Restriction map for
pF1KSDA0427
(Linear) (Six-base) MAPSORT of: KIAA0427.seq Check: 9622 from: 1 to: 1794
KIAA0427.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1522 1794
Size: 1522 272
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 1794
Size: 306 1488
AfeI AGC'GCT
Cuts at: 0 76 1417 1794
Size: 76 1341 377
Fragments arranged by size:
1341 377 76
AlwNI CAG_nnn'CTG
Cuts at: 0 117 215 408 1785 1794
Size: 117 98 193 1377 9
Fragments arranged by size:
1377 193 117 98 9
AvaI C'yCGr_G
Cuts at: 0 227 880 991 1794
Size: 227 653 111 803
Fragments arranged by size:
803 653 227 111
BanI G'GyrC_C
Cuts at: 0 328 749 1456 1794
Size: 328 421 707 338
Fragments arranged by size:
707 421 338 328
BanII G_rGCy'C
Cuts at: 0 240 691 1484 1529 1637 1718 1794
Size: 240 451 793 45 108 81 76
Fragments arranged by size:
793 451 240 108 81 76 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 1025 1443 1794
Size: 1025 418 351
Fragments arranged by size:
1025 418 351
BglI GCCn_nnn'nGGC
Cuts at: 0 916 1794
Size: 916 878
BlpI GC'TnA_GC
Cuts at: 0 690 1794
Size: 690 1104
Bme1580I G_kGCm'C
Cuts at: 0 289 872 1504 1669 1794
Size: 289 583 632 165 125
Fragments arranged by size:
632 583 289 165 125
BmgBI CAC'GTC
Cuts at: 0 1164 1410 1794
Size: 1164 246 384
Fragments arranged by size:
1164 384 246
BmrI ACTGGGnnnn_n'
Cuts at: 0 156 171 363 1794
Size: 156 15 192 1431
Fragments arranged by size:
1431 192 156 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 734 1794
Size: 734 1060
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 118 865 1012 1624 1794
Size: 91 27 747 147 612 170
Fragments arranged by size:
747 612 170 147 91 27
BsaI GGTCTCn'nnnn_
Cuts at: 0 588 811 1794
Size: 588 223 983
Fragments arranged by size:
983 588 223
BsaAI yAC'GTr
Cuts at: 0 93 1794
Size: 93 1701
BsaBI GATnn'nnATC
Cuts at: 0 1129 1794
Size: 1129 665
BsaHI Gr'CG_yC
Cuts at: 0 1744 1794
Size: 1744 50
BsaWI w'CCGG_w
Cuts at: 0 1343 1794
Size: 1343 451
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1080 1110 1794
Size: 1080 30 684
Fragments arranged by size:
1080 684 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 79 582 1207 1234 1345 1405 1794
Size: 18 61 503 625 27 111 60 389
Fragments arranged by size:
625 503 389 111 61 60 27 18
BseYI C'CCAG_C
Cuts at: 0 282 1100 1227 1422 1794
Size: 282 818 127 195 372
Fragments arranged by size:
818 372 282 195 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 1794
Size: 130 1664
BsiEI CG_ry'CG
Cuts at: 0 423 1272 1693 1794
Size: 423 849 421 101
Fragments arranged by size:
849 423 421 101
BsiHKAI G_wGCw'C
Cuts at: 0 1496 1529 1637 1718 1794
Size: 1496 33 108 81 76
Fragments arranged by size:
1496 108 81 76 33
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1081 1794
Size: 1081 713
Bsp1286I G_dGCh'C
Cuts at: 0 240 289 691 872 1484 1496 1504 1529
Size: 240 49 402 181 612 12 8 25
Cuts at: 1529 1637 1669 1718 1794
Size: 108 32 49 76
Fragments arranged by size:
612 402 240 181 108 76 49 49
32 25 12 8
BspEI T'CCGG_A
Cuts at: 0 1343 1794
Size: 1343 451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 350 1522 1794
Size: 350 1172 272
Fragments arranged by size:
1172 350 272
BsrBI CCG'CTC
Cuts at: 0 50 927 1008 1794
Size: 50 877 81 786
Fragments arranged by size:
877 786 81 50
BsrDI GCAATG_nn'
Cuts at: 0 813 1794
Size: 813 981
BsrFI r'CCGG_y
Cuts at: 0 740 1595 1794
Size: 740 855 199
Fragments arranged by size:
855 740 199
BssSI C'ACGA_G
Cuts at: 0 1495 1794
Size: 1495 299
BstAPI GCAn_nnn'nTGC
Cuts at: 0 117 408 1794
Size: 117 291 1386
Fragments arranged by size:
1386 291 117
BstEII G'GTnAC_C
Cuts at: 0 628 1794
Size: 628 1166
BstXI CCAn_nnnn'nTGG
Cuts at: 0 625 1188 1794
Size: 625 563 606
Fragments arranged by size:
625 606 563
BstYI r'GATC_y
Cuts at: 0 1130 1794
Size: 1130 664
Bsu36I CC'TnA_GG
Cuts at: 0 705 1519 1794
Size: 705 814 275
Fragments arranged by size:
814 705 275
BtgI C'CryG_G
Cuts at: 0 723 753 1082 1794
Size: 723 30 329 712
Fragments arranged by size:
723 712 329 30
BtsI GCAGTG_nn'
Cuts at: 0 651 851 1794
Size: 651 200 943
Fragments arranged by size:
943 651 200
EaeI y'GGCC_r
Cuts at: 0 302 1079 1640 1794
Size: 302 777 561 154
Fragments arranged by size:
777 561 302 154
EcoICRI GAG'CTC
Cuts at: 0 1527 1635 1716 1794
Size: 1527 108 81 78
Fragments arranged by size:
1527 108 81 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 118 306 865 1012 1624 1794
Size: 91 27 188 559 147 612 170
Fragments arranged by size:
612 559 188 170 147 91 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 708 1444 1518 1794
Size: 708 736 74 276
Fragments arranged by size:
736 708 276 74
EcoO109I rG'GnC_Cy
Cuts at: 0 158 339 489 885 1794
Size: 158 181 150 396 909
Fragments arranged by size:
909 396 181 158 150
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 378 1794
Size: 378 1416
FspI TGC'GCA
Cuts at: 0 1243 1465 1794
Size: 1243 222 329
Fragments arranged by size:
1243 329 222
FspAI rTGC'GCAy
Cuts at: 0 1243 1794
Size: 1243 551
HaeII r_GCGC'y
Cuts at: 0 78 747 1318 1419 1794
Size: 78 669 571 101 375
Fragments arranged by size:
669 571 375 101 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 561 593 1080 1112 1794
Size: 561 32 487 32 682
Fragments arranged by size:
682 561 487 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 1292 1794
Size: 411 881 502
Fragments arranged by size:
881 502 411
MscI TGG'CCA
Cuts at: 0 1642 1794
Size: 1642 152
MslI CAynn'nnrTG
Cuts at: 0 806 1186 1794
Size: 806 380 608
Fragments arranged by size:
806 608 380
MspA1I CmG'CkG
Cuts at: 0 187 204 215 1291 1614 1730 1794
Size: 187 17 11 1076 323 116 64
Fragments arranged by size:
1076 323 187 116 64 17 11
NaeI GCC'GGC
Cuts at: 0 1597 1794
Size: 1597 197
NcoI C'CATG_G
Cuts at: 0 723 753 1794
Size: 723 30 1041
Fragments arranged by size:
1041 723 30
NgoMIV G'CCGG_C
Cuts at: 0 1595 1794
Size: 1595 199
NspI r_CATG'y
Cuts at: 0 282 1146 1365 1794
Size: 282 864 219 429
Fragments arranged by size:
864 429 282 219
PflMI CCAn_nnn'nTGG
Cuts at: 0 531 1794
Size: 531 1263
PpuMI rG'GwC_Cy
Cuts at: 0 158 339 489 1794
Size: 158 181 150 1305
Fragments arranged by size:
1305 181 158 150
PspXI vC'TCGA_Gb
Cuts at: 0 880 1794
Size: 880 914
PstI C_TGCA'G
Cuts at: 0 18 196 347 858 1370 1400 1586 1794
Size: 18 178 151 511 512 30 186 208
Fragments arranged by size:
512 511 208 186 178 151 30 18
PvuII CAG'CTG
Cuts at: 0 215 1614 1730 1794
Size: 215 1399 116 64
Fragments arranged by size:
1399 215 116 64
SacI G_AGCT'C
Cuts at: 0 1529 1637 1718 1794
Size: 1529 108 81 76
Fragments arranged by size:
1529 108 81 76
ScaI AGT'ACT
Cuts at: 0 1762 1794
Size: 1762 32
SexAI A'CCwGG_T
Cuts at: 0 104 1794
Size: 104 1690
SfcI C'TryA_G
Cuts at: 0 14 192 343 729 854 1366 1396 1582
Size: 14 178 151 386 125 512 30 186
Cuts at: 1582 1794
Size: 212
Fragments arranged by size:
512 386 212 186 178 151 125 30
14
SmlI C'TyrA_G
Cuts at: 0 880 1794
Size: 880 914
SphI G_CATG'C
Cuts at: 0 1146 1794
Size: 1146 648
StyI C'CwwG_G
Cuts at: 0 723 753 1794
Size: 723 30 1041
Fragments arranged by size:
1041 723 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 382 785 1794
Size: 382 403 1009
Fragments arranged by size:
1009 403 382
TatI w'GTAC_w
Cuts at: 0 1588 1760 1794
Size: 1588 172 34
Fragments arranged by size:
1588 172 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 658 690 1794
Size: 658 32 1104
Fragments arranged by size:
1104 658 32
Tth111I GACn'n_nGTC
Cuts at: 0 162 1689 1794
Size: 162 1527 105
Fragments arranged by size:
1527 162 105
XhoI C'TCGA_G
Cuts at: 0 880 1794
Size: 880 914
XmnI GAAnn'nnTTC
Cuts at: 0 556 1375 1794
Size: 556 819 419
Fragments arranged by size:
819 556 419
Enzymes that do cut:
AarI AcuI AfeI AlwNI AvaI BanI BanII BbsI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI EaeI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
FspAI HaeII Hin4I MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PflMI PpuMI PspXI PstI PvuII
SacI ScaI SexAI SfcI SmlI SphI StyI TaqII
TatI TstI Tth111I XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BmtI Bpu10I BpuEI BsiWI BsmI
BspHI BsrGI BssHII BstBI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoRI EcoRV
FseI HincII HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI XbaI XcmI XmaI ZraI