Restriction map for
pF1KA0428
(Linear) (Six-base) MAPSORT of: KIAA0428.seq Check: 1026 from: 1 to: 1110
KIAA0428.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 505 514 655 991 1110
Size: 505 9 141 336 119
Fragments arranged by size:
505 336 141 119 9
ApaLI G'TGCA_C
Cuts at: 0 970 1110
Size: 970 140
ApoI r'AATT_y
Cuts at: 0 103 809 1110
Size: 103 706 301
Fragments arranged by size:
706 301 103
AseI AT'TA_AT
Cuts at: 0 526 1110
Size: 526 584
AvaI C'yCGr_G
Cuts at: 0 689 1110
Size: 689 421
BanI G'GyrC_C
Cuts at: 0 325 868 1110
Size: 325 543 242
Fragments arranged by size:
543 325 242
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 98 132 1110
Size: 98 34 978
Fragments arranged by size:
978 98 34
BfrBI ATG'CAT
Cuts at: 0 382 1110
Size: 382 728
BglI GCCn_nnn'nGGC
Cuts at: 0 286 1110
Size: 286 824
BglII A'GATC_T
Cuts at: 0 443 1110
Size: 443 667
BlpI GC'TnA_GC
Cuts at: 0 369 1110
Size: 369 741
Bme1580I G_kGCm'C
Cuts at: 0 330 974 1110
Size: 330 644 136
Fragments arranged by size:
644 330 136
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 704 866 1110
Size: 704 162 244
Fragments arranged by size:
704 244 162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 1110
Size: 168 942
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 800 1110
Size: 565 235 310
Fragments arranged by size:
565 310 235
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 599 1110
Size: 599 511
BseYI C'CCAG_C
Cuts at: 0 290 1110
Size: 290 820
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 500 704 761 1110
Size: 440 60 204 57 349
Fragments arranged by size:
440 349 204 60 57
BsiHKAI G_wGCw'C
Cuts at: 0 974 1110
Size: 974 136
Bsp1286I G_dGCh'C
Cuts at: 0 330 974 1110
Size: 330 644 136
Fragments arranged by size:
644 330 136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 504 1110
Size: 504 606
BstAPI GCAn_nnn'nTGC
Cuts at: 0 505 514 784 1110
Size: 505 9 270 326
Fragments arranged by size:
505 326 270 9
BstBI TT'CG_AA
Cuts at: 0 118 1110
Size: 118 992
BstYI r'GATC_y
Cuts at: 0 443 1110
Size: 443 667
BtgI C'CryG_G
Cuts at: 0 803 965 1110
Size: 803 162 145
Fragments arranged by size:
803 162 145
DraI TTT'AAA
Cuts at: 0 224 1110
Size: 224 886
DraIII CAC_nnn'GTG
Cuts at: 0 659 1110
Size: 659 451
DrdI GACnn_nn'nnGTC
Cuts at: 0 649 1110
Size: 649 461
EaeI y'GGCC_r
Cuts at: 0 85 278 1110
Size: 85 193 832
Fragments arranged by size:
832 193 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 1110
Size: 168 942
EcoO109I rG'GnC_Cy
Cuts at: 0 412 1110
Size: 412 698
EcoRI G'AATT_C
Cuts at: 0 809 1110
Size: 809 301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 841 1110
Size: 841 269
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 134 166 1110
Size: 134 32 944
Fragments arranged by size:
944 134 32
HincII GTy'rAC
Cuts at: 0 762 1110
Size: 762 348
HindIII A'AGCT_T
Cuts at: 0 366 1110
Size: 366 744
MfeI C'AATT_G
Cuts at: 0 573 1110
Size: 573 537
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 1110
Size: 215 895
MscI TGG'CCA
Cuts at: 0 280 1110
Size: 280 830
MslI CAynn'nnrTG
Cuts at: 0 280 1094 1110
Size: 280 814 16
Fragments arranged by size:
814 280 16
MspA1I CmG'CkG
Cuts at: 0 502 511 775 799 1110
Size: 502 9 264 24 311
Fragments arranged by size:
502 311 264 24 9
NcoI C'CATG_G
Cuts at: 0 803 965 1110
Size: 803 162 145
Fragments arranged by size:
803 162 145
NsiI A_TGCA'T
Cuts at: 0 384 1110
Size: 384 726
NspI r_CATG'y
Cuts at: 0 927 1110
Size: 927 183
PpuMI rG'GwC_Cy
Cuts at: 0 412 1110
Size: 412 698
PstI C_TGCA'G
Cuts at: 0 501 510 774 789 1110
Size: 501 9 264 15 321
Fragments arranged by size:
501 321 264 15 9
PvuII CAG'CTG
Cuts at: 0 502 511 775 799 1110
Size: 502 9 264 24 311
Fragments arranged by size:
502 311 264 24 9
SexAI A'CCwGG_T
Cuts at: 0 755 1110
Size: 755 355
SfcI C'TryA_G
Cuts at: 0 497 506 770 785 1110
Size: 497 9 264 15 325
Fragments arranged by size:
497 325 264 15 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 286 1110
Size: 286 824
SmlI C'TyrA_G
Cuts at: 0 544 815 1110
Size: 544 271 295
Fragments arranged by size:
544 295 271
StuI AGG'CCT
Cuts at: 0 846 1110
Size: 846 264
StyI C'CwwG_G
Cuts at: 0 803 965 1110
Size: 803 162 145
Fragments arranged by size:
803 162 145
TatI w'GTAC_w
Cuts at: 0 822 1110
Size: 822 288
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 468 500 1008 1040 1110
Size: 468 32 508 32 70
Fragments arranged by size:
508 468 70 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 136 1110
Size: 136 974
Enzymes that do cut:
AlwNI ApaLI ApoI AseI AvaI BanI BcgI BfrBI
BglI BglII BlpI Bme1580I BplI BpmI BpuEI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BstAPI BstBI BstYI
BtgI DraI DraIII DrdI EaeI Eco57MI EcoO109I EcoRI
FalI Hin4I HincII HindIII MfeI MmeI MscI MslI
MspA1I NcoI NsiI NspI PpuMI PstI PvuII SexAI
SfcI SfiI SmlI StuI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI AscI
AsiSI AvrII BaeI BamHI BanII BbeI BbsI BbvCI
BciVI BclI BmgBI BmrI BmtI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
EagI EarI EciI EcoICRI EcoNI EcoRV FseI FspI
FspAI HaeII HpaI KasI KpnI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI
XhoI XmaI XmnI ZraI