Restriction map for pF1KSDA0475
 (Linear) (Six-base) MAPSORT of: KIAA0475.seq  Check: 1906  from: 1  to: 1227



KIAA0475.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254    1227 
   Size:        254     973
 
ApaLI G'TGCA_C

Cuts at:      0    1164    1227 
   Size:       1164      63
 
AvaI C'yCGr_G

Cuts at:      0     218    1227 
   Size:        218    1009
 
BanI G'GyrC_C

Cuts at:      0     156    1227 
   Size:        156    1071
 
BanII G_rGCy'C

Cuts at:      0     486    1227 
   Size:        486     741
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1208    1227 
   Size:       1208      19
 
BfrBI ATG'CAT

Cuts at:      0     264    1227 
   Size:        264     963
 
Bme1580I G_kGCm'C

Cuts at:      0    1168    1227 
   Size:       1168      59
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     198    1087    1227 
   Size:        198     889     140

  Fragments arranged by size: 

                 889     198     140
 
Bpu10I CC'TnA_GC

Cuts at:      0     371    1227 
   Size:        371     856
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     380    1227 
   Size:        380     847
 
BsaBI GATnn'nnATC

Cuts at:      0    1109    1227 
   Size:       1109     118
 
BsaHI Gr'CG_yC

Cuts at:      0    1118    1227 
   Size:       1118     109
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1072    1102    1227 
   Size:       1072      30     125

  Fragments arranged by size: 

                1072     125      30
 
BseYI C'CCAG_C

Cuts at:      0     249    1227 
   Size:        249     978
 
BsiHKAI G_wGCw'C

Cuts at:      0     559    1100    1168    1227 
   Size:        559     541      68      59

  Fragments arranged by size: 

                 559     541      68      59
 
BsmI GAATG_Cn'

Cuts at:      0     968    1227 
   Size:        968     259
 
Bsp1286I G_dGCh'C

Cuts at:      0     486     559    1100    1168    1227 
   Size:        486      73     541      68      59

  Fragments arranged by size: 

                 541     486      73      68      59
 
BsrDI GCAATG_nn'

Cuts at:      0     864    1227 
   Size:        864     363
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     683    1227 
   Size:        683     544
 
BstYI r'GATC_y

Cuts at:      0     655    1227 
   Size:        655     572
 
BtgI C'CryG_G

Cuts at:      0     269     709    1070    1121    1227 
   Size:        269     440     361      51     106

  Fragments arranged by size: 

                 440     361     269     106      51
 
BtsI GCAGTG_nn'

Cuts at:      0    1168    1227 
   Size:       1168      59
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     835    1227 
   Size:        835     392
 
EaeI y'GGCC_r

Cuts at:      0     272     740    1148    1227 
   Size:        272     468     408      79

  Fragments arranged by size: 

                 468     408     272      79
 
EarI CTCTTCn'nnn_

Cuts at:      0     230    1227 
   Size:        230     997
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254    1227 
   Size:        254     973
 
EcoO109I rG'GnC_Cy

Cuts at:      0     116    1227 
   Size:        116    1111
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1074    1227 
   Size:       1074     153
 
HaeII r_GCGC'y

Cuts at:      0     905    1227 
   Size:        905     322
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1072    1104    1227 
   Size:       1072      32     123

  Fragments arranged by size: 

                1072     123      32
 
HindIII A'AGCT_T

Cuts at:      0     936    1227 
   Size:        936     291
 
MscI TGG'CCA

Cuts at:      0     274     742    1150    1227 
   Size:        274     468     408      77

  Fragments arranged by size: 

                 468     408     274      77
 
MslI CAynn'nnrTG

Cuts at:      0     875    1003    1227 
   Size:        875     128     224

  Fragments arranged by size: 

                 875     224     128
 
MspA1I CmG'CkG

Cuts at:      0      94     321     549     835    1131    1227 
   Size:         94     227     228     286     296      96

  Fragments arranged by size: 

                 296     286     228     227      96      94
 
NcoI C'CATG_G

Cuts at:      0     269     709    1070    1227 
   Size:        269     440     361     157

  Fragments arranged by size: 

                 440     361     269     157
 
NsiI A_TGCA'T

Cuts at:      0     266    1227 
   Size:        266     961
 
NspI r_CATG'y

Cuts at:      0     268    1227 
   Size:        268     959
 
PasI CC'CwG_GG

Cuts at:      0     188    1227 
   Size:        188    1039
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     210     396    1227 
   Size:        210     186     831

  Fragments arranged by size: 

                 831     210     186
 
PsiI TTA'TAA

Cuts at:      0     308    1227 
   Size:        308     919
 
PstI C_TGCA'G

Cuts at:      0     695    1227 
   Size:        695     532
 
PvuII CAG'CTG

Cuts at:      0      94     321     549     835    1227 
   Size:         94     227     228     286     392

  Fragments arranged by size: 

                 392     286     228     227      94
 
ScaI AGT'ACT

Cuts at:      0    1193    1227 
   Size:       1193      34
 
SfcI C'TryA_G

Cuts at:      0     569     691    1227 
   Size:        569     122     536

  Fragments arranged by size: 

                 569     536     122
 
SmaI CCC'GGG

Cuts at:      0     220    1227 
   Size:        220    1007
 
SphI G_CATG'C

Cuts at:      0     268    1227 
   Size:        268     959
 
StyI C'CwwG_G

Cuts at:      0     269     488     709    1070    1227 
   Size:        269     219     221     361     157

  Fragments arranged by size: 

                 361     269     221     219     157
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     174     198     722    1227 
   Size:        174      24     524     505

  Fragments arranged by size: 

                 524     505     174      24
 
TatI w'GTAC_w

Cuts at:      0    1191    1227 
   Size:       1191      36
 
XmaI C'CCGG_G

Cuts at:      0     218    1227 
   Size:        218    1009
 

 Enzymes that do cut:

     AcuI    ApaLI     AvaI     BanI    BanII     BbsI    BfrBI Bme1580I
     BmrI   Bpu10I     BsaI    BsaBI    BsaHI    BsaXI    BseYI  BsiHKAI
     BsmI Bsp1286I    BsrDI    BstXI    BstYI     BtgI     BtsI     DrdI
     EaeI     EarI  Eco57MI EcoO109I     FalI    HaeII    Hin4I  HindIII
     MscI     MslI   MspA1I     NcoI     NsiI     NspI     PasI    PflMI
     PsiI     PstI    PvuII     ScaI     SfcI     SmaI     SphI     StyI
    TaqII     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI     BpmI    BpuEI    BsaAI    BsaWI    BseRI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI
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