Restriction map for
pF1KA0447
(Linear) (Six-base) MAPSORT of: KIAA0447.seq Check: 9241 from: 1 to: 1215
KIAA0447.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AatII G_ACGT'C
Cuts at: 0 839 1215
Size: 839 376
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 852 910 1215
Size: 237 615 58 305
Fragments arranged by size:
615 305 237 58
AleI CACnn'nnGTG
Cuts at: 0 107 1215
Size: 107 1108
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 760 792 1215
Size: 760 32 423
Fragments arranged by size:
760 423 32
AlwNI CAG_nnn'CTG
Cuts at: 0 260 336 1140 1153 1215
Size: 260 76 804 13 62
Fragments arranged by size:
804 260 76 62 13
ApaI G_GGCC'C
Cuts at: 0 1164 1215
Size: 1164 51
ApaLI G'TGCA_C
Cuts at: 0 636 1215
Size: 636 579
AvaI C'yCGr_G
Cuts at: 0 818 1215
Size: 818 397
BanII G_rGCy'C
Cuts at: 0 91 217 311 532 1164 1215
Size: 91 126 94 221 632 51
Fragments arranged by size:
632 221 126 94 91 51
BclI T'GATC_A
Cuts at: 0 551 1215
Size: 551 664
BlpI GC'TnA_GC
Cuts at: 0 736 1018 1215
Size: 736 282 197
Fragments arranged by size:
736 282 197
Bme1580I G_kGCm'C
Cuts at: 0 640 1164 1215
Size: 640 524 51
Fragments arranged by size:
640 524 51
BmgBI CAC'GTC
Cuts at: 0 885 1215
Size: 885 330
BmrI ACTGGGnnnn_n'
Cuts at: 0 607 835 1215
Size: 607 228 380
Fragments arranged by size:
607 380 228
BsaI GGTCTCn'nnnn_
Cuts at: 0 172 1215
Size: 172 1043
BsaHI Gr'CG_yC
Cuts at: 0 836 1215
Size: 836 379
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 760 790 1215
Size: 760 30 425
Fragments arranged by size:
760 425 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 867 1215
Size: 867 348
BseYI C'CCAG_C
Cuts at: 0 21 83 297 310 1215
Size: 21 62 214 13 905
Fragments arranged by size:
905 214 62 21 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 349 1215
Size: 246 103 866
Fragments arranged by size:
866 246 103
BsiEI CG_ry'CG
Cuts at: 0 680 1061 1215
Size: 680 381 154
Fragments arranged by size:
680 381 154
BsiHKAI G_wGCw'C
Cuts at: 0 217 532 640 1215
Size: 217 315 108 575
Fragments arranged by size:
575 315 217 108
BsiWI C'GTAC_G
Cuts at: 0 941 1215
Size: 941 274
BsmBI CGTCTCn'nnnn_
Cuts at: 0 511 1215
Size: 511 704
Bsp1286I G_dGCh'C
Cuts at: 0 91 217 311 532 640 1164 1215
Size: 91 126 94 221 108 524 51
Fragments arranged by size:
524 221 126 108 94 91 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 59 1215
Size: 59 1156
BsrBI CCG'CTC
Cuts at: 0 857 1215
Size: 857 358
BstEII G'GTnAC_C
Cuts at: 0 100 1077 1215
Size: 100 977 138
Fragments arranged by size:
977 138 100
BstXI CCAn_nnnn'nTGG
Cuts at: 0 699 1215
Size: 699 516
BtgI C'CryG_G
Cuts at: 0 182 344 1215
Size: 182 162 871
Fragments arranged by size:
871 182 162
DraIII CAC_nnn'GTG
Cuts at: 0 242 1215
Size: 242 973
EaeI y'GGCC_r
Cuts at: 0 641 677 800 1058 1155 1215
Size: 641 36 123 258 97 60
Fragments arranged by size:
641 258 123 97 60 36
EagI C'GGCC_G
Cuts at: 0 677 1058 1215
Size: 677 381 157
Fragments arranged by size:
677 381 157
EarI CTCTTCn'nnn_
Cuts at: 0 26 47 274 520 856 1215
Size: 26 21 227 246 336 359
Fragments arranged by size:
359 336 246 227 26 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 522 1215
Size: 522 693
EcoICRI GAG'CTC
Cuts at: 0 215 530 1215
Size: 215 315 685
Fragments arranged by size:
685 315 215
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 852 910 1215
Size: 237 615 58 305
Fragments arranged by size:
615 305 237 58
EcoO109I rG'GnC_Cy
Cuts at: 0 1089 1161 1197 1215
Size: 1089 72 36 18
Fragments arranged by size:
1089 72 36 18
HaeII r_GCGC'y
Cuts at: 0 226 634 791 1132 1215
Size: 226 408 157 341 83
Fragments arranged by size:
408 341 226 157 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 543 575 1215
Size: 543 32 640
Fragments arranged by size:
640 543 32
HincII GTy'rAC
Cuts at: 0 597 1215
Size: 597 618
MscI TGG'CCA
Cuts at: 0 802 1215
Size: 802 413
MslI CAynn'nnrTG
Cuts at: 0 107 1215
Size: 107 1108
MspA1I CmG'CkG
Cuts at: 0 83 740 793 1185 1215
Size: 83 657 53 392 30
Fragments arranged by size:
657 392 83 53 30
NspI r_CATG'y
Cuts at: 0 318 1003 1215
Size: 318 685 212
Fragments arranged by size:
685 318 212
PflMI CCAn_nnn'nTGG
Cuts at: 0 885 1215
Size: 885 330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 760 792 1215
Size: 760 32 423
Fragments arranged by size:
760 423 32
PpuMI rG'GwC_Cy
Cuts at: 0 1089 1197 1215
Size: 1089 108 18
Fragments arranged by size:
1089 108 18
PshAI GACnn'nnGTC
Cuts at: 0 184 1215
Size: 184 1031
PspOMI G'GGCC_C
Cuts at: 0 1160 1215
Size: 1160 55
PstI C_TGCA'G
Cuts at: 0 776 1136 1149 1215
Size: 776 360 13 66
Fragments arranged by size:
776 360 66 13
PvuII CAG'CTG
Cuts at: 0 83 1215
Size: 83 1132
SacI G_AGCT'C
Cuts at: 0 217 532 1215
Size: 217 315 683
Fragments arranged by size:
683 315 217
SapI GCTCTTCn'nnn_
Cuts at: 0 26 274 520 856 1215
Size: 26 248 246 336 359
Fragments arranged by size:
359 336 248 246 26
SfcI C'TryA_G
Cuts at: 0 772 1132 1145 1215
Size: 772 360 13 70
Fragments arranged by size:
772 360 70 13
SmaI CCC'GGG
Cuts at: 0 820 1215
Size: 820 395
SphI G_CATG'C
Cuts at: 0 318 1215
Size: 318 897
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 193 432 1215
Size: 193 239 783
Fragments arranged by size:
783 239 193
TatI w'GTAC_w
Cuts at: 0 281 578 1215
Size: 281 297 637
Fragments arranged by size:
637 297 281
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 407 439 1215
Size: 407 32 776
Fragments arranged by size:
776 407 32
Tth111I GACn'n_nGTC
Cuts at: 0 910 1215
Size: 910 305
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 696 1215
Size: 696 519
XmaI C'CCGG_G
Cuts at: 0 818 1215
Size: 818 397
XmnI GAAnn'nnTTC
Cuts at: 0 865 1215
Size: 865 350
ZraI GAC'GTC
Cuts at: 0 837 1215
Size: 837 378
Enzymes that do cut:
AatII AcuI AleI AloI AlwNI ApaI ApaLI AvaI
BanII BclI BlpI Bme1580I BmgBI BmrI BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspMI BsrBI BstEII BstXI BtgI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I
HincII MscI MslI MspA1I NspI PflMI PpiI PpuMI
PshAI PspOMI PstI PvuII SacI SapI SfcI SmaI
SphI TaqII TatI TstI Tth111I XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BglII BmtI BplI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaWI BsmI BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DrdI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI StuI StyI
SwaI XbaI XhoI