Restriction map for
pF1KA0431
(Linear) (Six-base) MAPSORT of: KIAA0431.seq Check: 3435 from: 1 to: 2001
KIAA0431.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 1505 1778 2001
Size: 302 1203 273 223
Fragments arranged by size:
1203 302 273 223
AflII C'TTAA_G
Cuts at: 0 616 2001
Size: 616 1385
AflIII A'CryG_T
Cuts at: 0 734 736 2001
Size: 734 2 1265
Fragments arranged by size:
1265 734 2
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1930 1962 2001
Size: 1930 32 39
Fragments arranged by size:
1930 39 32
AlwNI CAG_nnn'CTG
Cuts at: 0 550 2001
Size: 550 1451
ApaLI G'TGCA_C
Cuts at: 0 108 230 553 1238 2001
Size: 108 122 323 685 763
Fragments arranged by size:
763 685 323 122 108
ApoI r'AATT_y
Cuts at: 0 1230 1602 2001
Size: 1230 372 399
Fragments arranged by size:
1230 399 372
BanII G_rGCy'C
Cuts at: 0 538 2001
Size: 538 1463
BbsI GAAGACnn'nnnn_
Cuts at: 0 353 2001
Size: 353 1648
BbvCI CC'TCA_GC
Cuts at: 0 501 2001
Size: 501 1500
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 163 197 2001
Size: 163 34 1804
Fragments arranged by size:
1804 163 34
BclI T'GATC_A
Cuts at: 0 528 2001
Size: 528 1473
BfrBI ATG'CAT
Cuts at: 0 9 964 2001
Size: 9 955 1037
Fragments arranged by size:
1037 955 9
BglI GCCn_nnn'nGGC
Cuts at: 0 547 2001
Size: 547 1454
BglII A'GATC_T
Cuts at: 0 777 2001
Size: 777 1224
Bme1580I G_kGCm'C
Cuts at: 0 112 173 234 557 1242 2001
Size: 112 61 61 323 685 759
Fragments arranged by size:
759 685 323 112 61 61
BmrI ACTGGGnnnn_n'
Cuts at: 0 176 2001
Size: 176 1825
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 419 627 647 2001
Size: 419 208 20 1354
Fragments arranged by size:
1354 419 208 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 984 1695 2001
Size: 984 711 306
Fragments arranged by size:
984 711 306
Bpu10I CC'TnA_GC
Cuts at: 0 501 2001
Size: 501 1500
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 863 1642 2001
Size: 287 576 779 359
Fragments arranged by size:
779 576 359 287
BsaI GGTCTCn'nnnn_
Cuts at: 0 1002 1741 2001
Size: 1002 739 260
Fragments arranged by size:
1002 739 260
BsaAI yAC'GTr
Cuts at: 0 737 2001
Size: 737 1264
BsaWI w'CCGG_w
Cuts at: 0 104 2001
Size: 104 1897
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1930 1960 1975 2001
Size: 1930 30 15 26
Fragments arranged by size:
1930 30 26 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 489 2001
Size: 489 1512
BseYI C'CCAG_C
Cuts at: 0 506 922 1397 1413 2001
Size: 506 416 475 16 588
Fragments arranged by size:
588 506 475 416 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 790 797 1202 2001
Size: 60 730 7 405 799
Fragments arranged by size:
799 730 405 60 7
BsiHKAI G_wGCw'C
Cuts at: 0 112 234 557 1242 2001
Size: 112 122 323 685 759
Fragments arranged by size:
759 685 323 122 112
BsiWI C'GTAC_G
Cuts at: 0 50 2001
Size: 50 1951
Bsp1286I G_dGCh'C
Cuts at: 0 112 173 234 538 557 1242 2001
Size: 112 61 61 304 19 685 759
Fragments arranged by size:
759 685 304 112 61 61 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 189 1794 2001
Size: 189 1605 207
Fragments arranged by size:
1605 207 189
BsrDI GCAATG_nn'
Cuts at: 0 1901 2001
Size: 1901 100
BsrGI T'GTAC_A
Cuts at: 0 1047 2001
Size: 1047 954
BssSI C'ACGA_G
Cuts at: 0 172 2001
Size: 172 1829
BstAPI GCAn_nnn'nTGC
Cuts at: 0 561 2001
Size: 561 1440
BstXI CCAn_nnnn'nTGG
Cuts at: 0 991 2001
Size: 991 1010
BstYI r'GATC_y
Cuts at: 0 777 2001
Size: 777 1224
BtsI GCAGTG_nn'
Cuts at: 0 142 824 1769 2001
Size: 142 682 945 232
Fragments arranged by size:
945 682 232 142
ClaI AT'CG_AT
Cuts at: 0 1433 2001
Size: 1433 568
DraI TTT'AAA
Cuts at: 0 1196 2001
Size: 1196 805
DrdI GACnn_nn'nnGTC
Cuts at: 0 780 2001
Size: 780 1221
EarI CTCTTCn'nnn_
Cuts at: 0 893 1442 1481 2001
Size: 893 549 39 520
Fragments arranged by size:
893 549 520 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 984 1505 1695 1778 2001
Size: 302 682 521 190 83 223
Fragments arranged by size:
682 521 302 223 190 83
EcoO109I rG'GnC_Cy
Cuts at: 0 66 195 2001
Size: 66 129 1806
Fragments arranged by size:
1806 129 66
EcoRV GAT'ATC
Cuts at: 0 400 1443 2001
Size: 400 1043 558
Fragments arranged by size:
1043 558 400
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 904 2001
Size: 904 1097
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 520 552 1014 1046 1143 1175 1182 1214
Size: 520 32 462 32 97 32 7 32
Cuts at: 1214 1263 1295 1338 1370 1926 1958 2001
Size: 49 32 43 32 556 32 43
Fragments arranged by size:
556 520 462 97 49 43 43 32
32 32 32 32 32 32 7
HindIII A'AGCT_T
Cuts at: 0 314 2001
Size: 314 1687
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 1057 2001
Size: 275 782 944
Fragments arranged by size:
944 782 275
NsiI A_TGCA'T
Cuts at: 0 11 966 2001
Size: 11 955 1035
Fragments arranged by size:
1035 955 11
NspI r_CATG'y
Cuts at: 0 13 82 116 2001
Size: 13 69 34 1885
Fragments arranged by size:
1885 69 34 13
PasI CC'CwG_GG
Cuts at: 0 1854 2001
Size: 1854 147
PflMI CCAn_nnn'nTGG
Cuts at: 0 513 1350 2001
Size: 513 837 651
Fragments arranged by size:
837 651 513
PfoI T'CCnGG_A
Cuts at: 0 1383 2001
Size: 1383 618
PmlI CAC'GTG
Cuts at: 0 737 2001
Size: 737 1264
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1930 1962 2001
Size: 1930 32 39
Fragments arranged by size:
1930 39 32
PpuMI rG'GwC_Cy
Cuts at: 0 66 195 2001
Size: 66 129 1806
Fragments arranged by size:
1806 129 66
PsiI TTA'TAA
Cuts at: 0 1129 1228 2001
Size: 1129 99 773
Fragments arranged by size:
1129 773 99
PstI C_TGCA'G
Cuts at: 0 149 186 2001
Size: 149 37 1815
Fragments arranged by size:
1815 149 37
SanDI GG'GwC_CC
Cuts at: 0 195 2001
Size: 195 1806
ScaI AGT'ACT
Cuts at: 0 675 1728 2001
Size: 675 1053 273
Fragments arranged by size:
1053 675 273
SfcI C'TryA_G
Cuts at: 0 145 182 488 944 1115 1955 2001
Size: 145 37 306 456 171 840 46
Fragments arranged by size:
840 456 306 171 145 46 37
SmlI C'TyrA_G
Cuts at: 0 302 616 878 1621 2001
Size: 302 314 262 743 380
Fragments arranged by size:
743 380 314 302 262
SphI G_CATG'C
Cuts at: 0 13 2001
Size: 13 1988
StuI AGG'CCT
Cuts at: 0 1619 2001
Size: 1619 382
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1411 1580 2001
Size: 1411 169 421
Fragments arranged by size:
1411 421 169
TatI w'GTAC_w
Cuts at: 0 673 1047 1726 1816 2001
Size: 673 374 679 90 185
Fragments arranged by size:
679 673 374 185 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1975 2001
Size: 1975 26
XbaI T'CTAG_A
Cuts at: 0 1791 2001
Size: 1791 210
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 435 1405 2001
Size: 435 970 596
Fragments arranged by size:
970 596 435
Enzymes that do cut:
AcuI AflII AflIII AloI AlwNI ApaLI ApoI BanII
BbsI BbvCI BcgI BclI BfrBI BglI BglII Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsiWI Bsp1286I BspMI
BsrDI BsrGI BssSI BstAPI BstXI BstYI BtsI ClaI
DraI DrdI EarI Eco57MI EcoO109I EcoRV FalI Hin4I
HindIII MmeI NsiI NspI PasI PflMI PfoI PmlI
PpiI PpuMI PsiI PstI SanDI ScaI SfcI SmlI
SphI StuI TaqII TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BciVI BlpI BmgBI BmtI
BsaBI BsaHI BsiEI BsmI BsmBI BspEI BspHI BsrBI
BsrFI BssHII BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
CspCI DraIII EaeI EagI EciI EcoICRI EcoNI EcoRI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PciI PmeI
PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StyI SwaI
Tth111I XhoI XmaI XmnI ZraI