Restriction map for
pF1KA0522
(Linear) (Six-base) MAPSORT of: KIAA0522.seq Check: 3091 from: 1 to: 4464
KIAA0522.orf
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 482 4464
Size: 482 3982
AccI GT'mk_AC
Cuts at: 0 2910 4464
Size: 2910 1554
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1521 1915 1973 2562 4152 4464
Size: 1521 394 58 589 1590 312
Fragments arranged by size:
1590 1521 589 394 312 58
AfeI AGC'GCT
Cuts at: 0 943 4464
Size: 943 3521
AflIII A'CryG_T
Cuts at: 0 2450 2681 4464
Size: 2450 231 1783
Fragments arranged by size:
2450 1783 231
AgeI A'CCGG_T
Cuts at: 0 433 2352 4464
Size: 433 1919 2112
Fragments arranged by size:
2112 1919 433
AhdI GACnn_n'nnGTC
Cuts at: 0 2841 4464
Size: 2841 1623
AleI CACnn'nnGTG
Cuts at: 0 2353 4464
Size: 2353 2111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3445 4464
Size: 3445 1019
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 301 333 1271 1303 4464
Size: 301 32 938 32 3161
Fragments arranged by size:
3161 938 301 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 105 108 273 1951 2322 3151 3798 4464
Size: 105 3 165 1678 371 829 647 666
Fragments arranged by size:
1678 829 666 647 371 165 105 3
ApaI G_GGCC'C
Cuts at: 0 20 850 1457 1923 1982 2015 3650 3829
Size: 20 830 607 466 59 33 1635 179
Cuts at: 3829 3992 4153 4216 4397 4464
Size: 163 161 63 181 67
Fragments arranged by size:
1635 830 607 466 181 179 163 161
67 63 59 33 20
ApoI r'AATT_y
Cuts at: 0 720 2413 3023 3108 4464
Size: 720 1693 610 85 1356
Fragments arranged by size:
1693 1356 720 610 85
AvaI C'yCGr_G
Cuts at: 0 21 30 246 332 537 1627 1685 1726
Size: 21 9 216 86 205 1090 58 41
Cuts at: 1726 2150 2774 4464
Size: 424 624 1690
Fragments arranged by size:
1690 1090 624 424 216 205 86 58
41 21 9
AvrII C'CTAG_G
Cuts at: 0 2418 4464
Size: 2418 2046
BamHI G'GATC_C
Cuts at: 0 34 4219 4464
Size: 34 4185 245
Fragments arranged by size:
4185 245 34
BanI G'GyrC_C
Cuts at: 0 361 646 1246 1258 1638 2940 4023 4464
Size: 361 285 600 12 380 1302 1083 441
Fragments arranged by size:
1302 1083 600 441 380 361 285 12
BanII G_rGCy'C
Cuts at: 0 20 46 637 850 879 1030 1289 1457
Size: 20 26 591 213 29 151 259 168
Cuts at: 1457 1923 1982 2015 2104 3404 3619 3650 3829
Size: 466 59 33 89 1300 215 31 179
Cuts at: 3829 3913 3992 4153 4216 4240 4397 4464
Size: 84 79 161 63 24 157 67
Fragments arranged by size:
1300 591 466 259 215 213 179 168
161 157 151 89 84 79 67 63
59 33 31 29 26 24 20
BbeI G_GCGC'C
Cuts at: 0 365 650 1642 4464
Size: 365 285 992 2822
Fragments arranged by size:
2822 992 365 285
BbsI GAAGACnn'nnnn_
Cuts at: 0 1697 2771 2982 4464
Size: 1697 1074 211 1482
Fragments arranged by size:
1697 1482 1074 211
BbvCI CC'TCA_GC
Cuts at: 0 2392 3933 4464
Size: 2392 1541 531
Fragments arranged by size:
2392 1541 531
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2900 2934 3220 3254 4464
Size: 2900 34 286 34 1210
Fragments arranged by size:
2900 1210 286 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2323 4464
Size: 2323 2141
BglI GCCn_nnn'nGGC
Cuts at: 0 529 3755 3777 4016 4464
Size: 529 3226 22 239 448
Fragments arranged by size:
3226 529 448 239 22
BglII A'GATC_T
Cuts at: 0 3041 4464
Size: 3041 1423
BlpI GC'TnA_GC
Cuts at: 0 613 811 878 1116 1524 4464
Size: 613 198 67 238 408 2940
Fragments arranged by size:
2940 613 408 238 198 67
Bme1580I G_kGCm'C
Cuts at: 0 20 850 888 1263 1457 1923 1982 2015
Size: 20 830 38 375 194 466 59 33
Cuts at: 2015 3650 3829 3992 4026 4153 4216 4397 4464
Size: 1635 179 163 34 127 63 181 67
Fragments arranged by size:
1635 830 466 375 194 181 179 163
127 67 63 59 38 34 33 20
BmgBI CAC'GTC
Cuts at: 0 339 459 2451 4464
Size: 339 120 1992 2013
Fragments arranged by size:
2013 1992 339 120
BmrI ACTGGGnnnn_n'
Cuts at: 0 279 459 2009 2018 3801 3973 4257 4412
Size: 279 180 1550 9 1783 172 284 155
Cuts at: 4412 4464
Size: 52
Fragments arranged by size:
1783 1550 284 279 180 172 155 52
9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1539 4464
Size: 1539 2925
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 160 406 621 682 813 877 878
Size: 91 69 246 215 61 131 64 1
Cuts at: 878 1030 1370 1507 2088 2398 3181 3304 3347
Size: 152 340 137 581 310 783 123 43
Cuts at: 3347 3394 3775 4061 4118 4464
Size: 47 381 286 57 346
Fragments arranged by size:
783 581 381 346 340 310 286 246
215 152 137 131 123 91 69 64
61 57 47 43 1
Bpu10I CC'TnA_GC
Cuts at: 0 766 1036 1586 2392 3933 4154 4464
Size: 766 270 550 806 1541 221 310
Fragments arranged by size:
1541 806 766 550 310 270 221
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1399 2116 4464
Size: 1399 717 2348
Fragments arranged by size:
2348 1399 717
BsaI GGTCTCn'nnnn_
Cuts at: 0 274 2113 2771 3428 4464
Size: 274 1839 658 657 1036
Fragments arranged by size:
1839 1036 658 657 274
BsaHI Gr'CG_yC
Cuts at: 0 362 407 647 1639 4464
Size: 362 45 240 992 2825
Fragments arranged by size:
2825 992 362 240 45
BsaWI w'CCGG_w
Cuts at: 0 433 2352 2510 2624 4464
Size: 433 1919 158 114 1840
Fragments arranged by size:
1919 1840 433 158 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 301 331 370 400 624 654 1003
Size: 25 276 30 39 30 224 30 349
Cuts at: 1003 1033 1271 1301 1375 1405 1459 1489 2119
Size: 30 238 30 74 30 54 30 630
Cuts at: 2119 2149 2470 2500 3830 3860 4464
Size: 30 321 30 1330 30 604
Fragments arranged by size:
1330 630 604 349 321 276 238 224
74 54 39 30 30 30 30 30
30 30 30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 202 940 943 946 1198 1480 2133
Size: 148 54 738 3 3 252 282 653
Cuts at: 2133 2475 2659 3583 3591 3681 4464
Size: 342 184 924 8 90 783
Fragments arranged by size:
924 783 738 653 342 282 252 184
148 90 54 8 3 3
BseYI C'CCAG_C
Cuts at: 0 116 489 1481 1597 1944 1959 1965 2026
Size: 116 373 992 116 347 15 6 61
Cuts at: 2026 2222 2604 2692 3190 3854 3881 3902 3906
Size: 196 382 88 498 664 27 21 4
Cuts at: 3906 4124 4207 4464
Size: 218 83 257
Fragments arranged by size:
992 664 498 382 373 347 257 218
196 116 116 88 83 61 27 21
15 6 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 199 460 2872 3268 4066 4366 4464
Size: 184 15 261 2412 396 798 300 98
Fragments arranged by size:
2412 798 396 300 261 184 98 15
BsiHKAI G_wGCw'C
Cuts at: 0 637 772 2104 4464
Size: 637 135 1332 2360
Fragments arranged by size:
2360 1332 637 135
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2441 3597 4464
Size: 2441 1156 867
Fragments arranged by size:
2441 1156 867
Bsp1286I G_dGCh'C
Cuts at: 0 20 46 637 772 850 879 888 1030
Size: 20 26 591 135 78 29 9 142
Cuts at: 1030 1263 1289 1457 1923 1982 2015 2104 3404
Size: 233 26 168 466 59 33 89 1300
Cuts at: 3404 3619 3650 3829 3913 3992 4026 4153 4216
Size: 215 31 179 84 79 34 127 63
Cuts at: 4216 4240 4397 4464
Size: 24 157 67
Fragments arranged by size:
1300 591 466 233 215 179 168 157
142 135 127 89 84 79 78 67
63 59 34 33 31 29 26 26
24 20 9
BspEI T'CCGG_A
Cuts at: 0 2510 2624 4464
Size: 2510 114 1840
Fragments arranged by size:
2510 1840 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 74 188 281 482 992 3233 4464
Size: 74 114 93 201 510 2241 1231
Fragments arranged by size:
2241 1231 510 201 114 93 74
BsrBI CCG'CTC
Cuts at: 0 402 420 603 1104 1826 2331 2382 4464
Size: 402 18 183 501 722 505 51 2082
Fragments arranged by size:
2082 722 505 501 402 183 51 18
BsrDI GCAATG_nn'
Cuts at: 0 3236 4464
Size: 3236 1228
BsrFI r'CCGG_y
Cuts at: 0 433 528 649 806 1271 1622 2179 2352
Size: 433 95 121 157 465 351 557 173
Cuts at: 2352 2396 2426 3203 4010 4464
Size: 44 30 777 807 454
Fragments arranged by size:
807 777 557 465 454 433 351 173
157 121 95 44 30
BsrGI T'GTAC_A
Cuts at: 0 2684 4109 4464
Size: 2684 1425 355
Fragments arranged by size:
2684 1425 355
BssHII G'CGCG_C
Cuts at: 0 233 4464
Size: 233 4231
BssSI C'ACGA_G
Cuts at: 0 481 2611 3079 4464
Size: 481 2130 468 1385
Fragments arranged by size:
2130 1385 481 468
BstAPI GCAn_nnn'nTGC
Cuts at: 0 105 4464
Size: 105 4359
BstEII G'GTnAC_C
Cuts at: 0 3015 4464
Size: 3015 1449
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2022 2496 3739 3973 4464
Size: 2022 474 1243 234 491
Fragments arranged by size:
2022 1243 491 474 234
BstYI r'GATC_y
Cuts at: 0 34 2493 2946 3041 4219 4464
Size: 34 2459 453 95 1178 245
Fragments arranged by size:
2459 1178 453 245 95 34
Bsu36I CC'TnA_GG
Cuts at: 0 913 4464
Size: 913 3551
BtgI C'CryG_G
Cuts at: 0 56 204 228 586 595 618 1250 1808
Size: 56 148 24 358 9 23 632 558
Cuts at: 1808 1884 2489 3750 3972 4371 4457 4464
Size: 76 605 1261 222 399 86 7
Fragments arranged by size:
1261 632 605 558 399 358 222 148
86 76 56 24 23 9 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3410 3940 4464
Size: 3410 530 524
Fragments arranged by size:
3410 530 524
BtsI GCAGTG_nn'
Cuts at: 0 765 4099 4464
Size: 765 3334 365
Fragments arranged by size:
3334 765 365
ClaI AT'CG_AT
Cuts at: 0 1319 4464
Size: 1319 3145
EaeI y'GGCC_r
Cuts at: 0 589 686 3741 4012 4068 4225 4464
Size: 589 97 3055 271 56 157 239
Fragments arranged by size:
3055 589 271 239 157 97 56
EarI CTCTTCn'nnn_
Cuts at: 0 2151 2293 3001 3106 3699 3872 4464
Size: 2151 142 708 105 593 173 592
Fragments arranged by size:
2151 708 593 592 173 142 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 668 1063 3536 4464
Size: 668 395 2473 928
Fragments arranged by size:
2473 928 668 395
EcoICRI GAG'CTC
Cuts at: 0 635 2102 4464
Size: 635 1467 2362
Fragments arranged by size:
2362 1467 635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 160 406 621 682 813 877 878
Size: 91 69 246 215 61 131 64 1
Cuts at: 878 1030 1370 1507 1521 1915 1973 2088 2398
Size: 152 340 137 14 394 58 115 310
Cuts at: 2398 2562 3181 3304 3347 3394 3775 4061 4118
Size: 164 619 123 43 47 381 286 57
Cuts at: 4118 4152 4464
Size: 34 312
Fragments arranged by size:
619 394 381 340 312 310 286 246
215 164 152 137 131 123 115 91
69 64 61 58 57 47 43 34
14 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 184 4464
Size: 184 4280
EcoO109I rG'GnC_Cy
Cuts at: 0 16 17 222 846 847 951 1453 1723
Size: 16 1 205 624 1 104 502 270
Cuts at: 1723 1889 1919 1920 1978 1979 3646 3647 3795
Size: 166 30 1 58 1 1667 1 148
Cuts at: 3795 3826 3988 3989 4149 4150 4212 4213 4394
Size: 31 162 1 160 1 62 1 181
Cuts at: 4394 4464
Size: 70
Fragments arranged by size:
1667 624 502 270 205 181 166 162
160 148 104 70 62 58 31 30
16 1 1 1 1 1 1 1
1
EcoRI G'AATT_C
Cuts at: 0 720 2413 3108 4464
Size: 720 1693 695 1356
Fragments arranged by size:
1693 1356 720 695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3060 3666 4464
Size: 3060 606 798
Fragments arranged by size:
3060 798 606
FseI GG_CCGG'CC
Cuts at: 0 1626 4014 4464
Size: 1626 2388 450
Fragments arranged by size:
2388 1626 450
HaeII r_GCGC'y
Cuts at: 0 365 650 945 1642 4464
Size: 365 285 295 697 2822
Fragments arranged by size:
2822 697 365 295 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 33 65 624 656 1375 1407 1459
Size: 27 6 32 559 32 719 32 52
Cuts at: 1459 1491 1822 1854 2470 2502 2827 2859 3528
Size: 32 331 32 616 32 325 32 669
Cuts at: 3528 3560 3766 3798 4464
Size: 32 206 32 666
Fragments arranged by size:
719 669 666 616 559 331 325 206
52 32 32 32 32 32 32 32
32 32 27 6
HincII GTy'rAC
Cuts at: 0 2754 2911 4464
Size: 2754 157 1553
Fragments arranged by size:
2754 1553 157
KasI G'GCGC_C
Cuts at: 0 361 646 1638 4464
Size: 361 285 992 2826
Fragments arranged by size:
2826 992 361 285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1181 2014 2435 2856 3245 3257 3620 4464
Size: 1181 833 421 421 389 12 363 844
Fragments arranged by size:
1181 844 833 421 421 389 363 12
MscI TGG'CCA
Cuts at: 0 3743 4464
Size: 3743 721
MslI CAynn'nnrTG
Cuts at: 0 1165 2353 3737 3971 4464
Size: 1165 1188 1384 234 493
Fragments arranged by size:
1384 1188 1165 493 234
MspA1I CmG'CkG
Cuts at: 0 105 120 150 198 206 588 597 612
Size: 105 15 30 48 8 382 9 15
Cuts at: 612 775 819 882 1485 1854 2205 2586 2982
Size: 163 44 63 603 369 351 381 396
Cuts at: 2982 3906 4380 4464
Size: 924 474 84
Fragments arranged by size:
924 603 474 396 382 381 369 351
163 105 84 63 48 44 30 15
15 9 8
NaeI GCC'GGC
Cuts at: 0 530 651 1624 2398 4012 4464
Size: 530 121 973 774 1614 452
Fragments arranged by size:
1614 973 774 530 452 121
NarI GG'CG_CC
Cuts at: 0 362 647 1639 4464
Size: 362 285 992 2825
Fragments arranged by size:
2825 992 362 285
NcoI C'CATG_G
Cuts at: 0 1250 1884 2489 3972 4371 4464
Size: 1250 634 605 1483 399 93
Fragments arranged by size:
1483 1250 634 605 399 93
NdeI CA'TA_TG
Cuts at: 0 1293 4464
Size: 1293 3171
NgoMIV G'CCGG_C
Cuts at: 0 528 649 1622 2396 4010 4464
Size: 528 121 973 774 1614 454
Fragments arranged by size:
1614 973 774 528 454 121
NruI TCG'CGA
Cuts at: 0 503 4464
Size: 503 3961
NspI r_CATG'y
Cuts at: 0 1137 1164 2685 3096 3805 4464
Size: 1137 27 1521 411 709 659
Fragments arranged by size:
1521 1137 709 659 411 27
PasI CC'CwG_GG
Cuts at: 0 495 872 1617 3985 4464
Size: 495 377 745 2368 479
Fragments arranged by size:
2368 745 495 479 377
PciI A'CATG_T
Cuts at: 0 2681 4464
Size: 2681 1783
PflMI CCAn_nnn'nTGG
Cuts at: 0 2556 4464
Size: 2556 1908
PfoI T'CCnGG_A
Cuts at: 0 422 628 2375 3197 4464
Size: 422 206 1747 822 1267
Fragments arranged by size:
1747 1267 822 422 206
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 271 301 303 333 1271 1303 4464
Size: 271 30 2 30 938 32 3161
Fragments arranged by size:
3161 938 271 32 30 30 2
PpuMI rG'GwC_Cy
Cuts at: 0 222 1723 1889 4464
Size: 222 1501 166 2575
Fragments arranged by size:
2575 1501 222 166
PspOMI G'GGCC_C
Cuts at: 0 16 846 1453 1919 1978 2011 3646 3825
Size: 16 830 607 466 59 33 1635 179
Cuts at: 3825 3988 4149 4212 4393 4464
Size: 163 161 63 181 71
Fragments arranged by size:
1635 830 607 466 181 179 163 161
71 63 59 33 16
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1195 1227 4464
Size: 1195 32 3237
Fragments arranged by size:
3237 1195 32
PstI C_TGCA'G
Cuts at: 0 989 1879 2134 3147 4464
Size: 989 890 255 1013 1317
Fragments arranged by size:
1317 1013 989 890 255
PvuII CAG'CTG
Cuts at: 0 105 150 198 612 819 1485 1854 3906
Size: 105 45 48 414 207 666 369 2052
Cuts at: 3906 4464
Size: 558
Fragments arranged by size:
2052 666 558 414 369 207 105 48
45
SacI G_AGCT'C
Cuts at: 0 637 2104 4464
Size: 637 1467 2360
Fragments arranged by size:
2360 1467 637
SacII CC_GC'GG
Cuts at: 0 207 589 598 4464
Size: 207 382 9 3866
Fragments arranged by size:
3866 382 207 9
SalI G'TCGA_C
Cuts at: 0 2909 4464
Size: 2909 1555
SanDI GG'GwC_CC
Cuts at: 0 222 1723 1889 4464
Size: 222 1501 166 2575
Fragments arranged by size:
2575 1501 222 166
SapI GCTCTTCn'nnn_
Cuts at: 0 3106 4464
Size: 3106 1358
SbfI CC_TGCA'GG
Cuts at: 0 989 4464
Size: 989 3475
SexAI A'CCwGG_T
Cuts at: 0 3150 4464
Size: 3150 1314
SfcI C'TryA_G
Cuts at: 0 985 1875 2130 3143 4464
Size: 985 890 255 1013 1321
Fragments arranged by size:
1321 1013 985 890 255
SfoI GGC'GCC
Cuts at: 0 363 648 1640 4464
Size: 363 285 992 2824
Fragments arranged by size:
2824 992 363 285
SgrAI Cr'CCGG_yG
Cuts at: 0 649 2352 4464
Size: 649 1703 2112
Fragments arranged by size:
2112 1703 649
SmaI CCC'GGG
Cuts at: 0 23 32 248 334 539 1687 1728 4464
Size: 23 9 216 86 205 1148 41 2736
Fragments arranged by size:
2736 1148 216 205 86 41 23 9
SmlI C'TyrA_G
Cuts at: 0 1378 2095 4464
Size: 1378 717 2369
Fragments arranged by size:
2369 1378 717
SphI G_CATG'C
Cuts at: 0 3805 4464
Size: 3805 659
SrfI GCCC'GGGC
Cuts at: 0 248 4464
Size: 248 4216
StuI AGG'CCT
Cuts at: 0 2390 3682 4464
Size: 2390 1292 782
Fragments arranged by size:
2390 1292 782
StyI C'CwwG_G
Cuts at: 0 947 1250 1884 2418 2489 3335 3816 3972
Size: 947 303 634 534 71 846 481 156
Cuts at: 3972 4004 4371 4430 4464
Size: 32 367 59 34
Fragments arranged by size:
947 846 634 534 481 367 303 156
71 59 34 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 178 4041 4193 4302 4374 4464
Size: 178 3863 152 109 72 90
Fragments arranged by size:
3863 178 152 109 90 72
TatI w'GTAC_w
Cuts at: 0 2684 3572 4109 4464
Size: 2684 888 537 355
Fragments arranged by size:
2684 888 537 355
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3830 3862 4464
Size: 3830 32 602
Fragments arranged by size:
3830 602 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 616 837 3736 3751 4464
Size: 616 221 2899 15 713
Fragments arranged by size:
2899 713 616 221 15
XmaI C'CCGG_G
Cuts at: 0 21 30 246 332 537 1685 1726 4464
Size: 21 9 216 86 205 1148 41 2738
Fragments arranged by size:
2738 1148 216 205 86 41 21 9
XmnI GAAnn'nnTTC
Cuts at: 0 2437 4464
Size: 2437 2027
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApoI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NruI NspI PasI PciI PflMI
PfoI PpiI PpuMI PspOMI PsrI PstI PvuII SacI
SacII SalI SanDI SapI SbfI SexAI SfcI SfoI
SgrAI SmaI SmlI SphI SrfI StuI StyI TaqII
TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII ApaLI AscI AseI AsiSI
BaeI BclI BfrBI BmtI BsaAI BsaBI BsiEI BsiWI
BsmI BspHI BstBI BstZ17I CspCI DraI DraIII DrdI
EagI EcoRV FspI FspAI HindIII HpaI KpnI MfeI
MluI NheI NotI NsiI PacI PmeI PmlI PshAI
PsiI PspXI PvuI RsrII ScaI SfiI SgfI SnaBI
SpeI SspI SwaI Tth111I XbaI XhoI ZraI