Restriction map for pF1KSDA0426
 (Linear) (Six-base) MAPSORT of: KIAA0426.seq  Check: 90  from: 1  to: 1812



KIAA0426.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AccI GT'mk_AC

Cuts at:      0     453    1812 
   Size:        453    1359
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     129     620     707    1335    1668    1812 
   Size:        129     491      87     628     333     144

  Fragments arranged by size: 

                 628     491     333     144     129      87
 
AhdI GACnn_n'nnGTC

Cuts at:      0     173     449    1812 
   Size:        173     276    1363

  Fragments arranged by size: 

                1363     276     173
 
AlwNI CAG_nnn'CTG

Cuts at:      0     222     255     282    1812 
   Size:        222      33      27    1530

  Fragments arranged by size: 

                1530     222      33      27
 
ApaLI G'TGCA_C

Cuts at:      0    1161    1812 
   Size:       1161     651
 
ApoI r'AATT_y

Cuts at:      0     579     634    1382    1466    1812 
   Size:        579      55     748      84     346

  Fragments arranged by size: 

                 748     579     346      84      55
 
BamHI G'GATC_C

Cuts at:      0     881    1812 
   Size:        881     931
 
BanI G'GyrC_C

Cuts at:      0    1228    1812 
   Size:       1228     584
 
BanII G_rGCy'C

Cuts at:      0     305     707    1771    1812 
   Size:        305     402    1064      41

  Fragments arranged by size: 

                1064     402     305      41
 
BbeI G_GCGC'C

Cuts at:      0    1232    1812 
   Size:       1232     580
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     123     628    1705    1812 
   Size:        123     505    1077     107

  Fragments arranged by size: 

                1077     505     123     107
 
BbvCI CC'TCA_GC

Cuts at:      0     223    1812 
   Size:        223    1589
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     191     225    1812 
   Size:        191      34    1587

  Fragments arranged by size: 

                1587     191      34
 
BglII A'GATC_T

Cuts at:      0    1806    1812 
   Size:       1806       6
 
Bme1580I G_kGCm'C

Cuts at:      0    1165    1812 
   Size:       1165     647
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     425     467    1400    1652    1812 
   Size:        425      42     933     252     160

  Fragments arranged by size: 

                 933     425     252     160      42
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     420    1812 
   Size:        420    1392
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     289     292     382     469     503     702     911 
   Size:        277      12       3      90      87      34     199     209

Cuts at:    911     995    1496    1580    1769    1812 
   Size:         84     501      84     189      43

  Fragments arranged by size: 

                 501     277     209     199     189      90      87      84
                  84      43      34      12       3
 
Bpu10I CC'TnA_GC

Cuts at:      0     223    1765    1812 
   Size:        223    1542      47

  Fragments arranged by size: 

                1542     223      47
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     226     408    1812 
   Size:        226     182    1404

  Fragments arranged by size: 

                1404     226     182
 
BsaHI Gr'CG_yC

Cuts at:      0    1229    1812 
   Size:       1229     583
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     386     416    1812 
   Size:        386      30    1396

  Fragments arranged by size: 

                1396     386      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     313     358    1812 
   Size:        313      45    1454

  Fragments arranged by size: 

                1454     313      45
 
BseYI C'CCAG_C

Cuts at:      0     500    1812 
   Size:        500    1312
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337    1812 
   Size:        337    1475
 
BsiHKAI G_wGCw'C

Cuts at:      0     305    1165    1771    1812 
   Size:        305     860     606      41

  Fragments arranged by size: 

                 860     606     305      41
 
BsmI GAATG_Cn'

Cuts at:      0    1599    1812 
   Size:       1599     213
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     436    1812 
   Size:        436    1376
 
Bsp1286I G_dGCh'C

Cuts at:      0     305     707    1165    1771    1812 
   Size:        305     402     458     606      41

  Fragments arranged by size: 

                 606     458     402     305      41
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     107     390    1812 
   Size:        107     283    1422

  Fragments arranged by size: 

                1422     283     107
 
BsrFI r'CCGG_y

Cuts at:      0    1019    1225    1812 
   Size:       1019     206     587

  Fragments arranged by size: 

                1019     587     206
 
BssSI C'ACGA_G

Cuts at:      0     677    1812 
   Size:        677    1135
 
BstYI r'GATC_y

Cuts at:      0     881    1133    1806    1812 
   Size:        881     252     673       6

  Fragments arranged by size: 

                 881     673     252       6
 
Bsu36I CC'TnA_GG

Cuts at:      0     296    1812 
   Size:        296    1516
 
BtgI C'CryG_G

Cuts at:      0     933    1812 
   Size:        933     879
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1236    1271    1812 
   Size:       1236      35     541

  Fragments arranged by size: 

                1236     541      35
 
DraI TTT'AAA

Cuts at:      0     584    1812 
   Size:        584    1228
 
DraIII CAC_nnn'GTG

Cuts at:      0    1169    1812 
   Size:       1169     643
 
EaeI y'GGCC_r

Cuts at:      0    1017    1812 
   Size:       1017     795
 
EarI CTCTTCn'nnn_

Cuts at:      0      62     695    1812 
   Size:         62     633    1117

  Fragments arranged by size: 

                1117     633      62
 
EcoICRI GAG'CTC

Cuts at:      0     303    1769    1812 
   Size:        303    1466      43

  Fragments arranged by size: 

                1466     303      43
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     129     277     289     292     382     469     503     620 
   Size:        129     148      12       3      90      87      34     117

Cuts at:    620     702     707     911     995    1335    1496    1580    1668 
   Size:         82       5     204      84     340     161      84      88

Cuts at:   1668    1769    1812 
   Size:        101      43

  Fragments arranged by size: 

                 340     204     161     148     129     117     101      90
                  88      87      84      84      82      43      34      12
                   5       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     295    1812 
   Size:        295    1517
 
EcoO109I rG'GnC_Cy

Cuts at:      0      96    1568    1812 
   Size:         96    1472     244

  Fragments arranged by size: 

                1472     244      96
 
EcoRI G'AATT_C

Cuts at:      0    1382    1466    1812 
   Size:       1382      84     346

  Fragments arranged by size: 

                1382     346      84
 
HaeII r_GCGC'y

Cuts at:      0    1232    1812 
   Size:       1232     580
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     159     191     386     418     435     467     786     818 
   Size:        159      32     195      32      17      32     319      32

Cuts at:    818    1517    1549    1812 
   Size:        699      32     263

  Fragments arranged by size: 

                 699     319     263     195     159      32      32      32
                  32      32      17
 
HindIII A'AGCT_T

Cuts at:      0     842     926    1812 
   Size:        842      84     886

  Fragments arranged by size: 

                 886     842      84
 
KasI G'GCGC_C

Cuts at:      0    1228    1812 
   Size:       1228     584
 
MslI CAynn'nnrTG

Cuts at:      0    1079    1812 
   Size:       1079     733
 
NarI GG'CG_CC

Cuts at:      0    1229    1812 
   Size:       1229     583
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     337    1812 
   Size:        337    1475
 
PfoI T'CCnGG_A

Cuts at:      0     804    1812 
   Size:        804    1008
 
PpuMI rG'GwC_Cy

Cuts at:      0      96    1812 
   Size:         96    1716
 
PsiI TTA'TAA

Cuts at:      0    1274    1812 
   Size:       1274     538
 
SacI G_AGCT'C

Cuts at:      0     305    1771    1812 
   Size:        305    1466      41

  Fragments arranged by size: 

                1466     305      41
 
SapI GCTCTTCn'nnn_

Cuts at:      0     695    1812 
   Size:        695    1117
 
ScaI AGT'ACT

Cuts at:      0     142    1812 
   Size:        142    1670
 
SfoI GGC'GCC

Cuts at:      0    1230    1812 
   Size:       1230     582
 
StuI AGG'CCT

Cuts at:      0     310    1115    1812 
   Size:        310     805     697

  Fragments arranged by size: 

                 805     697     310
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     130     303    1812 
   Size:        130     173    1509

  Fragments arranged by size: 

                1509     173     130
 
TatI w'GTAC_w

Cuts at:      0     140    1812 
   Size:        140    1672
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     169    1812 
   Size:        169    1643
 
XmnI GAAnn'nnTTC

Cuts at:      0     251    1348    1597    1718    1812 
   Size:        251    1097     249     121      94

  Fragments arranged by size: 

                1097     251     249     121      94
 

 Enzymes that do cut:

     AccI     AcuI     AhdI    AlwNI    ApaLI     ApoI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BglII Bme1580I     BmrI
     BplI     BpmI   Bpu10I     BsaI    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrFI    BssSI
    BstYI   Bsu36I     BtgI    CspCI     DraI   DraIII     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I  HindIII
     KasI     MslI     NarI    PflMI     PfoI    PpuMI     PsiI     SacI
     SapI     ScaI     SfoI     StuI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApaI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI
     BmtI    BpuEI    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI    BspEI
    BspHI    BsrBI    BsrDI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I    BtgZI     BtsI     ClaI     DrdI     EagI     EciI
    EcoRV     FalI     FseI     FspI    FspAI   HincII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SbfI
    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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