(Linear) (Six-base) MAPSORT of: KIAA0475.seq Check: 1906 from: 1 to: 1227 KIAA0475.orf With 182 enzymes: SgfI * October 24, 2006 15:12 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 254 1227 Size: 254 973 ApaLI G'TGCA_C Cuts at: 0 1164 1227 Size: 1164 63 AvaI C'yCGr_G Cuts at: 0 218 1227 Size: 218 1009 BanI G'GyrC_C Cuts at: 0 156 1227 Size: 156 1071 BanII G_rGCy'C Cuts at: 0 486 1227 Size: 486 741 BbsI GAAGACnn'nnnn_ Cuts at: 0 1208 1227 Size: 1208 19 BfrBI ATG'CAT Cuts at: 0 264 1227 Size: 264 963 Bme1580I G_kGCm'C Cuts at: 0 1168 1227 Size: 1168 59 BmrI ACTGGGnnnn_n' Cuts at: 0 198 1087 1227 Size: 198 889 140 Fragments arranged by size: 889 198 140 Bpu10I CC'TnA_GC Cuts at: 0 371 1227 Size: 371 856 BsaI GGTCTCn'nnnn_ Cuts at: 0 380 1227 Size: 380 847 BsaBI GATnn'nnATC Cuts at: 0 1109 1227 Size: 1109 118 BsaHI Gr'CG_yC Cuts at: 0 1118 1227 Size: 1118 109 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1072 1102 1227 Size: 1072 30 125 Fragments arranged by size: 1072 125 30 BseYI C'CCAG_C Cuts at: 0 249 1227 Size: 249 978 BsiHKAI G_wGCw'C Cuts at: 0 559 1100 1168 1227 Size: 559 541 68 59 Fragments arranged by size: 559 541 68 59 BsmI GAATG_Cn' Cuts at: 0 968 1227 Size: 968 259 Bsp1286I G_dGCh'C Cuts at: 0 486 559 1100 1168 1227 Size: 486 73 541 68 59 Fragments arranged by size: 541 486 73 68 59 BsrDI GCAATG_nn' Cuts at: 0 864 1227 Size: 864 363 BstXI CCAn_nnnn'nTGG Cuts at: 0 683 1227 Size: 683 544 BstYI r'GATC_y Cuts at: 0 655 1227 Size: 655 572 BtgI C'CryG_G Cuts at: 0 269 709 1070 1121 1227 Size: 269 440 361 51 106 Fragments arranged by size: 440 361 269 106 51 BtsI GCAGTG_nn' Cuts at: 0 1168 1227 Size: 1168 59 DrdI GACnn_nn'nnGTC Cuts at: 0 835 1227 Size: 835 392 EaeI y'GGCC_r Cuts at: 0 272 740 1148 1227 Size: 272 468 408 79 Fragments arranged by size: 468 408 272 79 EarI CTCTTCn'nnn_ Cuts at: 0 230 1227 Size: 230 997 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 254 1227 Size: 254 973 EcoO109I rG'GnC_Cy Cuts at: 0 116 1227 Size: 116 1111 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1074 1227 Size: 1074 153 HaeII r_GCGC'y Cuts at: 0 905 1227 Size: 905 322 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1072 1104 1227 Size: 1072 32 123 Fragments arranged by size: 1072 123 32 HindIII A'AGCT_T Cuts at: 0 936 1227 Size: 936 291 MscI TGG'CCA Cuts at: 0 274 742 1150 1227 Size: 274 468 408 77 Fragments arranged by size: 468 408 274 77 MslI CAynn'nnrTG Cuts at: 0 875 1003 1227 Size: 875 128 224 Fragments arranged by size: 875 224 128 MspA1I CmG'CkG Cuts at: 0 94 321 549 835 1131 1227 Size: 94 227 228 286 296 96 Fragments arranged by size: 296 286 228 227 96 94 NcoI C'CATG_G Cuts at: 0 269 709 1070 1227 Size: 269 440 361 157 Fragments arranged by size: 440 361 269 157 NsiI A_TGCA'T Cuts at: 0 266 1227 Size: 266 961 NspI r_CATG'y Cuts at: 0 268 1227 Size: 268 959 PasI CC'CwG_GG Cuts at: 0 188 1227 Size: 188 1039 PflMI CCAn_nnn'nTGG Cuts at: 0 210 396 1227 Size: 210 186 831 Fragments arranged by size: 831 210 186 PsiI TTA'TAA Cuts at: 0 308 1227 Size: 308 919 PstI C_TGCA'G Cuts at: 0 695 1227 Size: 695 532 PvuII CAG'CTG Cuts at: 0 94 321 549 835 1227 Size: 94 227 228 286 392 Fragments arranged by size: 392 286 228 227 94 ScaI AGT'ACT Cuts at: 0 1193 1227 Size: 1193 34 SfcI C'TryA_G Cuts at: 0 569 691 1227 Size: 569 122 536 Fragments arranged by size: 569 536 122 SmaI CCC'GGG Cuts at: 0 220 1227 Size: 220 1007 SphI G_CATG'C Cuts at: 0 268 1227 Size: 268 959 StyI C'CwwG_G Cuts at: 0 269 488 709 1070 1227 Size: 269 219 221 361 157 Fragments arranged by size: 361 269 221 219 157 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 174 198 722 1227 Size: 174 24 524 505 Fragments arranged by size: 524 505 174 24 TatI w'GTAC_w Cuts at: 0 1191 1227 Size: 1191 36 XmaI C'CCGG_G Cuts at: 0 218 1227 Size: 218 1009 Enzymes that do cut: AcuI ApaLI AvaI BanI BanII BbsI BfrBI Bme1580I BmrI Bpu10I BsaI BsaBI BsaHI BsaXI BseYI BsiHKAI BsmI Bsp1286I BsrDI BstXI BstYI BtgI BtsI DrdI EaeI EarI Eco57MI EcoO109I FalI HaeII Hin4I HindIII MscI MslI MspA1I NcoI NsiI NspI PasI PflMI PsiI PstI PvuII ScaI SfcI SmaI SphI StyI TaqII TatI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI BmgBI BmtI BplI BpmI BpuEI BsaAI BsaWI BseRI BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmnI ZraI