Restriction map for
pF1KSDA0466
(Linear) (Six-base) MAPSORT of: KIAA0466.seq Check: 8880 from: 1 to: 3582
KIAA0466.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 713 1452 2031 2850 3582
Size: 713 739 579 819 732
Fragments arranged by size:
819 739 732 713 579
Acc65I G'GTAC_C
Cuts at: 0 3278 3582
Size: 3278 304
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 1102 2212 2680 3582
Size: 313 789 1110 468 902
Fragments arranged by size:
1110 902 789 468 313
AflII C'TTAA_G
Cuts at: 0 1045 3582
Size: 1045 2537
AleI CACnn'nnGTG
Cuts at: 0 2071 3582
Size: 2071 1511
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 461 3582
Size: 461 3121
AlwNI CAG_nnn'CTG
Cuts at: 0 462 645 3118 3582
Size: 462 183 2473 464
Fragments arranged by size:
2473 464 462 183
ApaI G_GGCC'C
Cuts at: 0 3112 3582
Size: 3112 470
ApoI r'AATT_y
Cuts at: 0 159 727 838 1015 1162 3582
Size: 159 568 111 177 147 2420
Fragments arranged by size:
2420 568 177 159 147 111
AvaI C'yCGr_G
Cuts at: 0 335 1796 3582
Size: 335 1461 1786
Fragments arranged by size:
1786 1461 335
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 73 106 3582
Size: 73 33 3476
Fragments arranged by size:
3476 73 33
BamHI G'GATC_C
Cuts at: 0 752 759 3582
Size: 752 7 2823
Fragments arranged by size:
2823 752 7
BanI G'GyrC_C
Cuts at: 0 1399 3278 3489 3582
Size: 1399 1879 211 93
Fragments arranged by size:
1879 1399 211 93
BanII G_rGCy'C
Cuts at: 0 106 494 2371 3112 3548 3582
Size: 106 388 1877 741 436 34
Fragments arranged by size:
1877 741 436 388 106 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 2200 3378 3582
Size: 2200 1178 204
Fragments arranged by size:
2200 1178 204
BciVI GTATCCnnnnn_n'
Cuts at: 0 3571 3582
Size: 3571 11
BfrBI ATG'CAT
Cuts at: 0 905 3582
Size: 905 2677
BglI GCCn_nnn'nGGC
Cuts at: 0 2783 3134 3582
Size: 2783 351 448
Fragments arranged by size:
2783 448 351
BglII A'GATC_T
Cuts at: 0 275 324 1085 3582
Size: 275 49 761 2497
Fragments arranged by size:
2497 761 275 49
BlpI GC'TnA_GC
Cuts at: 0 22 2365 2455 3100 3582
Size: 22 2343 90 645 482
Fragments arranged by size:
2343 645 482 90 22
Bme1580I G_kGCm'C
Cuts at: 0 1765 3112 3582
Size: 1765 1347 470
Fragments arranged by size:
1765 1347 470
BmrI ACTGGGnnnn_n'
Cuts at: 0 1434 2774 3582
Size: 1434 1340 808
Fragments arranged by size:
1434 1340 808
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 525 603 2010 2275 3582
Size: 525 78 1407 265 1307
Fragments arranged by size:
1407 1307 525 265 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 600 874 931 1815 2026 2245 2506
Size: 508 92 274 57 884 211 219 261
Cuts at: 2506 2527 2922 3328 3444 3562 3582
Size: 21 395 406 116 118 20
Fragments arranged by size:
884 508 406 395 274 261 219 211
118 116 92 57 21 20
Bpu10I CC'TnA_GC
Cuts at: 0 592 1888 2585 3582
Size: 592 1296 697 997
Fragments arranged by size:
1296 997 697 592
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1204 1288 2628 3582
Size: 1204 84 1340 954
Fragments arranged by size:
1340 1204 954 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 508 2482 2895 3582
Size: 508 1974 413 687
Fragments arranged by size:
1974 687 508 413
BsaAI yAC'GTr
Cuts at: 0 2349 3582
Size: 2349 1233
BsaHI Gr'CG_yC
Cuts at: 0 1448 2618 3582
Size: 1448 1170 964
Fragments arranged by size:
1448 1170 964
BsaWI w'CCGG_w
Cuts at: 0 762 3582
Size: 762 2820
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 384 414 797 827 1663 1693 1813 1843
Size: 384 30 383 30 836 30 120 30
Cuts at: 1843 3283 3313 3582
Size: 1440 30 269
Fragments arranged by size:
1440 836 384 383 269 120 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2251 2254 2368 2413 2776 3055 3058 3061
Size: 2251 3 114 45 363 279 3 3
Cuts at: 3061 3064 3067 3262 3307 3310 3582
Size: 3 3 195 45 3 272
Fragments arranged by size:
2251 363 279 272 195 114 45 45
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 39 254 367 669 2543 2773 2815 2989
Size: 39 215 113 302 1874 230 42 174
Cuts at: 2989 3259 3582
Size: 270 323
Fragments arranged by size:
1874 323 302 270 230 215 174 113
42 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 448 856 1426 1486 2749 3582
Size: 226 222 408 570 60 1263 833
Fragments arranged by size:
1263 833 570 408 226 222 60
BsiEI CG_ry'CG
Cuts at: 0 933 3582
Size: 933 2649
BsiHKAI G_wGCw'C
Cuts at: 0 494 2147 3185 3582
Size: 494 1653 1038 397
Fragments arranged by size:
1653 1038 494 397
BsmI GAATG_Cn'
Cuts at: 0 361 1534 3413 3582
Size: 361 1173 1879 169
Fragments arranged by size:
1879 1173 361 169
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2710 3582
Size: 2710 872
Bsp1286I G_dGCh'C
Cuts at: 0 106 494 1765 2147 2371 3112 3185 3548
Size: 106 388 1271 382 224 741 73 363
Cuts at: 3548 3582
Size: 34
Fragments arranged by size:
1271 741 388 382 363 224 106 73
34
BspEI T'CCGG_A
Cuts at: 0 762 3582
Size: 762 2820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 54 188 713 1289 1452 2009 2031 2487
Size: 54 134 525 576 163 557 22 456
Cuts at: 2487 2850 3582
Size: 363 732
Fragments arranged by size:
732 576 557 525 456 363 163 134
54 22
BsrBI CCG'CTC
Cuts at: 0 2589 2936 3087 3582
Size: 2589 347 151 495
Fragments arranged by size:
2589 495 347 151
BsrDI GCAATG_nn'
Cuts at: 0 132 1257 3582
Size: 132 1125 2325
Fragments arranged by size:
2325 1125 132
BsrFI r'CCGG_y
Cuts at: 0 888 1755 3188 3281 3582
Size: 888 867 1433 93 301
Fragments arranged by size:
1433 888 867 301 93
BstEII G'GTnAC_C
Cuts at: 0 66 1665 1785 3582
Size: 66 1599 120 1797
Fragments arranged by size:
1797 1599 120 66
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2004 3435 3582
Size: 2004 1431 147
Fragments arranged by size:
2004 1431 147
BstYI r'GATC_y
Cuts at: 0 275 324 752 759 1085 3582
Size: 275 49 428 7 326 2497
Fragments arranged by size:
2497 428 326 275 49 7
Bsu36I CC'TnA_GG
Cuts at: 0 2449 2977 3582
Size: 2449 528 605
Fragments arranged by size:
2449 605 528
BtgI C'CryG_G
Cuts at: 0 221 264 575 809 980 1235 2885 3582
Size: 221 43 311 234 171 255 1650 697
Fragments arranged by size:
1650 697 311 255 234 221 171 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 957 3582
Size: 957 2625
BtsI GCAGTG_nn'
Cuts at: 0 443 1302 1562 1699 3582
Size: 443 859 260 137 1883
Fragments arranged by size:
1883 859 443 260 137
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 114 149 3582
Size: 114 35 3433
Fragments arranged by size:
3433 114 35
DraIII CAC_nnn'GTG
Cuts at: 0 471 879 3582
Size: 471 408 2703
Fragments arranged by size:
2703 471 408
DrdI GACnn_nn'nnGTC
Cuts at: 0 1807 2872 3582
Size: 1807 1065 710
Fragments arranged by size:
1807 1065 710
EaeI y'GGCC_r
Cuts at: 0 1052 1244 3582
Size: 1052 192 2338
Fragments arranged by size:
2338 1052 192
EarI CTCTTCn'nnn_
Cuts at: 0 234 2050 2713 3023 3379 3582
Size: 234 1816 663 310 356 203
Fragments arranged by size:
1816 663 356 310 234 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 676 1087 1873 1965 1999 2199 3582
Size: 676 411 786 92 34 200 1383
Fragments arranged by size:
1383 786 676 411 200 92 34
EcoICRI GAG'CTC
Cuts at: 0 492 3582
Size: 492 3090
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 508 600 874 931 1102 1815 2026
Size: 313 195 92 274 57 171 713 211
Cuts at: 2026 2212 2245 2506 2527 2680 2922 3328 3444
Size: 186 33 261 21 153 242 406 116
Cuts at: 3444 3562 3582
Size: 118 20
Fragments arranged by size:
713 406 313 274 261 242 211 195
186 171 153 118 116 92 57 33
21 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 649 1099 3582
Size: 649 450 2483
Fragments arranged by size:
2483 649 450
EcoO109I rG'GnC_Cy
Cuts at: 0 82 145 480 985 1278 1725 1826 1991
Size: 82 63 335 505 293 447 101 165
Cuts at: 1991 2060 2244 2286 2741 3582
Size: 69 184 42 455 841
Fragments arranged by size:
841 505 455 447 335 293 184 165
101 82 69 63 42
EcoRI G'AATT_C
Cuts at: 0 727 838 1162 3582
Size: 727 111 324 2420
Fragments arranged by size:
2420 727 324 111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 402 3582
Size: 402 3180
HaeII r_GCGC'y
Cuts at: 0 1590 3582
Size: 1590 1992
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 260 292 744 776 797 829 1170 1202
Size: 260 32 452 32 21 32 341 32
Cuts at: 1202 1813 1845 2303 2335 3283 3315 3582
Size: 611 32 458 32 948 32 267
Fragments arranged by size:
948 611 458 452 341 267 260 32
32 32 32 32 32 32 21
HincII GTy'rAC
Cuts at: 0 816 3582
Size: 816 2766
HindIII A'AGCT_T
Cuts at: 0 2182 3582
Size: 2182 1400
KpnI G_GTAC'C
Cuts at: 0 3282 3582
Size: 3282 300
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2021 3386 3582
Size: 2021 1365 196
Fragments arranged by size:
2021 1365 196
MscI TGG'CCA
Cuts at: 0 1054 1246 3582
Size: 1054 192 2336
Fragments arranged by size:
2336 1054 192
MslI CAynn'nnrTG
Cuts at: 0 448 1234 2071 3582
Size: 448 786 837 1511
Fragments arranged by size:
1511 837 786 448
MspA1I CmG'CkG
Cuts at: 0 60 266 486 2330 2505 2738 2750 2815
Size: 60 206 220 1844 175 233 12 65
Cuts at: 2815 2913 3582
Size: 98 669
Fragments arranged by size:
1844 669 233 220 206 175 98 65
60 12
NcoI C'CATG_G
Cuts at: 0 221 980 3582
Size: 221 759 2602
Fragments arranged by size:
2602 759 221
NsiI A_TGCA'T
Cuts at: 0 907 3582
Size: 907 2675
NspI r_CATG'y
Cuts at: 0 2278 2790 3582
Size: 2278 512 792
Fragments arranged by size:
2278 792 512
PasI CC'CwG_GG
Cuts at: 0 2466 3582
Size: 2466 1116
PflMI CCAn_nnn'nTGG
Cuts at: 0 1758 1782 1935 2550 2721 3582
Size: 1758 24 153 615 171 861
Fragments arranged by size:
1758 861 615 171 153 24
PfoI T'CCnGG_A
Cuts at: 0 755 908 1651 3582
Size: 755 153 743 1931
Fragments arranged by size:
1931 755 743 153
PmlI CAC'GTG
Cuts at: 0 2349 3582
Size: 2349 1233
PpuMI rG'GwC_Cy
Cuts at: 0 82 145 480 985 1278 1725 1826 1991
Size: 82 63 335 505 293 447 101 165
Cuts at: 1991 2060 2741 3582
Size: 69 681 841
Fragments arranged by size:
841 681 505 447 335 293 165 101
82 69 63
PspOMI G'GGCC_C
Cuts at: 0 3108 3582
Size: 3108 474
PstI C_TGCA'G
Cuts at: 0 440 710 1297 2024 2042 2861 3206 3582
Size: 440 270 587 727 18 819 345 376
Fragments arranged by size:
819 727 587 440 376 345 270 18
PvuII CAG'CTG
Cuts at: 0 2505 2750 2913 3582
Size: 2505 245 163 669
Fragments arranged by size:
2505 669 245 163
SacI G_AGCT'C
Cuts at: 0 494 3582
Size: 494 3088
SacII CC_GC'GG
Cuts at: 0 267 3582
Size: 267 3315
SanDI GG'GwC_CC
Cuts at: 0 1725 3582
Size: 1725 1857
SbfI CC_TGCA'GG
Cuts at: 0 2861 3582
Size: 2861 721
SfcI C'TryA_G
Cuts at: 0 436 706 1293 1671 2020 2038 2745 2857
Size: 436 270 587 378 349 18 707 112
Cuts at: 2857 2919 3202 3582
Size: 62 283 380
Fragments arranged by size:
707 587 436 380 378 349 283 270
112 62 18
SmaI CCC'GGG
Cuts at: 0 337 1798 3582
Size: 337 1461 1784
Fragments arranged by size:
1784 1461 337
SmlI C'TyrA_G
Cuts at: 0 1045 1219 1267 2607 3582
Size: 1045 174 48 1340 975
Fragments arranged by size:
1340 1045 975 174 48
StyI C'CwwG_G
Cuts at: 0 221 980 3142 3582
Size: 221 759 2162 440
Fragments arranged by size:
2162 759 440 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 31 268 381 1536 2215 3582
Size: 31 237 113 1155 679 1367
Fragments arranged by size:
1367 1155 679 237 113 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 384 416 757 789 969 1001 1552 1584
Size: 384 32 341 32 180 32 551 32
Cuts at: 1584 3582
Size: 1998
Fragments arranged by size:
1998 551 384 341 180 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1069 3582
Size: 1069 2513
XbaI T'CTAG_A
Cuts at: 0 3324 3582
Size: 3324 258
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 763 3582
Size: 763 2819
XmaI C'CCGG_G
Cuts at: 0 335 1796 3582
Size: 335 1461 1786
Fragments arranged by size:
1786 1461 335
XmnI GAAnn'nnTTC
Cuts at: 0 277 795 3582
Size: 277 518 2787
Fragments arranged by size:
2787 518 277
Enzymes that do cut:
AarI Acc65I AcuI AflII AleI AlfI AlwNI ApaI
ApoI AvaI BaeI BamHI BanI BanII BbsI BciVI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII HindIII KpnI MmeI MscI MslI MspA1I
NcoI NsiI NspI PasI PflMI PfoI PmlI PpuMI
PspOMI PstI PvuII SacI SacII SanDI SbfI SfcI
SmaI SmlI StyI TaqII TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflIII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvrII BbeI BbvCI BcgI
BclI BmgBI BmtI BsaBI BsiWI BspHI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I ClaI DraI EagI EcoRV
FseI FspI FspAI HpaI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PciI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
XhoI ZraI