Restriction map for
pF1KA0521
(Linear) (Six-base) MAPSORT of: KIAA0521.seq Check: 1450 from: 1 to: 3045
KIAA0521.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1851 3045
Size: 1851 1194
AatII G_ACGT'C
Cuts at: 0 3035 3045
Size: 3035 10
AccI GT'mk_AC
Cuts at: 0 2053 3045
Size: 2053 992
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 649 970 988 1332 1951 2782 3045
Size: 391 258 321 18 344 619 831 263
Fragments arranged by size:
831 619 391 344 321 263 258 18
AfeI AGC'GCT
Cuts at: 0 2167 2734 3045
Size: 2167 567 311
Fragments arranged by size:
2167 567 311
AflIII A'CryG_T
Cuts at: 0 2056 3045
Size: 2056 989
AhdI GACnn_n'nnGTC
Cuts at: 0 138 310 951 3045
Size: 138 172 641 2094
Fragments arranged by size:
2094 641 172 138
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2030 3045
Size: 2030 1015
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 391 423 2023 2055 3045
Size: 391 32 1600 32 990
Fragments arranged by size:
1600 990 391 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1614 1944 2202 2772 2775 2878 3045
Size: 1614 330 258 570 3 103 167
Fragments arranged by size:
1614 570 330 258 167 103 3
ApaI G_GGCC'C
Cuts at: 0 390 1346 2494 3045
Size: 390 956 1148 551
Fragments arranged by size:
1148 956 551 390
ApaLI G'TGCA_C
Cuts at: 0 340 3045
Size: 340 2705
ApoI r'AATT_y
Cuts at: 0 112 679 3045
Size: 112 567 2366
Fragments arranged by size:
2366 567 112
AvaI C'yCGr_G
Cuts at: 0 1520 1778 2495 2545 2892 3045
Size: 1520 258 717 50 347 153
Fragments arranged by size:
1520 717 347 258 153 50
BamHI G'GATC_C
Cuts at: 0 1934 3045
Size: 1934 1111
BanI G'GyrC_C
Cuts at: 0 1872 3045
Size: 1872 1173
BanII G_rGCy'C
Cuts at: 0 43 390 980 1270 1346 1846 2494 2728
Size: 43 347 590 290 76 500 648 234
Cuts at: 2728 2746 2836 2899 3045
Size: 18 90 63 146
Fragments arranged by size:
648 590 500 347 290 234 146 90
76 63 43 18
BbeI G_GCGC'C
Cuts at: 0 1876 3045
Size: 1876 1169
BbsI GAAGACnn'nnnn_
Cuts at: 0 842 947 1001 1048 1511 1661 3035 3045
Size: 842 105 54 47 463 150 1374 10
Fragments arranged by size:
1374 842 463 150 105 54 47 10
BbvCI CC'TCA_GC
Cuts at: 0 199 3045
Size: 199 2846
BclI T'GATC_A
Cuts at: 0 692 1220 3045
Size: 692 528 1825
Fragments arranged by size:
1825 692 528
BglI GCCn_nnn'nGGC
Cuts at: 0 47 1294 3045
Size: 47 1247 1751
Fragments arranged by size:
1751 1247 47
BglII A'GATC_T
Cuts at: 0 1469 3045
Size: 1469 1576
BlpI GC'TnA_GC
Cuts at: 0 2617 2745 2830 3045
Size: 2617 128 85 215
Fragments arranged by size:
2617 215 128 85
Bme1580I G_kGCm'C
Cuts at: 0 344 390 1346 2494 2606 2950 3045
Size: 344 46 956 1148 112 344 95
Fragments arranged by size:
1148 956 344 344 112 95 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 775 3045
Size: 775 2270
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2826 2946 3045
Size: 2826 120 99
Fragments arranged by size:
2826 120 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 366 478 532 1042 1498 1933 2095
Size: 267 99 112 54 510 456 435 162
Cuts at: 2095 2149 2224 2287 2308 2341 2392 2506 3045
Size: 54 75 63 21 33 51 114 539
Fragments arranged by size:
539 510 456 435 267 162 114 112
99 75 63 54 54 51 33 21
Bpu10I CC'TnA_GC
Cuts at: 0 199 3045
Size: 199 2846
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 472 952 1561 2374 2920 3045
Size: 343 129 480 609 813 546 125
Fragments arranged by size:
813 609 546 480 343 129 125
BsaI GGTCTCn'nnnn_
Cuts at: 0 12 1546 1687 3045
Size: 12 1534 141 1358
Fragments arranged by size:
1534 1358 141 12
BsaAI yAC'GTr
Cuts at: 0 348 3045
Size: 348 2697
BsaHI Gr'CG_yC
Cuts at: 0 887 1873 3017 3032 3045
Size: 887 986 1144 15 13
Fragments arranged by size:
1144 986 887 15 13
BsaWI w'CCGG_w
Cuts at: 0 527 3045
Size: 527 2518
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 421 1252 1282 1436 1466 2023 2053
Size: 391 30 831 30 154 30 557 30
Cuts at: 2053 2902 2932 3045
Size: 849 30 113
Fragments arranged by size:
849 831 557 391 154 113 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 216 412 529 1216 1348 1351 2128 3045
Size: 216 196 117 687 132 3 777 917
Fragments arranged by size:
917 777 687 216 196 132 117 3
BseYI C'CCAG_C
Cuts at: 0 26 1514 1629 1949 2168 2339 2444 2464
Size: 26 1488 115 320 219 171 105 20
Cuts at: 2464 2735 2970 3045
Size: 271 235 75
Fragments arranged by size:
1488 320 271 235 219 171 115 105
75 26 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1698 2674 3045
Size: 757 941 976 371
Fragments arranged by size:
976 941 757 371
BsiEI CG_ry'CG
Cuts at: 0 2651 3045
Size: 2651 394
BsiHKAI G_wGCw'C
Cuts at: 0 344 1270 1846 2746 2809 3045
Size: 344 926 576 900 63 236
Fragments arranged by size:
926 900 576 344 236 63
BsmI GAATG_Cn'
Cuts at: 0 746 3045
Size: 746 2299
BsmBI CGTCTCn'nnnn_
Cuts at: 0 454 1996 3045
Size: 454 1542 1049
Fragments arranged by size:
1542 1049 454
Bsp1286I G_dGCh'C
Cuts at: 0 43 344 390 980 1270 1346 1846 2494
Size: 43 301 46 590 290 76 500 648
Cuts at: 2494 2606 2728 2746 2809 2836 2899 2950 3045
Size: 112 122 18 63 27 63 51 95
Fragments arranged by size:
648 590 500 301 290 122 112 95
76 63 63 51 46 43 27 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 461 1517 1851 2075 3045
Size: 461 1056 334 224 970
Fragments arranged by size:
1056 970 461 334 224
BsrBI CCG'CTC
Cuts at: 0 1416 2028 2085 2262 2277 2325 2361 2825
Size: 1416 612 57 177 15 48 36 464
Cuts at: 2825 3045
Size: 220
Fragments arranged by size:
1416 612 464 220 177 57 48 36
15
BsrFI r'CCGG_y
Cuts at: 0 2582 2988 3045
Size: 2582 406 57
Fragments arranged by size:
2582 406 57
BssHII G'CGCG_C
Cuts at: 0 2118 2216 2414 3045
Size: 2118 98 198 631
Fragments arranged by size:
2118 631 198 98
BssSI C'ACGA_G
Cuts at: 0 226 1263 1392 2155 3045
Size: 226 1037 129 763 890
Fragments arranged by size:
1037 890 763 226 129
BstAPI GCAn_nnn'nTGC
Cuts at: 0 348 1034 2772 2775 3045
Size: 348 686 1738 3 270
Fragments arranged by size:
1738 686 348 270 3
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1911 2676 3045
Size: 1911 765 369
Fragments arranged by size:
1911 765 369
BstYI r'GATC_y
Cuts at: 0 180 1469 1934 2663 3045
Size: 180 1289 465 729 382
Fragments arranged by size:
1289 729 465 382 180
Bsu36I CC'TnA_GG
Cuts at: 0 922 3045
Size: 922 2123
BtgI C'CryG_G
Cuts at: 0 257 1533 2348 3045
Size: 257 1276 815 697
Fragments arranged by size:
1276 815 697 257
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1225 2612 3045
Size: 1225 1387 433
Fragments arranged by size:
1387 1225 433
BtsI GCAGTG_nn'
Cuts at: 0 528 2983 3045
Size: 528 2455 62
Fragments arranged by size:
2455 528 62
DraI TTT'AAA
Cuts at: 0 110 3045
Size: 110 2935
DraIII CAC_nnn'GTG
Cuts at: 0 348 3045
Size: 348 2697
DrdI GACnn_nn'nnGTC
Cuts at: 0 892 3045
Size: 892 2153
EaeI y'GGCC_r
Cuts at: 0 222 426 627 1193 1484 1583 1644 2435
Size: 222 204 201 566 291 99 61 791
Cuts at: 2435 2588 2648 2990 3045
Size: 153 60 342 55
Fragments arranged by size:
791 566 342 291 222 204 201 153
99 61 60 55
EagI C'GGCC_G
Cuts at: 0 2648 3045
Size: 2648 397
EarI CTCTTCn'nnn_
Cuts at: 0 439 1355 3045
Size: 439 916 1690
Fragments arranged by size:
1690 916 439
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1666 3045
Size: 1666 1379
EcoICRI GAG'CTC
Cuts at: 0 1268 1844 2744 3045
Size: 1268 576 900 301
Fragments arranged by size:
1268 900 576 301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 366 391 478 532 649 970 988
Size: 267 99 25 87 54 117 321 18
Cuts at: 988 1042 1332 1498 1933 1951 2095 2149 2224
Size: 54 290 166 435 18 144 54 75
Cuts at: 2224 2287 2308 2341 2392 2506 2782 3045
Size: 63 21 33 51 114 276 263
Fragments arranged by size:
435 321 290 276 267 263 166 144
117 114 99 87 75 63 54 54
54 51 33 25 21 18 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 198 394 514 844 3045
Size: 198 196 120 330 2201
Fragments arranged by size:
2201 330 198 196 120
EcoO109I rG'GnC_Cy
Cuts at: 0 22 386 387 1342 1343 1542 1775 1792
Size: 22 364 1 955 1 199 233 17
Cuts at: 1792 2490 2491 3045
Size: 698 1 554
Fragments arranged by size:
955 698 554 364 233 199 22 17
1 1 1
EcoRV GAT'ATC
Cuts at: 0 870 1068 3045
Size: 870 198 1977
Fragments arranged by size:
1977 870 198
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 331 1018 3045
Size: 331 687 2027
Fragments arranged by size:
2027 687 331
HaeII r_GCGC'y
Cuts at: 0 498 921 1059 1229 1640 1876 2169 2184
Size: 498 423 138 170 411 236 293 15
Cuts at: 2184 2295 2301 2370 2544 2570 2624 2736 3008
Size: 111 6 69 174 26 54 112 272
Cuts at: 3008 3045
Size: 37
Fragments arranged by size:
498 423 411 293 272 236 174 170
138 112 111 69 54 37 26 15
6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 124 156 199 231 296 328 859
Size: 26 98 32 43 32 65 32 531
Cuts at: 859 891 937 969 2902 2934 3045
Size: 32 46 32 1933 32 111
Fragments arranged by size:
1933 531 111 98 65 46 43 32
32 32 32 32 32 26
HincII GTy'rAC
Cuts at: 0 2054 3045
Size: 2054 991
KasI G'GCGC_C
Cuts at: 0 1872 3045
Size: 1872 1173
MluI A'CGCG_T
Cuts at: 0 2056 3045
Size: 2056 989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1898 3045
Size: 1898 1147
MscI TGG'CCA
Cuts at: 0 629 1195 2590 3045
Size: 629 566 1395 455
Fragments arranged by size:
1395 629 566 455
MspA1I CmG'CkG
Cuts at: 0 127 1008 1437 1932 1953 2010 2109 2148
Size: 127 881 429 495 21 57 99 39
Cuts at: 2148 2613 2772 2908 3045
Size: 465 159 136 137
Fragments arranged by size:
881 495 465 429 159 137 136 127
99 57 39 21
NaeI GCC'GGC
Cuts at: 0 2584 2990 3045
Size: 2584 406 55
Fragments arranged by size:
2584 406 55
NarI GG'CG_CC
Cuts at: 0 1873 3045
Size: 1873 1172
NgoMIV G'CCGG_C
Cuts at: 0 2582 2988 3045
Size: 2582 406 57
Fragments arranged by size:
2582 406 57
NspI r_CATG'y
Cuts at: 0 1002 1032 2508 3045
Size: 1002 30 1476 537
Fragments arranged by size:
1476 1002 537 30
PasI CC'CwG_GG
Cuts at: 0 52 3045
Size: 52 2993
PflMI CCAn_nnn'nTGG
Cuts at: 0 2202 2878 3045
Size: 2202 676 167
Fragments arranged by size:
2202 676 167
PfoI T'CCnGG_A
Cuts at: 0 244 1400 1910 2021 3045
Size: 244 1156 510 111 1024
Fragments arranged by size:
1156 1024 510 244 111
PmlI CAC'GTG
Cuts at: 0 348 3045
Size: 348 2697
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 391 423 2023 2055 2809 2841 3045
Size: 391 32 1600 32 754 32 204
Fragments arranged by size:
1600 754 391 204 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 22 1542 3045
Size: 22 1520 1503
Fragments arranged by size:
1520 1503 22
PspOMI G'GGCC_C
Cuts at: 0 386 1342 2490 3045
Size: 386 956 1148 555
Fragments arranged by size:
1148 956 555 386
PstI C_TGCA'G
Cuts at: 0 395 407 1562 1624 2051 2141 2228 3045
Size: 395 12 1155 62 427 90 87 817
Fragments arranged by size:
1155 817 427 395 90 87 62 12
PvuII CAG'CTG
Cuts at: 0 127 1437 1953 2148 2613 2772 2908 3045
Size: 127 1310 516 195 465 159 136 137
Fragments arranged by size:
1310 516 465 195 159 137 136 127
SacI G_AGCT'C
Cuts at: 0 1270 1846 2746 3045
Size: 1270 576 900 299
Fragments arranged by size:
1270 900 576 299
SalI G'TCGA_C
Cuts at: 0 2052 3045
Size: 2052 993
SanDI GG'GwC_CC
Cuts at: 0 22 1542 3045
Size: 22 1520 1503
Fragments arranged by size:
1520 1503 22
SbfI CC_TGCA'GG
Cuts at: 0 395 1562 3045
Size: 395 1167 1483
Fragments arranged by size:
1483 1167 395
SfcI C'TryA_G
Cuts at: 0 391 403 1558 1620 2047 2137 2224 3045
Size: 391 12 1155 62 427 90 87 821
Fragments arranged by size:
1155 821 427 391 90 87 62 12
SfoI GGC'GCC
Cuts at: 0 1874 3045
Size: 1874 1171
SmlI C'TyrA_G
Cuts at: 0 358 487 967 1576 2389 2935 3045
Size: 358 129 480 609 813 546 110
Fragments arranged by size:
813 609 546 480 358 129 110
SphI G_CATG'C
Cuts at: 0 1032 2508 3045
Size: 1032 1476 537
Fragments arranged by size:
1476 1032 537
StuI AGG'CCT
Cuts at: 0 850 1526 2305 3045
Size: 850 676 779 740
Fragments arranged by size:
850 779 740 676
StyI C'CwwG_G
Cuts at: 0 890 2690 3008 3045
Size: 890 1800 318 37
Fragments arranged by size:
1800 890 318 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1102 3045
Size: 1102 1943
TatI w'GTAC_w
Cuts at: 0 377 3045
Size: 377 2668
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1436 1468 3045
Size: 1436 32 1577
Fragments arranged by size:
1577 1436 32
Tth111I GACn'n_nGTC
Cuts at: 0 1944 2855 3045
Size: 1944 911 190
Fragments arranged by size:
1944 911 190
XmnI GAAnn'nnTTC
Cuts at: 0 2038 3045
Size: 2038 1007
ZraI GAC'GTC
Cuts at: 0 3033 3045
Size: 3033 12
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII KasI
MluI MmeI MscI MspA1I NaeI NarI NgoMIV NspI
PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI
PvuII SacI SalI SanDI SbfI SfcI SfoI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AleI AscI AseI AsiSI
AvrII BaeI BcgI BciVI BfrBI BmgBI BmtI BsaBI
BsiWI BspEI BspHI BsrDI BsrGI BstBI BstEII BstZ17I
ClaI CspCI EcoRI FseI FspI FspAI HindIII HpaI
KpnI MfeI MslI NcoI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SapI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XbaI
XcmI XhoI XmaI