size: 366 168 520 383 1215 444 576
Fragments arranged by size:
1215 576 520 444 383 366 168
PciI A'CATG_T
Cuts at: 0 2648 3092 3672
Size: 2648 444 580
Fragments arranged by size:
2648 580 444
PflMI CCAn_nnn'nTGG
Cuts at: 0 411 3672
Size: 411 3261
PfoI T'CCnGG_A
Cuts at: 0 2629 3672
Size: 2629 1043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2234 2266 3672
Size: 2234 32 1406
Fragments arranged by size:
2234 1406 32
PpuMI rG'GwC_Cy
Cuts at: 0 1648 2436 3672
Size: 1648 788 1236
Fragments arranged by size:
1648 1236 788
PsiI TTA'TAA
Cuts at: 0 1307 3672
Size: 1307 2365
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3059 3091 3672
Size: 3059 32 581
Fragments arranged by size:
3059 581 32
PstI C_TGCA'G
Cuts at: 0 747 838 1017 3672
Size: 747 91 179 2655
Fragments arranged by size:
2655 747 179 91
PvuII CAG'CTG
Cuts at: 0 34 845 1018 3138 3672
Size: 34 811 173 2120 534
Fragments arranged by size:
2120 811 534 173 34
SapI GCTCTTCn'nnn_
Cuts at: 0 2342 2696 3107 3672
Size: 2342 354 411 565
Fragments arranged by size:
2342 565 411 354
ScaI AGT'ACT
Cuts at: 0 1800 2775 3672
Size: 1800 975 897
Fragments arranged by size:
1800 975 897
SfcI C'TryA_G
Cuts at: 0 743 834 840 1013 1281 1727 2570 2817
Size: 743 91 6 173 268 446 843 247
Cuts at: 2817 3672
Size: 855
Fragments arranged by size:
855 843 743 446 268 247 173 91
6
SmlI C'TyrA_G
Cuts at: 0 2170 2777 2795 3672
Size: 2170 607 18 877
Fragments arranged by size:
2170 877 607 18
SpeI A'CTAG_T
Cuts at: 0 262 3672
Size: 262 3410
SspI AAT'ATT
Cuts at: 0 1219 1620 3672
Size: 1219 401 2052
Fragments arranged by size:
2052 1219 401
StuI AGG'CCT
Cuts at: 0 2740 3672
Size: 2740 932
StyI C'CwwG_G
Cuts at: 0 1625 1840 2283 2696 2961 3672
Size: 1625 215 443 413 265 711
Fragments arranged by size:
1625 711 443 413 265 215
TatI w'GTAC_w
Cuts at: 0 750 1798 2290 2773 3272 3672
Size: 750 1048 492 483 499 400
Fragments arranged by size:
1048 750 499 492 483 400
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1391 1423 3143 3175 3672
Size: 1391 32 1720 32 497
Fragments arranged by size:
1720 1391 497 32 32
XbaI T'CTAG_A
Cuts at: 0 2256 3672
Size: 2256 1416
XmnI GAAnn'nnTTC
Cuts at: 0 868 891 1537 1668 2138 2252 2410 2849
Size: 868 23 646 131 470 114 158 439
Cuts at: 2849 3672
Size: 823
Fragments arranged by size:
868 823 646 470 439 158 131 114
23
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AloI AlwNI
ApoI BanI BanII BbsI BbvCI BclI BfrBI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrGI BstAPI BstXI BstYI BstZ17I Bsu36I
BtgI BtsI DraI DraIII EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI
MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI
PciI PflMI PfoI PpiI PpuMI PsiI PsrI PstI
PvuII SapI ScaI SfcI SmlI SpeI SspI StuI
StyI TatI TstI XbaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AleI AlfI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BcgI BciVI BglI BglII BmgBI BmtI BplI
BsaBI BsaHI BsaWI BsiEI BsiWI BspEI BspHI BsrFI
BssHII BssSI BstBI BstEII BtgZI ClaI CspCI DrdI
EagI EciI EcoICRI EcoRV FseI FspI FspAI KasI
KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PmeI PmlI PshAI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SwaI TaqII Tth111I XcmI XhoI XmaI ZraI