Restriction map for
pF1KSDA0479
(Linear) (Six-base) MAPSORT of: KIAA0479.seq Check: 5454 from: 1 to: 921
KIAA0479.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 571 921
Size: 571 350
ApoI r'AATT_y
Cuts at: 0 225 921
Size: 225 696
AvaI C'yCGr_G
Cuts at: 0 692 921
Size: 692 229
BamHI G'GATC_C
Cuts at: 0 581 921
Size: 581 340
BanI G'GyrC_C
Cuts at: 0 191 687 921
Size: 191 496 234
Fragments arranged by size:
496 234 191
BanII G_rGCy'C
Cuts at: 0 635 921
Size: 635 286
BbvCI CC'TCA_GC
Cuts at: 0 478 921
Size: 478 443
BglII A'GATC_T
Cuts at: 0 452 921
Size: 452 469
Bme1580I G_kGCm'C
Cuts at: 0 71 550 692 921
Size: 71 479 142 229
Fragments arranged by size:
479 229 142 71
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 493 921
Size: 493 428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 921
Size: 631 290
Bpu10I CC'TnA_GC
Cuts at: 0 478 921
Size: 478 443
BsaI GGTCTCn'nnnn_
Cuts at: 0 1 388 921
Size: 1 387 533
Fragments arranged by size:
533 387 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 720 921
Size: 720 201
BseYI C'CCAG_C
Cuts at: 0 401 866 921
Size: 401 465 55
Fragments arranged by size:
465 401 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 921
Size: 88 833
BsmI GAATG_Cn'
Cuts at: 0 540 921
Size: 540 381
BsmBI CGTCTCn'nnnn_
Cuts at: 0 202 921
Size: 202 719
Bsp1286I G_dGCh'C
Cuts at: 0 71 550 635 692 921
Size: 71 479 85 57 229
Fragments arranged by size:
479 229 85 71 57
BspHI T'CATG_A
Cuts at: 0 314 719 921
Size: 314 405 202
Fragments arranged by size:
405 314 202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 292 614 921
Size: 292 322 307
Fragments arranged by size:
322 307 292
BsrFI r'CCGG_y
Cuts at: 0 188 504 870 921
Size: 188 316 366 51
Fragments arranged by size:
366 316 188 51
BsrGI T'GTAC_A
Cuts at: 0 902 921
Size: 902 19
BssSI C'ACGA_G
Cuts at: 0 178 921
Size: 178 743
BstXI CCAn_nnnn'nTGG
Cuts at: 0 456 635 921
Size: 456 179 286
Fragments arranged by size:
456 286 179
BstYI r'GATC_y
Cuts at: 0 452 485 581 921
Size: 452 33 96 340
Fragments arranged by size:
452 340 96 33
BtsI GCAGTG_nn'
Cuts at: 0 440 921
Size: 440 481
EaeI y'GGCC_r
Cuts at: 0 215 428 843 921
Size: 215 213 415 78
Fragments arranged by size:
415 215 213 78
EarI CTCTTCn'nnn_
Cuts at: 0 313 560 921
Size: 313 247 361
Fragments arranged by size:
361 313 247
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 487 921
Size: 487 434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 921
Size: 631 290
EcoO109I rG'GnC_Cy
Cuts at: 0 174 309 921
Size: 174 135 612
Fragments arranged by size:
612 174 135
EcoRI G'AATT_C
Cuts at: 0 225 921
Size: 225 696
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 847 879 921
Size: 847 32 42
Fragments arranged by size:
847 42 32
HincII GTy'rAC
Cuts at: 0 351 921
Size: 351 570
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 921
Size: 275 646
MscI TGG'CCA
Cuts at: 0 430 921
Size: 430 491
MslI CAynn'nnrTG
Cuts at: 0 76 553 763 766 921
Size: 76 477 210 3 155
Fragments arranged by size:
477 210 155 76 3
MspA1I CmG'CkG
Cuts at: 0 213 900 921
Size: 213 687 21
Fragments arranged by size:
687 213 21
NaeI GCC'GGC
Cuts at: 0 190 921
Size: 190 731
NgoMIV G'CCGG_C
Cuts at: 0 188 921
Size: 188 733
PasI CC'CwG_GG
Cuts at: 0 628 921
Size: 628 293
PflMI CCAn_nnn'nTGG
Cuts at: 0 852 921
Size: 852 69
PpuMI rG'GwC_Cy
Cuts at: 0 309 921
Size: 309 612
PstI C_TGCA'G
Cuts at: 0 289 447 833 921
Size: 289 158 386 88
Fragments arranged by size:
386 289 158 88
PvuII CAG'CTG
Cuts at: 0 213 900 921
Size: 213 687 21
Fragments arranged by size:
687 213 21
SfcI C'TryA_G
Cuts at: 0 285 443 829 921
Size: 285 158 386 92
Fragments arranged by size:
386 285 158 92
SgrAI Cr'CCGG_yG
Cuts at: 0 504 921
Size: 504 417
SmaI CCC'GGG
Cuts at: 0 694 921
Size: 694 227
StyI C'CwwG_G
Cuts at: 0 250 921
Size: 250 671
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 728 921
Size: 19 709 193
Fragments arranged by size:
709 193 19
TatI w'GTAC_w
Cuts at: 0 902 921
Size: 902 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 477 509 921
Size: 477 32 412
Fragments arranged by size:
477 412 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 160 819 921
Size: 160 659 102
Fragments arranged by size:
659 160 102
XmaI C'CCGG_G
Cuts at: 0 692 921
Size: 692 229
Enzymes that do cut:
AlfI ApoI AvaI BamHI BanI BanII BbvCI BglII
Bme1580I BplI BpmI Bpu10I BsaI BseRI BseYI BsgI
BsmI BsmBI Bsp1286I BspHI BspMI BsrFI BsrGI BssSI
BstXI BstYI BtsI EaeI EarI EciI Eco57MI EcoO109I
EcoRI Hin4I HincII MmeI MscI MslI MspA1I NaeI
NgoMIV PasI PflMI PpuMI PstI PvuII SfcI SgrAI
SmaI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BbsI BcgI
BciVI BclI BfrBI BglI BlpI BmgBI BmrI BmtI
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI
BsiWI BspEI BsrBI BsrDI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmnI ZraI