(Linear) (Six-base) MAPSORT of: KIAA0487.seq Check: 5834 from: 1 to: 540
KIAA0487.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
Acc65I G'GTAC_C
Cuts at: 0 122 540
Size: 122 418
AlwNI CAG_nnn'CTG
Cuts at: 0 301 540
Size: 301 239
ApoI r'AATT_y
Cuts at: 0 456 540
Size: 456 84
AvaI C'yCGr_G
Cuts at: 0 163 540
Size: 163 377
BanI G'GyrC_C
Cuts at: 0 122 540
Size: 122 418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 370 540
Size: 233 137 170
Fragments arranged by size:
233 170 137
BsaWI w'CCGG_w
Cuts at: 0 77 477 540
Size: 77 400 63
Fragments arranged by size:
400 77 63
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 164 181 220 540
Size: 164 17 39 320
Fragments arranged by size:
320 164 39 17
BseYI C'CCAG_C
Cuts at: 0 432 444 540
Size: 432 12 96
Fragments arranged by size:
432 96 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 540
Size: 491 49
BsiEI CG_ry'CG
Cuts at: 0 39 540
Size: 39 501
BspEI T'CCGG_A
Cuts at: 0 77 477 540
Size: 77 400 63
Fragments arranged by size:
400 77 63
BsrFI r'CCGG_y
Cuts at: 0 119 540
Size: 119 421
BsrGI T'GTAC_A
Cuts at: 0 402 540
Size: 402 138
BstXI CCAn_nnnn'nTGG
Cuts at: 0 134 540
Size: 134 406
EarI CTCTTCn'nnn_
Cuts at: 0 340 540
Size: 340 200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 370 540
Size: 233 137 170
Fragments arranged by size:
233 170 137
KpnI G_GTAC'C
Cuts at: 0 126 540
Size: 126 414
MspA1I CmG'CkG
Cuts at: 0 505 540
Size: 505 35
NdeI CA'TA_TG
Cuts at: 0 279 540
Size: 279 261
NspI r_CATG'y
Cuts at: 0 409 540
Size: 409 131
PflMI CCAn_nnn'nTGG
Cuts at: 0 451 540
Size: 451 89
SfcI C'TryA_G
Cuts at: 0 461 540
Size: 461 79
SspI AAT'ATT
Cuts at: 0 308 336 540
Size: 308 28 204
Fragments arranged by size:
308 204 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 278 540
Size: 278 262
TatI w'GTAC_w
Cuts at: 0 402 540
Size: 402 138
Enzymes that do cut:
Acc65I AlwNI ApoI AvaI BanI BpmI BsaWI BseRI
BseYI BsgI BsiEI BspEI BsrFI BsrGI BstXI EarI
Eco57MI KpnI MspA1I NdeI NspI PflMI SfcI SspI
TaqII TatI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI MfeI
MluI MmeI MscI MslI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI