Restriction map for
pF1KSDA0463
(Linear) (Six-base) MAPSORT of: KIAA0463.seq Check: 9039 from: 1 to: 5727
KIAA0463.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1848 2113 2525 2718 3340 5727
Size: 1848 265 412 193 622 2387
Fragments arranged by size:
2387 1848 622 412 265 193
AatII G_ACGT'C
Cuts at: 0 1217 4894 5727
Size: 1217 3677 833
Fragments arranged by size:
3677 1217 833
AccI GT'mk_AC
Cuts at: 0 803 1585 3005 3116 4924 5727
Size: 803 782 1420 111 1808 803
Fragments arranged by size:
1808 1420 803 803 782 111
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 716 3556 4120 4902 5727
Size: 716 2840 564 782 825
Fragments arranged by size:
2840 825 782 716 564
AfeI AGC'GCT
Cuts at: 0 1522 5166 5727
Size: 1522 3644 561
Fragments arranged by size:
3644 1522 561
AflII C'TTAA_G
Cuts at: 0 2209 5727
Size: 2209 3518
AflIII A'CryG_T
Cuts at: 0 923 1649 5189 5727
Size: 923 726 3540 538
Fragments arranged by size:
3540 923 726 538
AhdI GACnn_n'nnGTC
Cuts at: 0 318 666 5727
Size: 318 348 5061
Fragments arranged by size:
5061 348 318
AleI CACnn'nnGTG
Cuts at: 0 604 2896 2956 5242 5727
Size: 604 2292 60 2286 485
Fragments arranged by size:
2292 2286 604 485 60
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 376 478 5727
Size: 376 102 5249
Fragments arranged by size:
5249 376 102
AlwNI CAG_nnn'CTG
Cuts at: 0 1281 1759 2433 2576 4339 5679 5727
Size: 1281 478 674 143 1763 1340 48
Fragments arranged by size:
1763 1340 1281 674 478 143 48
ApaI G_GGCC'C
Cuts at: 0 75 1898 4462 4959 5646 5727
Size: 75 1823 2564 497 687 81
Fragments arranged by size:
2564 1823 687 497 81 75
ApaLI G'TGCA_C
Cuts at: 0 1221 2538 5152 5727
Size: 1221 1317 2614 575
Fragments arranged by size:
2614 1317 1221 575
ApoI r'AATT_y
Cuts at: 0 3274 5727
Size: 3274 2453
AseI AT'TA_AT
Cuts at: 0 5705 5727
Size: 5705 22
AvaI C'yCGr_G
Cuts at: 0 68 656 823 947 2938 3167 3836 4023
Size: 68 588 167 124 1991 229 669 187
Cuts at: 4023 4727 4952 5727
Size: 704 225 775
Fragments arranged by size:
1991 775 704 669 588 229 225 187
167 124 68
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3981 4014 5727
Size: 3981 33 1713
Fragments arranged by size:
3981 1713 33
BamHI G'GATC_C
Cuts at: 0 3263 5727
Size: 3263 2464
BanI G'GyrC_C
Cuts at: 0 541 1151 1230 2157 2739 3339 4709 5101
Size: 541 610 79 927 582 600 1370 392
Cuts at: 5101 5727
Size: 626
Fragments arranged by size:
1370 927 626 610 600 582 541 392
79
BanII G_rGCy'C
Cuts at: 0 75 1193 1612 1898 3126 3932 4462 4959
Size: 75 1118 419 286 1228 806 530 497
Cuts at: 4959 5636 5646 5727
Size: 677 10 81
Fragments arranged by size:
1228 1118 806 677 530 497 419 286
81 75 10
BbeI G_GCGC'C
Cuts at: 0 1155 1234 4713 5727
Size: 1155 79 3479 1014
Fragments arranged by size:
3479 1155 1014 79
BbvCI CC'TCA_GC
Cuts at: 0 672 1954 5727
Size: 672 1282 3773
Fragments arranged by size:
3773 1282 672
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1084 1118 5727
Size: 1084 34 4609
Fragments arranged by size:
4609 1084 34
BclI T'GATC_A
Cuts at: 0 1515 4964 5727
Size: 1515 3449 763
Fragments arranged by size:
3449 1515 763
BfrBI ATG'CAT
Cuts at: 0 4800 5727
Size: 4800 927
BglI GCCn_nnn'nGGC
Cuts at: 0 2249 4229 5727
Size: 2249 1980 1498
Fragments arranged by size:
2249 1980 1498
BglII A'GATC_T
Cuts at: 0 2171 5069 5600 5727
Size: 2171 2898 531 127
Fragments arranged by size:
2898 2171 531 127
BlpI GC'TnA_GC
Cuts at: 0 1777 2777 3487 3926 5727
Size: 1777 1000 710 439 1801
Fragments arranged by size:
1801 1777 1000 710 439
Bme1580I G_kGCm'C
Cuts at: 0 75 544 1225 1641 1898 2019 2162 2542
Size: 75 469 681 416 257 121 143 380
Cuts at: 2542 2744 4462 4667 4959 5104 5156 5592 5646
Size: 202 1718 205 292 145 52 436 54
Cuts at: 5646 5727
Size: 81
Fragments arranged by size:
1718 681 469 436 416 380 292 257
205 202 145 143 121 81 75 54
52
BmrI ACTGGGnnnn_n'
Cuts at: 0 4585 5304 5727
Size: 4585 719 423
Fragments arranged by size:
4585 719 423
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1479 1814 5622 5727
Size: 1479 335 3808 105
Fragments arranged by size:
3808 1479 335 105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 878 1207 1280 1963 2301 3518 3587
Size: 97 781 329 73 683 338 1217 69
Cuts at: 3587 3907 3916 4078 4180 4315 5727
Size: 320 9 162 102 135 1412
Fragments arranged by size:
1412 1217 781 683 338 329 320 162
135 102 97 73 69 9
Bpu10I CC'TnA_GC
Cuts at: 0 672 1606 1954 3972 4498 5727
Size: 672 934 348 2018 526 1229
Fragments arranged by size:
2018 1229 934 672 526 348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1456 2473 3841 4243 5727
Size: 1456 1017 1368 402 1484
Fragments arranged by size:
1484 1456 1368 1017 402
BsaI GGTCTCn'nnnn_
Cuts at: 0 653 756 820 854 1467 3598 3625 5128
Size: 653 103 64 34 613 2131 27 1503
Cuts at: 5128 5727
Size: 599
Fragments arranged by size:
2131 1503 653 613 599 103 64 34
27
BsaAI yAC'GTr
Cuts at: 0 924 3690 4086 5727
Size: 924 2766 396 1641
Fragments arranged by size:
2766 1641 924 396
BsaHI Gr'CG_yC
Cuts at: 0 1152 1214 1231 4710 4891 5348 5727
Size: 1152 62 17 3479 181 457 379
Fragments arranged by size:
3479 1152 457 379 181 62 17
BsaWI w'CCGG_w
Cuts at: 0 2498 4325 5727
Size: 2498 1827 1402
Fragments arranged by size:
2498 1827 1402
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1639 1669 5410 5440 5727
Size: 1639 30 3741 30 287
Fragments arranged by size:
3741 1639 287 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 13 702 2182 3778 3781 4842 4902 5644
Size: 13 689 1480 1596 3 1061 60 742
Cuts at: 5644 5727
Size: 83
Fragments arranged by size:
1596 1480 1061 742 689 83 60 13
3
BseYI C'CCAG_C
Cuts at: 0 135 1203 1741 2150 2228 2462 2734 2986
Size: 135 1068 538 409 78 234 272 252
Cuts at: 2986 3635 4112 4673 5409 5458 5471 5508 5727
Size: 649 477 561 736 49 13 37 219
Fragments arranged by size:
1068 736 649 561 538 477 409 272
252 234 219 135 78 49 37 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 927 1627 2070 2477 2541 2730 2746
Size: 364 563 700 443 407 64 189 16
Cuts at: 2746 4366 5545 5727
Size: 1620 1179 182
Fragments arranged by size:
1620 1179 700 563 443 407 364 189
182 64 16
BsiEI CG_ry'CG
Cuts at: 0 2499 3120 3776 4191 4681 5727
Size: 2499 621 656 415 490 1046
Fragments arranged by size:
2499 1046 656 621 490 415
BsiHKAI G_wGCw'C
Cuts at: 0 7 365 515 1193 1225 1472 1612 1657
Size: 7 358 150 678 32 247 140 45
Cuts at: 1657 1766 2542 2611 3596 3932 5156 5405 5636
Size: 109 776 69 985 336 1224 249 231
Cuts at: 5636 5727
Size: 91
Fragments arranged by size:
1224 985 776 678 358 336 249 247
231 150 140 109 91 69 45 32
7
BsmI GAATG_Cn'
Cuts at: 0 5538 5727
Size: 5538 189
BsmBI CGTCTCn'nnnn_
Cuts at: 0 25 5727
Size: 25 5702
Bsp1286I G_dGCh'C
Cuts at: 0 7 75 365 515 544 1193 1225 1472
Size: 7 68 290 150 29 649 32 247
Cuts at: 1472 1612 1641 1657 1766 1898 2019 2162 2542
Size: 140 29 16 109 132 121 143 380
Cuts at: 2542 2611 2744 3126 3596 3932 4462 4667 4959
Size: 69 133 382 470 336 530 205 292
Cuts at: 4959 5104 5156 5405 5592 5636 5646 5727
Size: 145 52 249 187 44 10 81
Fragments arranged by size:
649 530 470 382 380 336 292 290
249 247 205 187 150 145 143 140
133 132 121 109 81 69 68 52
44 32 29 29 16 10 7
BspEI T'CCGG_A
Cuts at: 0 4325 5727
Size: 4325 1402
BspHI T'CATG_A
Cuts at: 0 2091 2867 4214 5727
Size: 2091 776 1347 1513
Fragments arranged by size:
2091 1513 1347 776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1848 2113 2525 2718 3031 3149 3340 5727
Size: 1848 265 412 193 313 118 191 2387
Fragments arranged by size:
2387 1848 412 313 265 193 191 118
BsrBI CCG'CTC
Cuts at: 0 92 2299 3971 5727
Size: 92 2207 1672 1756
Fragments arranged by size:
2207 1756 1672 92
BsrDI GCAATG_nn'
Cuts at: 0 3197 3815 5727
Size: 3197 618 1912
Fragments arranged by size:
3197 1912 618
BsrFI r'CCGG_y
Cuts at: 0 142 1769 2968 3775 4219 5405 5727
Size: 142 1627 1199 807 444 1186 322
Fragments arranged by size:
1627 1199 1186 807 444 322 142
BsrGI T'GTAC_A
Cuts at: 0 1316 5727
Size: 1316 4411
BssSI C'ACGA_G
Cuts at: 0 2809 5727
Size: 2809 2918
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2084 5727
Size: 2084 3643
BstEII G'GTnAC_C
Cuts at: 0 1551 2958 5026 5041 5727
Size: 1551 1407 2068 15 686
Fragments arranged by size:
2068 1551 1407 686 15
BstXI CCAn_nnnn'nTGG
Cuts at: 0 285 2353 2980 3963 5727
Size: 285 2068 627 983 1764
Fragments arranged by size:
2068 1764 983 627 285
BstYI r'GATC_y
Cuts at: 0 725 2171 3263 4586 5069 5600 5727
Size: 725 1446 1092 1323 483 531 127
Fragments arranged by size:
1446 1323 1092 725 531 483 127
BstZ17I GTA'TAC
Cuts at: 0 1586 4925 5727
Size: 1586 3339 802
Fragments arranged by size:
3339 1586 802
Bsu36I CC'TnA_GG
Cuts at: 0 833 2426 5727
Size: 833 1593 3301
Fragments arranged by size:
3301 1593 833
BtgI C'CryG_G
Cuts at: 0 1437 1565 2378 2679 3353 5022 5567 5727
Size: 1437 128 813 301 674 1669 545 160
Fragments arranged by size:
1669 1437 813 674 545 301 160 128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2757 3753 5481 5727
Size: 2757 996 1728 246
Fragments arranged by size:
2757 1728 996 246
BtsI GCAGTG_nn'
Cuts at: 0 1739 2019 4694 5727
Size: 1739 280 2675 1033
Fragments arranged by size:
2675 1739 1033 280
ClaI AT'CG_AT
Cuts at: 0 1244 1695 4283 5727
Size: 1244 451 2588 1444
Fragments arranged by size:
2588 1444 1244 451
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1280 1315 4746 4781 5727
Size: 1280 35 3431 35 946
Fragments arranged by size:
3431 1280 946 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 2724 5568 5727
Size: 2724 2844 159
Fragments arranged by size:
2844 2724 159
DrdI GACnn_nn'nnGTC
Cuts at: 0 2437 3169 4042 5727
Size: 2437 732 873 1685
Fragments arranged by size:
2437 1685 873 732
EaeI y'GGCC_r
Cuts at: 0 842 989 2241 2375 2970 3207 3773 4678
Size: 842 147 1252 134 595 237 566 905
Cuts at: 4678 5090 5177 5727
Size: 412 87 550
Fragments arranged by size:
1252 905 842 595 566 550 412 237
147 134 87
EagI C'GGCC_G
Cuts at: 0 3773 4678 5727
Size: 3773 905 1049
Fragments arranged by size:
3773 1049 905
EarI CTCTTCn'nnn_
Cuts at: 0 302 484 595 871 4423 5727
Size: 302 182 111 276 3552 1304
Fragments arranged by size:
3552 1304 302 276 182 111
EcoICRI GAG'CTC
Cuts at: 0 1191 1610 3930 5634 5727
Size: 1191 419 2320 1704 93
Fragments arranged by size:
2320 1704 1191 419 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 716 878 1207 1280 1963 2301 3518
Size: 97 619 162 329 73 683 338 1217
Cuts at: 3518 3556 3587 3907 3916 4078 4120 4180 4315
Size: 38 31 320 9 162 42 60 135
Cuts at: 4315 4902 5727
Size: 587 825
Fragments arranged by size:
1217 825 683 619 587 338 329 320
162 162 135 97 73 60 42 38
31 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 442 973 1843 4501 4917 5650 5727
Size: 442 531 870 2658 416 733 77
Fragments arranged by size:
2658 870 733 531 442 416 77
EcoO109I rG'GnC_Cy
Cuts at: 0 72 1305 1560 1617 2292 2430 2935 4095
Size: 72 1233 255 57 675 138 505 1160
Cuts at: 4095 4458 4459 4956 5642 5643 5727
Size: 363 1 497 686 1 84
Fragments arranged by size:
1233 1160 686 675 505 497 363 255
138 84 72 57 1 1
EcoRV GAT'ATC
Cuts at: 0 3942 5727
Size: 3942 1785
FspI TGC'GCA
Cuts at: 0 4404 5727
Size: 4404 1323
HaeII r_GCGC'y
Cuts at: 0 1155 1234 1524 2051 4713 5168 5727
Size: 1155 79 290 527 2662 455 559
Fragments arranged by size:
2662 1155 559 527 455 290 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 304 336 652 684 694 726
Size: 76 32 196 32 316 32 10 32
Cuts at: 726 1253 1285 2660 2692 3512 3544 3867 3899
Size: 527 32 1375 32 820 32 323 32
Cuts at: 3899 4788 4820 5410 5442 5727
Size: 889 32 590 32 285
Fragments arranged by size:
1375 889 820 590 527 323 316 285
196 76 32 32 32 32 32 32
32 32 32 32 10
HincII GTy'rAC
Cuts at: 0 384 531 2043 3117 3953 4560 5727
Size: 384 147 1512 1074 836 607 1167
Fragments arranged by size:
1512 1167 1074 836 607 384 147
HindIII A'AGCT_T
Cuts at: 0 3917 5727
Size: 3917 1810
KasI G'GCGC_C
Cuts at: 0 1151 1230 4709 5727
Size: 1151 79 3479 1018
Fragments arranged by size:
3479 1151 1018 79
MfeI C'AATT_G
Cuts at: 0 4362 5727
Size: 4362 1365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2636 5453 5727
Size: 2636 2817 274
Fragments arranged by size:
2817 2636 274
MscI TGG'CCA
Cuts at: 0 844 991 3209 5092 5179 5727
Size: 844 147 2218 1883 87 548
Fragments arranged by size:
2218 1883 844 548 147 87
MslI CAynn'nnrTG
Cuts at: 0 283 487 604 2896 2956 4795 5242 5727
Size: 283 204 117 2292 60 1839 447 485
Fragments arranged by size:
2292 1839 485 447 283 204 117 60
MspA1I CmG'CkG
Cuts at: 0 856 1920 1953 2057 2154 2466 2525 4266
Size: 856 1064 33 104 97 312 59 1741
Cuts at: 4266 4677 5676 5727
Size: 411 999 51
Fragments arranged by size:
1741 1064 999 856 411 312 104 97
59 51 33
NaeI GCC'GGC
Cuts at: 0 144 3777 5727
Size: 144 3633 1950
Fragments arranged by size:
3633 1950 144
NarI GG'CG_CC
Cuts at: 0 1152 1231 4710 5727
Size: 1152 79 3479 1017
Fragments arranged by size:
3479 1152 1017 79
NcoI C'CATG_G
Cuts at: 0 1437 2679 5727
Size: 1437 1242 3048
Fragments arranged by size:
3048 1437 1242
NgoMIV G'CCGG_C
Cuts at: 0 142 3775 5727
Size: 142 3633 1952
Fragments arranged by size:
3633 1952 142
NsiI A_TGCA'T
Cuts at: 0 4802 5727
Size: 4802 925
NspI r_CATG'y
Cuts at: 0 150 1653 5193 5583 5727
Size: 150 1503 3540 390 144
Fragments arranged by size:
3540 1503 390 150 144
PasI CC'CwG_GG
Cuts at: 0 1281 2758 5727
Size: 1281 1477 2969
Fragments arranged by size:
2969 1477 1281
PciI A'CATG_T
Cuts at: 0 1649 5189 5727
Size: 1649 3540 538
Fragments arranged by size:
3540 1649 538
PflMI CCAn_nnn'nTGG
Cuts at: 0 1281 2576 5727
Size: 1281 1295 3151
Fragments arranged by size:
3151 1295 1281
PfoI T'CCnGG_A
Cuts at: 0 1493 1864 2114 4885 5727
Size: 1493 371 250 2771 842
Fragments arranged by size:
2771 1493 842 371 250
PmlI CAC'GTG
Cuts at: 0 3690 4086 5727
Size: 3690 396 1641
Fragments arranged by size:
3690 1641 396
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5125 5157 5727
Size: 5125 32 570
Fragments arranged by size:
5125 570 32
PpuMI rG'GwC_Cy
Cuts at: 0 1560 1617 2292 2430 2935 5727
Size: 1560 57 675 138 505 2792
Fragments arranged by size:
2792 1560 675 505 138 57
PsiI TTA'TAA
Cuts at: 0 5687 5727
Size: 5687 40
PspOMI G'GGCC_C
Cuts at: 0 71 1894 4458 4955 5642 5727
Size: 71 1823 2564 497 687 85
Fragments arranged by size:
2564 1823 687 497 85 71
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2326 2358 5727
Size: 2326 32 3369
Fragments arranged by size:
3369 2326 32
PstI C_TGCA'G
Cuts at: 0 355 977 2014 3146 4169 4229 5111 5727
Size: 355 622 1037 1132 1023 60 882 616
Fragments arranged by size:
1132 1037 1023 882 622 616 355 60
PvuII CAG'CTG
Cuts at: 0 1953 2154 4266 5727
Size: 1953 201 2112 1461
Fragments arranged by size:
2112 1953 1461 201
SacI G_AGCT'C
Cuts at: 0 1193 1612 3932 5636 5727
Size: 1193 419 2320 1704 91
Fragments arranged by size:
2320 1704 1193 419 91
SalI G'TCGA_C
Cuts at: 0 3115 5727
Size: 3115 2612
SanDI GG'GwC_CC
Cuts at: 0 1560 1617 2292 5727
Size: 1560 57 675 3435
Fragments arranged by size:
3435 1560 675 57
SapI GCTCTTCn'nnn_
Cuts at: 0 595 5727
Size: 595 5132
SbfI CC_TGCA'GG
Cuts at: 0 977 4229 5727
Size: 977 3252 1498
Fragments arranged by size:
3252 1498 977
SfcI C'TryA_G
Cuts at: 0 351 973 1156 1371 1948 2010 3142 4165
Size: 351 622 183 215 577 62 1132 1023
Cuts at: 4165 4225 5107 5727
Size: 60 882 620
Fragments arranged by size:
1132 1023 882 622 620 577 351 215
183 62 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 4229 5727
Size: 4229 1498
SfoI GGC'GCC
Cuts at: 0 1153 1232 4711 5727
Size: 1153 79 3479 1016
Fragments arranged by size:
3479 1153 1016 79
SmaI CCC'GGG
Cuts at: 0 70 949 4025 4729 4954 5727
Size: 70 879 3076 704 225 773
Fragments arranged by size:
3076 879 773 704 225 70
SmlI C'TyrA_G
Cuts at: 0 1471 2209 2488 3836 3856 4258 5727
Size: 1471 738 279 1348 20 402 1469
Fragments arranged by size:
1471 1469 1348 738 402 279 20
SphI G_CATG'C
Cuts at: 0 150 5727
Size: 150 5577
SspI AAT'ATT
Cuts at: 0 2133 5727
Size: 2133 3594
StuI AGG'CCT
Cuts at: 0 1018 1681 5727
Size: 1018 663 4046
Fragments arranged by size:
4046 1018 663
StyI C'CwwG_G
Cuts at: 0 213 1226 1437 1898 1961 2679 2979 3087
Size: 213 1013 211 461 63 718 300 108
Cuts at: 3087 3259 3902 4713 5096 5447 5727
Size: 172 643 811 383 351 280
Fragments arranged by size:
1013 811 718 643 461 383 351 300
280 213 211 172 108 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 108 3134 5277 5463 5727
Size: 108 3026 2143 186 264
Fragments arranged by size:
3026 2143 264 186 108
TatI w'GTAC_w
Cuts at: 0 1044 1316 2888 3152 4532 5186 5727
Size: 1044 272 1572 264 1380 654 541
Fragments arranged by size:
1572 1380 1044 654 541 272 264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 505 537 1204 1236 1383 1415 4690 4722
Size: 505 32 667 32 147 32 3275 32
Cuts at: 4722 5727
Size: 1005
Fragments arranged by size:
3275 1005 667 505 147 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 636 2640 2874 4335 5727
Size: 636 2004 234 1461 1392
Fragments arranged by size:
2004 1461 1392 636 234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1563 5727
Size: 1563 4164
XhoI C'TCGA_G
Cuts at: 0 3836 5727
Size: 3836 1891
XmaI C'CCGG_G
Cuts at: 0 68 947 4023 4727 4952 5727
Size: 68 879 3076 704 225 775
Fragments arranged by size:
3076 879 775 704 225 68
ZraI GAC'GTC
Cuts at: 0 1215 4892 5727
Size: 1215 3677 835
Fragments arranged by size:
3677 1215 835
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI
BaeI BamHI BanI BanII BbeI BbvCI BcgI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I HincII
HindIII KasI MfeI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PsiI PspOMI PsrI PstI
PvuII SacI SalI SanDI SapI SbfI SfcI SfiI
SfoI SmaI SmlI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AgeI AloI AscI AsiSI AvrII BbsI
BciVI BmgBI BmtI BsaBI BsiWI BssHII BstBI DraI
EciI EcoRI FalI FseI FspAI HpaI KpnI MluI
NdeI NheI NotI NruI PacI PmeI PshAI PspXI
PvuI RsrII SacII ScaI SexAI SgfI SgrAI SnaBI
SpeI SrfI SwaI XbaI XmnI