Restriction map for
pF1KA0439
(Linear) (Six-base) MAPSORT of: KIAA0439.seq Check: 7264 from: 1 to: 2502
KIAA0439.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AclI AA'CG_TT
Cuts at: 0 1267 2502
Size: 1267 1235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 725 1371 1639 2502
Size: 565 160 646 268 863
Fragments arranged by size:
863 646 565 268 160
AflIII A'CryG_T
Cuts at: 0 320 2502
Size: 320 2182
AhdI GACnn_n'nnGTC
Cuts at: 0 1501 2502
Size: 1501 1001
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1882 1914 2502
Size: 1882 32 588
Fragments arranged by size:
1882 588 32
AlwNI CAG_nnn'CTG
Cuts at: 0 555 2502
Size: 555 1947
ApoI r'AATT_y
Cuts at: 0 1168 1247 1363 2099 2502
Size: 1168 79 116 736 403
Fragments arranged by size:
1168 736 403 116 79
AvaI C'yCGr_G
Cuts at: 0 56 226 418 445 2502
Size: 56 170 192 27 2057
Fragments arranged by size:
2057 192 170 56 27
BamHI G'GATC_C
Cuts at: 0 1862 2502
Size: 1862 640
BanI G'GyrC_C
Cuts at: 0 955 2502
Size: 955 1547
BanII G_rGCy'C
Cuts at: 0 419 425 2394 2502
Size: 419 6 1969 108
Fragments arranged by size:
1969 419 108 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 413 1313 2502
Size: 413 900 1189
Fragments arranged by size:
1189 900 413
BbvCI CC'TCA_GC
Cuts at: 0 916 2342 2502
Size: 916 1426 160
Fragments arranged by size:
1426 916 160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 658 692 2502
Size: 658 34 1810
Fragments arranged by size:
1810 658 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2272 2502
Size: 2272 230
BclI T'GATC_A
Cuts at: 0 1125 1278 2502
Size: 1125 153 1224
Fragments arranged by size:
1224 1125 153
BmgBI CAC'GTC
Cuts at: 0 321 2502
Size: 321 2181
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 511 2502
Size: 511 1991
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 575 970 1341 1888 2140 2184 2502
Size: 506 69 395 371 547 252 44 318
Fragments arranged by size:
547 506 395 371 318 252 69 44
Bpu10I CC'TnA_GC
Cuts at: 0 767 916 2342 2502
Size: 767 149 1426 160
Fragments arranged by size:
1426 767 160 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 2465 2502
Size: 627 1838 37
Fragments arranged by size:
1838 627 37
BsaI GGTCTCn'nnnn_
Cuts at: 0 451 2502
Size: 451 2051
BsaHI Gr'CG_yC
Cuts at: 0 2249 2502
Size: 2249 253
BsaWI w'CCGG_w
Cuts at: 0 284 853 2264 2502
Size: 284 569 1411 238
Fragments arranged by size:
1411 569 284 238
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 200 203 206 630 2502
Size: 22 178 3 3 424 1872
Fragments arranged by size:
1872 424 178 22 3 3
BseYI C'CCAG_C
Cuts at: 0 746 1082 1235 1326 2502
Size: 746 336 153 91 1176
Fragments arranged by size:
1176 746 336 153 91
BsiEI CG_ry'CG
Cuts at: 0 1110 2502
Size: 1110 1392
BsiHKAI G_wGCw'C
Cuts at: 0 2394 2502
Size: 2394 108
BsiWI C'GTAC_G
Cuts at: 0 972 2502
Size: 972 1530
BsmI GAATG_Cn'
Cuts at: 0 2172 2502
Size: 2172 330
Bsp1286I G_dGCh'C
Cuts at: 0 419 425 2394 2502
Size: 419 6 1969 108
Fragments arranged by size:
1969 419 108 6
BspEI T'CCGG_A
Cuts at: 0 853 2502
Size: 853 1649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1416 2502
Size: 1416 1086
BsrBI CCG'CTC
Cuts at: 0 386 2502
Size: 386 2116
BsrDI GCAATG_nn'
Cuts at: 0 2368 2502
Size: 2368 134
BsrFI r'CCGG_y
Cuts at: 0 374 519 902 1109 2502
Size: 374 145 383 207 1393
Fragments arranged by size:
1393 383 374 207 145
BsrGI T'GTAC_A
Cuts at: 0 717 2502
Size: 717 1785
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1082 2502
Size: 1082 1420
BstYI r'GATC_y
Cuts at: 0 532 896 1155 1862 2502
Size: 532 364 259 707 640
Fragments arranged by size:
707 640 532 364 259
Bsu36I CC'TnA_GG
Cuts at: 0 548 2502
Size: 548 1954
BtgI C'CryG_G
Cuts at: 0 1637 2468 2502
Size: 1637 831 34
Fragments arranged by size:
1637 831 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2276 2502
Size: 2276 226
BtsI GCAGTG_nn'
Cuts at: 0 666 2502
Size: 666 1836
DraI TTT'AAA
Cuts at: 0 1202 2502
Size: 1202 1300
DraIII CAC_nnn'GTG
Cuts at: 0 296 2502
Size: 296 2206
DrdI GACnn_nn'nnGTC
Cuts at: 0 44 2502
Size: 44 2458
EaeI y'GGCC_r
Cuts at: 0 1107 1260 1565 1733 2465 2502
Size: 1107 153 305 168 732 37
Fragments arranged by size:
1107 732 305 168 153 37
EagI C'GGCC_G
Cuts at: 0 1107 2502
Size: 1107 1395
EarI CTCTTCn'nnn_
Cuts at: 0 1457 1713 2502
Size: 1457 256 789
Fragments arranged by size:
1457 789 256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 889 2502
Size: 889 1613
EcoICRI GAG'CTC
Cuts at: 0 2392 2502
Size: 2392 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 565 575 725 970 1341 1371 1639
Size: 506 59 10 150 245 371 30 268
Cuts at: 1639 1888 2140 2184 2502
Size: 249 252 44 318
Fragments arranged by size:
506 371 318 268 252 249 245 150
59 44 30 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 642 1670 2502
Size: 642 1028 832
Fragments arranged by size:
1028 832 642
EcoRI G'AATT_C
Cuts at: 0 1247 2502
Size: 1247 1255
EcoRV GAT'ATC
Cuts at: 0 1416 2502
Size: 1416 1086
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 220 1012 2502
Size: 220 792 1490
Fragments arranged by size:
1490 792 220
FseI GG_CCGG'CC
Cuts at: 0 1113 2502
Size: 1113 1389
HaeII r_GCGC'y
Cuts at: 0 381 1102 2502
Size: 381 721 1400
Fragments arranged by size:
1400 721 381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 531 563 835 867 1487 1519
Size: 310 32 189 32 272 32 620 32
Cuts at: 1519 1551 1681 1713 1810 1842 2502
Size: 32 130 32 97 32 660
Fragments arranged by size:
660 620 310 272 189 130 97 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 168 273 2502
Size: 168 105 2229
Fragments arranged by size:
2229 168 105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 1025 2502
Size: 314 711 1477
Fragments arranged by size:
1477 711 314
MscI TGG'CCA
Cuts at: 0 1567 2502
Size: 1567 935
MslI CAynn'nnrTG
Cuts at: 0 1256 2467 2502
Size: 1256 1211 35
Fragments arranged by size:
1256 1211 35
MspA1I CmG'CkG
Cuts at: 0 948 2346 2502
Size: 948 1398 156
Fragments arranged by size:
1398 948 156
NaeI GCC'GGC
Cuts at: 0 904 1111 2502
Size: 904 207 1391
Fragments arranged by size:
1391 904 207
NdeI CA'TA_TG
Cuts at: 0 1181 2427 2502
Size: 1181 1246 75
Fragments arranged by size:
1246 1181 75
NgoMIV G'CCGG_C
Cuts at: 0 902 1109 2502
Size: 902 207 1393
Fragments arranged by size:
1393 902 207
NruI TCG'CGA
Cuts at: 0 2295 2502
Size: 2295 207
NspI r_CATG'y
Cuts at: 0 2402 2502
Size: 2402 100
PflMI CCAn_nnn'nTGG
Cuts at: 0 103 296 812 1574 2502
Size: 103 193 516 762 928
Fragments arranged by size:
928 762 516 193 103
PfoI T'CCnGG_A
Cuts at: 0 1865 2502
Size: 1865 637
PstI C_TGCA'G
Cuts at: 0 1322 2276 2502
Size: 1322 954 226
Fragments arranged by size:
1322 954 226
PvuII CAG'CTG
Cuts at: 0 2346 2502
Size: 2346 156
SacI G_AGCT'C
Cuts at: 0 2394 2502
Size: 2394 108
ScaI AGT'ACT
Cuts at: 0 1630 2502
Size: 1630 872
SfcI C'TryA_G
Cuts at: 0 1318 2272 2502
Size: 1318 954 230
Fragments arranged by size:
1318 954 230
SgrAI Cr'CCGG_yG
Cuts at: 0 374 2502
Size: 374 2128
SmaI CCC'GGG
Cuts at: 0 228 2502
Size: 228 2274
SmlI C'TyrA_G
Cuts at: 0 56 642 2480 2502
Size: 56 586 1838 22
Fragments arranged by size:
1838 586 56 22
SspI AAT'ATT
Cuts at: 0 1843 2502
Size: 1843 659
StuI AGG'CCT
Cuts at: 0 1676 2502
Size: 1676 826
StyI C'CwwG_G
Cuts at: 0 451 959 1215 2502
Size: 451 508 256 1287
Fragments arranged by size:
1287 508 451 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 487 2502
Size: 487 2015
TatI w'GTAC_w
Cuts at: 0 717 1176 1628 2502
Size: 717 459 452 874
Fragments arranged by size:
874 717 459 452
Tth111I GACn'n_nGTC
Cuts at: 0 2005 2502
Size: 2005 497
XhoI C'TCGA_G
Cuts at: 0 56 2502
Size: 56 2446
XmaI C'CCGG_G
Cuts at: 0 226 2502
Size: 226 2276
XmnI GAAnn'nnTTC
Cuts at: 0 1247 2053 2078 2502
Size: 1247 806 25 424
Fragments arranged by size:
1247 806 424 25
Enzymes that do cut:
AclI AcuI AflIII AhdI AloI AlwNI ApoI AvaI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI
FseI HaeII Hin4I HincII MmeI MscI MslI MspA1I
NaeI NdeI NgoMIV NruI NspI PflMI PfoI PstI
PvuII SacI ScaI SfcI SgrAI SmaI SmlI SspI
StuI StyI TaqII TatI Tth111I XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AleI
AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI
BsaAI BsaBI BsaXI BsgI BsmBI BspHI BssHII BssSI
BstAPI BstBI BstEII BstZ17I ClaI CspCI EcoO109I FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI NarI
NcoI NheI NotI NsiI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SnaBI SpeI SphI SrfI SwaI
TstI XbaI XcmI ZraI