Restriction map for
pF1KA0440
(Linear) (Six-base) MAPSORT of: KIAA0440.seq Check: 1462 from: 1 to: 5409
KIAA0440.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 5318 5409
Size: 5318 91
AatII G_ACGT'C
Cuts at: 0 4838 5409
Size: 4838 571
AccI GT'mk_AC
Cuts at: 0 1345 1672 5409
Size: 1345 327 3737
Fragments arranged by size:
3737 1345 327
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 1892 2615 4224 4827 5260 5275 5386
Size: 488 1404 723 1609 603 433 15 111
Cuts at: 5386 5409
Size: 23
Fragments arranged by size:
1609 1404 723 603 488 433 111 23
15
AflIII A'CryG_T
Cuts at: 0 976 1449 5409
Size: 976 473 3960
Fragments arranged by size:
3960 976 473
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 433 465 1928 1960 5409
Size: 433 32 1463 32 3449
Fragments arranged by size:
3449 1463 433 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 971 1543 1755 2100 2246 5160 5310 5409
Size: 971 572 212 345 146 2914 150 99
Fragments arranged by size:
2914 971 572 345 212 150 146 99
ApaLI G'TGCA_C
Cuts at: 0 2221 5409
Size: 2221 3188
ApoI r'AATT_y
Cuts at: 0 1176 1302 1480 1918 3169 5409
Size: 1176 126 178 438 1251 2240
Fragments arranged by size:
2240 1251 1176 438 178 126
AseI AT'TA_AT
Cuts at: 0 2366 3778 5409
Size: 2366 1412 1631
Fragments arranged by size:
2366 1631 1412
AvaI C'yCGr_G
Cuts at: 0 486 3688 3843 5409
Size: 486 3202 155 1566
Fragments arranged by size:
3202 1566 486 155
AvrII C'CTAG_G
Cuts at: 0 4408 5409
Size: 4408 1001
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4254 4287 5409
Size: 4254 33 1122
Fragments arranged by size:
4254 1122 33
BamHI G'GATC_C
Cuts at: 0 3789 5409
Size: 3789 1620
BanI G'GyrC_C
Cuts at: 0 73 3356 3961 4077 5409
Size: 73 3283 605 116 1332
Fragments arranged by size:
3283 1332 605 116 73
BanII G_rGCy'C
Cuts at: 0 1631 1742 2558 2936 3766 4282 5409
Size: 1631 111 816 378 830 516 1127
Fragments arranged by size:
1631 1127 830 816 516 378 111
BbeI G_GCGC'C
Cuts at: 0 3360 5409
Size: 3360 2049
BbsI GAAGACnn'nnnn_
Cuts at: 0 962 2603 2679 3452 4160 5282 5379 5409
Size: 962 1641 76 773 708 1122 97 30
Fragments arranged by size:
1641 1122 962 773 708 97 76 30
BbvCI CC'TCA_GC
Cuts at: 0 1192 2962 5409
Size: 1192 1770 2447
Fragments arranged by size:
2447 1770 1192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2956 2990 5409
Size: 2956 34 2419
Fragments arranged by size:
2956 2419 34
BclI T'GATC_A
Cuts at: 0 5139 5409
Size: 5139 270
BglI GCCn_nnn'nGGC
Cuts at: 0 2953 3609 4205 5409
Size: 2953 656 596 1204
Fragments arranged by size:
2953 1204 656 596
BglII A'GATC_T
Cuts at: 0 933 1455 2442 2987 4617 5409
Size: 933 522 987 545 1630 792
Fragments arranged by size:
1630 987 933 792 545 522
BlpI GC'TnA_GC
Cuts at: 0 2557 5409
Size: 2557 2852
Bme1580I G_kGCm'C
Cuts at: 0 1397 2225 3493 3891 4320 5409
Size: 1397 828 1268 398 429 1089
Fragments arranged by size:
1397 1268 1089 828 429 398
BmrI ACTGGGnnnn_n'
Cuts at: 0 3426 3564 3761 4294 5409
Size: 3426 138 197 533 1115
Fragments arranged by size:
3426 1115 533 197 138
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 512 829 1966 2572 5409
Size: 512 317 1137 606 2837
Fragments arranged by size:
2837 1137 606 512 317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 674 890 1103 1675 1741 2174 3296
Size: 435 239 216 213 572 66 433 1122
Cuts at: 3296 3330 3837 3855 4080 4782 5167 5409
Size: 34 507 18 225 702 385 242
Fragments arranged by size:
1122 702 572 507 435 433 385 242
239 225 216 213 66 34 18
Bpu10I CC'TnA_GC
Cuts at: 0 135 1192 1736 2962 4053 4174 4586 5409
Size: 135 1057 544 1226 1091 121 412 823
Fragments arranged by size:
1226 1091 1057 823 544 412 135 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 829 3221 5139 5409
Size: 291 538 2392 1918 270
Fragments arranged by size:
2392 1918 538 291 270
BsaI GGTCTCn'nnnn_
Cuts at: 0 1119 3411 4562 4960 5409
Size: 1119 2292 1151 398 449
Fragments arranged by size:
2292 1151 1119 449 398
BsaAI yAC'GTr
Cuts at: 0 1452 1728 4853 5409
Size: 1452 276 3125 556
Fragments arranged by size:
3125 1452 556 276
BsaBI GATnn'nnATC
Cuts at: 0 1526 5409
Size: 1526 3883
BsaHI Gr'CG_yC
Cuts at: 0 1661 3357 4804 4835 5409
Size: 1661 1696 1447 31 574
Fragments arranged by size:
1696 1661 1447 574 31
BsaWI w'CCGG_w
Cuts at: 0 116 4154 5409
Size: 116 4038 1255
Fragments arranged by size:
4038 1255 116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 433 463 466 496 1367 1397 1928 1958
Size: 433 30 3 30 871 30 531 30
Cuts at: 1958 2081 2111 2753 2783 4672 4702 5409
Size: 123 30 642 30 1889 30 707
Fragments arranged by size:
1889 871 707 642 531 433 123 30
30 30 30 30 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 867 1080 2774 2812 3117 3299 4371 4374
Size: 867 213 1694 38 305 182 1072 3
Cuts at: 4374 4383 4386 4389 4680 4685 4899 5184 5353
Size: 9 3 3 291 5 214 285 169
Cuts at: 5353 5409
Size: 56
Fragments arranged by size:
1694 1072 867 305 291 285 214 213
182 169 56 38 9 5 3 3
3
BseYI C'CCAG_C
Cuts at: 0 478 1905 3543 3726 4003 4026 4711 5409
Size: 478 1427 1638 183 277 23 685 698
Fragments arranged by size:
1638 1427 698 685 478 277 183 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 3075 3274 5323 5409
Size: 589 2486 199 2049 86
Fragments arranged by size:
2486 2049 589 199 86
BsiHKAI G_wGCw'C
Cuts at: 0 1631 1727 1742 1870 1977 2160 2225 2236
Size: 1631 96 15 128 107 183 65 11
Cuts at: 2236 2558 2660 4282 4787 5409
Size: 322 102 1622 505 622
Fragments arranged by size:
1631 1622 622 505 322 183 128 107
102 96 65 15 11
BsmI GAATG_Cn'
Cuts at: 0 1550 3285 3825 5409
Size: 1550 1735 540 1584
Fragments arranged by size:
1735 1584 1550 540
Bsp1286I G_dGCh'C
Cuts at: 0 1397 1631 1727 1742 1870 1977 2160 2225
Size: 1397 234 96 15 128 107 183 65
Cuts at: 2225 2236 2558 2660 2936 3493 3766 3891 4282
Size: 11 322 102 276 557 273 125 391
Cuts at: 4282 4320 4787 5409
Size: 38 467 622
Fragments arranged by size:
1397 622 557 467 391 322 276 273
234 183 128 125 107 102 96 65
38 15 11
BspHI T'CATG_A
Cuts at: 0 1631 1796 5409
Size: 1631 165 3613
Fragments arranged by size:
3613 1631 165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 5318 5409
Size: 5318 91
BsrDI GCAATG_nn'
Cuts at: 0 906 1227 1359 3629 3882 4536 5409
Size: 906 321 132 2270 253 654 873
Fragments arranged by size:
2270 906 873 654 321 253 132
BsrFI r'CCGG_y
Cuts at: 0 4204 5409
Size: 4204 1205
BsrGI T'GTAC_A
Cuts at: 0 1883 2018 5409
Size: 1883 135 3391
Fragments arranged by size:
3391 1883 135
BssSI C'ACGA_G
Cuts at: 0 4503 5409
Size: 4503 906
BstAPI GCAn_nnn'nTGC
Cuts at: 0 238 2377 4983 5310 5409
Size: 238 2139 2606 327 99
Fragments arranged by size:
2606 2139 327 238 99
BstBI TT'CG_AA
Cuts at: 0 1554 5409
Size: 1554 3855
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2152 4302 5409
Size: 2152 2150 1107
Fragments arranged by size:
2152 2150 1107
BstYI r'GATC_y
Cuts at: 0 933 1455 1588 2442 2636 2987 3427 3538
Size: 933 522 133 854 194 351 440 111
Cuts at: 3538 3789 4617 5409
Size: 251 828 792
Fragments arranged by size:
933 854 828 792 522 440 351 251
194 133 111
Bsu36I CC'TnA_GG
Cuts at: 0 814 2101 3665 4902 5326 5409
Size: 814 1287 1564 1237 424 83
Fragments arranged by size:
1564 1287 1237 814 424 83
BtgI C'CryG_G
Cuts at: 0 1100 2408 2612 3097 3413 4008 4112 4143
Size: 1100 1308 204 485 316 595 104 31
Cuts at: 4143 4487 5409
Size: 344 922
Fragments arranged by size:
1308 1100 922 595 485 344 316 204
104 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4538 5095 5409
Size: 4538 557 314
Fragments arranged by size:
4538 557 314
BtsI GCAGTG_nn'
Cuts at: 0 601 5015 5409
Size: 601 4414 394
Fragments arranged by size:
4414 601 394
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2663 2698 5409
Size: 2663 35 2711
Fragments arranged by size:
2711 2663 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 432 5409
Size: 432 4977
EarI CTCTTCn'nnn_
Cuts at: 0 503 1871 4331 4392 4660 4846 5409
Size: 503 1368 2460 61 268 186 563
Fragments arranged by size:
2460 1368 563 503 268 186 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2938 5311 5409
Size: 2938 2373 98
Fragments arranged by size:
2938 2373 98
EcoICRI GAG'CTC
Cuts at: 0 1629 1740 2556 4280 5409
Size: 1629 111 816 1724 1129
Fragments arranged by size:
1724 1629 1129 816 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 488 674 890 1103 1675 1741 1892
Size: 435 53 186 216 213 572 66 151
Cuts at: 1892 2174 2615 3296 3330 3837 3855 4080 4224
Size: 282 441 681 34 507 18 225 144
Cuts at: 4224 4782 4827 5167 5260 5275 5386 5409
Size: 558 45 340 93 15 111 23
Fragments arranged by size:
681 572 558 507 441 435 340 282
225 216 213 186 151 144 111 93
66 53 45 34 23 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4052 5314 5409
Size: 4052 1262 95
Fragments arranged by size:
4052 1262 95
EcoO109I rG'GnC_Cy
Cuts at: 0 50 163 968 1170 3223 3661 5409
Size: 50 113 805 202 2053 438 1748
Fragments arranged by size:
2053 1748 805 438 202 113 50
EcoRI G'AATT_C
Cuts at: 0 1176 1918 3169 5409
Size: 1176 742 1251 2240
Fragments arranged by size:
2240 1251 1176 742
EcoRV GAT'ATC
Cuts at: 0 531 2127 5409
Size: 531 1596 3282
Fragments arranged by size:
3282 1596 531
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1422 1939 3198 4357 5305 5409
Size: 1422 517 1259 1159 948 104
Fragments arranged by size:
1422 1259 1159 948 517 104
FspI TGC'GCA
Cuts at: 0 898 5409
Size: 898 4511
HaeII r_GCGC'y
Cuts at: 0 114 3360 4436 5409
Size: 114 3246 1076 973
Fragments arranged by size:
3246 1076 973 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 310 342 682 714 934 966
Size: 37 32 241 32 340 32 220 32
Cuts at: 966 1634 1666 3294 3326 3976 4008 4497 4529
Size: 668 32 1628 32 650 32 489 32
Cuts at: 4529 5409
Size: 880
Fragments arranged by size:
1628 880 668 650 489 340 241 220
37 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1673 2904 5409
Size: 1673 1231 2505
Fragments arranged by size:
2505 1673 1231
HindIII A'AGCT_T
Cuts at: 0 660 5409
Size: 660 4749
KasI G'GCGC_C
Cuts at: 0 3356 5409
Size: 3356 2053
MfeI C'AATT_G
Cuts at: 0 632 2135 2691 5409
Size: 632 1503 556 2718
Fragments arranged by size:
2718 1503 632 556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 736 742 3418 4733 5063 5156 5409
Size: 416 320 6 2676 1315 330 93 253
Fragments arranged by size:
2676 1315 416 330 320 253 93 6
MslI CAynn'nnrTG
Cuts at: 0 92 1001 3130 3397 3896 4083 5401 5409
Size: 92 909 2129 267 499 187 1318 8
Fragments arranged by size:
2129 1318 909 499 267 187 92 8
MspA1I CmG'CkG
Cuts at: 0 1096 1900 3099 3590 4351 5145 5226 5409
Size: 1096 804 1199 491 761 794 81 183
Fragments arranged by size:
1199 1096 804 794 761 491 183 81
NaeI GCC'GGC
Cuts at: 0 4206 5409
Size: 4206 1203
NarI GG'CG_CC
Cuts at: 0 3357 5409
Size: 3357 2052
NcoI C'CATG_G
Cuts at: 0 2408 2612 3413 4112 5409
Size: 2408 204 801 699 1297
Fragments arranged by size:
2408 1297 801 699 204
NgoMIV G'CCGG_C
Cuts at: 0 4204 5409
Size: 4204 1205
NspI r_CATG'y
Cuts at: 0 108 980 3129 3719 5409
Size: 108 872 2149 590 1690
Fragments arranged by size:
2149 1690 872 590 108
PciI A'CATG_T
Cuts at: 0 976 5409
Size: 976 4433
PflMI CCAn_nnn'nTGG
Cuts at: 0 2579 4149 5409
Size: 2579 1570 1260
Fragments arranged by size:
2579 1570 1260
PfoI T'CCnGG_A
Cuts at: 0 1652 2544 4961 5409
Size: 1652 892 2417 448
Fragments arranged by size:
2417 1652 892 448
PmlI CAC'GTG
Cuts at: 0 1728 5409
Size: 1728 3681
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 433 465 1928 1960 1998 2030 5409
Size: 433 32 1463 32 38 32 3379
Fragments arranged by size:
3379 1463 433 38 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 163 1170 3661 5409
Size: 163 1007 2491 1748
Fragments arranged by size:
2491 1748 1007 163
PshAI GACnn'nnGTC
Cuts at: 0 1125 5409
Size: 1125 4284
PspXI vC'TCGA_Gb
Cuts at: 0 3688 3843 5409
Size: 3688 155 1566
Fragments arranged by size:
3688 1566 155
PstI C_TGCA'G
Cuts at: 0 5025 5409
Size: 5025 384
PvuII CAG'CTG
Cuts at: 0 1096 1900 4351 5145 5226 5409
Size: 1096 804 2451 794 81 183
Fragments arranged by size:
2451 1096 804 794 183 81
SacI G_AGCT'C
Cuts at: 0 1631 1742 2558 4282 5409
Size: 1631 111 816 1724 1127
Fragments arranged by size:
1724 1631 1127 816 111
SacII CC_GC'GG
Cuts at: 0 3100 5409
Size: 3100 2309
SalI G'TCGA_C
Cuts at: 0 1671 5409
Size: 1671 3738
SapI GCTCTTCn'nnn_
Cuts at: 0 503 4331 5409
Size: 503 3828 1078
Fragments arranged by size:
3828 1078 503
ScaI AGT'ACT
Cuts at: 0 1385 5409
Size: 1385 4024
SexAI A'CCwGG_T
Cuts at: 0 2266 5042 5409
Size: 2266 2776 367
Fragments arranged by size:
2776 2266 367
SfcI C'TryA_G
Cuts at: 0 158 2700 5021 5332 5409
Size: 158 2542 2321 311 77
Fragments arranged by size:
2542 2321 311 158 77
SfoI GGC'GCC
Cuts at: 0 3358 5409
Size: 3358 2051
SmlI C'TyrA_G
Cuts at: 0 306 844 3236 3688 3843 5118 5409
Size: 306 538 2392 452 155 1275 291
Fragments arranged by size:
2392 1275 538 452 306 291 155
SpeI A'CTAG_T
Cuts at: 0 1621 5409
Size: 1621 3788
SphI G_CATG'C
Cuts at: 0 3129 5409
Size: 3129 2280
StuI AGG'CCT
Cuts at: 0 33 1700 4890 5409
Size: 33 1667 3190 519
Fragments arranged by size:
3190 1667 519 33
StyI C'CwwG_G
Cuts at: 0 1109 1397 1778 2408 2612 3413 4112 4408
Size: 1109 288 381 630 204 801 699 296
Cuts at: 4408 5409
Size: 1001
Fragments arranged by size:
1109 1001 801 699 630 381 296 288
204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1450 1638 1791 2094 3583 5409
Size: 1450 188 153 303 1489 1826
Fragments arranged by size:
1826 1489 1450 303 188 153
TatI w'GTAC_w
Cuts at: 0 1383 1847 1883 2018 3140 5409
Size: 1383 464 36 135 1122 2269
Fragments arranged by size:
2269 1383 1122 464 135 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3224 3256 4672 4704 5409
Size: 3224 32 1416 32 705
Fragments arranged by size:
3224 1416 705 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 961 2242 5409
Size: 961 1281 3167
Fragments arranged by size:
3167 1281 961
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1404 3577 4485 5409
Size: 1404 2173 908 924
Fragments arranged by size:
2173 1404 924 908
XhoI C'TCGA_G
Cuts at: 0 3688 3843 5409
Size: 3688 155 1566
Fragments arranged by size:
3688 1566 155
XmnI GAAnn'nnTTC
Cuts at: 0 1922 2010 4340 5371 5409
Size: 1922 88 2330 1031 38
Fragments arranged by size:
2330 1922 1031 88 38
ZraI GAC'GTC
Cuts at: 0 4836 5409
Size: 4836 573
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AloI AlwNI ApaLI
ApoI AseI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DrdI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI MfeI MmeI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmlI PpiI PpuMI
PshAI PspXI PstI PvuII SacI SacII SalI SapI
ScaI SexAI SfcI SfoI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI
ApaI AscI AsiSI BciVI BfrBI BmgBI BmtI BsiEI
BsiWI BsmBI BspEI BsrBI BssHII BstEII BstZ17I ClaI
DraI DraIII EaeI EagI FseI FspAI HpaI KpnI
MluI MscI NdeI NheI NotI NruI NsiI PacI
PasI PmeI PsiI PspOMI PsrI PvuI RsrII SanDI
SbfI SfiI SgfI SgrAI SmaI SnaBI SrfI SspI
SwaI XbaI XmaI