Restriction map for
pF1KA0512
(Linear) (Six-base) MAPSORT of: KIAA0512.seq Check: 3947 from: 1 to: 1896
KIAA0512.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1520 1896
Size: 1520 376
AccI GT'mk_AC
Cuts at: 0 71 911 1896
Size: 71 840 985
Fragments arranged by size:
985 840 71
Acc65I G'GTAC_C
Cuts at: 0 831 1896
Size: 831 1065
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 638 1168 1378 1896
Size: 251 387 530 210 518
Fragments arranged by size:
530 518 387 251 210
AfeI AGC'GCT
Cuts at: 0 312 1896
Size: 312 1584
AhdI GACnn_n'nnGTC
Cuts at: 0 195 584 1044 1896
Size: 195 389 460 852
Fragments arranged by size:
852 460 389 195
AleI CACnn'nnGTG
Cuts at: 0 574 1896
Size: 574 1322
AlwNI CAG_nnn'CTG
Cuts at: 0 823 958 961 970 1060 1896
Size: 823 135 3 9 90 836
Fragments arranged by size:
836 823 135 90 9 3
ApaI G_GGCC'C
Cuts at: 0 249 1896
Size: 249 1647
ApoI r'AATT_y
Cuts at: 0 1471 1599 1768 1891 1896
Size: 1471 128 169 123 5
Fragments arranged by size:
1471 169 128 123 5
AseI AT'TA_AT
Cuts at: 0 1703 1896
Size: 1703 193
AvaI C'yCGr_G
Cuts at: 0 427 625 1066 1896
Size: 427 198 441 830
Fragments arranged by size:
830 441 427 198
BanI G'GyrC_C
Cuts at: 0 55 528 546 831 1896
Size: 55 473 18 285 1065
Fragments arranged by size:
1065 473 285 55 18
BanII G_rGCy'C
Cuts at: 0 249 480 789 1067 1896
Size: 249 231 309 278 829
Fragments arranged by size:
829 309 278 249 231
BbsI GAAGACnn'nnnn_
Cuts at: 0 1101 1896
Size: 1101 795
BbvCI CC'TCA_GC
Cuts at: 0 284 314 653 1896
Size: 284 30 339 1243
Fragments arranged by size:
1243 339 284 30
BclI T'GATC_A
Cuts at: 0 1307 1896
Size: 1307 589
BglI GCCn_nnn'nGGC
Cuts at: 0 349 448 1348 1896
Size: 349 99 900 548
Fragments arranged by size:
900 548 349 99
Bme1580I G_kGCm'C
Cuts at: 0 249 668 1896
Size: 249 419 1228
Fragments arranged by size:
1228 419 249
BmrI ACTGGGnnnn_n'
Cuts at: 0 214 689 707 803 928 1896
Size: 214 475 18 96 125 968
Fragments arranged by size:
968 475 214 125 96 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 845 959 1829 1896
Size: 167 678 114 870 67
Fragments arranged by size:
870 678 167 114 67
Bpu10I CC'TnA_GC
Cuts at: 0 284 314 653 1836 1896
Size: 284 30 339 1183 60
Fragments arranged by size:
1183 339 284 60 30
BsaI GGTCTCn'nnnn_
Cuts at: 0 86 1063 1896
Size: 86 977 833
Fragments arranged by size:
977 833 86
BsaAI yAC'GTr
Cuts at: 0 1683 1896
Size: 1683 213
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 350 380 1015 1045 1802 1832 1896
Size: 350 30 635 30 757 30 64
Fragments arranged by size:
757 635 350 64 30 30 30
BseYI C'CCAG_C
Cuts at: 0 139 163 297 1073 1896
Size: 139 24 134 776 823
Fragments arranged by size:
823 776 139 134 24
Bsp1286I G_dGCh'C
Cuts at: 0 249 480 668 789 1067 1896
Size: 249 231 188 121 278 829
Fragments arranged by size:
829 278 249 231 188 121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1520 1896
Size: 1520 376
BsrBI CCG'CTC
Cuts at: 0 829 1896
Size: 829 1067
BsrDI GCAATG_nn'
Cuts at: 0 407 473 1526 1896
Size: 407 66 1053 370
Fragments arranged by size:
1053 407 370 66
BsrFI r'CCGG_y
Cuts at: 0 1388 1896
Size: 1388 508
BsrGI T'GTAC_A
Cuts at: 0 1400 1896
Size: 1400 496
BstAPI GCAn_nnn'nTGC
Cuts at: 0 958 961 970 1896
Size: 958 3 9 926
Fragments arranged by size:
958 926 9 3
BstBI TT'CG_AA
Cuts at: 0 1597 1896
Size: 1597 299
BstEII G'GTnAC_C
Cuts at: 0 576 1896
Size: 576 1320
Bsu36I CC'TnA_GG
Cuts at: 0 374 1896
Size: 374 1522
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 620 1896
Size: 620 1276
BtsI GCAGTG_nn'
Cuts at: 0 296 1896
Size: 296 1600
EaeI y'GGCC_r
Cuts at: 0 113 1349 1896
Size: 113 1236 547
Fragments arranged by size:
1236 547 113
EarI CTCTTCn'nnn_
Cuts at: 0 100 1896
Size: 100 1796
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 995 1896
Size: 995 901
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 251 638 845 959 1168 1378 1829
Size: 167 84 387 207 114 209 210 451
Cuts at: 1829 1896
Size: 67
Fragments arranged by size:
451 387 210 209 207 167 114 84
67
EcoNI CCTnn'n_nnAGG
Cuts at: 0 997 1896
Size: 997 899
EcoO109I rG'GnC_Cy
Cuts at: 0 245 441 586 863 1002 1170 1896
Size: 245 196 145 277 139 168 726
Fragments arranged by size:
726 277 245 196 168 145 139
EcoRI G'AATT_C
Cuts at: 0 1768 1896
Size: 1768 128
EcoRV GAT'ATC
Cuts at: 0 1425 1896
Size: 1425 471
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1137 1801 1896
Size: 1137 664 95
Fragments arranged by size:
1137 664 95
HaeII r_GCGC'y
Cuts at: 0 314 1119 1896
Size: 314 805 777
Fragments arranged by size:
805 777 314
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 170 181 202 213 244 276 570 602
Size: 170 11 21 11 31 32 294 32
Cuts at: 602 1030 1042 1062 1074 1896
Size: 428 12 20 12 822
Fragments arranged by size:
822 428 294 170 32 32 31 21
20 12 12 11 11
KpnI G_GTAC'C
Cuts at: 0 835 1896
Size: 835 1061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 1233 1443 1896
Size: 258 975 210 453
Fragments arranged by size:
975 453 258 210
MscI TGG'CCA
Cuts at: 0 115 1351 1896
Size: 115 1236 545
Fragments arranged by size:
1236 545 115
MslI CAynn'nnrTG
Cuts at: 0 574 1896
Size: 574 1322
MspA1I CmG'CkG
Cuts at: 0 301 526 652 742 763 958 1896
Size: 301 225 126 90 21 195 938
Fragments arranged by size:
938 301 225 195 126 90 21
NaeI GCC'GGC
Cuts at: 0 1390 1896
Size: 1390 506
NgoMIV G'CCGG_C
Cuts at: 0 1388 1896
Size: 1388 508
PasI CC'CwG_GG
Cuts at: 0 781 1896
Size: 781 1115
PfoI T'CCnGG_A
Cuts at: 0 714 750 865 936 1896
Size: 714 36 115 71 960
Fragments arranged by size:
960 714 115 71 36
PpuMI rG'GwC_Cy
Cuts at: 0 586 863 1002 1170 1896
Size: 586 277 139 168 726
Fragments arranged by size:
726 586 277 168 139
PsiI TTA'TAA
Cuts at: 0 1876 1896
Size: 1876 20
PspOMI G'GGCC_C
Cuts at: 0 245 1896
Size: 245 1651
PstI C_TGCA'G
Cuts at: 0 456 690 708 954 966 1896
Size: 456 234 18 246 12 930
Fragments arranged by size:
930 456 246 234 18 12
PvuII CAG'CTG
Cuts at: 0 301 652 763 958 1896
Size: 301 351 111 195 938
Fragments arranged by size:
938 351 301 195 111
SexAI A'CCwGG_T
Cuts at: 0 859 1896
Size: 859 1037
SfcI C'TryA_G
Cuts at: 0 452 686 704 950 962 1761 1896
Size: 452 234 18 246 12 799 135
Fragments arranged by size:
799 452 246 234 135 18 12
SmaI CCC'GGG
Cuts at: 0 429 627 1896
Size: 429 198 1269
Fragments arranged by size:
1269 429 198
SpeI A'CTAG_T
Cuts at: 0 1881 1896
Size: 1881 15
StuI AGG'CCT
Cuts at: 0 1286 1896
Size: 1286 610
StyI C'CwwG_G
Cuts at: 0 192 444 608 1896
Size: 192 252 164 1288
Fragments arranged by size:
1288 252 192 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 582 595 1138 1896
Size: 582 13 543 758
Fragments arranged by size:
758 582 543 13
TatI w'GTAC_w
Cuts at: 0 1400 1896
Size: 1400 496
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1015 1047 1896
Size: 1015 32 849
Fragments arranged by size:
1015 849 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 108 352 1896
Size: 108 244 1544
Fragments arranged by size:
1544 244 108
XmaI C'CCGG_G
Cuts at: 0 427 625 1896
Size: 427 198 1271
Fragments arranged by size:
1271 427 198
XmnI GAAnn'nnTTC
Cuts at: 0 1891 1896
Size: 1891 5
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AhdI AleI AlwNI
ApaI ApoI AseI AvaI BanI BanII BbsI BbvCI
BclI BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI
BsaXI BseYI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstBI BstEII Bsu36I BtgZI BtsI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I KpnI MmeI MscI MslI MspA1I NaeI NgoMIV
PasI PfoI PpuMI PsiI PspOMI PstI PvuII SexAI
SfcI SmaI SpeI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AlfI AloI ApaLI
AscI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI
BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaBI
BsaHI BsaWI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BssHII BssSI BstXI BstYI BstZ17I
BtgI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
FseI FspI FspAI HincII HindIII HpaI KasI MfeI
MluI NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PshAI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI
SnaBI SphI SrfI SspI SwaI Tth111I XbaI XhoI
ZraI