Restriction map for
pF1KA0449
(Linear) (Six-base) MAPSORT of: KIAA0449.seq Check: 6487 from: 1 to: 4872
KIAA0449.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AatII G_ACGT'C
Cuts at: 0 3643 4872
Size: 3643 1229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 859 2254 2706 3104 3272 4872
Size: 631 228 1395 452 398 168 1600
Fragments arranged by size:
1600 1395 631 452 398 228 168
AfeI AGC'GCT
Cuts at: 0 3794 4872
Size: 3794 1078
AflII C'TTAA_G
Cuts at: 0 2203 4872
Size: 2203 2669
AleI CACnn'nnGTG
Cuts at: 0 199 3007 3055 3697 3855 4872
Size: 199 2808 48 642 158 1017
Fragments arranged by size:
2808 1017 642 199 158 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1172 4872
Size: 1172 3700
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2833 2865 4872
Size: 2833 32 2007
Fragments arranged by size:
2833 2007 32
AlwNI CAG_nnn'CTG
Cuts at: 0 120 441 609 1641 1944 2169 2754 2931
Size: 120 321 168 1032 303 225 585 177
Cuts at: 2931 3071 4649 4778 4872
Size: 140 1578 129 94
Fragments arranged by size:
1578 1032 585 321 303 225 177 168
140 129 120 94
ApaI G_GGCC'C
Cuts at: 0 624 1101 1299 2589 3463 4872
Size: 624 477 198 1290 874 1409
Fragments arranged by size:
1409 1290 874 624 477 198
ApaLI G'TGCA_C
Cuts at: 0 714 952 1157 3836 4872
Size: 714 238 205 2679 1036
Fragments arranged by size:
2679 1036 714 238 205
AvaI C'yCGr_G
Cuts at: 0 102 339 774 1687 1833 1873 2905 3262
Size: 102 237 435 913 146 40 1032 357
Cuts at: 3262 3599 3626 4038 4125 4251 4523 4623 4872
Size: 337 27 412 87 126 272 100 249
Fragments arranged by size:
1032 913 435 412 357 337 272 249
237 146 126 102 100 87 40 27
AvrII C'CTAG_G
Cuts at: 0 3616 4872
Size: 3616 1256
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 72 105 4872
Size: 72 33 4767
Fragments arranged by size:
4767 72 33
BamHI G'GATC_C
Cuts at: 0 41 4872
Size: 41 4831
BanI G'GyrC_C
Cuts at: 0 2468 2671 4231 4432 4872
Size: 2468 203 1560 201 440
Fragments arranged by size:
2468 1560 440 203 201
BanII G_rGCy'C
Cuts at: 0 113 449 624 667 1045 1101 1299 1540
Size: 113 336 175 43 378 56 198 241
Cuts at: 1540 1687 2400 2589 2905 3416 3463 4463 4568
Size: 147 713 189 316 511 47 1000 105
Cuts at: 4568 4872
Size: 304
Fragments arranged by size:
1000 713 511 378 336 316 304 241
198 189 175 147 113 105 56 47
43
BbeI G_GCGC'C
Cuts at: 0 2472 4872
Size: 2472 2400
BbsI GAAGACnn'nnnn_
Cuts at: 0 150 283 2449 3747 4872
Size: 150 133 2166 1298 1125
Fragments arranged by size:
2166 1298 1125 150 133
BbvCI CC'TCA_GC
Cuts at: 0 1241 2074 2543 2936 4219 4872
Size: 1241 833 469 393 1283 653
Fragments arranged by size:
1283 1241 833 653 469 393
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 195 229 4872
Size: 195 34 4643
Fragments arranged by size:
4643 195 34
BclI T'GATC_A
Cuts at: 0 917 4872
Size: 917 3955
BglI GCCn_nnn'nGGC
Cuts at: 0 1096 1570 2254 2278 4229 4319 4872
Size: 1096 474 684 24 1951 90 553
Fragments arranged by size:
1951 1096 684 553 474 90 24
BglII A'GATC_T
Cuts at: 0 185 3326 4872
Size: 185 3141 1546
Fragments arranged by size:
3141 1546 185
BlpI GC'TnA_GC
Cuts at: 0 4279 4642 4872
Size: 4279 363 230
Fragments arranged by size:
4279 363 230
Bme1580I G_kGCm'C
Cuts at: 0 295 393 624 718 956 1101 1161 1299
Size: 295 98 231 94 238 145 60 138
Cuts at: 1299 2589 3463 3840 4234 4435 4872
Size: 1290 874 377 394 201 437
Fragments arranged by size:
1290 874 437 394 377 295 238 231
201 145 138 98 94 60
BmgBI CAC'GTC
Cuts at: 0 4121 4872
Size: 4121 751
BmrI ACTGGGnnnn_n'
Cuts at: 0 429 758 3351 4608 4872
Size: 429 329 2593 1257 264
Fragments arranged by size:
2593 1257 429 329 264
BmtI G_CTAG'C
Cuts at: 0 1393 4872
Size: 1393 3479
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 897 1626 3057 3345 4872
Size: 897 729 1431 288 1527
Fragments arranged by size:
1527 1431 897 729 288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 976 1544 1663 1978 2068 2422 2583
Size: 463 513 568 119 315 90 354 161
Cuts at: 2583 2776 2827 3037 4755 4872
Size: 193 51 210 1718 117
Fragments arranged by size:
1718 568 513 463 354 315 210 193
161 119 117 90 51
Bpu10I CC'TnA_GC
Cuts at: 0 1241 2074 2543 2936 4187 4219 4872
Size: 1241 833 469 393 1251 32 653
Fragments arranged by size:
1251 1241 833 653 469 393 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1972 2344 2725 3097 4872
Size: 1972 372 381 372 1775
Fragments arranged by size:
1972 1775 381 372 372
BsaI GGTCTCn'nnnn_
Cuts at: 0 1060 2323 3016 3545 3579 4020 4872
Size: 1060 1263 693 529 34 441 852
Fragments arranged by size:
1263 1060 852 693 529 441 34
BsaBI GATnn'nnATC
Cuts at: 0 1930 4872
Size: 1930 2942
BsaHI Gr'CG_yC
Cuts at: 0 563 2469 3233 3640 4872
Size: 563 1906 764 407 1232
Fragments arranged by size:
1906 1232 764 563 407
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 955 985 2833 2863 4000 4030 4872
Size: 955 30 1848 30 1137 30 842
Fragments arranged by size:
1848 1137 955 842 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 334 337 472 607 979 1471 1753 1777
Size: 334 3 135 135 372 492 282 24
Cuts at: 1777 1780 1783 1813 1816 1891 2113 2317 2451
Size: 3 3 30 3 75 222 204 134
Cuts at: 2451 2857 2953 3034 3046 3198 3337 3839 4872
Size: 406 96 81 12 152 139 502 1033
Fragments arranged by size:
1033 502 492 406 372 334 282 222
204 152 139 135 135 134 96 81
75 30 24 12 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 50 123 719 1002 1358 4413 4872
Size: 50 73 596 283 356 3055 459
Fragments arranged by size:
3055 596 459 356 283 73 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 2444 2528 3031 3310 4725 4872
Size: 679 1765 84 503 279 1415 147
Fragments arranged by size:
1765 1415 679 503 279 147 84
BsiEI CG_ry'CG
Cuts at: 0 2058 3672 4872
Size: 2058 1614 1200
Fragments arranged by size:
2058 1614 1200
BsiHKAI G_wGCw'C
Cuts at: 0 449 667 718 956 1161 2192 2400 3840
Size: 449 218 51 238 205 1031 208 1440
Cuts at: 3840 4188 4568 4872
Size: 348 380 304
Fragments arranged by size:
1440 1031 449 380 348 304 238 218
208 205 51
BsmI GAATG_Cn'
Cuts at: 0 1275 4106 4872
Size: 1275 2831 766
Fragments arranged by size:
2831 1275 766
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1735 1775 3631 3769 4872
Size: 1735 40 1856 138 1103
Fragments arranged by size:
1856 1735 1103 138 40
Bsp1286I G_dGCh'C
Cuts at: 0 113 295 393 449 624 667 718 956
Size: 113 182 98 56 175 43 51 238
Cuts at: 956 1045 1101 1161 1299 1540 1687 2192 2400
Size: 89 56 60 138 241 147 505 208
Cuts at: 2400 2589 2905 3416 3463 3840 4188 4234 4435
Size: 189 316 511 47 377 348 46 201
Cuts at: 4435 4463 4568 4872
Size: 28 105 304
Fragments arranged by size:
511 505 377 348 316 304 241 238
208 201 189 182 175 147 138 113
105 98 89 60 56 56 51 47
46 43 28
BspHI T'CATG_A
Cuts at: 0 1025 1364 4872
Size: 1025 339 3508
Fragments arranged by size:
3508 1025 339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 500 1391 2168 4274 4872
Size: 500 891 777 2106 598
Fragments arranged by size:
2106 891 777 598 500
BsrBI CCG'CTC
Cuts at: 0 834 864 1647 2493 2591 2823 2886 4872
Size: 834 30 783 846 98 232 63 1986
Fragments arranged by size:
1986 846 834 783 232 98 63 30
BsrDI GCAATG_nn'
Cuts at: 0 368 918 1416 3747 3756 4263 4872
Size: 368 550 498 2331 9 507 609
Fragments arranged by size:
2331 609 550 507 498 368 9
BsrFI r'CCGG_y
Cuts at: 0 4 823 1399 4872
Size: 4 819 576 3473
Fragments arranged by size:
3473 819 576 4
BssSI C'ACGA_G
Cuts at: 0 529 4369 4872
Size: 529 3840 503
Fragments arranged by size:
3840 529 503
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2169 2506 4872
Size: 2169 337 2366
Fragments arranged by size:
2366 2169 337
BstEII G'GTnAC_C
Cuts at: 0 4341 4872
Size: 4341 531
BstXI CCAn_nnnn'nTGG
Cuts at: 0 249 1599 3501 3699 4011 4872
Size: 249 1350 1902 198 312 861
Fragments arranged by size:
1902 1350 861 312 249 198
BstYI r'GATC_y
Cuts at: 0 41 185 461 524 2030 2390 3161 3326
Size: 41 144 276 63 1506 360 771 165
Cuts at: 3326 4872
Size: 1546
Fragments arranged by size:
1546 1506 771 360 276 165 144 63
41
Bsu36I CC'TnA_GG
Cuts at: 0 927 2434 4872
Size: 927 1507 2438
Fragments arranged by size:
2438 1507 927
BtgI C'CryG_G
Cuts at: 0 242 287 1598 2081 3056 3866 4872
Size: 242 45 1311 483 975 810 1006
Fragments arranged by size:
1311 1006 975 810 483 242 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 333 1418 4872
Size: 333 1085 3454
Fragments arranged by size:
3454 1085 333
BtsI GCAGTG_nn'
Cuts at: 0 794 1159 1845 2866 3850 3862 4872
Size: 794 365 686 1021 984 12 1010
Fragments arranged by size:
1021 1010 984 794 686 365 12
ClaI AT'CG_AT
Cuts at: 0 1334 4872
Size: 1334 3538
DraIII CAC_nnn'GTG
Cuts at: 0 813 4872
Size: 813 4059
DrdI GACnn_nn'nnGTC
Cuts at: 0 22 3751 4119 4872
Size: 22 3729 368 753
Fragments arranged by size:
3729 753 368 22
EaeI y'GGCC_r
Cuts at: 0 2 6 32 821 1907 2474 2552 3869
Size: 2 4 26 789 1086 567 78 1317
Cuts at: 3869 4311 4352 4841 4872
Size: 442 41 489 31
Fragments arranged by size:
1317 1086 789 567 489 442 78 41
31 26 4 2
EarI CTCTTCn'nnn_
Cuts at: 0 1675 1769 1838 2128 2906 4872
Size: 1675 94 69 290 778 1966
Fragments arranged by size:
1966 1675 778 290 94 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1774 1915 4872
Size: 1774 141 2957
Fragments arranged by size:
2957 1774 141
EcoICRI GAG'CTC
Cuts at: 0 447 665 2398 4566 4872
Size: 447 218 1733 2168 306
Fragments arranged by size:
2168 1733 447 306 218
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 631 859 976 1544 1663 1978 2068
Size: 463 168 228 117 568 119 315 90
Cuts at: 2068 2254 2422 2583 2706 2776 2827 3037 3104
Size: 186 168 161 123 70 51 210 67
Cuts at: 3104 3272 4755 4872
Size: 168 1483 117
Fragments arranged by size:
1483 568 463 315 228 210 186 168
168 168 161 123 119 117 117 90
70 67 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 613 1530 2857 2860 4872
Size: 613 917 1327 3 2012
Fragments arranged by size:
2012 1327 917 613 3
EcoO109I rG'GnC_Cy
Cuts at: 0 117 620 621 1295 1296 1638 2157 2585
Size: 117 503 1 674 1 342 519 428
Cuts at: 2585 3273 3460 4548 4872
Size: 688 187 1088 324
Fragments arranged by size:
1088 688 674 519 503 428 342 324
187 117 1 1
EcoRV GAT'ATC
Cuts at: 0 3474 4872
Size: 3474 1398
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 825 1716 4776 4872
Size: 825 891 3060 96
Fragments arranged by size:
3060 891 825 96
FseI GG_CCGG'CC
Cuts at: 0 8 4872
Size: 8 4864
FspI TGC'GCA
Cuts at: 0 709 4589 4872
Size: 709 3880 283
Fragments arranged by size:
3880 709 283
FspAI rTGC'GCAy
Cuts at: 0 709 4589 4872
Size: 709 3880 283
Fragments arranged by size:
3880 709 283
HaeII r_GCGC'y
Cuts at: 0 387 434 926 1241 2472 3796 4649 4872
Size: 387 47 492 315 1231 1324 853 223
Fragments arranged by size:
1324 1231 853 492 387 315 223 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1816 1848 1849 1881 2382 2414 2563 2595
Size: 1816 32 1 32 501 32 149 32
Cuts at: 2595 3559 3591 3965 3972 3997 4004 4872
Size: 964 32 374 7 25 7 868
Fragments arranged by size:
1816 964 868 501 374 149 32 32
32 32 32 25 7 7 1
HincII GTy'rAC
Cuts at: 0 2802 4872
Size: 2802 2070
HindIII A'AGCT_T
Cuts at: 0 1471 4872
Size: 1471 3401
KasI G'GCGC_C
Cuts at: 0 2468 4872
Size: 2468 2404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 539 1746 3152 3801 4872
Size: 296 243 1207 1406 649 1071
Fragments arranged by size:
1406 1207 1071 649 296 243
MscI TGG'CCA
Cuts at: 0 2476 4313 4843 4872
Size: 2476 1837 530 29
Fragments arranged by size:
2476 1837 530 29
MslI CAynn'nnrTG
Cuts at: 0 199 649 1997 2779 3007 3055 3697 3855
Size: 199 450 1348 782 228 48 642 158
Cuts at: 3855 4075 4472 4872
Size: 220 397 400
Fragments arranged by size:
1348 782 642 450 400 397 228 220
199 158 48
MspA1I CmG'CkG
Cuts at: 0 54 1674 1971 2046 2313 2373 3015 3648
Size: 54 1620 297 75 267 60 642 633
Cuts at: 3648 4872
Size: 1224
Fragments arranged by size:
1620 1224 642 633 297 267 75 60
54
NaeI GCC'GGC
Cuts at: 0 6 825 1401 4872
Size: 6 819 576 3471
Fragments arranged by size:
3471 819 576 6
NarI GG'CG_CC
Cuts at: 0 2469 4872
Size: 2469 2403
NcoI C'CATG_G
Cuts at: 0 287 1598 2081 3866 4872
Size: 287 1311 483 1785 1006
Fragments arranged by size:
1785 1311 1006 483 287
NgoMIV G'CCGG_C
Cuts at: 0 4 823 1399 4872
Size: 4 819 576 3473
Fragments arranged by size:
3473 819 576 4
NheI G'CTAG_C
Cuts at: 0 1389 4872
Size: 1389 3483
NspI r_CATG'y
Cuts at: 0 714 4872
Size: 714 4158
PasI CC'CwG_GG
Cuts at: 0 1548 4872
Size: 1548 3324
PflMI CCAn_nnn'nTGG
Cuts at: 0 441 2640 4872
Size: 441 2199 2232
Fragments arranged by size:
2232 2199 441
PfoI T'CCnGG_A
Cuts at: 0 992 1445 3227 4088 4872
Size: 992 453 1782 861 784
Fragments arranged by size:
1782 992 861 784 453
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2833 2865 4872
Size: 2833 32 2007
Fragments arranged by size:
2833 2007 32
PpuMI rG'GwC_Cy
Cuts at: 0 117 1638 2157 4548 4872
Size: 117 1521 519 2391 324
Fragments arranged by size:
2391 1521 519 324 117
PshAI GACnn'nnGTC
Cuts at: 0 2797 4872
Size: 2797 2075
PspOMI G'GGCC_C
Cuts at: 0 620 1097 1295 2585 3459 4872
Size: 620 477 198 1290 874 1413
Fragments arranged by size:
1413 1290 874 620 477 198
PspXI vC'TCGA_Gb
Cuts at: 0 774 4872
Size: 774 4098
PstI C_TGCA'G
Cuts at: 0 1187 2024 2069 2165 2174 2861 2894 2927
Size: 1187 837 45 96 9 687 33 33
Cuts at: 2927 3272 3857 4033 4872
Size: 345 585 176 839
Fragments arranged by size:
1187 839 837 687 585 345 176 96
45 33 33 9
PvuII CAG'CTG
Cuts at: 0 54 2046 3015 4872
Size: 54 1992 969 1857
Fragments arranged by size:
1992 1857 969 54
SacI G_AGCT'C
Cuts at: 0 449 667 2400 4568 4872
Size: 449 218 1733 2168 304
Fragments arranged by size:
2168 1733 449 304 218
SapI GCTCTTCn'nnn_
Cuts at: 0 1675 1769 4872
Size: 1675 94 3103
Fragments arranged by size:
3103 1675 94
SbfI CC_TGCA'GG
Cuts at: 0 2861 4872
Size: 2861 2011
ScaI AGT'ACT
Cuts at: 0 4027 4872
Size: 4027 845
SexAI A'CCwGG_T
Cuts at: 0 728 4872
Size: 728 4144
SfcI C'TryA_G
Cuts at: 0 963 1183 1248 1279 2020 2065 2161 2170
Size: 963 220 65 31 741 45 96 9
Cuts at: 2170 2242 2857 2890 2923 3268 3853 4029 4872
Size: 72 615 33 33 345 585 176 843
Fragments arranged by size:
963 843 741 615 585 345 220 176
96 72 65 45 33 33 31 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2278 4319 4872
Size: 2278 2041 553
Fragments arranged by size:
2278 2041 553
SfoI GGC'GCC
Cuts at: 0 2470 4872
Size: 2470 2402
SmaI CCC'GGG
Cuts at: 0 104 3601 4625 4872
Size: 104 3497 1024 247
Fragments arranged by size:
3497 1024 247 104
SmlI C'TyrA_G
Cuts at: 0 774 1987 2203 2359 2740 3112 3626 4872
Size: 774 1213 216 156 381 372 514 1246
Fragments arranged by size:
1246 1213 774 514 381 372 216 156
StuI AGG'CCT
Cuts at: 0 139 4872
Size: 139 4733
StyI C'CwwG_G
Cuts at: 0 287 869 905 1394 1598 2081 2273 3026
Size: 287 582 36 489 204 483 192 753
Cuts at: 3026 3616 3830 3866 4385 4872
Size: 590 214 36 519 487
Fragments arranged by size:
753 590 582 519 489 487 483 287
214 204 192 36 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 661 733 1067 1372 1540 2538 3686 3956
Size: 661 72 334 305 168 998 1148 270
Cuts at: 3956 4360 4590 4710 4872
Size: 404 230 120 162
Fragments arranged by size:
1148 998 661 404 334 305 270 230
168 162 120 72
TatI w'GTAC_w
Cuts at: 0 4025 4872
Size: 4025 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 496 528 4588 4620 4872
Size: 496 32 4060 32 252
Fragments arranged by size:
4060 496 252 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 154 2776 3571 4872
Size: 154 2622 795 1301
Fragments arranged by size:
2622 1301 795 154
XbaI T'CTAG_A
Cuts at: 0 1476 4872
Size: 1476 3396
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1638 2802 3012 3033 3897 4872
Size: 1638 1164 210 21 864 975
Fragments arranged by size:
1638 1164 975 864 210 21
XhoI C'TCGA_G
Cuts at: 0 774 3626 4872
Size: 774 2852 1246
Fragments arranged by size:
2852 1246 774
XmaI C'CCGG_G
Cuts at: 0 102 3599 4623 4872
Size: 102 3497 1024 249
Fragments arranged by size:
3497 1024 249 102
ZraI GAC'GTC
Cuts at: 0 3641 4872
Size: 3641 1231
Enzymes that do cut:
AatII AcuI AfeI AflII AleI AlfI AloI AlwNI
ApaI ApaLI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SmaI SmlI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflIII AgeI AhdI ApoI
AscI AseI AsiSI BciVI BfrBI BsaAI BsaWI BsiWI
BspEI BsrGI BssHII BstBI BstZ17I CspCI DraI EagI
EcoRI HpaI KpnI MfeI MluI NdeI NotI NruI
NsiI PacI PciI PmeI PmlI PsiI PsrI PvuI
RsrII SacII SalI SanDI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI XmnI