Restriction map for
pF1KA0464
(Linear) (Six-base) MAPSORT of: KIAA0464.seq Check: 2280 from: 1 to: 972
KIAA0464.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 354 390 972
Size: 274 80 36 582
Fragments arranged by size:
582 274 80 36
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 536 972
Size: 536 436
ApoI r'AATT_y
Cuts at: 0 679 972
Size: 679 293
AvaI C'yCGr_G
Cuts at: 0 383 972
Size: 383 589
BamHI G'GATC_C
Cuts at: 0 50 972
Size: 50 922
BanI G'GyrC_C
Cuts at: 0 391 972
Size: 391 581
BanII G_rGCy'C
Cuts at: 0 767 972
Size: 767 205
BbvCI CC'TCA_GC
Cuts at: 0 931 972
Size: 931 41
BciVI GTATCCnnnnn_n'
Cuts at: 0 164 972
Size: 164 808
BglI GCCn_nnn'nGGC
Cuts at: 0 604 613 972
Size: 604 9 359
Fragments arranged by size:
604 359 9
BglII A'GATC_T
Cuts at: 0 365 972
Size: 365 607
BlpI GC'TnA_GC
Cuts at: 0 496 972
Size: 496 476
Bme1580I G_kGCm'C
Cuts at: 0 41 832 972
Size: 41 791 140
Fragments arranged by size:
791 140 41
BmgBI CAC'GTC
Cuts at: 0 123 284 972
Size: 123 161 688
Fragments arranged by size:
688 161 123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 624 972
Size: 624 348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 803 856 868 972
Size: 614 189 53 12 104
Fragments arranged by size:
614 189 104 53 12
Bpu10I CC'TnA_GC
Cuts at: 0 567 931 972
Size: 567 364 41
Fragments arranged by size:
567 364 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 566 972
Size: 566 406
BsaAI yAC'GTr
Cuts at: 0 108 972
Size: 108 864
BsaHI Gr'CG_yC
Cuts at: 0 71 972
Size: 71 901
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 230 260 724 754 972
Size: 230 30 464 30 218
Fragments arranged by size:
464 230 218 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 640 859 871 919 928 972
Size: 640 219 12 48 9 44
Fragments arranged by size:
640 219 48 44 12 9
BsmI GAATG_Cn'
Cuts at: 0 525 972
Size: 525 447
Bsp1286I G_dGCh'C
Cuts at: 0 41 767 832 972
Size: 41 726 65 140
Fragments arranged by size:
726 140 65 41
BspHI T'CATG_A
Cuts at: 0 345 972
Size: 345 627
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 53 972
Size: 53 919
BstAPI GCAn_nnn'nTGC
Cuts at: 0 45 972
Size: 45 927
BstXI CCAn_nnnn'nTGG
Cuts at: 0 538 592 965 972
Size: 538 54 373 7
Fragments arranged by size:
538 373 54 7
BstYI r'GATC_y
Cuts at: 0 50 329 365 972
Size: 50 279 36 607
Fragments arranged by size:
607 279 50 36
BtgI C'CryG_G
Cuts at: 0 611 972
Size: 611 361
BtsI GCAGTG_nn'
Cuts at: 0 617 972
Size: 617 355
ClaI AT'CG_AT
Cuts at: 0 638 972
Size: 638 334
DraI TTT'AAA
Cuts at: 0 186 423 972
Size: 186 237 549
Fragments arranged by size:
549 237 186
DrdI GACnn_nn'nnGTC
Cuts at: 0 843 972
Size: 843 129
EaeI y'GGCC_r
Cuts at: 0 528 614 972
Size: 528 86 358
Fragments arranged by size:
528 358 86
EarI CTCTTCn'nnn_
Cuts at: 0 430 972
Size: 430 542
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 354 390 614 803 856 868 972
Size: 274 80 36 224 189 53 12 104
Fragments arranged by size:
274 224 189 104 80 53 36 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 783 972
Size: 783 189
EcoO109I rG'GnC_Cy
Cuts at: 0 315 605 651 972
Size: 315 290 46 321
Fragments arranged by size:
321 315 290 46
EcoRI G'AATT_C
Cuts at: 0 679 972
Size: 679 293
MscI TGG'CCA
Cuts at: 0 530 972
Size: 530 442
MslI CAynn'nnrTG
Cuts at: 0 963 972
Size: 963 9
MspA1I CmG'CkG
Cuts at: 0 843 972
Size: 843 129
PfoI T'CCnGG_A
Cuts at: 0 674 972
Size: 674 298
PpuMI rG'GwC_Cy
Cuts at: 0 315 651 972
Size: 315 336 321
Fragments arranged by size:
336 321 315
PstI C_TGCA'G
Cuts at: 0 506 624 972
Size: 506 118 348
Fragments arranged by size:
506 348 118
SapI GCTCTTCn'nnn_
Cuts at: 0 430 972
Size: 430 542
SfcI C'TryA_G
Cuts at: 0 324 502 620 710 972
Size: 324 178 118 90 262
Fragments arranged by size:
324 262 178 118 90
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 613 972
Size: 613 359
SmlI C'TyrA_G
Cuts at: 0 383 972
Size: 383 589
SnaBI TAC'GTA
Cuts at: 0 108 972
Size: 108 864
StuI AGG'CCT
Cuts at: 0 475 824 972
Size: 475 349 148
Fragments arranged by size:
475 349 148
StyI C'CwwG_G
Cuts at: 0 127 743 972
Size: 127 616 229
Fragments arranged by size:
616 229 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 798 972
Size: 798 174
TatI w'GTAC_w
Cuts at: 0 17 972
Size: 17 955
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 230 262 268 300 724 756 972
Size: 230 32 6 32 424 32 216
Fragments arranged by size:
424 230 216 32 32 32 6
XbaI T'CTAG_A
Cuts at: 0 702 972
Size: 702 270
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 141 972
Size: 141 831
XhoI C'TCGA_G
Cuts at: 0 383 972
Size: 383 589
XmnI GAAnn'nnTTC
Cuts at: 0 367 972
Size: 367 605
Enzymes that do cut:
AcuI AlfI ApoI AvaI BamHI BanI BanII BbvCI
BciVI BglI BglII BlpI Bme1580I BmgBI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BsmI Bsp1286I
BspHI BspMI BstAPI BstXI BstYI BtgI BtsI ClaI
DraI DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
MscI MslI MspA1I PfoI PpuMI PstI SapI SfcI
SfiI SmlI SnaBI StuI StyI TaqII TatI TstI
XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BbeI BbsI BcgI BclI
BfrBI BmrI BmtI BpuEI BsaBI BsaWI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
CspCI DraIII EagI EciI EcoICRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfoI SgfI SgrAI SmaI
SpeI SphI SrfI SspI SwaI Tth111I XmaI ZraI