Restriction map for
pF1KSDA0426
(Linear) (Six-base) MAPSORT of: KIAA0426.seq Check: 90 from: 1 to: 1812
KIAA0426.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AccI GT'mk_AC
Cuts at: 0 453 1812
Size: 453 1359
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 620 707 1335 1668 1812
Size: 129 491 87 628 333 144
Fragments arranged by size:
628 491 333 144 129 87
AhdI GACnn_n'nnGTC
Cuts at: 0 173 449 1812
Size: 173 276 1363
Fragments arranged by size:
1363 276 173
AlwNI CAG_nnn'CTG
Cuts at: 0 222 255 282 1812
Size: 222 33 27 1530
Fragments arranged by size:
1530 222 33 27
ApaLI G'TGCA_C
Cuts at: 0 1161 1812
Size: 1161 651
ApoI r'AATT_y
Cuts at: 0 579 634 1382 1466 1812
Size: 579 55 748 84 346
Fragments arranged by size:
748 579 346 84 55
BamHI G'GATC_C
Cuts at: 0 881 1812
Size: 881 931
BanI G'GyrC_C
Cuts at: 0 1228 1812
Size: 1228 584
BanII G_rGCy'C
Cuts at: 0 305 707 1771 1812
Size: 305 402 1064 41
Fragments arranged by size:
1064 402 305 41
BbeI G_GCGC'C
Cuts at: 0 1232 1812
Size: 1232 580
BbsI GAAGACnn'nnnn_
Cuts at: 0 123 628 1705 1812
Size: 123 505 1077 107
Fragments arranged by size:
1077 505 123 107
BbvCI CC'TCA_GC
Cuts at: 0 223 1812
Size: 223 1589
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 191 225 1812
Size: 191 34 1587
Fragments arranged by size:
1587 191 34
BglII A'GATC_T
Cuts at: 0 1806 1812
Size: 1806 6
Bme1580I G_kGCm'C
Cuts at: 0 1165 1812
Size: 1165 647
BmrI ACTGGGnnnn_n'
Cuts at: 0 425 467 1400 1652 1812
Size: 425 42 933 252 160
Fragments arranged by size:
933 425 252 160 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 420 1812
Size: 420 1392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 289 292 382 469 503 702 911
Size: 277 12 3 90 87 34 199 209
Cuts at: 911 995 1496 1580 1769 1812
Size: 84 501 84 189 43
Fragments arranged by size:
501 277 209 199 189 90 87 84
84 43 34 12 3
Bpu10I CC'TnA_GC
Cuts at: 0 223 1765 1812
Size: 223 1542 47
Fragments arranged by size:
1542 223 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 226 408 1812
Size: 226 182 1404
Fragments arranged by size:
1404 226 182
BsaHI Gr'CG_yC
Cuts at: 0 1229 1812
Size: 1229 583
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 386 416 1812
Size: 386 30 1396
Fragments arranged by size:
1396 386 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 313 358 1812
Size: 313 45 1454
Fragments arranged by size:
1454 313 45
BseYI C'CCAG_C
Cuts at: 0 500 1812
Size: 500 1312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1812
Size: 337 1475
BsiHKAI G_wGCw'C
Cuts at: 0 305 1165 1771 1812
Size: 305 860 606 41
Fragments arranged by size:
860 606 305 41
BsmI GAATG_Cn'
Cuts at: 0 1599 1812
Size: 1599 213
BsmBI CGTCTCn'nnnn_
Cuts at: 0 436 1812
Size: 436 1376
Bsp1286I G_dGCh'C
Cuts at: 0 305 707 1165 1771 1812
Size: 305 402 458 606 41
Fragments arranged by size:
606 458 402 305 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 390 1812
Size: 107 283 1422
Fragments arranged by size:
1422 283 107
BsrFI r'CCGG_y
Cuts at: 0 1019 1225 1812
Size: 1019 206 587
Fragments arranged by size:
1019 587 206
BssSI C'ACGA_G
Cuts at: 0 677 1812
Size: 677 1135
BstYI r'GATC_y
Cuts at: 0 881 1133 1806 1812
Size: 881 252 673 6
Fragments arranged by size:
881 673 252 6
Bsu36I CC'TnA_GG
Cuts at: 0 296 1812
Size: 296 1516
BtgI C'CryG_G
Cuts at: 0 933 1812
Size: 933 879
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1236 1271 1812
Size: 1236 35 541
Fragments arranged by size:
1236 541 35
DraI TTT'AAA
Cuts at: 0 584 1812
Size: 584 1228
DraIII CAC_nnn'GTG
Cuts at: 0 1169 1812
Size: 1169 643
EaeI y'GGCC_r
Cuts at: 0 1017 1812
Size: 1017 795
EarI CTCTTCn'nnn_
Cuts at: 0 62 695 1812
Size: 62 633 1117
Fragments arranged by size:
1117 633 62
EcoICRI GAG'CTC
Cuts at: 0 303 1769 1812
Size: 303 1466 43
Fragments arranged by size:
1466 303 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 277 289 292 382 469 503 620
Size: 129 148 12 3 90 87 34 117
Cuts at: 620 702 707 911 995 1335 1496 1580 1668
Size: 82 5 204 84 340 161 84 88
Cuts at: 1668 1769 1812
Size: 101 43
Fragments arranged by size:
340 204 161 148 129 117 101 90
88 87 84 84 82 43 34 12
5 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 295 1812
Size: 295 1517
EcoO109I rG'GnC_Cy
Cuts at: 0 96 1568 1812
Size: 96 1472 244
Fragments arranged by size:
1472 244 96
EcoRI G'AATT_C
Cuts at: 0 1382 1466 1812
Size: 1382 84 346
Fragments arranged by size:
1382 346 84
HaeII r_GCGC'y
Cuts at: 0 1232 1812
Size: 1232 580
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 159 191 386 418 435 467 786 818
Size: 159 32 195 32 17 32 319 32
Cuts at: 818 1517 1549 1812
Size: 699 32 263
Fragments arranged by size:
699 319 263 195 159 32 32 32
32 32 17
HindIII A'AGCT_T
Cuts at: 0 842 926 1812
Size: 842 84 886
Fragments arranged by size:
886 842 84
KasI G'GCGC_C
Cuts at: 0 1228 1812
Size: 1228 584
MslI CAynn'nnrTG
Cuts at: 0 1079 1812
Size: 1079 733
NarI GG'CG_CC
Cuts at: 0 1229 1812
Size: 1229 583
PflMI CCAn_nnn'nTGG
Cuts at: 0 337 1812
Size: 337 1475
PfoI T'CCnGG_A
Cuts at: 0 804 1812
Size: 804 1008
PpuMI rG'GwC_Cy
Cuts at: 0 96 1812
Size: 96 1716
PsiI TTA'TAA
Cuts at: 0 1274 1812
Size: 1274 538
SacI G_AGCT'C
Cuts at: 0 305 1771 1812
Size: 305 1466 41
Fragments arranged by size:
1466 305 41
SapI GCTCTTCn'nnn_
Cuts at: 0 695 1812
Size: 695 1117
ScaI AGT'ACT
Cuts at: 0 142 1812
Size: 142 1670
SfoI GGC'GCC
Cuts at: 0 1230 1812
Size: 1230 582
StuI AGG'CCT
Cuts at: 0 310 1115 1812
Size: 310 805 697
Fragments arranged by size:
805 697 310
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 130 303 1812
Size: 130 173 1509
Fragments arranged by size:
1509 173 130
TatI w'GTAC_w
Cuts at: 0 140 1812
Size: 140 1672
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 169 1812
Size: 169 1643
XmnI GAAnn'nnTTC
Cuts at: 0 251 1348 1597 1718 1812
Size: 251 1097 249 121 94
Fragments arranged by size:
1097 251 249 121 94
Enzymes that do cut:
AccI AcuI AhdI AlwNI ApaLI ApoI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BglII Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI BssSI
BstYI Bsu36I BtgI CspCI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HindIII
KasI MslI NarI PflMI PfoI PpuMI PsiI SacI
SapI ScaI SfoI StuI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BciVI BclI BfrBI BglI BlpI BmgBI
BmtI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I BtgZI BtsI ClaI DrdI EagI EciI
EcoRV FalI FseI FspI FspAI HincII HpaI KpnI
MfeI MluI MmeI MscI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SanDI SbfI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StyI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI