Restriction map for
pF1KA0519
(Linear) (Six-base) MAPSORT of: KIAA0519.seq Check: 188 from: 1 to: 2757
KIAA0519.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1204 2757
Size: 1204 1553
AccI GT'mk_AC
Cuts at: 0 587 2757
Size: 587 2170
AclI AA'CG_TT
Cuts at: 0 674 2757
Size: 674 2083
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 1054 1168 1294 2757
Size: 355 699 114 126 1463
Fragments arranged by size:
1463 699 355 126 114
AfeI AGC'GCT
Cuts at: 0 1958 2757
Size: 1958 799
AleI CACnn'nnGTG
Cuts at: 0 1993 2757
Size: 1993 764
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1955 1987 2757
Size: 1955 32 770
Fragments arranged by size:
1955 770 32
AlwNI CAG_nnn'CTG
Cuts at: 0 381 1182 1900 2404 2595 2757
Size: 381 801 718 504 191 162
Fragments arranged by size:
801 718 504 381 191 162
ApoI r'AATT_y
Cuts at: 0 1189 1882 1945 2088 2757
Size: 1189 693 63 143 669
Fragments arranged by size:
1189 693 669 143 63
AvaI C'yCGr_G
Cuts at: 0 200 521 1306 1976 2291 2757
Size: 200 321 785 670 315 466
Fragments arranged by size:
785 670 466 321 315 200
BamHI G'GATC_C
Cuts at: 0 630 2757
Size: 630 2127
BanI G'GyrC_C
Cuts at: 0 1002 1372 1452 2757
Size: 1002 370 80 1305
Fragments arranged by size:
1305 1002 370 80
BanII G_rGCy'C
Cuts at: 0 278 434 977 1894 2757
Size: 278 156 543 917 863
Fragments arranged by size:
917 863 543 278 156
BbsI GAAGACnn'nnnn_
Cuts at: 0 114 2757
Size: 114 2643
BglI GCCn_nnn'nGGC
Cuts at: 0 795 892 2757
Size: 795 97 1865
Fragments arranged by size:
1865 795 97
BlpI GC'TnA_GC
Cuts at: 0 94 761 2757
Size: 94 667 1996
Fragments arranged by size:
1996 667 94
Bme1580I G_kGCm'C
Cuts at: 0 1239 2757
Size: 1239 1518
BmrI ACTGGGnnnn_n'
Cuts at: 0 891 2757
Size: 891 1866
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1098 2306 2757
Size: 1098 1208 451
Fragments arranged by size:
1208 1098 451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 370 787 1805 2757
Size: 307 63 417 1018 952
Fragments arranged by size:
1018 952 417 307 63
Bpu10I CC'TnA_GC
Cuts at: 0 1893 2757
Size: 1893 864
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 2757
Size: 376 2381
BsaAI yAC'GTr
Cuts at: 0 89 234 714 838 2757
Size: 89 145 480 124 1919
Fragments arranged by size:
1919 480 145 124 89
BsaHI Gr'CG_yC
Cuts at: 0 2135 2757
Size: 2135 622
BsaWI w'CCGG_w
Cuts at: 0 1130 2757
Size: 1130 1627
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 760 790 1955 1985 2757
Size: 760 30 1165 30 772
Fragments arranged by size:
1165 772 760 30 30
BseYI C'CCAG_C
Cuts at: 0 1216 1389 1620 1927 2757
Size: 1216 173 231 307 830
Fragments arranged by size:
1216 830 307 231 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1177 2757
Size: 1177 1580
BsiHKAI G_wGCw'C
Cuts at: 0 278 434 1821 2708 2757
Size: 278 156 1387 887 49
Fragments arranged by size:
1387 887 278 156 49
BsmI GAATG_Cn'
Cuts at: 0 417 2757
Size: 417 2340
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1729 2757
Size: 1729 1028
Bsp1286I G_dGCh'C
Cuts at: 0 278 434 977 1239 1821 1894 2708 2757
Size: 278 156 543 262 582 73 814 49
Fragments arranged by size:
814 582 543 278 262 156 73 49
BspHI T'CATG_A
Cuts at: 0 2601 2757
Size: 2601 156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 61 389 1204 1389 2757
Size: 61 328 815 185 1368
Fragments arranged by size:
1368 815 328 185 61
BsrBI CCG'CTC
Cuts at: 0 132 1082 1251 1627 2013 2625 2757
Size: 132 950 169 376 386 612 132
Fragments arranged by size:
950 612 386 376 169 132 132
BsrDI GCAATG_nn'
Cuts at: 0 1136 1974 2498 2757
Size: 1136 838 524 259
Fragments arranged by size:
1136 838 524 259
BsrFI r'CCGG_y
Cuts at: 0 496 530 741 1005 2757
Size: 496 34 211 264 1752
Fragments arranged by size:
1752 496 264 211 34
BsrGI T'GTAC_A
Cuts at: 0 2678 2757
Size: 2678 79
BssSI C'ACGA_G
Cuts at: 0 665 1463 2620 2757
Size: 665 798 1157 137
Fragments arranged by size:
1157 798 665 137
BstAPI GCAn_nnn'nTGC
Cuts at: 0 381 2757
Size: 381 2376
BstBI TT'CG_AA
Cuts at: 0 1088 1355 2757
Size: 1088 267 1402
Fragments arranged by size:
1402 1088 267
BstEII G'GTnAC_C
Cuts at: 0 2550 2757
Size: 2550 207
BstXI CCAn_nnnn'nTGG
Cuts at: 0 984 1887 2148 2757
Size: 984 903 261 609
Fragments arranged by size:
984 903 609 261
BstYI r'GATC_y
Cuts at: 0 240 630 1616 1655 2757
Size: 240 390 986 39 1102
Fragments arranged by size:
1102 986 390 240 39
Bsu36I CC'TnA_GG
Cuts at: 0 2588 2757
Size: 2588 169
BtgI C'CryG_G
Cuts at: 0 887 2387 2757
Size: 887 1500 370
Fragments arranged by size:
1500 887 370
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 120 684 2757
Size: 120 564 2073
Fragments arranged by size:
2073 564 120
BtsI GCAGTG_nn'
Cuts at: 0 1435 1562 2757
Size: 1435 127 1195
Fragments arranged by size:
1435 1195 127
DraIII CAC_nnn'GTG
Cuts at: 0 1243 2757
Size: 1243 1514
DrdI GACnn_nn'nnGTC
Cuts at: 0 610 2134 2757
Size: 610 1524 623
Fragments arranged by size:
1524 623 610
EaeI y'GGCC_r
Cuts at: 0 879 2316 2757
Size: 879 1437 441
Fragments arranged by size:
1437 879 441
EarI CTCTTCn'nnn_
Cuts at: 0 266 349 1030 1085 1357 1634 2713 2757
Size: 266 83 681 55 272 277 1079 44
Fragments arranged by size:
1079 681 277 272 266 83 55 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 70 813 2236 2757
Size: 70 743 1423 521
Fragments arranged by size:
1423 743 521 70
EcoICRI GAG'CTC
Cuts at: 0 276 432 2757
Size: 276 156 2325
Fragments arranged by size:
2325 276 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 355 370 787 1054 1168 1294 1805
Size: 307 48 15 417 267 114 126 511
Cuts at: 1805 2757
Size: 952
Fragments arranged by size:
952 511 417 307 267 126 114 48
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1072 2068 2757
Size: 1072 996 689
Fragments arranged by size:
1072 996 689
EcoO109I rG'GnC_Cy
Cuts at: 0 378 1179 1311 1713 1825 2112 2592 2757
Size: 378 801 132 402 112 287 480 165
Fragments arranged by size:
801 480 402 378 287 165 132 112
EcoRI G'AATT_C
Cuts at: 0 1189 2088 2757
Size: 1189 899 669
Fragments arranged by size:
1189 899 669
HaeII r_GCGC'y
Cuts at: 0 300 1960 2757
Size: 300 1660 797
Fragments arranged by size:
1660 797 300
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 180 212 1038 1070 1590 1622 2757
Size: 180 32 826 32 520 32 1135
Fragments arranged by size:
1135 826 520 180 32 32 32
HincII GTy'rAC
Cuts at: 0 2003 2757
Size: 2003 754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 495 2390 2757
Size: 95 400 1895 367
Fragments arranged by size:
1895 400 367 95
MslI CAynn'nnrTG
Cuts at: 0 815 1993 2146 2503 2548 2571 2757
Size: 815 1178 153 357 45 23 186
Fragments arranged by size:
1178 815 357 186 153 45 23
MspA1I CmG'CkG
Cuts at: 0 285 954 1651 1808 2757
Size: 285 669 697 157 949
Fragments arranged by size:
949 697 669 285 157
NaeI GCC'GGC
Cuts at: 0 498 532 2757
Size: 498 34 2225
Fragments arranged by size:
2225 498 34
NcoI C'CATG_G
Cuts at: 0 887 2387 2757
Size: 887 1500 370
Fragments arranged by size:
1500 887 370
NgoMIV G'CCGG_C
Cuts at: 0 496 530 2757
Size: 496 34 2227
Fragments arranged by size:
2227 496 34
NspI r_CATG'y
Cuts at: 0 60 1425 2670 2757
Size: 60 1365 1245 87
Fragments arranged by size:
1365 1245 87 60
PfoI T'CCnGG_A
Cuts at: 0 254 1634 2462 2757
Size: 254 1380 828 295
Fragments arranged by size:
1380 828 295 254
PmlI CAC'GTG
Cuts at: 0 89 234 2757
Size: 89 145 2523
Fragments arranged by size:
2523 145 89
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1955 1987 2757
Size: 1955 32 770
Fragments arranged by size:
1955 770 32
PpuMI rG'GwC_Cy
Cuts at: 0 378 1179 1311 1713 2112 2757
Size: 378 801 132 402 399 645
Fragments arranged by size:
801 645 402 399 378 132
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2671 2703 2757
Size: 2671 32 54
Fragments arranged by size:
2671 54 32
PstI C_TGCA'G
Cuts at: 0 284 1430 2757
Size: 284 1146 1327
Fragments arranged by size:
1327 1146 284
PvuII CAG'CTG
Cuts at: 0 285 1651 1808 2757
Size: 285 1366 157 949
Fragments arranged by size:
1366 949 285 157
SacI G_AGCT'C
Cuts at: 0 278 434 2757
Size: 278 156 2323
Fragments arranged by size:
2323 278 156
SanDI GG'GwC_CC
Cuts at: 0 1311 2757
Size: 1311 1446
SapI GCTCTTCn'nnn_
Cuts at: 0 266 349 1357 2713 2757
Size: 266 83 1008 1356 44
Fragments arranged by size:
1356 1008 266 83 44
ScaI AGT'ACT
Cuts at: 0 876 2757
Size: 876 1881
SfcI C'TryA_G
Cuts at: 0 280 1426 2757
Size: 280 1146 1331
Fragments arranged by size:
1331 1146 280
SmaI CCC'GGG
Cuts at: 0 202 1308 2757
Size: 202 1106 1449
Fragments arranged by size:
1449 1106 202
SmlI C'TyrA_G
Cuts at: 0 391 2757
Size: 391 2366
SpeI A'CTAG_T
Cuts at: 0 720 2757
Size: 720 2037
SphI G_CATG'C
Cuts at: 0 60 2757
Size: 60 2697
StyI C'CwwG_G
Cuts at: 0 635 887 2387 2757
Size: 635 252 1500 370
Fragments arranged by size:
1500 635 370 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 427 1325 1773 2757
Size: 427 898 448 984
Fragments arranged by size:
984 898 448 427
TatI w'GTAC_w
Cuts at: 0 874 920 2678 2757
Size: 874 46 1758 79
Fragments arranged by size:
1758 874 79 46
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 941 973 2104 2136 2757
Size: 941 32 1131 32 621
Fragments arranged by size:
1131 941 621 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 940 2757
Size: 940 1817
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 613 925 2466 2757
Size: 613 312 1541 291
Fragments arranged by size:
1541 613 312 291
XmaI C'CCGG_G
Cuts at: 0 200 1306 2757
Size: 200 1106 1451
Fragments arranged by size:
1451 1106 200
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AleI AloI AlwNI
ApoI AvaI BamHI BanI BanII BbsI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI
BsaWI BsaXI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI HaeII Hin4I HincII MmeI MslI MspA1I
NaeI NcoI NgoMIV NspI PfoI PmlI PpiI PpuMI
PsrI PstI PvuII SacI SanDI SapI ScaI SfcI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AflII AflIII AgeI AhdI AlfI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI
BcgI BciVI BclI BfrBI BglII BmgBI BmtI BsaI
BsaBI BseRI BsiEI BsiWI BspEI BssHII BstZ17I ClaI
CspCI DraI EagI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MscI NarI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PshAI PsiI PspOMI PspXI PvuI RsrII
SacII SalI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SrfI SspI StuI SwaI XbaI XhoI XmnI
ZraI