Restriction map for pF1KA0491
 (Linear) (Six-base) MAPSORT of: KIAA0491.seq  Check: 599  from: 1  to: 1095



KIAA0491.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209    1095 
   Size:        209     886
 
AflII C'TTAA_G

Cuts at:      0     901    1095 
   Size:        901     194
 
AlwNI CAG_nnn'CTG

Cuts at:      0     316    1095 
   Size:        316     779
 
ApoI r'AATT_y

Cuts at:      0     229     423     564     601    1095 
   Size:        229     194     141      37     494

  Fragments arranged by size: 

                 494     229     194     141      37
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     794     827    1095 
   Size:        794      33     268

  Fragments arranged by size: 

                 794     268      33
 
BanI G'GyrC_C

Cuts at:      0      46    1065    1095 
   Size:         46    1019      30

  Fragments arranged by size: 

                1019      46      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     109    1095 
   Size:        109     986
 
BclI T'GATC_A

Cuts at:      0     992    1095 
   Size:        992     103
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     763    1095 
   Size:        763     332
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     727    1095 
   Size:        727     368
 
Bpu10I CC'TnA_GC

Cuts at:      0     136    1095 
   Size:        136     959
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88    1095 
   Size:         88    1007
 
BsiEI CG_ry'CG

Cuts at:      0      38    1095 
   Size:         38    1057
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     550    1095 
   Size:        550     545
 
BtgI C'CryG_G

Cuts at:      0     848    1095 
   Size:        848     247
 
EaeI y'GGCC_r

Cuts at:      0      35      90    1095 
   Size:         35      55    1005

  Fragments arranged by size: 

                1005      55      35
 
EagI C'GGCC_G

Cuts at:      0      35    1095 
   Size:         35    1060
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     727    1095 
   Size:        209     518     368

  Fragments arranged by size: 

                 518     368     209
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     103    1095 
   Size:        103     992
 
HindIII A'AGCT_T

Cuts at:      0      85    1095 
   Size:         85    1010
 
MfeI C'AATT_G

Cuts at:      0     470    1095 
   Size:        470     625
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     716     911     993    1095 
   Size:        716     195      82     102

  Fragments arranged by size: 

                 716     195     102      82
 
MscI TGG'CCA

Cuts at:      0      92    1095 
   Size:         92    1003
 
NcoI C'CATG_G

Cuts at:      0     848    1095 
   Size:        848     247
 
NspI r_CATG'y

Cuts at:      0     661    1095 
   Size:        661     434
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     161    1095 
   Size:        161     934
 
PstI C_TGCA'G

Cuts at:      0     555    1095 
   Size:        555     540
 
ScaI AGT'ACT

Cuts at:      0     963    1095 
   Size:        963     132
 
SfcI C'TryA_G

Cuts at:      0     551    1095 
   Size:        551     544
 
SmlI C'TyrA_G

Cuts at:      0     901    1095 
   Size:        901     194
 
StyI C'CwwG_G

Cuts at:      0     848    1095 
   Size:        848     247
 
TatI w'GTAC_w

Cuts at:      0     652     961    1095 
   Size:        652     309     134

  Fragments arranged by size: 

                 652     309     134
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     379    1095 
   Size:        379     716
 
XmnI GAAnn'nnTTC

Cuts at:      0     404    1020    1095 
   Size:        404     616      75

  Fragments arranged by size: 

                 616     404      75
 

 Enzymes that do cut:

     AcuI    AflII    AlwNI     ApoI     BaeI     BanI     BbsI     BclI
     BmrI     BpmI   Bpu10I     BsgI    BsiEI   BstAPI     BtgI     EaeI
     EagI  Eco57MI     FalI  HindIII     MfeI     MmeI     MscI     NcoI
     NspI    PflMI     PstI     ScaI     SfcI     SmlI     StyI     TatI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII    BamHI    BanII     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MluI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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